Degradation of hop bitter acids by fungi

Nine fungal strains related to: Trametes versicolor, Nigrospora oryzae, Inonotus radiatus, Crumenulopsis sororia, Coryneum betulinum, Cryptosporiopsis radicicola, Fusarium equiseti, Rhodotorula glutinis and Candida parapsilosis were tested for their ability to degrade humulones and lupulones. The best results were obtained for T. versicolor culture, in which humulones and lupulones were fully degraded after 4days of incubation in the dark or after 36h in the light. The experiments were performed on a commercial hop extract and on sterilized spent hops.     

Microbially mediated cadmium sorption/desorption processes in soil amended with sewage sludge

A multi-compartment system was used to study the importance of microorganisms for Cd desorption from soil amended with sewage sludge and simultaneous resorption of the mobilized metal by soil constituents. Using this system made it possible to study the participation of microorganisms (Arthrobacter, Trichoderma), montmorillonite, humic acids, and iron oxides in resorption of the released Cd. A filter-sterilized water extract of root-free soil of pH 6.7 (RF) or RF supplemented with glucose (RFG) were used to mobilize Cd from soil at 14 degrees C in 48 h. Cadmium found in those extracts after 48-h incubation was recognized as bioavailable. Changes in pH values and enrichment of soil extracts with organic acids and siderophores resulted from microbial growth. RFG with lower pH and a higher content of ligands mobilized, on average, 40% of Cd introduced with sewage sludge amended soil, whereas RF mobilized only 20% of it. Sequential extractions of Cd at time 0 and Cd remaining in soil showed that RFG had mobilized Cd mostly from the fraction bound with Fe and Mn oxides. Microbial biomass accounted for only up to 3.4% (w/w) of the soil constituents used in the experiments but resorbed 25% of mobilized Cd. The chemical composition of mobilizing soil extracts and the solid-to-mobilizing-extracts volume ratio had a significant effect on the amount of bioavailable Cd. The results of the study suggest that microbial metabolites were involved in Cd mobilization, while the biomass of microorganisms was involved in Cd resorption as a biosorbent.     

Thermally Induced Reaction of Potato Starch with Thiourea

Thermally induced reaction of thiourea with potato starch was carried out with involvement of either conventional or microwave heating. Under applied reaction conditions conventional heating provided a higher degree of transformation. The course of reaction was complex. Thiourea reacted with starch but the reaction products underwent further decompositions. Dextrins contaminated with isothiocyanates were obtained. Not necessarily they underwent alpha-amylolysis more readily than original starch.     

The effect of thymol and its derivatives on reactions generating reactive oxygen species

The effects of thymol (TOH), thymoquinone (TQ) and dithymoquinone (TQ2) on the reactions generating reactive oxygen species (ROS) such as superoxide anion radical (O2*-), hydroxyl radical (HO*) and singlet oxygen (1O2) were tested using the chemiluminescence (CL) and spectrophotometry methods. All tested compounds acted as scavengers of various ROS. The rate constant of 1O2-dimols quenching by thymol was calculated.     

Anaerobic co-digestion of swine and poultry manure with municipal sewage sludge

The anaerobic digestion of municipal sewage sludge (SS) with swine manure (SM) and poultry manure (PM) was undertaken. It was found that a mixture of sewage sludge with a 30% addition of swine manure gave around 400 dm³/kgVS of biogas, whereas the maximal biogas yield from ternary mixture (SS:SM:PM = 70:20:10 by weight) was only 336 dm³/kgVS. An inhibition of methanogenesis by free ammonia was observed in poultry manure experiments. The anaerobic digestion was inefficient in pathogen inactivation as the reduction in the number of E. coli an Enterobacteriaceae was only by one logarithmic unit. A substantial portion of pathogens was also released into the supernatant.     

