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751.
The effects of reduced oxygen concentrations in the gas phase over the culture medium on colony formation and cell proliferation were investigated in high and low cell density primary and secondary cultures of amniotic fluid cells. Using two standard culture methods (25 cm2 plastic flasks and Leighton type tubes) a significantly reduced culture time was observed at high cell density for mass cultures by incubation within a low oxygen tension gas phase (2.5 per cent to 7.5 per cent O2) instead of conventional air (18 per cent O2). At low cell density colony formation was significantly enhanced in cultures grown at reduced oxygen tension. Using gas permeable membranes as support, lowering the oxygen tension from 7.5 per cent to 2.5 per cent yielded an increase in plating efficiency of cells from approximately 5 per cent to 25 per cent, whereas plating efficiency was less than 2 per cent for cells grown at ambient 18 per cent O2. It is suggested that low oxygen tension in the gas phase is an effective means of enhancing clonal growth in amniotic fluid cell cultures, thereby reducing both culture time and risk of culture failure. 相似文献
752.
753.
Catherine Potvin Petra Tschakert Frédéric Lebel Kate Kirby Hector Barrios Judith Bocariza Jaime Caisamo Leonel Caisamo Charianito Cansari Juan Casamá Maribel Casamá Laura Chamorra Nesar Dumasa Shira Goldenberg Villalaz Guainora Patrick Hayes Tim Moore Johana Ruíz 《Mitigation and Adaptation Strategies for Global Change》2007,12(8):1341-1362
This paper is part of a two-year study to investigate the feasibility of initiating a Clean Development Mechanism (CDM) project
in an indigenous community of Eastern Panamá, Ipetí-Emberá. We use participatory mapping and matrices as well as household
surveys to develop a land-use/land-cover baseline scenario and examine the role of local participation in assessing land-use
change. In Ipetí, land-use change has not occurred in a linear way over the last decades, and our data unveils socio-economic
factors as potential key drivers of change. The concordance that we observed between geographic information and individual
and collective perceptions of land-use change substantiates the possibility of using local knowledge in the establishment
of baseline data for CDM projects. Our calculations suggest that the total carbon (C) stocks in the Tierra Colectiva (TC) of Ipetí-Emberá in 2004 represents a 47% reduction from the estimated C stock at the onset of settlement in the early
1970’s. Results from the participatory assessments predict that, in 2024 and in absence of a CDM project, the C stocks will
decline from 301,859 t C in 2004 to 155,730 t C, which constitutes a reduction of 52%. The scenario with CDM estimates C stocks
of 305,853 t C for 2024, a value slightly superior to the 2004 value. In the TC there is ground to believe that cattle ranching
is likely to become an ever more important activity as the population is young and growing and cannot easily move elsewhere.
Forests tend to be cleared for cultivation while pastures are established on short fallows. Our baseline scenario underlines
the potential for a CDM project to make a significant difference in the future C stocks of this landscape. 相似文献
754.
M. Schwartz M. Super J. Schmidtke C. Buys M. Farrall D. Halley M. Krawczak J. E. Poncin D. Loukopoulos M. Devoto 《黑龙江环境通报》1988,8(8):619-624
This paper presents data collected in Europe on 107 prenatal diagnoses of cystic fibrosis (CF) using linked DNA markers. To date, 38 children have been born without CF, as predicted, demonstrating the present rapid move from research to clinical genetic service. 相似文献
755.
High-performance permanent magnets 总被引:2,自引:0,他引:2
High-performance permanent magnets (pms) are based on compounds with outstanding intrinsic magnetic properties as well as
on optimized microstructures and alloy compositions. The most powerful pm materials at present are RE–TM intermetallic alloys
which derive their exceptional magnetic properties from the favourable combination of rare earth metals (RE=Nd, Pr, Sm) with
transition metals (TM=Fe, Co), in particular magnets based on (Nd,Pr)2Fe14B and Sm2(Co,Cu,Fe,Zr)17. Their development during the last 20 years has involved a dramatic improvement in their performance by a factor of >15 compared
with conventional ferrite pms therefore contributing positively to the ever-increasing demand for pms in many (including new)
application fields, to the extent that RE–TM pms now account for nearly half of the worldwide market. This review article
first gives a brief introduction to the basics of ferromagnetism to confer an insight into the variety of (permanent) magnets,
their manufacture and application fields. We then examine the rather complex relationship between the microstructure and the
magnetic properties for the two highest-performance and most promising pm materials mentioned. By using numerical micromagnetic
simulations on the basis of the Finite Element technique the correlation can be quantitatively predicted, thus providing a
powerful tool for the further development of optimized high-performance pms. 相似文献
756.
The application of ultrasonic-electrolysis process for the removal of copper is studied.In the ultrasonic field cavitation acts as jets and agitates the solution and breaks the barrier layer between the cathode surface and the bulk of the solution.Thus increases metal deposition on the cathode surface.The results show that an ultrasonic field is successful for the removal of low copper concentrations in solution. 相似文献
757.
