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111.
A. G. Rasmussen Loft K. Nanchahal H. S. Cuckle N. J. Wald M. Hulten P. Leedham B. Nørgaard-Pedersen 《黑龙江环境通报》1990,10(7):449-459
Stored amniotic fluid samples collected in Oxford and East Birmingham as part of the Collaborative Acetylcholinesterase Study were assayed for the presence of acetylcholinesterase (AChE) using a monoclonal antibody (4F19) enzyme antigen immunoassay. These results were compared with the results of a gel AChE which had been performed earlier. A total of 5689 samples from singleton pregnancies were analysed (including 36 with anencephaly, 77 with open spina bifida and 17 with anterior abdominal wall defects). The gel test yielded detection rates of 97% for anencephaly, 99% for open spina bifida and 94% for abdominal wall defects; the false positive rate (excluding pregnancies associated with serious abnormalities, miscarriages and intrauterine deaths) was 0·24%. The monoclonal test yielded similar results; using appropriate cut-off values to allow for differences in acetylcholinesterase levels in blood stained and clear samples, a similar false-positive rate of 0·22% was associated with detection rates of 97%, 95 % and 71 % respectively for the three types of defect. Although the detection rates and the false positive rate were slightly higher for the gel test, a result that might be explained by a decrease in AChE activity caused by storage of the samples, the monoclonal test has the advantages of requiring less interpretative expertise, it can be performed on a larger number of samples a day and it is not affected by contamination with fetal calf serum. 相似文献
112.
Finn Stener Jørgensen M.D. Karin Sundberg Anne Gitte Rasmussen Loft Jørgen Arends Bent Nørgaard-Pedersen 《黑龙江环境通报》1995,15(7):621-625
Normal ranges of amniotic fluid alpha-fetoprotein (AFP) and acetylcholinesterase activity (AChE) are described for gestational weeks 11–14 using rocket gel immunoelectrophoresis for AFP quantitation and a monoclonal antibody (4F19) enzyme antigen immunoassay for AChE activity measurement. The normal ranges were established by the examination of 281 amniotic fluid samples from 281 normal pregnancies. AFP was found to increase from a median level of 14.0 MIU/1 at 11 weeks to a maximum at 13 weeks (median=18.0 MIU/l) (P<0.05), thereafter falling (not significant). No AChE test result exceeded 4.8 nkat/l. In addition, AFP and AChE values for three cases of fetal malformation, identified by the biochemical analyses of amniotic fluid, are given. These cases included two fetuses with a neural tube defect and one fetus with an abdominal wall defect. Amniocentesis was performed at 10, 11, and 14 weeks, respectively. The AFP and AChE values were all high. 相似文献
113.
L. P. D. Rasmussen 《Marine Biology》1986,92(1):59-64
The occurrence, prevalence, seasonality and possible etiology of a neoplastic disorder in natural populations of the mussel Mytilus edulis from three sites in Denmark were studied, comprising monthly sampling of 300 mussels per sample from each site during one year (October 1983 to September 1984). Neoplasia occurred at all three sites. Statistical analysis indicated that there were significant differences in the monthly prevalences of the disorder between the three sites and within each site. At two sites the prevalences were found to be seasonally related. Cytologically, the neoplasia exhibited malignant characteristics. Electron microscopy showed that the neoplastic cells were extremely pleomorphic, with large irregular nuclei. Large (approximately 200 nm diam) virus-like particles were associated with the nuclei. 相似文献
114.
N. Tommerup F. Søndergaard A. Hanauer I. Oberle J. Bang B. Barbi B. Bech K. Davies U. Froster-Iskenius K.-H. Gustavson C. B. van der Hagen A. Heiberg U. Kristoffersen M. Mikkelsen P. Miny K. B. Nielsen K. Rasmussen A. Schinzel L. Tranebjaerg T. Tønnesen J. Wahlström 《黑龙江环境通报》1991,11(8):609-619
Early prenatal diagnosis of the fragile X was attempted in 44 pregnancies, including one twin pregnancy at risk of Martin-Bell (MB) syndrome. The sex ratio was 24M:21F. The fragile site was reproducibly demonstrated in cultured chorionic villus (CV) cells in eight male and five female fetuses. Six of the male and three of the female fetuses were terminated. Simultaneous RFLP analysis provided confirmative data with flanking DNA markers in 3 of 13 analysed cases. Recombination and/or non-informativeness at available distal and/or proximal loci were found in nine cases. In one male fetus, discordance between the haplotype and cyto-genetics (fragile-X-negative) suggested the presence of a normal male transmitter, a double meiotic cross-over within the region, or a false-negative cytogenetic diagnosis. However, discordance between prenatal and post-termination/postnatal cytogenetic findings was not observed in this series. The use of excess thymidine for induction of the fragile X in cultured CV cells provided in the majority of cases a safe and rapid method for cytogenetic diagnosis, with options for early induced termination in fragile-X-positive pregnancies, for simultaneous RFLP analysis, and for subsequent second-trimester analysis of fetal blood in complicated cases. 相似文献
115.
