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761.
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763.
Lillian Y. F. Hsu MD Sara Kaffe Edmund C. Jenkins Lita Alonso Peter A. Benn Karen David Kurt Hirschhorn Ernest Lieber Alan Shanske Lawrence R. Shapiro Edward Schutta Dorothy Warburton 《黑龙江环境通报》1992,12(7):555-573
Currently, accepted protocol which has been developed at the Prenatal Diagnosis Laboratory of New York City (PDL) requires that when a chromosome abnormality is found in one or more cells in one flask, another 20–40 cells must be examined from one or two additional flasks. Chromosome mosaicism is diagnosed only when an identical abnormality is detected in cells from two or more flasks. In a recent PDL series of 12 000 cases studied according to this protocol, we diagnosed 801 cases (6.68 per cent) of single-cell pseudomosaicism (SCPM), 126 cases (1.05 per cent) of multiple-cell pseudomosaicism (MCPM), and 24 cases (0.2 per cent) of true mosaicism. Pseudomosaicism (PM) involving a structural abnormality was a frequent finding (2/3 of SCPM and 3/5 of MCPM), with an unbalanced structural abnormality in 55 per cent of SCPM and 24 per cent of MCPM. We also reviewed all true mosaic cases (a total of 50) diagnosed in the first 22000 PDL cases. Of these 50 cases, 23 were sex chromosome mosaics and 27 had autosomal mosaicism; 48 cases had numerical abnormalities and two had structural abnormalities. Twenty-five cases of mosaicism were diagnosed in the first 20 cells from two flasks, i.e., without additional work-up, whereas the other 25 cases required extensive work-up to establish a diagnosis (12 needed additional cell counts from the initial two culture flasks; 13 required harvesting a third flask for cell analysis). Our data plus review of other available data led us to conclude that rigorous efforts to diagnose true mosaicism have little impact in many instances, and therefore are not cost-effective. On the basis of all available data, a work-up for potential mosaicism involving a sex chromosome aneuploidy or structural abnormality should have less priority than a work-up for a common viable autosomal trisomy. We recommend revised guidelines for dealing with (1) a numerical versus a structural abnormality and (2) an autosomal versus a sex chromosome numerical aneuploidy. Emphasis should be placed on autosomes known to be associated with phenotypic abnormalities. These new guidelines, which cover both flask and in situ methods, should result in more effective prenatal cytogenetic diagnosis and reduced patient anxiety. 相似文献
764.
Goal and Scope
Polycyclic aromatic hydrocarbons (PAHs) are one group of persistent organic pollutants which are ubiquitous distributed in soils, even in rural areas. After their release into the atmosphere, transportation and deposition, they tend to accumulate in topsoils and sediments. Similar distribution pattern of PAHs in atmospheric deposition, soil samples as well as sediment samples indicate a close relationship between atmospheric input and accumulation of PAHs in the terrestrial environment. The intention of this paper was to estimate the time when precautionary values of the German law of soil protection will be exceeded in rural areas. Furthermore, current soil concentrations will be linked to the historical record of PAHs by means of enrichment factors.Methods
The historical record of the atmospheric deposition rates of PAHs can be obtained from investigation of sediment cores. Based thereon, enrichment factors of PAHs in the environment were calculated. With these enrichment factors it was possible to estimate the recent PAH concentration in soils in rural areas from currently measured annual deposition rates. Furthermore, concentrations of PAHs in soils and deposition rates can be used to calculate the time when precautionary values will be reached.Results and Conclusion
PAH deposition rates have been decreasing since about 1960 by a factor of 2–3, but stabilized during the last decade on a level high above pre-industrial time. Thus, further enrichment of PAH in topsoils has to be expected. Actual deposition rates in connection with historical enrichment factors allow to determine the median concentrations of PAHs in rural soils. The time when precautionary values will be reached was calculated to about 300 Years.Outlook
The database to predict the further development of atmospheric deposition rates is very weak. There was a lack of validated methods concerning direct measurements of atmospheric POP deposition. Meanwhile, a national draft of a standard base on time-integrated passive sampling exists. This method can be implemented to establish a combined soil and deposition-monitoring program in order to assess the risk of further accumulation of POPs in soils. 相似文献765.
766.
The level of fluctuating asymmetry (FA), defined as random deviations from perfect bilateral symmetry, is assumed to reflect the developmental instability (DI) of an organism. Because environmental and genetic stress may increase DI, FA has been used to assess the level of stress experienced by, for example, fish. In this study, left–right asymmetry of lapillar otoliths was related to nutritional condition as estimated from RNA/DNA ratios, in order to investigate the utility of FA to detect feeding-related stress in Baltic cod, Gadus morhua L., larvae. Cod larvae in intermediate and good nutritional condition showed similar values of FA, and these were more symmetric than for larvae in poor condition. As increased levels of FA were restricted to larvae in a condition comparable to that of larvae experiencing at least 3 days of starvation in laboratory experiments, it is suggested that FA is an insensitive indicator of short-term feeding success of larval cod. However, FA can be used to reveal severely starved larva populations and probably also populations that have been subjected to prolonged sub-optimal feeding conditions. 相似文献
767.