Family and species as determinants modulating mineral composition of selected wild-growing mushroom species

It has been known since the 1970s that differences exist in the profile of element content in wild-growing mushroom species, although knowledge of the role of mushroom species/families as determinants in the accumulation of diverse element remains limited. The aim of this study was to determine the content of 63 mineral elements, divided into six separate groups in the fruit bodies of 17 wild-growing mushroom species. The mushrooms, growing in widely ranging types of soil composition, were collected in Poland in 2018. Lepista nuda and Paralepista gilva contained not only the highest content of essential major (531 and 14,800 mg kg⁻¹, respectively of Ca and P) and trace elements (425 and 66.3 mg kg⁻¹, respectively of Fe and B) but also a high content of trace elements with a detrimental health effect (1.39 and 7.29 mg kg⁻¹, respectively of Tl and Ba). A high content of several elements (Al, B, Ba, Bi, Ca, Er, Fe, Mg, Mo, P, Sc, Ti or V) in L. nuda, Lepista personata, P. gilva and/or Tricholoma equestre fruit bodies belonging to the Tricholomataceae family suggests that such species may be characterised by the most effective accumulation of selected major or trace elements. On the other hand, mushrooms belonging to the Agaricaceae family (Agaricus arvensis, Coprinus comatus and Macrolepiota procera) were characterised by significant differences in the content of all determined elements jointly, which suggests that a higher content of one or several elements is mushroom species-dependent.

Graphical abstract

     

Interaction of different forms of graphene with chicken embryo red blood cells

With the rapid development of graphene synthesis and functionalization approaches, graphene and its related derivatives have shown great potential in many applications in material science, including biomedical applications. Several in vitro and in vivo studies clearly showed no definitive risks, while others have indicated that graphene might become health hazards. In this study, we explore the biocompatibility of graphene-related materials with chicken embryo red blood cells (RBC). The hemolysis assay was employed to evaluate the in vitro blood compatibility of reduced graphene, graphene oxide, and reduced graphene oxide, because these materials have recently been used for biomedical applications, including injectable graphene-related particles. This study investigated structural damage, ROS production and hemolysis of chicken embryo red blood cells. Different forms of graphene, when incubated with chicken embryo RBC, were harmful to cell structure and induced hemolysis.     

Modelling deposition and air concentration of reduced nitrogen in Poland and sensitivity to variability in annual meteorology

The relative contribution of reduced nitrogen to acid and eutrophic deposition in Europe has increased recently as a result of European policies which have been successful in reducing SO(2) and NO(x) emissions but have had smaller impacts on ammonia (NH(3)) emissions. In this paper the Fine Resolution Atmospheric Multi-pollutant Exchange (FRAME) model was used to calculate the spatial patterns of annual average ammonia and ammonium (NH(4)(+)) air concentrations and reduced nitrogen (NH(x)) dry and wet deposition with a 5 km × 5 km grid for years 2002-2005. The modelled air concentrations of NH(3) and dry deposition of NH(x) show similar spatial patterns for all years considered. The largest year to year changes were found for wet deposition, which vary considerably with precipitation amount. The FRAME modelled air concentrations and wet deposition are in reasonable agreement with available measurements (Pearson's correlation coefficients above 0.6 for years 2002-2005), and with spatial patterns of concentrations and deposition of NH(x) reported with the EMEP results, but show larger spatial gradients. The error statistics show that the FRAME model results are in better agreement with measurements if compared with EMEP estimates. The differences in deposition budgets calculated with FRAME and EMEP do not exceed 17% for wet and 6% for dry deposition, with FRAME estimates higher than for EMEP wet deposition for modelled period and lower or equal for dry deposition. The FRAME estimates of wet deposition budget are lower than the measurement-based values reported by the Chief Inspectorate of Environmental Protection of Poland, with the differences by approximately 3%. Up to 93% of dry and 53% of wet deposition of NH(x) in Poland originates from national sources. Over the western part of Poland and mountainous areas in the south, transboundary transport can contribute over 80% of total (dry + wet) NH(x) deposition. The spatial pattern of the relative contribution of national sources to total deposition of NH(x) may change significantly due to the general circulation of air.