全文获取类型
收费全文 | 20339篇 |
免费 | 182篇 |
国内免费 | 201篇 |
专业分类
安全科学 | 534篇 |
废物处理 | 986篇 |
环保管理 | 2419篇 |
综合类 | 2670篇 |
基础理论 | 5284篇 |
环境理论 | 4篇 |
污染及防治 | 5598篇 |
评价与监测 | 1651篇 |
社会与环境 | 1453篇 |
灾害及防治 | 123篇 |
出版年
2023年 | 92篇 |
2022年 | 215篇 |
2021年 | 217篇 |
2020年 | 139篇 |
2019年 | 177篇 |
2018年 | 326篇 |
2017年 | 320篇 |
2016年 | 525篇 |
2015年 | 362篇 |
2014年 | 573篇 |
2013年 | 1745篇 |
2012年 | 672篇 |
2011年 | 859篇 |
2010年 | 803篇 |
2009年 | 785篇 |
2008年 | 887篇 |
2007年 | 976篇 |
2006年 | 870篇 |
2005年 | 716篇 |
2004年 | 723篇 |
2003年 | 704篇 |
2002年 | 670篇 |
2001年 | 911篇 |
2000年 | 631篇 |
1999年 | 393篇 |
1998年 | 274篇 |
1997年 | 246篇 |
1996年 | 291篇 |
1995年 | 270篇 |
1994年 | 250篇 |
1993年 | 233篇 |
1992年 | 240篇 |
1991年 | 208篇 |
1990年 | 214篇 |
1989年 | 219篇 |
1988年 | 197篇 |
1987年 | 158篇 |
1986年 | 126篇 |
1985年 | 138篇 |
1984年 | 169篇 |
1983年 | 153篇 |
1982年 | 193篇 |
1981年 | 134篇 |
1980年 | 119篇 |
1979年 | 152篇 |
1978年 | 118篇 |
1977年 | 107篇 |
1976年 | 100篇 |
1975年 | 83篇 |
1974年 | 88篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
251.
We report the first prenatal diagnosis of an affected fetus with Chediak-Higashi syndrome (CHS). Diagnosis was accomplished via fetal blood sampling at 17 menstrual weeks and was confirmed after birth. Retrospective measurement of the largest acid phosphatase-positive lysosomes in cultured amniotic fluid cells and chorionic villus cells showed that in CHS these lysosomes are significantly larger than those in normal cells. This method may be used for prenatal diagnosis of CHS by amniocentesis and chorionic villus sampling (CVS). 相似文献
252.
Diana W. Bianchi MD Gretchen K. Zickwolf Melissa C. Yih Alan F. Flint Ossie H. Geifman Marlena S. Erikson John M. Williams 《黑龙江环境通报》1993,13(4):293-300
Fetal nucleated erythrocytes (NRBC) in maternal blood are a non-invasive source of fetal DNA for prenatal genetic screening. We compared the effectiveness of three monoclonal antibodies for the separation of fetal cells from maternal blood by flow sorting. Mononuclear blood cells from 49 healthy pregnant women were incubated with antibody to CD 71, CD 36, and/or glycophorin A (GPA), employed singly or in combination with each other. These monoclonal antibodies recognize surface antigens on haematopoietic precursor cells. Successful isolation of fetal cells was defined as detection of Y chromosomal sequences in maternal blood from women carrying male fetuses, with absence of Y sequences when female fetuses were carried. Thus, gender prediction accuracy was used as a measure of fetal cell separation. Using anti-CD 71 to isolate fetal cells, gender prediction was 57 per cent correct; with anti-CD 36, it was 88 per cent correct. Anti-GPA, an erythrocyte-specific antigen, used alone or in combination with anti-CD 71 or 36, improved gender prediction to 100 per cent. We conclude that antibody to GPA improves the retrieval of fetal NRBC from maternal blood, permitting genetic analysis by the polymerase chain reaction. 相似文献
253.
Lee P. Shulman MD Patricia L. Gordon Donald S. Emerson R. Sidney Wilroy Sherman Elias 《黑龙江环境通报》1993,13(5):403-409
We describe the prenatal diagnosis of isolated bilateral fetal microphthalmia in a woman at increased risk of having a fetus with microphthalmia. Ultrasound examinations at 161 and 19-5 weeks' gestation demonstrated bilateral fetal microphthalmia with no other associated structural defects. The patient elected to terminate her pregnancy at 19.5 weeks. Pathological evaluation of the products of conception obtained by dilation and evacuation confirmed the prenatal diagnosis of isolated bilateral fetal microphthalmia. 相似文献
254.
While true mosaicism occurs in only 0–25 per cent of genetic amniocenteses, nearly 2–5 per cent of amniotic fluid cell cultures contain a second cell line. In the common practice of prenatal diagnosis, an aberrant cell line confined to a single colony is usually disregarded. We present a case of mosaic trisomy 14 which was not detected on initial chromosome analysis. At birth, multiple malformations were apparent. Newborn cytogenetic studies revealed mosaicism [46,XX/46,XX,-14,+i(14q)] with an isochromosome 14 in 37 per cent of lymphocytes. Additional cells from the initial amniotic fluid culture were analysed post-delivery and the isochromosome 14 identified in only one of 12 total colonies. This case illustrates two important lessons in prenatal diagnosis. First, amniotic fluid cell cultures may not accurately reflect the relative distribution of the normal and abnormal cell lines within a mosaic fetus. Second, while it is generally reasonable to disregard mosaicism confined to a single colony, this policy will, on rare occasion, result in diagnostic error. This should be taken into consideration, particularly when dealing with autosomal trisomies potentially compatible with livebirth. 相似文献
255.
256.
257.
258.
Polyacetylene is presented as the prototype of a metallic conducting polymer. The concepts of one-dimensional metal physics are illustrated and special attention is drawn to a structural defect called “soliton”. Electrical, magnetic, and optical experiments connected with solitons are discussed. The authors' opinion is expressed that present experimental results do not yet furnish strong evidence for the existence of solitons in polyacetylene, but that the soliton idea is a fruitful stimulus for further investigations and novel applications. 相似文献
259.
260.