CLIMATE CHANGE AND NATURAL HAZARDS IN NORTHERN CANADA: INTEGRATING INDIGENOUS PERSPECTIVES WITH GOVERNMENT POLICY 总被引:1,自引:0,他引:1
John?NewtonEmail author C.?D.?James?Paci Aynslie?Ogden 《Mitigation and Adaptation Strategies for Global Change》2005,10(3):541-571
A study of the relationship between natural hazards and climate change in the international context provides the background
for a discussion of the expected changes. In the context of this global discussion, this paper reviews the current perspectives
of those natural hazards that are likely to be influenced by climate change, using northern Canada as a regional case study.
The northern implications of the United Nations Framework Convention on Climate Change are examined, including the status
of climate change action by the northern territorial governments, the evolving role of indigenous people, and the responsibility
for climate change impacts. The difficulties surrounding natural hazards research in remote locations, and the approaches
of indigenous people to natural hazards are then presented. The paper concludes with a suggested policy approach for climate
change and natural hazards in northern Canada, underscoring the need for more comprehensive adaptive strategies to complement
the current tendency to focus on the mitigation of greenhouse gases produced in this region. 相似文献
758.
759.
To gain an understanding of the characteristics of the needle surfaces of naturally regenerated Scots pine (Pinus sylvestris L.) and their geographical distribution, eight physicochemical variables were investigated within the north boreal forest area. The visibly undamaged needles were collected in autumn 1990 from 114 plots (3-5 pines per plot) along radial transects from the Monchegorsk and Nikel smelters, emitting SO2 and heavy metals, on the Kola Peninsula, Russia, to Finnish Lapland. The needles were examined by scanning electron microscopy (SEM) and measured for surface wettability using the droplet contact angle (DCA) method. Significant geographical south-north and west-east patterns could be observed in needle surface characteristics, correlating with emissions from the smelters and to climate. Despite the slightly higher initial DCAs (61.4-87.6 degrees) towards the north, the pine needle surfaces of the northernmost transects exhibited higher annual needle wettability change (ANWC) and wax erosion rate (AWER), expressed as the reduction in DCAs and epistomatal wax tube distribution (WTD) during one year, respectively, but a lower occurrence of particles and fungal hyphae than those of more southern transects. The higher ANWC was related to higher atmospheric SO2 concentration, and to the lower long-term temperature sum, but not clearly to annual precipitation. In the Monchegorsk smelter area, the current needles exhibited, on average, a 15% higher WTD and seven degree larger DCA, resulting in more hydrophobic needle surfaces than in Finnish Lapland, but during their first year, both the AWER and ANWC, were greatly increased. In Finnish Lapland, 30%, of all the epistomatal wax tubes disappeared from the needle surface during the first year, the value being 70% for the pines located 8 km from the smelter. The mineral composition of the particles deposited on the needle surfaces mirrored that of the minerals being produced by the smelters (e.g. FexSx, CuFeS2, NixSx, FexOx). Stomatal densities were in the range of 71.1-141.7 stomata mm(-2). The lower densities of stomata on needles close to the smelters correlated with reduced number of needle age classes of the pines, higher dry weights, higher pollutant accumulation and lower Mn and Zn concentrations in the needles. The AWER and ANWC were able to indicate the most seriously deteriorated needle surfaces on a regional scale, e.g. including the surroundings of both smelters, although the plot-specific relationships between 'needle surface variables' and 'pollution variables' were generally weak. The present study showed that the exposure of pine needles to the combined effects of ambient pollutants and harsh climate led to a deterioration in the physicochemical characteristics of the epicuticular wax, which may have serious ecophysiological consequences in the long term. 相似文献
760.
Skelly JM Fredericksen TS Savage JE Snyder KR 《Environmental pollution (Barking, Essex : 1987)》1996,94(2):235-240
Ambient concentrations of ozone (O(3)) and carbon dioxide (CO(2)) were measured at locations from the forest floor to the top of the canopy in a deciduous forest at the Moshannon State Forest in northcentral Pennsylvania. O(3) concentrations were measured from May-September for three years (1993-1995) while CO(2) concentrations were measured only during July and August of 1994. O(3) concentrations increased steadily during the day at all locations, peaking during the middle to late afternoon hours. O(3) concentrations then steadily declined to their lowest point, just before dawn. Vertical O(3) concentration gradients varied seasonally and among years. However, O(3) concentrations were highest within the forest canopy and lowest at the forest floor, with an average difference of approximately 13%. Differences in O(3) concentrations between the canopy and forest floor were greatest at night. O(3) concentrations were slightly higher at locations within the canopy than above the canopy. CO(2) concentrations were consistenly higher near the forest floor and were higher above the canopy than within the canopy. CO(2) concentrations were higher at night than during the day at all locations, especially near the forest floor. 相似文献