Clear and present danger? The use of a yeast biosensor to monitor changes in the toxicity of industrial effluents subjected to oxidative colour removal treatments

Discharges of coloured effluents into surface waters provide conspicuous evidence of the impact of industry on the environment. The textile industry is an obvious candidate for sources of such discharges. Conventional treatment methods appear to alleviate this situation by removing colour, however the affect on toxicity is less obvious. The objective of this study was to examine the changes in effluent toxicity during the course of two alternative wastewater treatment methods, ozonation and electrochemical oxidation, using a novel toxicity biosensor, GreenScreen EM. The biosensor is capable of measuring both general acute toxicity (cytotoxicity), and more specifically genotoxicity, that is damage to a cell's DNA structure, replication or distribution, caused by substances that may be mutagenic and/or carcinogenic. The biosensor utilises a modified strain of the brewers yeast Saccharomyces cerevisiae, incorporating a gene encoding green fluorescent protein (GFP) linked to the inducible promoter of the DNA damage responsive RAD54 gene. Upon exposure to a genotoxin, the production of GFP is up-regulated in parallel with RAD54, and the resulting cellular fluorescence provides a measure of genotoxicity. Acute toxicity is simultaneously determined by monitoring relative total growth of the cell culture during incubation. The results presented in this paper show that a reduction in colouration does not necessarily correspond to a reduction in effluent toxicity.     

Evaluation of in situ steam‐injection processes for reduction of petroleum compounds within an abandoned canal

A conceptual approach of a novel application of in‐situ thermal processes that would either use a steam injection process or a steam/surfactant injection process was considered to remediate petroleum contaminated sediment residing in an abandoned canal. Laboratory tests were conducted in an attempt to volatilize or mobilize contaminants of concern (selected polycyclic aromatic hydrocarbons [PAHs]) from the contaminated sediment into a phase that could be physically removed. The processes were operated above ambient temperature and pressure in an attempt to increase the removal of the contaminants of concern from the sediment. The ability of both the steam injection process and the steam/surfactant process to remove PAHs from the sediment was considered ineffective; as only two of the seventeen selected PAHs (naphthalene and C1 naphthalene) were associated with a percentage mass reduction greater than 34% for both treatments (four trials). The steam/surfactant injection process generally resulted in higher reductions than the steam injection process, but had larger variances within the two trials using the treatment type. This preliminary evaluation suggests that steam‐based injection processes for removing petroleum contamination from this canal sediment, using the surfactants selected, equipment set‐up, and operating conditions studied, would be considered ineffective. © 2010 Wiley Periodicals, Inc. *

1 This article is a U.S. Government work and, as such, is in the public domain of the United States of America.

     

Introduction to the special issue: Place,identity and environmental behaviour

Research on identity has proliferated in recent decades, particularly within environmental psychology; the physical environment has been shown to have strong connections to a sense of self, and identity has proved to be an important mediator of behaviour. The concept of identity has been defined and measured, however, in a wide variety of ways. The goal of this special issue is to present some of the recent work tying identity to place and behaviour. In our opening essay we describe some of the distinctions among approaches to identity at different levels of specificity and scale and suggest some criteria to determine meaningful sources of identity, including impacts on cognitive processing, emotional responses, and behaviour. Although a monolithic framework is neither practical nor desirable, we encourage greater conceptual and methodological integration in future research on the interconnections among place, identity, and behaviour.     

Evaluation of the vulnerability to contamination of drinking water systems for rural regions in Québec,Canada

The aim of this paper is to describe a method for evaluating the vulnerability of drinking water systems to contamination, in particular in rural regions used intensively for agriculture. To do so, various indicators were developed to represent the source to tap multi-barrier approach for drinking water safety. These indicators correspond to four barriers: source susceptibility to contamination; water treatment efficiency; distribution system management; and, overall management of water quality. The indicators were classified, regrouped and weighted within a model based on a multi-criteria analysis. The method was developed and applied to 39 municipal water systems of rural Quebec, Canada. The model obtained can be used for planning purposes to prioritise water systems requiring improvements.     

