First trimester screening cannot predict adverse outcomes yet

The use of first trimester screening to detect aneuploidy has become an integral part of prenatal care. The application of similar screening algorithms to identify women at the highest risk for other adverse pregnancy outcomes in the first trimester could potentially have a major clinical impact. There has been much investigation into the ability to identify patients early in pregnancy at high risk for adverse pregnancy outcomes who may benefit from further surveillance and/or intervention. For this to be the case, however, as is true of any useful screening test, effective interventions need to be available. Unfortunately, for fetal growth restriction and stillbirth, no such interventions exist short of delivery. For preeclampsia, low dose aspirin has been demonstrated to be of benefit in specific subgroups. For preterm birth, although there are efficacious treatments, first trimester serum markers or cervical length measurements do not add significantly beyond historical or demographic factors, in prediction of preterm birth. Given the current evidence, first trimester screening, via serum or ultrasound markers, does not have sufficiently high enough positive predictive values for the development of preeclampsia, fetal growth restriction, preterm birth or stillbirth. In order to develop effective screening algorithms for adverse pregnancy outcomes in the first trimester, understanding the heterogeneous phenotype of these complications and the underlying pathophysiology is needed. © 2014 John Wiley & Sons, Ltd.     

斜坡区和隆起区断裂密集带差异性及其对油气分布的控制作用

此文以霸县凹陷文安斜坡和饶阳凹陷留楚构造为研究对象,对比了斜坡区和隆起区断裂密集带的特征及其对不同构造部位油气分布的控制作用。在三维地震资料、钻井资料和已发现油气藏特征的基础上,利用断裂密集带识别方法和分类方案,识别出文安斜坡和留楚构造的断裂密集带,并对其进行了分类。对比两种构造部位断裂密集带发育的差异性及对油气分布的控制作用,认为隆起区(留楚构造)相对于斜坡区(文安斜坡)断裂密集带发育数量较少,规模大,斜坡区断裂密集带主要以顺向背形断裂密集带为主,而隆起区主要以屋脊背形断裂密集带为主,隆起区油气主要分布在顺向背形断裂密集带内,斜坡区主要分布在屋脊背形断裂密集带内部及靠近生烃中心的边部。     

A salt-free pickling regime for hides and skins using oxazolidine

During the last few decades new materials and technologies have being studied and applied in order to reduce the ecological impact of leather production. Severe restrictions imposed by the pollution control authorities on the disposal of chromium, total dissolved solids and chlorides in tannery effluents have forced the tanners and researchers to look for eco-benign pickling and tanning processes. In this study, we would focus on the novel process development of pickling with the presence of oxazolidine based product. Investigations indicated that oxazolidine is a potential material to replace neutral salts in pickling and, realize high chrome exhaustion in the subsequential chrome tanning.     

Preimplantation genetic diagnosis for single gene disorders: experience with five single gene disorders

We report our experience of 14 preimplantation genetic diagnosis (PGD) cycles in eight couples carrying five different single gene disorders, during the last 18 months. Diagnoses were performed for myotonic dystrophy (DM), cystic fibrosis (CF) [ΔF508 and exon 4 (621+1 G>T)], fragile X and CF simultaneously, and two disorders for which PGD had not been previously attempted, namely neurofibromatosis type 2 (NF2) and Crouzon syndrome. Diagnoses for single gene disorders were carried out on ideally two blastomeres biopsied from Day 3 embryos. A highly polymorphic marker was included in each diagnosis to control against contamination. For the dominant disorders, where possible, linked polymorphisms provided an additional means of determining the genotype of the embryo hence reducing the risk of misdiagnosis due to allele dropout (ADO). Multiplex fluorescent polymerase chain reaction (F-PCR) was used in all cases, followed by fragment analysis and/or single-stranded conformation polymorphism (SSCP) for genotyping. Embryo transfer was performed in 13 cycles resulting in one biochemical pregnancy for CF, three normal deliveries (a twin and a singleton) and one early miscarriage for DM and a singleton for Crouzon syndrome. In each case the untransferred embryos were used to confirm the diagnoses performed on the biopsied cells. The results were concordant in all cases. The inclusion of a polymorphic marker allowed the detection of extraneous DNA contamination in two cells from one case. Knowing the genotype of the contaminating DNA allowed its origin to be traced. All five pregnancies were obtained from embryos in which two blastomeres were biopsied for the diagnosis. Our data demonstrate the successful strategy of using multiplex PCR to simultaneously amplify the mutation site and a polymorphic locus, fluorescent PCR technology to achieve greater sensitivity, and two-cell biopsy to increase the efficiency and success of diagnoses. Copyright © 2002 John Wiley & Sons, Ltd.     

Laboratory evaluation of kinetic parameters for lake sediment denitrification models

Laboratory experiments using the acetylene blockage technique showed that denitrification rates in sediments from Lake Okeechobee, Florida, are described by Michaelis-Menten nitrate dependence and Arrhenius temperature dependence (E_act = 15.5 kcal, but do not appear to be affected by seasonal differences in the supply of carbon substrates in this eutrophic lake. Peak nitrate concentrations in the sediments are in the range of the half-saturation constant, and thus, for a given temperature, k_d varies by a factor of approximately two over the typical range of nitrate concentrations. Most diagenetic sediment denitrification models employ a single-valued rate constant k_d, to describe nitrate dependence. However, the use of a constant k_d for eutrophic lake sediments may result in a significantly erroneous estimate of annual denitrification loss.     

Ideal dominance distributions: a test using red-winged blackbirds (Agelaius phoeniceus)

Summary The ideal dominance distribution model predicts that competition between individuals of a species for territories will result in socially dominant individuals acquiring territories in higher quality habitat than their subordinates. Although the dispersion and relative reproductive success of male red-winged blackbirds (Agelaius phoeniceus) across habitats in eastern Ontario appears to conform to the ideal dominance distribution model, data from a study of three captive groups comprised of males from both high (marsh) and low (upland field) quality habitats failed to support the prediction that males from marsh habitat are dominant to those from upland habitat. Contrary to the prediction males from uplands were generally dominant to males from marshes. We found a significant positive correlation between dominance and both increased epaulet size and increased body size. Controlling for these positive effects, upland males remained generally dominant to marsh males. Measurements of independent samples of males from both habitats indicated that the overall distribution of males does not conform to ideal dominance. We suggest that the strong between-year territory fidelity shown by male red-winged blackbirds and chance events when they initially acquire territories may contribute to this lack of conformity.