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101.
102.
A method was developed for measurement of active oxygen production in embryonic stages of the brown seaweed Fucus spiralis, using the label CM-DCFH-DA. Active oxygen species convert the label into the green fluorescent CM-DCF (exc/em 488/530 nm)
that is detected via confocal laserscan microscopy and quantitative image analysis. Loading of the label did not harm the
embryos; loading efficiency was age-independent, and the esterases needed for conversion to CM-DCFH were not inhibited by
the effective UV dose (2 W m−2) applied in the experiments. After correction for daily variation of the laser power, and calibration with DCF standard solutions,
this automated analysis of confocal images rendered active oxygen concentrations in fucoid embryos (μM DCF). An experiment
was designed for the assessment of active oxygen production following irradiance stress in the light-sensitive embryos. Dim-light-acclimated,
1-, 2- and 4-day-old embryos were transferred for 60 min to defined high-light conditions (4π-irradiance 300 μmol photons
m−2 s−1), optionally without UV radiation, including UVA, or including UVA plus UVB. PSII yield measurements (PAM fluorometer) were
carried out in order to assess the degree of photoinhibition under these light conditions. The imposed light stress initially
caused a rapid decrease of the PSII yields (ΦP). With increasing embryo age, minimum ΦP values attained under light stress remained higher. Consequently, electron transport rates (ETR) would increase with embryo
age, i.e., with the development of their photosynthetic apparatus. Active oxygen production increased with ETR, and when UVB
was included, relatively greater amounts of active oxygen were produced. A slow, second-phase decrease of ΦP under light stress that was proportional to active oxygen production indicated that some photooxidative damage was caused,
in particular during UVB exposure. Recovery from light stress was a rapid process in the absence of UVB; in such cases ΦP was almost restored to the initial values within 60 min. The relative state of recovery of ΦP was correlated with both the effective UV dose and active oxygen production rate (DCF). Recovery was slowest in embryos exposed
for 60 min to an experimental UVB dose, which was representative of a situation at low tide, on a sunny day. The results suggest
that active oxygen may cause an in situ inhibition of growth of the earliest life stages of F. spiralis.
Received: 26 January 2000 / Accepted: 4 September 2000 相似文献
103.
Y. Nassiri P. S. Rainbow C. Amiard-Triquet F. Rainglet B. D. Smith 《Marine Biology》2000,136(3):477-484
The talitrid amphipod crustacean Orchestia gammarellus (Pallas) was collected from metal-contaminated (Dulas Bay, Gironde) and control (Millport) sites in the UK and France. Irrespective
of site of origin, the amphipods showed the same physiological mechanism of trace-metal detoxification, involving the ventral
caeca. Copper was always present in lysosomal residual bodies in the ventral caeca. Following laboratory exposure to zinc
and cadmium, the lysosomes usually contained both copper and zinc but cadmium was not detectable. The lysosomal copper concentration
is positively correlated to that of sulphur, while the concentration of lysosomal zinc is related to that of phosphorus. Results
are interpreted in terms of the differential rates of turnover of metallothioneins chelating copper, zinc or cadmium.
Received: 9 February 1999 / Accepted: 2 December 1999 相似文献
104.
Biodiversity and Disease Risk: the Case of Lyme Disease 总被引:14,自引:0,他引:14
105.
Genetic structure of fissiparous populations of Holothuria (Halodeima) atra on the Great Barrier Reef 总被引:1,自引:0,他引:1
Allozyme variation at five polymorphic loci was surveyed in a total of 311 individuals of the sea cucumber Holothuria (Halodeima) atra (Jäger, 1833) collected from two nearshore and two midshelf populations in the Great Barrier Reef in November 1996. Strong deviations in genotype frequencies from those expected under Hardy–Weinberg equilibrium, particularly a large number of heterozygote excesses, confirmed the occurrence of asexual reproduction. Females and males differed significantly in genotype frequencies as a result of differences in either the amount of fission in each sex or in the mortality of asexually produced recruits. The estimated maximum sexual input (number of sexually produced individuals: sample size = N*:Ni) to the two nearshore reefs (38 to 67%) was low relative to that to the two midshelf reefs (74 to 87%). The three ratios and G o :G e , N go :N i , N*:N i , (where G o = observed genotypic diversity, G e = expected genotypic diversity, N go = number of genotypes) considered to be indicators of the extent of asexual reproduction, showed a consistent trend in the degree of asexual reproduction similar to that derived from the number of regenerating individuals observed in the populations for which data were available. F-statistic analyses of clonal gene frequencies demonstrated that all populations received sexual recruits from the same gene pool. There was evidence of restricted sexual recruitment to the Fantome population, suggesting that asexual reproduction was dominant only in areas where sexual recruitment was limited by other factors. 相似文献
106.
Use of otoliths and eye lenses for measuring trace-metal incorporation in fishes: a biogeographic study 总被引:1,自引:0,他引:1
The otoliths and lenses of the temperate damselfish Parma microlepis (Günther) (Pomacentridae) showed similar differences in trace-metal profile for selected locations along the coast of New
South Wales, Australia. Otoliths and lenses displayed a differential ability to accumulate metals. Metal concentrations were
ranked differently in the two structures (e.g. Sr > Ba > Pb > Rb > Hg in otoliths, and Hg > Sr ≃ Rb > Pb > Ba in lenses),
and where similar metals were accumulated, they were accumulated at vastly different concentrations (e.g. Ba concentrations
in otoliths are a thousand-fold greater than in lenses). Analyses of the otoliths and lenses of P. microlepis from locations close to Sydney and up to 100 km from the city were able to distinguish amongst these locations with respect
to a number of metals, namely Ba, Mn and Hg. Multivariate analyses of otolith and lens data gave similar results among locations
(agreement was obtained for 11 out of 15 pair-wise comparisons), and differences were attributable to the differential ability
of the two structures to accumulate metals such as Mn and Hg. Trace-metal differences between locations were found to coincide
with the proximity of sewage (including industrial waste) and petroleum storage facilities to the different locations.
Received: 23 June 1997 / Accepted: 4 August 1997 相似文献
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