Effect of deuterium-depleted water on reproduction of rainbow trout

During the artificial reproduction of salmonides, the fecundity can be increased either by improving the viability of spermatozoa, or by extending the time period during which a roe micropile remains open, thus allowing its fecundation. Practically, this can be achieved by the use of some fertilising techniques suitable for fish species. Here, we show that the reproduction of rainbow trout in a 1:1 solution of deuterium-depleted water and distilled water led to a significant increase in survival of roes during their embryonic development. Moreover, the addition of glucose and fructose into the deuterium-depleted fertilising solutions led to a further increase in roe survival during embryonic development. The increase in survival is mainly explained by an increase in the motility of spermatozoa.     

Toxicity of uranium mine receiving waters to early life stage fathead minnows (Pimephales promelas) in the laboratory.

Elevated concentrations of arsenic, nickel, and molybdenum in aquatic systems around northern Saskatchewan uranium mines are an environmental concern. Early life stage fathead minnows were used to assess toxicity from several aquatic systems near the Key Lake and Rabbit Lake uranium operations. Hatching success of fish embryos exposed to waters receiving contaminants associated with uranium ore milling was reduced by 32-61% relative to controls. Mortality differed in two lakes receiving mill effluents because of opposing factors influencing metal toxicity (i.e. low pH and high hardness). In one mill receiving water (Fox Lake), larval mortality was 0%, whereas mortality was 85% in water collected from a downstream location (Unknown Lake). Fish embryos exposed to open-pit dewatering effluent receiving waters, or water from a flooded open pit (i.e. pit waters), hatched 26-39% earlier than those exposed to reference or control water. The combination of low water hardness and elevated nickel concentrations in pit waters contributed to the early hatching. Egg hatchability and hatching time were more sensitive indicators of toxicity than 'standard' endpoints, like larval mortality and growth. Current regulatory emphasis on single contaminants and standard toxicological endpoints should be re-evaluated in light of the complex interaction among confounding variables such as pH, hardness. conductivity, and multi-metal mixtures.     

Pentachlorotoluene and pentabromotoluene: results of a subacute and a subchronic toxicity study in the rat

Pentachlorotoluene (PCT) and pentabromotoluene (PBT) are environmental contaminants detected in the Great Lakes ecosystem. In view of the paucity of toxicity data and the potential for human exposure, a subacute (28 day) and a subchronic (91 day) study were conducted in the rat. In the subacute study, groups of 10 male and 10 female rats were fed the diet containing PCT or PBT at 0, 0.5, 5.0, 50 or 500 ppm for 28-days. In the subchronic study, the group size was increased to 15, the dose levels were 0, 0.05, 0.5, 5.0, 50 and 500 ppm in the diet and the exposure period was 91 days. Growth rate and food consumption were not affected by exposure to either chemical in the subacute and subchronic study. Clinical observations revealed no abnormalities. Decreased hemoglobin was observed in female rats fed 5.0 ppm PCT and higher levels in the subacute (28 day) study. In the same study the hematocrit value and erythrocyte numbers of females fed 5.0 or 500 ppm PCT diets were significantly lower than the control. In both subacute and subchronic studies mild dose-dependent histopathological changes were observed in the thyroid, liver and kidney of rats fed PCT and PBT diets. In general male rats were more susceptible than females to the treatment of PCT and PBT. Based on these data, it was concluded that the no observable adverse effect level for PCT was 50 ppm in the diet (3.5 mg/kg b.w./day) and that of PBT was 5.0 ppm (0.35 mg/kg b.w./day).     

The Spatiotemporal Pattern of Fires in Northern Taiga Larch Forests of Central Siberia

The periodicity of fires in larch forests of Evenkia and their relationship with landscape elements have been studied. Cross-sections with “burns” in them caused by past fires have been analyzed in 72 test plots; the fire chronology encompassed the period from the 15th to the 20th century. The between-fire intervals (BFIs) have been calculated by two methods: (I) on the basis of burns alone and (II) on the basis of burns and the start of growth of the new generation of larch after the earliest fire. The BFI depends on local orographic features; it is 86 ± 11 (105 ± 12), 61 ± 8 (73 ± 8), 139 ± 17 (138 ± 18), and 68 ± 14 (70 ± 13) years for northeastern slopes, southwestern slopes, bogs, and flatlands, respectively. The mean BFIs calculated by methods I and II are 82 ± 7 and 95 ± 7 years, respectively. The permafrost horizon rises at a mean rate of 0.3 cm per year after a forest fire. It has been shown that the number of fires regularly peaks at periods of 36 and 82 years. There is also a temporal trend in fire frequency: the mean BFI was approximately 100 years in the 19th century and 65 years in the 20th century.     

Effects of two organophosphorus insecticides on some biochemical constituents in the nervous system and liver of rabbits

Male Baladi rabbits were acutely and sub-chronically intoxicated with cyanofenphos and profenophos. The levels of cholesterol, triglycerides, B-lipoproteins and total proteins were determined in the serum, brain, spinal cord and sciatic nerve of rabbits. Moreover, the activities of alkaline phosphatase, acid phosphatase and glutamic-pyruvic transaminase were determined in the liver of the animals. The whole studies revealed that the biochemical constituents were highly affected by the tested insecticides. Also, the liver function suffered from adverse effects of the tested insecticides.     

Coat color polymorphism in populations of the northern mole vole in the Ural Region

Collections made in the course of long-term field studies on ecology of the northern mole vole Ellobius talpinus Pall. in the Ural Region and neighboring areas (more than 2000 individuals from 24 points of the species range) were used to analyze geographic variation in its coat color (color morphs). On the basis of long-term observations (1985–1999) on marked animals from a polymorphic population (Kurtamyshskii raion, Kurgan oblast), the life spans of males and females and the dependence of life span on population density and structure were estimated in animals of different color morphs. Each color morph of E. talpinus was shown to have specific features of the seasonal dynamics of age structure and migrations.     

RESEARCH: Shrub Propagation Techniques for Biological Control of Invading Tree Species

Cornus stolonifera, Salix petiolaris, and Spiraea alba clones already located within the corridor of an electrical power line. To establish the efficiency of treatments, we examined the statistical differences of growth traits between species and treatments. An analysis of the effects of layering shows, after the first growth season, differences for all growth traits in only one species, Spiraea alba. After the second growth season, we observed the development of new aerial stems. Layering favors horizontal expansion of shrubs over height development. The third year after treatment, the effect of layering is reduced except for Cornus stolonifera, which continuously increases, as shown by the significant progression of the clone issued from the layer even five years after treatments. With the cutting back technique, we expected a distinct vertical growth of the shrubs at the expense of increasing the crown diameter. This technique would be best associated with the rejuvenation of clones, followed by a layering of new shoots to allow a horizontal expansion of the shrubs. Therefore, the formation of a dense shrub community by layering should be considered a valuable approach for the biological control of undesirable trees in powerline rights-of-way.