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181.
为了验证PM2.5进入肺部后,在肺巨噬细胞清除PM2.5组分中是否发生细胞焦亡效应,本研究以THP-1细胞作为人巨噬细胞模型,以不同浓度PM2.5暴露于THP-1细胞,采用CCK-8法检测细胞存活率,试剂盒检测乳酸脱氢酶(LDH)释放水平,荧光显微镜下观察细胞PI染色情况,流式细胞仪检测Annexin V/PI染色,蛋白免疫印迹(Western blotting)检测焦亡相关蛋白NLRP3、ASC、caspase-1、GSDMD的表达,以及ELISA法检测IL-1β、IL-18的分泌等来检测PM2.5暴露诱导THP-1细胞的焦亡发生和炎症因子释放水平.结果显示:THP-1细胞暴露于PM2.5后,细胞存活率的降低与PM2.5暴露浓度的增加呈正相关;THP-1暴露PM2.548 h后,细胞胀大并呈气泡状;与空白对照(PBS)组相比,PM2.5暴露组与阳性对照组(1.0μg·mL-1LPS+5.0... 相似文献
182.
The ozone pollution at ground level in rural and urban areas has been a long-standing problem in the world. This paper focuses on estimating self-affined nature of nonlinearity of ground-level peak ozone time series, which is analyzed by two nonlinear fractal statistical methods, including R/S analysis and BDS test. To explore the underlying structure of ozone observations at ground level and improve the forecasting capacity in urban region, practical implementation was assessed by a case study via collecting and analyzing the monitoring data at Chaojhou and Zenwu in the Kaohsiung metropolitan region, Taiwan. Based on R/S analysis, the time series can be identified as persistent and long-memory processes with Hurst exponents of both about 0.75. In addition, the V statistics specifies possible fluctuation cycle lengths of 32, 170, and 420 day simultaneously. Such results are consistent with the regional meteorological conditions leading to help characterize the regional scale ozone behavioral trend. Furthermore, the BDS test results confirm a strong nonlinearity of both time series associated with these two cities. Yet in both cases, nonlinearity implies chaos. The R/S analysis and BDS test provide strong evidence for nonlinearity and fractality of ozone time series due to noisy chaos, and we could not rule out the possibility of deterministic chaos in tropospheric ozone system. 相似文献
183.
利用化工污染物能抑制荧光素酶催化发光反应这一特性,对水质中6种化工化合物分别进行毒性测试,建立一种快速检测水质中化工污染物毒性的生物学方法。实验结果表明,对苯二酚、4-氨基酚、苯胺、氯仿、盐酸邻联甲苯胺和硫柳汞的EC50值分别为:5.563、10.225、20.236、38.432、8.827和0.6 mg/L,6种污染物毒性大小依次为:硫柳汞>对苯二酚>盐酸邻联甲苯胺>4-氨基酚>苯胺>氯仿,相关系数>0.95。该方法为进一步研究基于荧光素酶反应体系检测水质中化工污染物综合毒性的生物传感器方法奠定基础。 相似文献
184.
Nocturnal chemistry can play an important role in determining the initial morning conditions for daytime chemistry in urban areas. However, the impact on daytime O3 levels is difficult to assess as the suppression of vertical trace gas transport leads to highly altitude dependent nocturnal chemistry, in particular with respect to the removal and conversion of nitrogen oxides (NOx) and volatile organic compounds (VOC). One-dimensional (1-D) chemical transport model calculations for different nighttime vertical stabilities and different ozone formation regimes (i.e. NOx- vs. VOC-sensitive) were performed assuming a 1000 m high daytime boundary layer and a growing nocturnal boundary layer reaching 200 m height at the end of the night. Exclusion of NO3 chemistry from the model leads to daytime O3 concentration changes from ?4% to +16% for different O3 sensitivities. In all cases strong nocturnal vertical concentration profiles of NOx, O3, NO3 and N2O5 and a dependence of these profiles on vertical stability were found at night. The nocturnal NOx loss averaged over the lowest 1000 m changes by 9–24% for different vertical stabilities and ozone sensitivities. The impact of nocturnal vertical stability leads to 7–12% difference in O3 concentration in the morning and ~0–2.5% in the afternoon. 相似文献
185.
186.
Yongxin Zhang Yanping Yang Yue Gong Ying Wang Xuecheng Wu Zhiying Zhou Weiguo Weng 《环境科学学报(英文版)》2023,123(1):212-221
Vessel emissions have contributed a great deal to air quality deterioration in China. Hence,the Chinese government has promulgated a series of stringent emission regulations. It is in this context that vessel emission control technology research is in full swing. In particular,during the 13th Five-Year Plan, the air pollution control technology of vessels has greatly improved. Vessel emission control has followed two main governance routes: source emission reduction and aftertreatment technology... 相似文献
187.
Xue Xiaoxiao Weng Yujing Yang Shicheng Meng Shihang Zhang Zhengting Yi Guiyun Zhang Yulong 《Environmental science and pollution research international》2021,28(13):15883-15889
Environmental Science and Pollution Research - Using copper nitrate trihydrate as the copper source, TiO2@Cu-MOF nanocomposites were prepared by a one-step crystallization method, and the effect of... 相似文献
188.
Zhijia Miao Wei Zeng Shuying Wang Yongzhen Peng Guihua Cao Dongchen Weng Guisong Xue Qing Yang 《环境科学学报(英文版)》2014,26(2):264-273
Temperature is an important physical factor, which strongly influences biomass and metabolic activity. In this study, the effects of temperature on the anoxic metabolism of nitrite(NO-2) to nitrous oxide(N2O) by polyphosphate accumulating organisms, and the process of the accumulation of N2O(during nitrite reduction), which acts as an electron acceptor, were investigated using 91% ± 4% Candidatus Accumulibacter phosphatis sludge. The results showed that N2O is accumulated when Accumulibacter first utilize nitrite instead of oxygen as the sole electron acceptor during the denitrifying phosphorus removal process. Properties such as nitrite reduction rate, phosphorus uptake rate, N2O reduction rate, and polyhydroxyalkanoate degradation rate were all influenced by temperature variation(over the range from 10 to 30°C reaching maximum values at 25°C). The reduction rate of N2O by N2O reductase was more sensitive to temperature when N2O was utilized as the sole electron acceptor instead of NO2, and the N2O reduction rates, ranging from 0.48 to 3.53 N2O-N/(hr·g VSS), increased to 1.45 to 8.60 mg N2O-N/(hr·g VSS). The kinetics processes for temperature variation of 10 to 30°C were(θ1 = 1.140–1.216 and θ2= 1.139–1.167). In the range of 10°C to 30°C, almost all of the anoxic stoichiometry was sensitive to temperature changes. In addition, a rise in N2O reduction activity leading to a decrease in N2O accumulation in long term operations at the optimal temperature(27°C calculated by the Arrhenius model). 相似文献
189.
新一代分子标记技术——AFLP 总被引:31,自引:0,他引:31
AFLP(Amplifiedfragmentlengthpolymorphism)是近年来发展起来的最有效的新一代分子标记技术.本文对AFLP的发生发展、基本原理、反应程序,在植物分子生物学研究中的应用以及方法本身的优缺点进行了介绍和评述. 相似文献
190.