Effect of 3 years' free-air exposure to elevated ozone on mature Norway spruce (Picea abies (L.) Karst.) needle epicuticular wax physicochemical characteristics

We examined the effect of ozone (O₃) on Norway spruce (Picea abies) needle epicuticular wax over three seasons at the Kranzberg Ozone Fumigation Experiment. Exposure to 2× ambient O₃ ranged from 64.5 to 74.2 μl O₃ l⁻¹ h AOT40, and 117.1 to 123.2 nl O₃ l⁻¹ 4th highest daily maximum 8-h average O₃ concentration. The proportion of current-year needle surface covered by wax tubes, tube aggregates, and plates decreased (P = 0.011) under 2× O₃. Epistomatal chambers had increased deposits of amorphous wax. Proportion of secondary alcohols varied due to year (P = 0.004) and O₃ treatment (P = 0.029). Secondary alcohols were reduced by 9.1% under 2× O₃. Exposure to 2× O₃ increased (P = 0.037) proportions of fatty acids by 29%. Opposing trends in secondary alcohols and fatty acids indicate a direct action of O₃ on wax biosynthesis. These results demonstrate O₃-induced changes in biologically important needle surface characteristics of 50-year-old field-grown trees.     

Perinatal dioxin exposure, cytochrome P-450 activity, liver functions and thyroid hormones at follow-up after 7-12 years

OBJECTIVES: Prenatal and lactational exposure to Dutch "background" dioxin levels may cause health effects spanning many years. In addition, perinatal studies have shown a relationship between dioxin exposure and thyroid disturbance. To assess the later health effects of prenatal and lactational dioxin exposure on liver function we measured plasma ALAT and ASAT levels amongst our longitudinal cohort, as was done perinatally and at 2(1/2) years. The children underwent a caffeine loading test to determine CYP1A2 activity. To assess the later effects on thyroid function we measured plasma TSH and FT4. STUDY DESIGN: A longitudinal cohort of 37 healthy children (age 7-12, mean 8.2 years), with documented prenatal and lactational dioxin exposure, ingested 3mg caffeine/kg BW 6h prior to blood withdrawal. Paraxanthine/caffeine molar ratio, ALAT, ASAT, TSH and FT4 were determined in venous blood. RESULTS: Linear regression of ASAT and ALAT revealed no relation with prenatal and lactational dioxin exposure. No correlation was found between the paraxanthine/caffeine molar ratio and prenatal and lactational dioxin exposure. Linear regression of TSH and FT4 revealed no relation with prenatal and lactational dioxin exposure. CONCLUSION: This follow-up has shown a normalisation of previously abnormal ALAT and ASAT levels, indicating a transient effect. CYP1A2 activity, measured by means of a caffeine-loading test, revealed no correlation with the prenatal and lactational exposures. A normalisation of previously abnormal thyroid hormone homeostasis was seen, also possibly indicating a transient effect. This study provides new data on long-term follow-up after perinatal dioxin exposure to background levels of dioxins.     

Sugar beet ( L.) growth reduction caused by hydrochar is related to nitrogen supply

Hydrothermal carbonization allows rapid conversion of biomass into a carbon-rich, lignite-like product (hydrochar). It is assumed to have beneficial effects on soil properties and plant growth, but detailed studies are lacking, especially in the field. The objective of our study was to investigate the effect of hydrochar incorporated into arable soils on soil mineral nitrogen (N) content and sugar beet growth. In 2010-2011, a field and a pot trial were conducted. Hydrochars (field: 10 Mg ha; pot: equivalent to 30 Mg ha) processed from sugar beet pulp (HSP) and beer draff (HBD) were tested against an untreated control. As a second factor, mineral nitrogen (N) fertilizer level (field: 0, 50, 100, 150 kg N ha; pot: 0, 100, 200 mg N kg soil) was varied. In both trials, hydrochars reduced initial sugar beet growth, especially when hydrochar with a high C/N ratio (38, HSP) was combined with a low N fertilizer level; high N supply partly compensated for the reduced seedling growth. Without N fertilization, no extractable N was present at the end of the pot trial in the HSP treatment, whereas in HBD even more N was extracted than in the control. This suggests remineralization of previously immobilized N when hydrochar with a low C/N ratio was applied (16, HBD). In the field, beet yield was equal at the high N fertilizer level in HSP and at all N levels in HBD treatment. Our results suggest that hydrochar can decrease plant-available N due to N immobilization. Other potential causes for the observed early growth reduction need to be studied more in detail.     

Adsorption of Cu(II) ions from aqueous solutions on biochars prepared from agricultural by-products

In this study, the adsorption of Cu(II) from aqueous solutions by agricultural by-products, such as rice husks, olive pomace and orange waste, as well as compost, was evaluated. The aim was to obtain sorbent materials (biochars) through hydrothermal treatment (300?°C) and pyrolysis (300?°C and 600?°C). The effect of adsorbent dose, pH, contact time and initial Cu(II) concentration in batch-mode experiments was investigated. The optimum Cu(II) adsorption conditions was found to occur at 5-12?g/L adsorbent dose, initial pH 5-6, and reaction time 2-4?h. Furthermore, the adsorption kinetics were best described by the pseudo-second order model for all the tested materials, while the adsorption equilibrium best fitted by the linear and Freundlich isotherms. Comparing rice husks and olive pomace, the higher adsorption capacity resulted after pyrolysis at 300?°C. With respect to the orange waste and compost, the highest adsorption capacity was observed using biochars obtained after hydrothermal treatment and pyrolysis at 300?°C.     