Studies of corrosion tendency of drinking water in the distribution system at the University of Benin

Ensemble of corrosion indices was combined to study the corrosion tendency of the drinking water supply at the University of Benin, Nigeria. The experimental results were analysed in terms of three corrosion indices-Langelier Index, Ryznar Index and Larson–Skold Index. According to the evaluation, the Langelier Index ranged from −5.569 to −3.684, Ryznar Index was between 13.340 and 16.418 while the Larson–Skold Index was between 1.191 and 31.750. Results indicated that the water may be corrosive. A regression of these indices on iron concentration (ppm) showed that Langelier Index, Ryznar Index and Larson–Skold Index have R ² of 0.5868, 0.6577 and 0.7063, respectively. The positive correlation between iron levels and the corrosion indices suggested that iron levels were directly related to increase in corrosion tendency.     

Vegetarian Meat: Could Technology Save Animals and Satisfy Meat Eaters?

Between people who unabashedly support eating meat and those who adopt moral vegetarianism, lie a number of people who are uncomfortably carnivorous and vaguely wish they could be vegetarians. Opposing animal suffering in principle, they can ignore it in practice, relying on the visual disconnect between supermarket meat and slaughterhouse practices not to trigger their moral emotions. But what if we could have the best of both worlds in reality—eat meat and not harm animals? The nascent biotechnology of tissue culture, originally researched for medical applications, holds out just such a promise. Meat could be grown in vitro without killing animals. In fact, this technology may not just be an intriguing option, but might be our moral obligation to develop.     

Microbes do not follow the elevational diversity patterns of plants and animals

The elevational gradient in plant and animal diversity is one of the most widely documented patterns in ecology and, although no consensus explanation exists, many hypotheses have been proposed over the past century to explain these patterns. Historically, research on elevational diversity gradients has focused almost exclusively on plant and animal taxa. As a result, we do not know whether microbes exhibit elevational gradients in diversity that parallel those observed for macroscopic taxa. This represents a key knowledge gap in ecology, especially given the ubiquity, abundance, and functional importance of microbes. Here we show that, across a montane elevational gradient in eastern Peru, bacteria living in three distinct habitats (organic soil, mineral soil, and leaf surfaces) exhibit no significant elevational gradient in diversity (r2<0.17, P>0.1 in all cases), in direct contrast to the significant diversity changes observed for plant and animal taxa across the same montane gradient (r2>0.75, P<0.001 in all cases). This finding suggests that the biogeographical patterns exhibited by bacteria are fundamentally different from those of plants and animals, highlighting the need for the development of more inclusive concepts and theories in biogeography to explain these disparities.     

Clusters of community exposure to coastal flooding hazards based on storm and sea level rise scenarios—implications for adaptation networks in the San Francisco Bay region

Regional Environmental Change - Sea level is projected to rise over the coming decades, further increasing the extent of flooding hazards in coastal communities. Efforts to address potential...     

Effects of urbanization on the population structure of freshwater turtles across the United States

Landscape‐scale alterations that accompany urbanization may negatively affect the population structure of wildlife species such as freshwater turtles. Changes to nesting sites and higher mortality rates due to vehicular collisions and increased predator populations may particularly affect immature turtles and mature female turtles. We hypothesized that the proportions of adult female and immature turtles in a population will negatively correlate with landscape urbanization. As a collaborative effort of the Ecological Research as Education Network (EREN), we sampled freshwater turtle populations in 11 states across the central and eastern United States. Contrary to expectations, we found a significant positive relationship between proportions of mature female painted turtles (Chrysemys picta) and urbanization. We did not detect a relationship between urbanization and proportions of immature turtles. Urbanization may alter the thermal environment of nesting sites such that more females are produced as urbanization increases. Our approach of creating a collaborative network of scientists and students at undergraduate institutions proved valuable in terms of testing our hypothesis over a large spatial scale while also allowing students to gain hands‐on experience in conservation science.