Diffuse source apportionment of the Po river eutrophying load to the Adriatic sea: assessment of Lombardy contribution to Po river nutrient load apportionment by means of an integrated modelling approach

The source apportionment of the annual nutrient load carried by the Po river to the Adriatic sea has been studied.

An integrated modelling approach was applied to the Lombardy plain area, which covers about 34% of the Po river watershed area and accounts for about 50% of the point sources’ loads carried by the river. To extract all the information available from direct instream measurements, two different modelling tools were alternatively used. The source apportionment was investigated considering both dry and wet weather scenarios. In order to quantify the apportionment in dry-weather conditions, the Lombardy portion of the Po river basin was modelled by using the US-EPA QUAL2E model. Such a simulation allowed to assess a significant contribution (about 50% of the total dry-weather load) of a not rain-driven diffuse pollution component (i.e. groundwater, springs, lake emissaries). Moreover, to estimate the rain-driven surface runoff contribution to the instream total load, the Lombardy plain area was also modelled by means of the US-DA SWAT model. SWAT results indicate a runoff contribution to the Po river instream total load of about 10 000 t N yr⁻¹ and 1300 t P yr⁻¹ (i.e. approximately the 10–20% of the total annual Lombardy nutrient load). At the event scale (i.e. the single rainstorm event) the runoff contribution may rise up to 30–80% of the total instream load. Finally, the total annual nitrogen load at the Po basin closure was estimated for the period 1985–2001. Out of a total annual load of 140 000 t N yr⁻¹, Lombardy accounts for 43% (point plus diffuse sources). The rain-driven diffuse sources constitute the 20% of the overall total load, the point sources account for 40%, whereas the remaining 40% is mainly constituted by “dry-weather diffuse sources” (i.e. groundwater, springs, lake emissaries).     

Efficiency of physicochemical and biological treatments of vinasse and their influence on indigenous microbiota for disposal into the environment

Molasses-based distilleries are one of the most polluting industries generating large volumes of high strength wastewater called vinasse. Different processes covering anaerobic, aerobic as well as physicochemical methods have been employed to treat this effluent. This study evaluated the microbial communities present in the vinasse during different stages of its treatment by traditional and molecular methods. The analysis of the efficiency of each treatment was performed by physicochemical parameters and toxicity analysis. The treatment of vinasse was performed in the following steps: high flow fermentation; filtration; chemical flakes; low-flow fermentation; filtration; and neutralization. The physicochemical analysis in different stages of the vinasse treatment demonstrated that phases of treatment influenced the performance of the evaluated parameters. Among the 37 parameters, 9 were within the limits established by the Commission for Environmental Policy of Minas Gerais, Brazil (COPAM), especially BOD (96.7% of pollution reduction), suspended solids (99.9%), pH, copper (88%), iron (92.9%), and manganese (88%). Some parameters, even after treatment, did not fit the maximum allowed by legislation. The microbial population decreased reaching 3 log CFU/ml present in the steps of the flakes chemical and disinfection treatment of vinasse. Lactobacillus brevis and Pichia kudriavzevii were present in all stages of the treatments, showing that these microorganisms were resistant and demonstrated that they might be important in the treatment of vinasse. The vinasse showed a significant reduction of pollution load after the disinfection treatment however still should not be discarded into water bodies because the high values of tannins and sediment solids, but suggest the use of the effluent in the cooling coil during the distillation process of the beverage.     

An assessment of coastal land-use and land-cover change from 1974–2008 in the vicinity of Mobile Bay,Alabama

The purpose of this research is to quantify and assess geospatial land-use and land-cover (LULC) changes in the coastal counties of Mobile and Baldwin, Alabama using nine Landsat images from 1974–2008. A study-specific classification scheme was devised comprising upland herbaceous, upland forest, non-woody and woody wetlands, open water, and urban categories. Upland forest was the most dominant terrestrial cover type. Wetlands averaged 17% and urban averaged 7%. A majority of the urban expansion occurred between 1974 and 1979 (26%). Thirty-four percent of the 2008 urban areas were upland forest in 1974. Watershed-scale analysis of Three Mile Creek and D’Olive Bay highlights the temporal and spatial differences of urbanization for watersheds found within the same region. This study is a Gulf of Mexico Alliance (GOMA) Application Pilot project that uses NASA data products to benefit coastal environmental managers and community members. Results have led to increased effectiveness of coastal conservation decision-making, increased understanding of post-hurricane LULC change, continued research on habitat change impacts, and contributed to timely conservation planning efforts. This study has benefited the development of watershed management plans by the Mobile Bay National Estuary Program, which is especially important given projected climate change.     

Isolation of single circulating trophoblasts from maternal circulation for noninvasive fetal copy number variant profiling

Objective

To develop a multi-step workflow for the isolation of circulating extravillous trophoblasts (cEVTs) by describing the key steps enabling a semi-automated process, including a proprietary algorithm for fetal cell origin genetic confirmation and copy number variant (CNV) detection.

Methods

Determination of the limit of detection (LoD) for submicroscopic CNV was performed by serial experiments with genomic DNA and single cells from Coriell cell line biobank with known imbalances of different sizes. A pregnancy population of 372 women was prospectively enrolled and blindly analyzed to evaluate the current workflow.

Results

An LoD of 800 Kb was demonstrated with Coriell cell lines. This level of resolution was confirmed in the clinical cohort with the identification of a pathogenic CNV of 800 Kb, also detected by chromosomal microarray. The mean number of recovered cEVTs was 3.5 cells per sample with a significant reverse linear trend between gestational age and cEVT recovery rate and number of recovered cEVTs. In twin pregnanices, evaluation of zygosity, fetal sex and copy number profiling was performed in each individual cell.