Non-market economic valuation of the benefits provided by temperate ecosystems at the extreme south of the Americas

The island of Navarino, Chile, located at the extreme southern end of the Americas, is one of the few regions in the world with undivided and only slightly transformed temperate forests. Currently, fundamental issues are being addressed, such as how local fuel wood demands will be met without destroying primary forests and how a sustainable tourism industry may be developed. This study aims to inform these planning processes by providing data on the economic valuation of several non-market benefits provided by the temperate ecosystems of Navarino Island that have relevance to the local population. We focus this valuation on landscape esthetics, nature access restrictions, esthetic and ethno-symbolic benefits at the species level and the existence value of non-vascular endemic species. A choice experiment was applied to a sample of local residents (n = 230). Decisions about future development strategies were influenced by landscape esthetics being threatened by progressing levels of tourist infrastructure, nature access restrictions in favor of both economic and conservationist concerns, continued visits of an ethno-culturally important hummingbird, the protection of a moss endemic to the sub-Antarctic forests and species diversity. From a non-market valuation perspective, local residents favor a low-impact tourism development scenario. Little is known about the monetary value of Chile’s temperate forests. Knowledge of the economic value of Navarino’s temperate forests facilitates the understanding of local natural resource management at the microlevel and assists in formulating conservation policies at the regional and national levels.     

Knowledge Production and Learning for Sustainable Landscapes: Forewords by the Researchers and Stakeholders

This special issue of AMBIO presents a new approach to sustainability science that goes beyond interdisciplinary research. Using coupled natural and human systems, or landscapes, as multiple case studies in Europe’s East and West knowledge production and learning toward transdisciplinary research was applied in Sweden, countries in Central and Eastern Europe, and Russia. First, the research group Forest-Landscape-Society summarizes the research program (2005–2012) behind this special issue of AMBIO and its development to participate in transdisciplinary research. Second, stakeholders at multiple levels provide their views on the new approach presented and reported.     

Dioxins (polychlorinated dibenzo-p-dioxins and polychlorinated dibenzo-furans) in traditional clay products used during pregnancy

Geophagy, the practice of consuming clay or soil, is encountered among pregnant women in Africa, Eastern Asia and Latin America, but also in Western societies. However, certain types of clay are known to contain high concentrations of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs). The aim of this study was to determine the PCDD/F contents of orally consumed clays purchased from Dutch and African markets. Congener patterns were compared with those of pooled human milk samples collected in eight African countries, to investigate a possible relationship with clay consumption. From the Dutch market thirteen clay products were examined, seven of African and six of Suriname origin. From seven African countries, twenty clay products were collected. All 33 clay products were screened with a cell-based bioassay and those showing a high response were analyzed by GC/HRMS. High PCDD/F concentrations were measured in three clay products from the Dutch market, ranging from 66 to 103 pg TEQ g^-1, whereas clay products from African countries were from 24 to 75 pg TEQ g^-1. Patterns and relatively high concentrations of PCDD/Fs in human milk samples from the Democratic Republic of the Congo and Côte d’Ivoire suggest a relationship with the consumption of contaminated clay. Frequent use of PCDD/F contaminated clay products during pregnancy may result in increased exposure of the mother and subsequently the developing fetus and new-born child. The use of these contaminated clays during pregnancy should be carefully considered or even discouraged.     

Change vector analysis to categorise land cover change processes using the tasselled cap as biophysical indicator

The continuous extraction of wood and the conversion of forest to small- and large-scale agricultural parcels is rapidly changing the land cover of the mount Cameroon region. The changes occur at varying spatial scales most often not more than 2ha for the small-scale subsistence farms and above 10ha for the extensive agricultural plantations of cocoa and palm. Given the importance of land use and land cover data in conservation planning, accurate and efficient techniques to provide up-to-date change information are required. A number of techniques for realising the detection of land cover dynamics using remotely sensed imagery have been formulated, tested and assessed with the results varying with respect to the change scenario under investigation, the information required and the imagery applied. In this study the Change Vector Analysis (CVA) technique was implemented on multitemporal multispectral Landsat data from the Thematic Mapper (TM) and Enhanced Thematic Mapper (ETM) sensors to monitor the dynamics of forest change in the mount Cameroon region. CVA was applied to multi-temporal data to compare the differences in the time-trajectory of the tasseled cap greenness and brightness for two successive time periods - 1987 and 2002. The tasseled cap was selected as biophysical indicator because it optimises the data viewing capabilities of vegetation, representing the basic types of land cover - vegetation, soil and water. Classes were created arbitrarily to predict the technique's potential in monitoring forest cover changes in the mount Cameroon region. The efficiency of the technique could not be fully assessed due to the inavailability of sufficient ground truth data. Assessment was based on the establishment of an error matrix of change versus no-change. The overall accuracy was 70%. The technique nevertheless demonstrated immense potentials in monitoring forest cover change dynamics especially when complemented with field studies.     

Berücksichtigung von Variabilität und Unsicherheit in quantitativen Risikoabschätzungen (QRA)

Using the example of residential living on a contaminated site, a probabilistic exposure assessment is performed with variability and uncertainty being modelled separately. Probability distributions are used in the exposure model in order to characterize person-related variables (e.g. body weight) only; chemical-specific parameters are being held constant. In addition, uncertainty concerning one selected variable (soil ingestion rate) was modelled. Comparing these results to conventional “worst case” estimates, we find those estimates located in the uppermost range of the probabilistic estimates. The worst case estimates tend to be highly conservative and possibly unrealistic.