Jens Havskov Sørensen Alix Rasmussen Thomas Ellermann Erik Lyck 《Atmospheric environment (Oxford, England : 1994)》1998,32(24):101
During the first European Tracer Experiment (ETEX) tracer gas was released from a site in Brittany, France, and subsequently observed over a range of 2000 km. Hourly measurements were taken at the National Environmental Research Institute (NERI) located at Risø, Denmark, using two measurement techniques. At this location, the observed concentration time series shows a double-peak structure occurring between two and three days after the release. By using the Danish Emergency Response Model of the Atmosphere (DERMA), which is developed at the Danish Meteorological Institute (DMI), simulations of the dispersion of the tracer gas have been performed. Using numerical weather-prediction data from the European Centre for Medium-Range Weather Forecast (ECMWF) by DERMA, the arrival time of the tracer is quite well predicted, so also is the duration of the passage of the plume, but the double-peak structure is not reproduced. However, using higher-resolution data from the DMI version of the HIgh Resolution Limited Area Model (DMI-HIRLAM), DERMA reproduces the observed structure very well. The double-peak structure is caused by the influence of a mesoscale anti-cyclonic eddy on the tracer gas plume about one day earlier. 相似文献
116.
117.
Peter F. Ffolliott D. Phillip Guertin William D. Rasmussen 《Environmental management》1988,12(6):809-814
Recurrent fire has played a dominant role in the ecology of southwestern ponderosa pine forests. To assess the benefits or losses of fire in these forests, a computer simulation model, called BURN, considers vegetation (mortality, regeneration, and production of herbaceous vegetation), wildlife (populations and habitats), and hydrology (streamflow and water quality). In the formulation of the model, graphical representations (time-trend response curves) of increases or losses (compared to an unburned control) after the occurrence of fire are converted to fixedterm annual ratios, and then annuities for the simulation components. Annuity values higher than 1.0 indicate benefits, while annuity values lower than 1.0 indicate losses. Studies in southwestern ponderosa pine forests utilized in the development of BURN are described briefly. 相似文献
118.
Culbertson JA Prins JM Grimsrud EP Rasmussen RA Khalil MA Shearer MJ 《Chemosphere》2004,55(8):1109-1119
The concentrations of CF(3)-containing compounds in archived air samples collected at Cape Meares, Oregon, from 1978 to 1997, at Point Barrow, Alaska, from 1995 to 1998, and at Palmer Station, Antarctica, from 1991 to 1997, were determined by high resolution gas chromatography and high resolution mass spectrometry. The CF(3)-containing compounds measured by this method and discussed here are: the perfluorinated compound, C(3)F(8) (FC 218); four perhalogenated compounds, CF(3)Cl (CFC 13), CF(3)CF(2)Cl (CFC 115), CF(3)CFCl(2) (CFC 114a), and CF(3)Br (Halon 1301); and three hydrofluorocompounds, CF(3)H (HFC 23), CF(3)CH(3) (HFC 143a), and CF(3)CH(2)F (HFC 134a). For four of these compounds, very few measurements have been previously reported. The atmospheric concentrations of all of the CF(3)-containing compounds continuously increased in time over the sample collection periods. From these data, the annual rates of emission into the atmosphere have been estimated. The emission rates fall into one of three distinct categories. The annual emission rates of C(3)F(8), CF(3)H, CF(3)CH(3), and CF(3)CH(2)F have continuously increased over the last two decades. That of CF(3)CFCl(2) has decreased continuously. Emission rates for CF(3)Cl, CF(3)CF(2)Cl, and CF(3)Br reached maximum levels in the late 1980s, and have been decreasing in the 1990s. The emission rates of C(3)F(8), CF(3)CH(3) and CF(3)CH(2)F were nearly zero 20 years ago but have increased rapidly during the last decade. 相似文献
119.
Pil Uthaug Rasmussen Katrine Uhrbrand Mette Damkj r Bartels Helle Neustrup Dorina Gabriela Karottki Ute B ltmann Anne Mette Madsen 《Frontiers of Environmental Science & Engineering》2021,15(3):41