Production and structure prediction of amylases from Chlorella vulgaris

Environmental Science and Pollution Research - Amylases are enzymes required for starch degradation and are naturally produced by many microorganisms. These enzymes are used in several fields such...     

Water and soil contaminated by arsenic: the use of microorganisms and plants in bioremediation

Environmental Science and Pollution Research - Owing to their roles in the arsenic (As) biogeochemical cycle, microorganisms and plants offer significant potential for developing...     

Probabilistic modeling of young children's overall lead exposure in France: integrated approach for various exposure media

OBJECTIVES: Emissions of lead into the environment (and thus its environmental concentrations) have decreased in recent years. We sought to estimate the overall lead exposure of children aged 6 months to 6 years (the population group most exposed and most sensitive to lead) in France through the various media (air, food, water, soils, and dust) and the respective contributions of each medium to the total dose. We have focused on the general population, leaving aside specific risk factors such as deteriorated lead paints. METHODS: We used the most recent French intake data for food and water, and a daily ingested quantity selected from the literature for soils and dust. Contamination data came from the first total diet study of contaminant levels in France (2000-2001), from regulatory testing of tap water (2004), a literature review of lead in urban soils, and a pilot study (2005) of lead in dust. Air quality monitoring measurements showed that the contribution of air could now be safely ignored. Weekly exposure doses were estimated with Monte Carlo simulations. RESULTS: Median weekly exposure dose was 7.5 microg/kg bw.week for children aged 6 months to 3 years and 4.7 for those aged from 3-6 years. 95th percentiles were 13.5 and 8.7 microg/kg bw.week. Exposure came mainly from food. The principal uncertainties are associated with quantification limits in food and water, representativeness and traceability of tap water samples, and absence of recent data about urban soil contamination. CONCLUSIONS: These results differ quite notably from earlier estimates and highlight the need, especially for policy-making purposes, to update exposure measurements for this metal.     

From IBM to IPM: Using individual-based models to design the spatial arrangement of traps and crops in integrated pest management strategies

The development of alternative pest-control strategies based on the spatial design of cropping systems requires a thorough understanding of the spatial links between the pest and its environment. Mechanistic models, especially individual-based models (IBMs), are powerful tools for integrating key behaviours, such as habitat selection and dispersal, with spatial heterogeneity. In this paper, we used an IBM calibrated and evaluated from real data to represent the spatial dynamics of the banana weevil in relation to the cropping system. We considered crop fragmentation and mass trapping as tools for suppressing pest numbers. Our simulation results showed that manipulating crop residues in the area surrounding each pheromone trap greatly improved trap efficiency. For an intensive banana plantation in fallow, traps were most effective when situated at the transition zone between banana area and fallow so as to maximize the trapping of weevils escaping the fallow. The model also showed that weevil numbers decreased when fragmentation of banana plantations was reduced.     

Climate Change Impacts and Uncertainties on Spring Flooding of Lake Champlain and the Richelieu River

The source of the Richelieu River is Lake Champlain, located between the states of New York, Vermont, and Québec. In 2011, the lake and the Richelieu River reached historical flood levels, raising questions about the influence of climate change on the watershed. The objectives of this work are to model the hydrology of the watershed, construct a reservoir model for the lake and to analyze flooding trends using climate simulations. The basin was modeled using the HSAMI lumped conceptual model from Hydro‐Québec with a semi‐distributed approach in order to estimate the inflows into Lake Champlain. The discharge at the Richelieu River was computed by using a mass balance equation between the inputs and outputs of Lake Champlain. Future trends were estimated over the 2041‐2070 and 2071‐2100 periods using a large number of outputs from general circulation models and regional climate models downscaled with constant scaling and daily translation methods. While there is a certain amount of uncertainty as to future trends, there is a decreasing tendency in the magnitude of the mean spring flood. A flood frequency analysis showed most climate projections indicate the severity of most extreme spring floods may be reduced over the two future periods although results are subject to a much larger uncertainty than for the mean spring flood. On the other hand, results indicate summer‐fall extreme events such as caused by hurricane Irene in August 2011 may become more frequent in the future.     

Rates of apical septal extension of Desmophyllum dianthus: effect of association with endolithic photo-autotrophs

It has been hypothesized that endolithic photo-autotrophs inside the skeleton of cold-water corals may have a mutualistic relationship with the coral host positively affecting coral calcification. This study investigated the effect of endolithic photo-autotrophs on the apical septal extension of the cold-water coral Desmophyllum dianthus at Fjord Comau, southern Chile (42.41°–42.15°S, 72.5°W). The fluorescent staining agent calcein was used to document the linear apical extension of septae for a period of one and a half years between 2006 and 2007. The results showed a severe reduction in extension rates associated with the presence of endolithic photo-autotrophs. Infested individuals grew about half as fast as non-infested polyps with a median value of 1.18 μm day^?1 compared to 2.76 μm day^?1. Contrary to the initial hypothesis, these results point toward a parasitic relationship between D. dianthus and its endolithic photo-autotrophs potentially impairing coral fitness. However, further data on physiological parameters and other aspects of the calcification process are necessary to confirm these findings.