HPLC determination of chlorate metabolism in bovine ruminal fluid

Salmonella and Escherichia coli are two bacteria that are important causes of human and animal disease worldwide. Chlorate is converted in the cell to chlorite, which is lethal to these bacteria. An HPLC procedure was developed for the rapid analysis of chlorate (ClO₃ ^?), nitrate (NO₃ ^?), and nitrite (NO₂ ^?) ions in bovine ruminal fluid samples. Standard curves for chlorite, nitrite, nitrate, and chlorate were well defined linear curves with R² values of 0.99846, 0.99106, 0.99854, and 0.99138, respectively. Concentrations of chlorite could not be accurately determined in bovine ruminal fluid because chlorite reacts with or binds a component(s) or is reduced to chloride in bovine ruminal fluid resulting in low chlorite measurements. A standard curve ranging from 25 to 150 ppm ClO₃ ^? ion was used to measure chlorate fortified into ruminal fluid. The concentration of chlorate was more rapidly lowered (P < 0.01) under anaerobic compared to aerobic incubation conditions. Chlorate alone or chlorate supplemented with the reductants sodium lactate or glycerol were bactericidal in anaerobic incubations. In anaerobic culture, the addition of sodium formate to chlorate-fortified ruminal fluid appeared to decrease chlorate concentrations; however, formate also appeared to moderate the bactericidal effect of chlorate against E. coli. Addition of the reductants, glycerol or lactate, to chlorate-fortified ruminal fluid did not increase the killing of E. coli at 24 h, but may be useful when the reducing equivalents are limiting as in waste holding reservoirs or composting systems required for intense animal production.     

Trends in atmospheric reactive nitrogen for the Eastern United States

Reactive nitrogen can travel far from emission sources and impact sensitive ecosystems. From 2002 to 2006, policy actions have led to decreases in NO(x) emissions from power plants and motor vehicles. In this study, atmospheric chemical transport modeling demonstrates that these emissions reductions have led to a downward trend in ambient measurements of transported reactive nitrogen, especially atmospheric concentrations and wet deposition of nitrate. The trend in reduced nitrogen, namely ammonium, is ambiguous. As reduced nitrogen becomes a larger fraction of the reactive nitrogen budget, wide-spread NH(3) measurements and improved NH(3) emissions assessments are a critical need.     

Fish Migration, Dams, and Loss of Ecosystem Services in the Mekong Basin

The past decade has seen increased international recognition of the importance of the services provided by natural ecosystems. It is unclear however whether such international awareness will lead to improved environmental management in many regions. We explore this issue by examining the specific case of fish migration and dams on the Mekong river. We determine that dams on the Mekong mainstem and major tributaries will have a major impact on the basin’s fisheries and the people who depend upon them for food and income. We find no evidence that current moves towards dam construction will stop, and consider two scenarios for the future of the fisheries and other ecosystems of the basin. We conclude that major investment is required in innovative technology to reduce the loss of ecosystem services, and alternative livelihood strategies to cope with the losses that do occur.     

Effects of Heating Season on Residential Indoor and Outdoor Polycyclic Aromatic Hydrocarbons, Black Carbon, and Particulate Matter in an Urban Birth Cohort

Exposure to air pollutants has been associated with adverse health effects. However, analyses of the effects of season and ambient parameters such as ozone have not been fully conducted. Residential indoor and outdoor air levels of polycyclic aromatic hydrocarbons (PAH), black carbon (measured as absorption coefficient [Abs]), and fine particulate matter <2.5 μm (PM)(2.5) were measured over two-weeks in a cohort of 5-6 year old children (n=334) living in New York City's Northern Manhattan and the Bronx between October 2005 and April 2010. The objectives were to: 1) characterize seasonal changes in indoor and outdoor levels and indoor/outdoor (I/O) ratios of PAH (gas + particulate phase; dichotomized into Σ(8)PAH(semivolatile) (MW 178-206), and Σ(8)PAH(nonvolatile) (MW 228-278)), Abs, and PM(2.5); and 2) assess the relationship between PAH and ozone. Results showed that heating compared to nonheating season was associated with greater Σ(8)PAH(nonvolatile) (p<0.001) and Abs (p<0.05), and lower levels of Σ(8)PAH(semivolatile) (p<0.001). In addition, the heating season was associated with lower I/O ratios of Σ(8)PAH(nonvolatile) and higher I/O ratios of Σ(8)PAH(semivolatile) (p<0.001) compared to the nonheating season. In outdoor air, Σ(8)PAH(nonvolatile) was correlated negatively with community-wide ozone concentration (p<0.001). Seasonal changes in emission sources, air exchanges, meteorological conditions and photochemical/chemical degradation reactions are discussed in relationship to the observed seasonal trends.     

Overview of deaths associated with natural events, United States, 1979-2004.

