盐生杜氏藻3-磷酸甘油脱氢酶不同结构域蛋白的表达纯化及其抗体的制备与分析

盐生杜氏藻(Dunaliella salina)是迄今为止世界上发现的最耐盐的真核光合生物,而甘油是盐藻用于调节细胞内外渗透压变化的关键调渗物质.3-磷酸甘油脱氢酶(GPDH)是盐生杜氏藻甘油合成途径中的关键酶.与已经报道的其它物种的GPDH基因不同,盐生杜氏藻3-磷酸甘油脱氢酶(Ds.GPDH)基因具有两个独立的功能结构域,即丝氨酸磷酸化酶结构域(SGPP domain)和3-磷酸甘油脱氢酶结构域(GPD domain).本实验在已克隆的Ds.GPDH2基因的基础上,以含GPDH2全基因序列的T质粒载体(pMD18-T-GPDH2)为模板,PCR扩增分别得到两个单结构域片段(SGPP与GPD),以及双结构域片段(GPDH),构建了pET32a-SGPP、pET32a-GPD和pET32a-GPDH三种原核表达载体,并在大肠杆菌中成功表达.纯化蛋白制备抗原,制备出3个多克隆抗体.Western Blot和交叉反应分析显示通过重组蛋白制备的多克隆抗体具有很高的特异性.图7参16     

Geochemistry of the barkinite liptobiolith (Late Permian) from the Jinshan Mine,Anhui Province,China

This paper discusses the geochemistry and mineralogy of the barkinite liptobiolith of the Late Permian age from the Jinshan Mine, Guangde County, Anhui Province, China. Samples were examined using inductively coupled plasma-mass spectroscopy, X-ray fluorescence, cold-vapor atomic absorption spectrometry, ion-selective electrode, sequential chemical extraction, scanning electron microscopy equipped with energy-dispersive X-ray, and optical microscopy. The coal is a medium-ash and high-sulfur resource. Minerals in the coal are composed of kaolinite, pyrite, calcite, and quartz. Pyrite and calcite are derived from seawater during peat accumulation. Quartz in the coal is of authigenic origin. Part of the kaolinite is from a land-source region, and part occurs as cell-fillings and is of authigenic origin. The results also indicate that the barkinite liptobiolith contains some toxic elements in high concentrations. Elements including Li, Be, Si, Sc, Ti, V, Cr, Fe, Ga, Se, Y, Zr, Mo, the rare earth elements (REEs), W, Hg, Tl, Pb, Th, and U in the coals are enriched in the barkinite liptobiolith. Results of sequential chemical extraction showed that Li, Sc, Ti, Cr, Y, Zr, REEs, and Th in the coal mainly occur as silicates, while Be and W are related to organic matter. Pyrite is the dominant source of S, Mo, Hg, Tl, and Pb. Gallium only occurs in silicate, and U and V occur both in organic and silicate associations.     

CdI42-·Fe(phen)32+离子对1,2-二氯乙烷萃取光度法测定水中痕量镉的研究

研究萃取光度法测定痕量Cd的最佳实验条件,结果表明,λ_max=512nm,ε=2.42×10⁶L·mol^-1·cm^-1,相对标准偏差为2.6%(自来水)和3.1%(河水).水样中Cd的含量在0-1μg/25ml范围内服从比尔定律。应用于自来水及河水中Cd的测定,结果令人满意。     

香港大气气溶胶组成与特征

选用香港空气质量监测网中11个监测站1990～1994年间TSP（总悬浮粒子）和RSP（可吸入悬浮粒子）的监测数据,分析香港大气气溶胶的化学组成与时空变化。香港大气气溶胶的浓度较低,其浓度的季节变化主要受气候变化的影响。C是大气气溶胶中最重要的化学成分,它支配着气溶胶的季节和空间变化。SO^2-₄、NH⁺₄和NO^-₃的浓度很低,空间分布很均匀,其季节变化主要受东亚季风控制。海洋气溶胶主要以较大的颗粒形式存在,它的来源稳定且空间分布较均匀。扬尘浓度主要受降雨和湿度的影响。V和Ni浓度的季节变化与燃料油消耗量的季节变化相同,其空间的变化反映了当地工业窑炉排放对香港气溶胶的影响     

