Bioavailability of calcium from sweetpotato and soy flour supplemented diets in hamsters

The bioavailability of calcium from two varieties of sweetpotatoes and supplementation of sweetpotatoes with soy flour was investigated in hamsters using plasma calcium concentration and femur calcium content as indicators. Five different diets were fed to five groups of animals for 28 days. There was no significant difference in plasma calcium concentrations of hamsters in all the diet groups. However, the femur calcium content of hamsters with transgenic sweetpotato flour (TSPF) and parent nontransgenic (from which transgenic was produced) sweetpotato flour (NTSPF) diets was significantly higher than that of the transgenic sweetotato flour supplemented with soy flour (TSPF + SF) and parent nontransgenic sweetpotato flour supplemented with soy flour (NTSPF + SF) diets. The relative bioavailability of calcium from the control (100%), TSPF+SF (30%), NTSPF+SF (23%), TSPF (57%) and NTSPF (46%) indicated that sweetpotatoes could be the better source of calcium, however, supplementation with soy flour might reduce the bioavailability of calcium.     

Influence and persistence of phorate and carbofuran insecticides on microorganisms in rice field

An experiment was conducted in microplots (4 m x 4 m) with two insecticides, phorate and carbofuran at rates of 1.5 and 1.0 kga.i.ha(-1) respectively, to investigate its effect on the population and distribution of bacteria, actinomycetes and fungi as well as the persistence of the insecticidal residues in rhizosphere soils of rice (Oryza sativa L., variety IR-50). Application of the insecticides stimulated the population of bacteria, actinomycetes and fungi in the rhizosphere soils, and the stimulation was more pronounced with phorate as compared to carbofuran. Both the insecticides did not have marked effect on the numbers of Streptomyces and Nocardia in the rhizosphere soils. However, the growth of Bacillus, Escherichia, Flavobacterium, Micromonospora, Penicillium, Aspergillus and Trichoderma with phorate and that of Bacillus, Corynebacterium, Flavobacterium, Aspergillus and Phytophthora with carbofuran were increased. On the other hand, the numbers of Staphylococcus, Micrococcus, Fusarium, Humicola and Rhizopus under phorate and Pseudomonas, Staphylococcus, Micrococcus, Klebsiella, Fusarium, Humicola and Rhizopus under carbofuran were inhibited. Both the insecticides persisted in the rhizosphere soil for a short period of time and the rate of dissipation of carbofuran was higher than that of phorate in the soil depicting the half-life (T1/2) 9.1 and 10.4 days, respectively.     

Risk assessment and pathway study of arsenic in industrially contaminated sites of Hyderabad: a case study

Different areas in the industrial region of Patancheru near Hyderabad, Andhra Pradesh (A.P), India are contaminated with high concentration of arsenic, which is attributed to industrial source like veterinary chemicals, pharmaceuticals, pesticide industries, etc. Fourteen villages of this area of Patancheru were assessed for arsenic contamination by collecting samples of water (surface and ground), soil, fodder, milk, and vegetables. The total arsenic content in the whole blood, urine, hair, and nails of the residents showing arsenical skin lesions and other clinical manifestations were also studied. To understand the bioavailability of arsenic in this environment and its possible entry into human food chain, speciation studies of arsenic was carried out and the results are presented in this paper.     

CADMIUM ACCUMULATION AND TOXICITY IN SUBMERGED PLANT HYDRILLA VERTICILLATA (l.f.) ROYLE

Cadmium accumulation and its toxicity in relation tochlorophyll, protein, cysteine contents and in vivo nitrate reductaseactivity were studied under controlled conditions in Hydrillaverticillata, a submerged commonly occurring macrophyte. Plants weresubjected to six different concentrations of Cd ranging from 1.0 to 25.0 µM for 24, 48, 72 and 168 h. Tissue Cd concentration was maximum (13.71 µmoles/g dw) at 25 µM background concentration. At this concentration, a decrease of approximately 79 and 72% was found in chlorophyll and protein content. In vivo nitrate reductase activity was stimulated at 1.0 µM; however, the activity gradually declined beyond this concentration. Exposure to various cadmium concentrations resulted in an increase in cysteine content of the plant.     