Analysis of the National Center for Health Statistics' Compressed Mortality File showed that between 1979 and 2004, natural events caused 21,491 deaths in the United States. During this 26-year period, there were 10,827 cold-related deaths and 5,279 heat-related deaths. Extreme cold or heat accounted for 75 per cent of the total number of deaths attributed to natural events--more than all of deaths resulting from lightning, storms and foods, and earth movements, such as earthquakes and landslides. Cold-related death rates were highest in the states of Alaska, Montana, New Mexico, and South Dakota, while heat-related deaths were highest in the states of Arizona, Missouri, and Arkansas. These deaths occurred more often among the elderly and black men. Other deaths were attributed to lightning (1,906), storms and foods (2,741), and earth movements (738). Most deaths associated with natural events are preventable and society can take action to decrease the morbidity and mortality connected with them.     

Monitoring brownfield housing development: strengths and weaknesses of indicator based monitoring in the English planning system

In England quantified targets for the reuse of brownfields and of existing buildings place particular requirements on the planning system as part of the overall strategy to make effective use of natural resources. Achieving these targets is not free of obstacles and potential conflicts. Therefore monitoring of policy implementation is a necessity in the planning system. In addition challenging targets for increased housing provision have been introduced. In view of this the dichotomy of adequate housing-land supply without compromising environmental quality must be addressed. As a consequence monitoring will play an increasingly important role. This paper explores how these challenges can be met. It draws upon practical experience across all planning tiers to highlight the potential and limitations of indicator based monitoring.     

Sectoral sustainability and sustainability assessment methodologies: A review of methodology in light of collaboration with the UK oil and gas sector

This paper discusses methodologies that have been employed to give insights into business performance in relation to sustainable development, both at a sectoral and corporate level. From consideration of different sectoral sustainable development methodologies, some key issues and good practice related to their derivation are elaborated. These issues are then further illustrated by a detailed comparison between three sustainable development methodologies that have been used in the UK offshore oil and gas industry. The methodologies chiefly differ in terms of how they address the problem of comparing different impacts across potentially non-commensurable dimensions, one proceeding through explicit monetary valuation, another through implicit valuation of different options and another through presentation of an unaggregated framework of indicators. The paper concludes by identifying the different situations in which use of these different methodologies is appropriate.     

Low back pain among residential carpenters: ergonomic evaluation using OWAS and 2D compression estimation.

Occupational low back pain (LBP) remains a leading safety and health challenge. This cross-sectional investigation measured the prevalence of LBP in residential carpenters and investigated ergonomic risk factors. Ninety-four carpenters were investigated for LBP presence and associated risk factors. Ten representative job-tasks were evaluated using the Ovako Working Posture Analysis System (OWAS) and ErgoMaster 2D software to measure elements of posture, stress, and risk. Job-tasks were found to differ significantly for total lumbar compression and shear at peak loading (p < .001), ranging from 2 956 to 8 606 N and 802 to 1 974 N respectively. OWAS indicated that slight risk for injury was found in 10 job-tasks while distinct risk was found in 7 of the 10 job-tasks. Seven of the 10 job-tasks exceeded the National Institute for Occupational Safety and Health (NIOSH) action limit of 3 400 N for low back loading. The point prevalence for LBP was 14% while the annual prevalence was 38%.     

Book reviews

French Land Use Planning in the Fifth Republic: Real or imagined decentralisation?

Irene B. Wilson, Nijmeegse Planologische Cahiers No. 27, Geografisch en Planologisch Instituut, Katholieke Universiteit Nijmegen, Netherlands, 1988. 228pp. ISBN 90–70219–23–9.

Environmental and Resource Economics: an Introduction

Common, M. (1988). Longman i‐xiv 1–319.

The Greening of Aid: Sustainable Livelihoods in Practice.

C. Conroy & M. Litvinoff (Eds.) Earthscan Publications Ltd, London, 1989, pp.293, £8.95 (pb).

Private Supply of Public Services: Evaluation of Real Estate Exactions, Linkage, and Alternative Land Policies.

Rachelle Alterman (Ed), New York University Press, 1988. 273pp, ISBN 0–8147–0589–8.

City Politics

Hegemonic Projects and Discourse, by Maarten A. Hajer, published by Avebury Press, the Gower Publishing Company Ltd 1989, 140 pp.

The Genius Of The Place

The English Landscape Garden 1620–1820

John Dixon Hunt and Petér Willis. Price £12.50 (Paperback) & £26.95 (Cloth). M.I.T. Press.

Vacationscape

Designing Tourist Regions — 1988 2nd Ed.

Clare A. Gunn, New York, Van Nostrand Reinhold, $34.95 (cloth)

Loft Living — Culture and Capital in Urban Change

Sharon Zukin. Published, British Edition ‘Radius (Century Hudson) London, 1988 Price £8.95.

First Pub. U.S. John Hopkins University Press, 1982. Note: The British Edition has an added Chapter ’More Market Forces’.

William Kent Landscape Garden Designer

John Dixon Hunt. A. Zwemmer Ltd London. 1987, 176pp.

The Pattern of Landscape

Sylvia Crowe and Mary Mitchell (1988). Packard Publishing Ltd, Chichester. 127pp, 161 photographs. Price £30. ISBN 185341 020.

International Environmental Diplomacy

Edited by J. E. Carroll