炭膜及其在环境领域中的应用

介绍了一种新型的无机分离膜———炭膜 ,讨论了炭膜的孔结构等基本性质 ,综述了这种新材料在气体分离、饮用水净化和废水处理等方面的应用 ,并结合目前炭膜的研究开发现状 ,分析了制约其发展的因素及相应的解决方法     

Expanding the valorization of waste mushroom substrates in agricultural production: progress and challenges

Environmental Science and Pollution Research - Waste mushroom substrate (WMS) generated in large quantities from mushroom production process has caused severe environmental pollution. As a...     

Impacts of leachate of landfill on the groundwater hydrochemistry and size distributions and heavy metal components of colloids: a case study in NE China

Environmental Science and Pollution Research - Colloids associated with heavy metals are ubiquitous in contaminated groundwater; waste accumulation at imperfectly sealed landfills can produce large...     

Uptake kinetics and interaction of selenium species in tomato (Solanum lycopersicum L.) seedlings

Environmental Science and Pollution Research - Selenite and selenate are two main selenium (Se) forms absorbed by plants. The comparative effects of selenite and/or selenate on Se uptake and...     

全自动驾驶模式下站台门智能安全监控系统研究

全自动驾驶是一种先进的轨道交通列车运行控制技术,在全自动驾驶模式下,轨道交通列车运维对站台门的安全提出了更高的要求。本文在分析站台门在全自动驾驶模式下的安全监控需求的基础上,从站台门的运行外部环境和站台门自身的运行参数两方面出发,建立站台门智能安全监控系统,实时全面掌握站台门的安全状态,基于BIM技术和自适应学习技术,对站台门的安全状态进行预测,防范于未然并及时做好维修计划调整。该系统能让站台门的安全处于主动控制状态,减少安全隐患。     

Chemical modification of montan resin by peracetic acid and agricultural application of its modified products

ABSTRACT

Montan resin (MR) is a by-product produced during the refinement process of montan wax extracted from lignite and has no usage yet. Chemical modification is an effective method to change the material property for expanding or converting the application area of the material itself. Our previous study found that the high hydrophobicity of MR is the primary limiting factor for its utilization in agriculture. Based on this point, this study attempted to chemically modify MR using the oxidation of peracetic acid, resulting that the MR hydrophilicity was significantly improved, and a water-soluble product (WSP) was obtained. The optimized oxidation conditions of MR, including the reaction temperature (X₁), reaction time (X₂), weight ratio of oxidant and montan resin (X₃), and oxidant concentration (X₄), were determined using single-factor experiments and response surface analysis. The modification degree was evaluated using elemental and oil-water partition coefficient analyses, infrared (IR) spectroscopy, and gas chromatography-mass spectrometry (GC-MS), revealing that the oil-water partition coefficient of the modified product decreased and that the number of chemical constituents with oxygen-containing functional groups clearly increased after modification. Furthermore, the WSP was tested its effects on germination and seedling growth of the wheat seed. Compared with the control group, the WSP showed a promoting effect on the growth and germination of wheat. The WSP concentrations of 600 mg·L^?1 and 300 mg·L^?1 had the most substantial effect on the root and seedling growth of wheat, respectively.

Implications: Montan resin, a useless by-product produced from crude montan wax, was chemically modified via oxidation of peracetic acid. Its hydrophilicity was significantly improved, and a water-soluble product was obtained after the chemical modification. The optimized oxidation conditions of montan resin were determined using single-factor experiments and response surface analysis. The amount of chemical constituents with oxygen-containing functional groups increased in the modified products after modification, as determined by IR and GC-MS analysis, among other methods. The water-soluble modified product showed an obvious effect in promoting growth and germination of wheat at 600 mg·L-1 and 300 mg·L-1, respectively.