Effect of combustion variables on PAHs emission from incineration of cellulose waste filters from acrylic industry

Incineration of cellulose waste filter from acrylic industry showed the presence of 13–16 polycyclic aromatic hydrocarbons (PAHs) from the list of 16 priority pollutants with an airflow rate of 1, 2, 3, and 4 L min^???1 in laboratory scale quartz tube vertical incinerator at 700–1,000°C at an interval of 100°C. The amount of total 16 PAHs increases with the increase in temperature with airflow rate of 1 L min^???1 and was found to be 9.4 times at 1,000°C than at 700°C. Studies at 800–1,000°C showed the decrease in total 16 PAHs with increase in airflow rate from 1 to 2 L min^???1. The amount of total 16 PAHs increases at 700, 800, and 1,000°C with increase in airflow rate from 2–4 L min^???1. At 900°C, amount of 16 PAHs decreases with increase in flow rate from 1 to 3 and increases at 4 L min^???1. The lesser amount of 2A PAHs was found at 700–900°C with airflow rates of 1–3 L min^???1, while less amount of 2B PAHs was found at 700°C and 800°C (with airflow rate of 1–2 L min^???1), at 900°C (with airflow rate of 1–3 L min^???1) and at 1,000°C (with airflow rate of 3 L min^???1). However, the sum total of 2A and 2B PAHs were found to be less at 700–900°C with airflow rate of 1–2 L min^???1.     

Kinetic and equilibrium isotherm studies for the adsorptive removal of Brilliant Green dye from aqueous solution by rice husk ash

The present study deals with the adsorption of Brilliant Green (BG) on rice husk ash (RHA). RHA is a solid waste obtained from the particulate collection equipment attached to the flue gas lines of rice husk fired boilers. Batch studies were performed to evaluate the influences of various experimental parameters like initial pH (pH0), contact time, adsorbent dose and initial concentration (C0) on the removal of BG. Optimum conditions for BG removal were found to be pH0 approximately 3.0, adsorbent dose approximately 6 g L(-1) of solution and equilibrium time approximately 5 h for the C0 range of 50-300 mg L(-1). Adsorption of BG followed pseudo-second-order kinetics. Intra-particle diffusion does not seem to control the BG removal process. Equilibrium isotherms for the adsorption of BG on RHA were analyzed by Freundlich, Langmuir, Redlich-Peterson (R-P), Dubnin-Radushkevich (D-R), and Temkin isotherm models using a non-linear regression technique. Langmuir and R-P isotherms were found to best represent the data for BG adsorption onto RHA. Adsorption of BG on RHA is favourably influenced by an increase in the temperature of the operation. Values of the change in entropy (DeltaS0) and heat of adsorption (DeltaH0) for BG adsorption on RHA were positive. The high negative value of change in Gibbs free energy (DeltaG0) indicates the feasible and spontaneous adsorption of BG on RHA.     

Effect of gamma-irradiated sludge on the growth and yield of rice (Oryza sativa L. var. GR-3)

The effects of gamma-irradiated sludge on the growth and yield of rice (Oryza sativa L. var. GR-3) in pot cultures have been studied. Compared to plants grown only in soil, shoot length, root length, fresh weight, dry weight, total proteins, total soluble sugars, starch and chlorophyll content of plants grown in soil supplemented with unirradiated or gamma-irradiated sludge were found to be significantly increased. Irradiation of sludge significantly stimulated the linear growth of shoot and root systems as well as fresh and dry weights of plants, compared to those grown in soil containing unirradiated sludge. There was also an improvement in the grain yield (weight of seed) when plants were grown in soil supplemented with irradiated sludge. The results obtained suggest that the gamma-irradiated sewage sludge can be beneficially recycled for agricultural uses.