Conclusion

Our semi-automated methodology for the isolation and single-cell analysis of cEVTS supports the feasibility of a cell-based noninvasive prenatal test for fetal genomic profiling.     

An alternative way to determine the size distribution of airborne particulate matter

We developed and tested a methodology to extract both the size-segregated source apportionment of atmospheric aerosol and the size distribution of each detected element. The experiment is based on the parallel use of a standard low-volume sampler to collect Particulate Matter (PM) and an Optical Particle Counter (OPC). The approach is complementary to size-segregated PM sampling, and it was tested versus a 12-stage cascade impactor. Samples were collected inside the urban area of Genoa (Italy) and their elemental composition was measured by Energy Dispersive-X Ray Fluorescence (ED-XRF). Positive Matrix Factorization (PMF) was applied to time series of elemental concentrations to identify major PM sources, and both PM mass concentration and size-segregated particle number concentration were apportioned. Source profiles and temporal trends extracted by PMF were analyzed together with the OPC data to obtain the size distribution for several elements. The new methodology proved to be reliable for the PM apportionment as well as in providing the elemental concentrations in PM10, PM2.5, and PM1 (PM with aerodynamic diameter, D_ae < 10, 2.5, and 1 μm, respectively). The elemental size distributions are in good agreement with those obtained by the cascade impactor for several elements but some discrepancies, in particular for traffic emissions, are stressed and discussed in the text. The new methodology has two main advantages: it only requires standard semi-automatic sampling equipment and compositional analysis and it provides size-segregated information averaged over quite long periods (typically several months). This is particularly important since campaigns with cascade impactors are generally laborious and thus limited to short periods.     

PCDD/F mass balance in the flue gas cleaning units of a MSW incineration plant

With the main purpose of evaluating PCDD/F presence and the corresponding mass balance over the emissions control system, an extensive study was performed on a municipal solid waste full scale incinerator equipped with a best available technology flue gas treatment line. Present paper reports the main results obtained, with particular reference to the PCDD/F concentration profiles and mass balances derived for every process unit of the flue gas control system. Total release evaluated for the plant is also outlined compared with data on PCDD/F content of raw waste and with reference values included in most recent guidelines.     

The Triple Bottom Line for Sustainable Community Development

Rural communities in Australia are confronting a period of unprecedented change. They face declining commodity prices, the withdrawal of both public and private sector services, such as banks and hospitals, and the closure of small businesses. Their populations are in decline, with young people leaving for the larger regional centres. Many also face a declining resource base as soils become saline and water resources are sold to areas with higher unit returns. This paper presents a framework for sustainable community development based on emerging ideas of performance auditing and the development of progress indicators. The triple bottom line community audit approach is recast for the community level, measuring performance on improved community wellbeing, reduced environmental impact and increased economic vitality. Rural communities need to redefine their future. A sustainability focus offers a way forward. Las comunidades rurales en Australia estan afrontando un periodo de cambio sin precedente. Enfrentan disminución en los precios de los productos, retiro de servicios de ambos sectores, publico y privado, como bancos y hospitales y el cierre de pequeños negocios. Su población está disminuyendo, la gente joven emigra hacia centros regionales mas grandes. Muchos tambien enfrentan un deterioro en los recursos base ya que los suelos se tornan salinos, y los recursos de agua son vendidos a áreas con retorno de unidad mas alto. Este documento presenta una estructura para el desarrollo comunitario sostenible basado en el surgimiento de ideas de la revisión del desempeño y del desarrollo de indicadores de progreso. El enfoque de auditoria del triple resultado final esta rehecho para el nivel de la comunidad, midiendo el desempeño en el bienestar de la comunidad mejorada, el impacto ambiental reducido, y la vitalidad económica incrementada. Las comunidades rurales necesitan redefinir su futuro. Un enfoque sostenible ofrece una vía hacia adelante.     

90Sr, 137Cs and (239,240)Pu concentration surface water time series in the Pacific and Indian Oceans--WOMARS results

Under an IAEA's Co-ordinated Research Project "Worldwide Marine Radioactivity Studies (WOMARS)" 90Sr, 137Cs and (239,240)Pu concentration surface water time series in the Pacific and Indian Oceans have been investigated. The Pacific and Indian Oceans were divided into 17 latitudinal boxes according to ocean circulation, global fallout patterns and the location of nuclear weapons test sites. The present levels and time trends in radionuclide concentrations in surface water for each box were studied and the corresponding effective half-lives were estimated. For the year 2000, the estimated average 90Sr, 137Cs and (239,240)Pu concentrations in surface waters of the Pacific and Indian Oceans varied from 0.1 to 1.5 mBq/L, 0.1 to 2.8 mBq/L, and 0.1 to 5.2 microBq/L, respectively. The mean effective half-lives for 90Sr and 137Cs in surface water were 12+/-1 years for the North, 20+/-1 years for the South and 21+/-2 years for the Equatorial Pacific. For (239,240)Pu the corresponding mean effective half-lives were 7+/-1 years for the North, 12+/-4 years for the South and 10+/-2 years for the Equatorial Pacific. For the Indian Ocean the mean effective half-lives of 137Cs and (239,240)Pu were 21+/-2 years and 9+/-1 years, respectively. There is evidence that fallout removal rates before 1970 were faster than those observed during recent decades. The estimated surface water concentrations of 90Sr, 137Cs and (239,240)Pu in latitudinal belts of the Pacific and Indian Oceans for the year 2000 may be used as the average levels so that any new contribution from nuclear facilities, nuclear weapons test sites, radioactive waste dumping sites and from possible nuclear accidents can be identified.