Stack sampling for organic emissions

Along with increased activity in source sampling for organics, there have been many improvements in the methods of acquiring samples. Much has been learned about how best to proceed, and a number of potentially serious pitfalls have been discovered, characterized, and circumvented. Unfortunately, communication of all of this new technology has not always been effective.

This paper reviews some of the more important fundamental principles involved in stack sampling for organics, briefly describes and discusses recently developed equipment, and points out a few of the more serious pitfalls to be avoided. Extensive references are provided, many of which are often overlooked by newcomers to the field. The conclusion is reached that it is possible to consistently obtain high‐quality samples of organic materials from stationary source stacks, even though knowledge and caution are necessary.     

Traffic environment and demographic factors affecting impaired driving and crashes

IntroductionData availability has forced researchers to examine separately the role of alcohol among drivers who crashed and drivers who did not crash. Such a separation fails to account fully for the transition from impaired driving to an alcohol-related crash.MethodIn this study, we analyzed recent data to investigate how traffic-related environments, conditions, and drivers’ demographics shape the likelihood of a driver being either involved in a crash (alcohol impaired or not) or not involved in a crash (alcohol impaired or not). Our data, from a recent case–control study, included a comprehensive sampling of the drivers in nonfatal crashes and a matched set of comparison drivers in two U.S. locations. Multinomial logistic regression was applied to investigate the likelihood that a driver would crash or would not crash, either with a blood alcohol concentration (BAC) = .00 or with a BAC ≥ .05.ConclusionsTo our knowledge, this study is the first to examine how different driver characteristics and environmental factors simultaneously contribute to alcohol use by crash-involved and non-crash-involved drivers. This effort calls attention to the need for research on the simultaneous roles played by all the factors that may contribute to motor vehicle crashes.     

Network-based exploration and visualisation of ecological data

Networks – structured graphs consisting of sets of nodes connected by edges – provide a rich framework for data visualisation and exploratory analyses. Although rarely used for the visualisation of ecological data, networks are well suited to this purpose, including data that one might not normally think of as a network. We present a simple method for transforming a data matrix into network format, and show how this can be used as the basis for interactive exploratory analyses of ecological data.The method is demonstrated using a database of marine zooplankton samples acquired in the Southern Ocean. The network analyses revealed zooplankton community structures that are in good agreement with previously published results. Variations in community structure were observed to be related to the temporal and spatial pattern of sampling, as well as to physical environmental factors such as sea ice cover. The analyses also revealed a number of errors in the data, including taxon identification errors and instrument failures.The method allows the analyst to generate networks from different combinations of variables in the data set, and to examine the effects of varying parameters such as the scales of spatial, temporal, and taxonomic aggregation. This flexibility allows the analyst to rapidly gain a number of perspectives on the data and provides a powerful mechanism for exploration.     

Estimation of abundance from presence–absence maps using cluster models

A presence–absence map consists of indicators of the occurrence or nonoccurrence of a given species in each cell over a grid, without counting the number of individuals in a cell once it is known it is occupied. They are commonly used to estimate the distribution of a species, but our interest is in using these data to estimate the abundance of the species. In practice, certain types of species (in particular flora types) may be spatially clustered. For example, some plant communities will naturally group together according to similar environmental characteristics within a given area. To estimate abundance, we develop an approach based on clustered negative binomial models with unknown cluster sizes. Our approach uses working clusters of cells to construct an estimator which we show is consistent. We also introduce a new concept called super-clustering used to estimate components of the standard errors and interval estimators. A simulation study is conducted to examine the performance of the estimators and they are applied to real data.     

Effects of 4-methylbenzylidene camphor (4-MBC) on neuronal and muscular development in zebrafish (<Emphasis Type="Italic">Danio rerio</Emphasis>) embryos

The negative effects of overexposure to ultraviolet (UV) radiation in humans, including sunburn and light-induced cellular injury, are of increasing public concern. 4-Methylbenzylidene camphor (4-MBC), an organic chemical UV filter, is an active ingredient in sunscreen products. To date, little information is available about its neurotoxicity during early vertebrate development. Zebrafish embryos were exposed to various concentrations of 4-MBC in embryo medium for 3 days. In this study, a high concentration of 4-MBC, which is not being expected at the current environmental concentrations in the environment, was used for the purpose of phenotypic screening. Embryos exposed to 15 μM of 4-MBC displayed abnormal axial curvature and exhibited impaired motility. Exposure effects were found to be greatest during the segmentation period, when somite formation and innervation occur. Immunostaining of the muscle and axon markers F59, znp1, and zn5 revealed that 4-MBC exposure leads to a disorganized pattern of slow muscle fibers and axon pathfinding errors during the innervation of both primary and secondary motor neurons. Our results also showed reduction in AChE activity upon 4-MBC exposure both in vivo in the embryos (15 μM) and in vitro in mammalian Neuro-2A cells (0.1 μM), providing a possible mechanism for 4-MBC-induced muscular and neuronal defects. Taken together, our results have shown that 4-MBC is a teratogen and influences muscular and neuronal development, which may result in developmental defects.     