Skin cancers associated with ingesting of arsenic have been documented since the 19th century. A study in the southwestern coastal area of Taiwan where people drank well water containing arsenic is generally recognised as providing the best data available for quantifying the risk, and the US Environmental Protection Agency (EPA) used these data to conduct a risk assessment of arsenic ingestion. However, the lowest exposure category in the Taiwan study included arsenic levels up to 290 µg L–1, which is nearly six times higher than the current EPA maximum contaminant level (MCL), 50 µg L–1. Therefore, the EPA risk assessment model extrapolated data on high-level exposures to generate risk estimates for low-level exposures. To evaluate the validity of this model, we conducted a quantitative review of epidemiological studies observing arsenic exposures below 290 µg L–1. A ratio of the likelihood of the EPA model being inappropriate to that of it being appropriate was calculated for each study population as a measurement of the validity of the EPA model. Although existing human data on low-level exposures are limited, the review suggested that the EPA model is unlikely to be able to predict the risk of skin cancer accurately when the arsenic exposure level is between 170 and 270 µg L–1. 相似文献
768.
Peter Schausberger 《Behavioral ecology and sociobiology》2007,62(1):119-125
Kin recognition, the biased treatment of conspecific individuals based on genetic relatedness, is a widespread phenomenon
in animals. The most common mechanisms mediating kin recognition are prior association (familiarity) and phenotype matching.
Recognition based on prior association allows identifying familiar individuals. Recognition based on phenotype matching is
an extension of prior-association-based recognition and allows identifying familiar and unfamiliar individuals due to a shared
phenotypic trait. I investigated which of the two mechanisms is used by cannibalistic juvenile predatory mites, Phytoseiulus persimilis. Protonymphs that were associated with either siblings or non-kin discriminated familiar and unfamiliar larvae and preferentially
cannibalized the latter irrespective of genetic relatedness. In contrast, despite previous association with either siblings
or non-kin, protonymphs did not discriminate unfamiliar sibling and unfamiliar non-kin larvae. Association in the larval stage
therefore mediated kin recognition based on familiarity, but not phenotype matching in cannibalistic P. persimilis protonymphs. Furthermore, in the presence of a familiar prey individual, sibling cannibalism occurred significantly sooner
than non-kin cannibalism. This quick sibling cannibalism may have been the consequence of preferential association of siblings
and/or may indicate the occurrence of an alternative cannibal phenotype. I discuss the adaptive significance of prior-association-based
recognition for P. persimilis juveniles and emphasize the ability of P. persimilis to use multiple recognition mechanisms in dependence of the ontogeny and the ecological context. 相似文献
769.
Siddiqui ZA Singh LP 《Journal of environmental biology / Academy of Environmental Biology, India》2005,26(1):117-122
Glasshouse experiments were conducted twice to assess the ash amendments (0, 20, and 40% with soil), a phosphate solubilizing microorganism Pseudomonas striata and a root-nodule bacterium Rhizobium sp on the reproduction of root-knot nematode Meloidogyne incognita and on the growth and transpiration of pea. Amendments of fly ash with soil had no effect on transpiration. However, M. incognita reduced the rate of transpiration from 1st week onward after inoculation while inoculation of Rhizobium sp and P. striata increased transpiration from 1st week onward after their inoculation both in nematode inoculated and uninoculated plants. Increase in transpiration was greater when both organisms were inoculated together. Addition of 20 and 40% fly ash with soil was beneficial for plant growth both in nematode inoculated and uninoculated plants. Inoculation of above organisms also increases plant growth of nematode inoculated and uninoculated plants in different fly ash soil mixture but increase in growth was greater when both organisms were inoculated together. Use of 20% fly ash increased galling and nematode multiplication over plants grown in without fly ash while 40% fly ash had adverse effect on galling and nematode multiplication. Rhizobium sp had greater adverse effect on galling and nematode multiplication than P. striata. Use of both organisms together had greater adverse effect on galling and nematode multiplication than caused by either of them alone. Highest reduction in galling and nematode multiplication was observed when both organisms were used in 40% fly ash amended soil. However, highest transpiration was observed in plants without nematodes and inoculated with both organisms together both in with or without fly ash amended soil. 相似文献
770.
Growth characteristics of native and invasive Caulerpa taxifolia from coastal eastern Australia were compared in warm (22–25°C) and cool (15–18°C) water in two laboratory experiments. Measurements
of biomass, stolon length, mean frond length and numbers of fronds and stolon meristems, demonstrated that the growth of native
and invasive C. taxifolia in warm water was up to ten times greater than in cool water. Growth rates differed substantially among locations over 9 weeks,
but for most variables, the growth of native C. taxifolia was greater than the growth of invasive C. taxifolia in warm water. In cool water, there was little difference in growth of invasive versus native C. taxifolia. The morphology of C. taxifolia changed considerably in response to temperature, such that native and invasive samples that were morphologically distinct
at the start of the experiment became indistinguishable after 69 days in warm water. We concluded there was little evidence
that the C. taxifolia which recently invaded temperate estuaries in New South Wales could spread faster in cool water than could native C. taxifolia from Queensland. Native tropical C. taxifolia appears tolerant of a wide range of environmental conditions and seems capable of surviving and becoming invasive in temperate
estuaries. 相似文献