Rising trends of dissolved organic matter in drinking-water reservoirs as a result of recovery from acidification in the Ore Mts., Czech Republic

The concentration of chemical oxygen demand (COD), a common proxy for dissolved organic matter (DOM), was measured at seven drinking-water reservoirs and four streams between 1969 and 2006. Nine of them showed significant DOM increases (median COD change +0.08 mg L⁻¹ yr⁻¹). Several potential drivers of these trends were considered, including air temperature, rainfall, land-use and water sulfate concentration. Temperature and precipitation influenced inter-annual variations, but not long-term trends. The long-term DOM increase was significantly associated with declines of acidic deposition, especially sulfur deposition. Surface water sulfate concentrations decreased from a median of 62 mg L⁻¹-27 mg L⁻¹ since 1980. The magnitude of DOM increase was positively correlated with average DOM concentration (R² = 0.79, p < 0.001). Simultaneously, DOM concentration was positively correlated with the proportion of Histosols within the catchments (R² = 0.79, p < 0.001). A focus on the direct removal of DOM by water treatment procedures rather than catchment remediation is needed.     

Variation in Pesticide Tolerance of Tadpoles among and within Species of Ranidae and Patterns of Amphibian Decline

Abstract: There is significant variation among and within amphibian species with respect to reports of population decline; declining species are often found in environments that are physiograpically similar to environments where the same species is thriving. Because variability exists among organisms in their sensitivity to environmental stressors, it is important to determine the degree of this variation when undertaking conservation efforts. We conducted both lethal (time-to-death) and sublethal (activity change) assays to determine the degree of variation in the sensitivity of tadpoles to a pesticide, carbaryl, at three hierarchical levels: among ranid species, among several populations of a single ranid species (   Rana sphenocephala ), and within populations of R. sphenocephala . We observed significant variation in time to death among the nine ranid species and among the 10 R. sphenocephala populations we tested. Four out of eight R. sphenocephala populations exhibited significantly different times to death among families. The magnitude of the activity change in response to exposure to sublethal carbaryl levels was significantly different among species and within R. sphenocephala populations. Chemical contamination, at lethal or sublethal levels, can alter natural regulatory processes such as juvenile recruitment in amphibian populations and should be considered a contributing cause of declines in amphibian populations.     

Novel approach to monitoring of the soil biological quality

In this study, a new approach to interpretation of results of the simple microbial biomass and respiration measurements in the soil microbiology is proposed. The principle is based on eight basal and derived microbial parameters, which are standardized and then plotted into sunray plots. The output is visual presentation of one plot for each soil, which makes possible the relative comparison and evaluation of soils in the monitored set. Problems of soil microbiology, such as the lack of benchmarking and reference values, can be avoided by using the proposed method. We found that eight parameters provide enough information for evaluation of the status of the soil microorganisms and, thus, for evaluation of the soil biological quality. The usage of rare parameters (potential respiration PR, ratio of potential and basal respiration PR/BR, biomass-specific potential respiration PR/C(bio), available organic carbon C(ext), and biomass-specific available organic carbon C(ext)/C(bio)) can be recommended, besides classical and well-known parameters (microbial biomass C(bio), basal respiration BR, metabolic coefficient qCO(2)). The combination of basal parameters and derived coefficients can also extend our knowledge about the condition of the soil microorganisms. In monitoring the case studies presented, we observed that soils evaluated to possess good biological quality displayed generally higher values of organic carbon, total nitrogen, clay, and cation exchange capacity. The soils of good biological quality can display higher levels of contaminants. This is probably related with the higher content of organic carbon and clay in these soils.     

Root respiration and oxygen flux in salt marsh grasses from different elevational zones

Plants growing in waterlogged environments are subjected to low oxygen levels around submerged tissues. While internal oxygen transport has been postulated as an important factor governing flooding tolerance, respiration rates and abilities to take up oxygen under hypoxic conditions have been largely ignored in plant studies. In this study, physiological characteristics related to internal oxygen transport, respiration, and oxygen affinity were studied in low intertidal marsh species (Spartina alterniflora and S. anglica) and middle to high intertidal species (S. densiflora, S. patens, S. foliosa, a S. alterniflora × S. foliosa hybrid, S. spartinae, and Distichlis spicata). These marsh plants were compared to the inland species S. pectinata and the crop species rice (Oryza sativa), corn (Zea mays), and oat (Avena sativa). Plants were grown in a greenhouse under simulated estuarine conditions. The low marsh species S. anglica was found to transport oxygen internally at rates up to 2.2 μmol O₂ g fresh root weight⁻¹ h⁻¹. In contrast, marsh species from higher zones and crop species were found to transport significantly less oxygen internally, although rice plants were able to transport 1.4 μmol g⁻¹ h⁻¹. Under hypoxic conditions, low marsh species were better able to remove dissolved oxygen from the medium compared to higher marsh species and crops. The oxygen concentration at which respiration rates declined due to limited oxygen (P _crit) was significantly lower in low marsh species compared to inland and crop species; P _crit ranged from <4 μM O₂ in the low marsh species S. anglica up to 20 μM in the inland species corn. Flooding-sensitive crop species had significantly higher aerobic respiration rates compared to flooding-tolerant species in this study. Crop species took up 3.6–6.7 μmol O₂ g⁻¹ h⁻¹ while all but one marsh species took up <3.5 μmol O₂ g⁻¹ h⁻¹. We conclude that oxygen transport, aerobic demand, and oxygen affinity all play important and interrelated roles in flood tolerance and salt marsh zonation.