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941.
Hepatitis A virus (HAV) is currently recognized as an important human food borne pathogen, and it is one of the most resistant
enteric RNA viruses, is highly infectious, and may lead to widespread outbreaks. The aim of this study was to optimize the
methods to detect HAV from artificially contaminated food. To this end, strawberry and lettuce were experimentally contaminated
with HAV suspension containing 6 × 106 copies/ml. After contamination, HAV persistence and washing procedure were evaluated at 0, 1, 3, 7, and 9 days of storage.
Five elution buffers (PBS (pH 7.4)/0.1% Tween80; 50 mM glycine/3% (wt/vol) beef extract (pH 9.5); PBS (pH 7, 4); 25 mM glycine/0.1
Tween80; and 1 M sodium bicarbonate) were used to elute the virus, and qualitative and quantitative PCR were used for HAV
detection. HAV was detected by qualitative and quantitative PCR using any of the five elution buffers, but PBS was the most
effective. Even after washing, HAV was detected up to 9 days after contamination by quantitative PCR. Quantitative PCR was
more sensitive than qualitative PCR since samples containing viral load lower than 1.4 × 103 copies/ml could not be detected by qualitative PCR. Quantitative PCR can be used for rapid detection of food borne viruses
and will help in the monitoring and control of food borne disease. 相似文献
942.
Kattarkandi Byjesh Soora Naresh Kumar Pramod Kumar Aggarwal 《Mitigation and Adaptation Strategies for Global Change》2010,15(5):413-431
Climate change associated global warming, rise in carbon dioxide concentration and uncertainties in precipitation has profound
implications on Indian agriculture. Maize (Zea mays L.), the third most important cereal crop in India, has a major role to play in country’s food security. Thus, it is important
to analyze the consequence of climate change on maize productivity in major maize producing regions in India and elucidate
potential adaptive strategy to minimize the adverse effects. Calibrated and validated InfoCrop-MAIZE model was used for analyzing
the impacts of increase in temperature, carbon dioxide (CO2) and change in rainfall apart from HadCM3 A2a scenario for 2020, 2050 and 2080. The main insights from the analysis are threefold.
First, maize yields in monsoon are projected to be adversely affected due to rise in atmospheric temperature; but increased
rainfall can partly offset those loses. During winter, maize grain yield is projected to reduced with increase in temperature
in two of the regions (Mid Indo-Gangetic Plains or MIGP, and Southern Plateau or SP), but in the Upper Indo-Gangetic Plain
(UIGP), where relatively low temperatures prevail during winter, yield increased up to a 2.7°C rise in temperature. Variation
in rainfall may not have a major impact on winter yields, as the crop is already well irrigated. Secondly, the spatio-temporal
variations in projected changes in temperature and rainfall are likely to lead to differential impacts in the different regions.
In particular, monsoon yield is reduced most in SP (up to 35%), winter yield is reduced most in MIGP (up to 55%), while UIGP
yields are relatively unaffected. Third, developing new cultivars with growth pattern in changed climate scenarios similar
to that of current varieties in present conditions could be an advantageous adaptation strategy for minimizing the vulnerability
of maize production in India. 相似文献
943.
Enrico Cappellini M. Thomas P. Gilbert Filippo Geuna Girolamo Fiorentino Allan Hall Jane Thomas-Oates Peter D. Ashton David A. Ashford Paul Arthur Paula F. Campos Johan Kool Eske Willerslev Matthew J. Collins 《Die Naturwissenschaften》2010,97(2):205-217
We report here the first integrated investigation of both ancient DNA and proteins in archaeobotanical samples: medieval grape (Vitis vinifera L.) seeds, preserved by anoxic waterlogging, from an early medieval (seventh–eighth century A.D.) Byzantine rural settlement in the Salento area (Lecce, Italy) and a late (fourteenth–fifteenth century A.D.) medieval site in York (England). Pyrolysis gas chromatography mass spectrometry documented good carbohydrate preservation, whilst amino acid analysis revealed approximately 90% loss of the original protein content. In the York sample, mass spectrometry-based sequencing identified several degraded ancient peptides. Nuclear microsatellite locus (VVS2, VVMD5, VVMD7, ZAG62 and ZAG79) analysis permitted a tentative comparison of the genetic profiles of both the ancient samples with the modern varieties. The ability to recover microsatellite DNA has potential to improve biomolecular analysis on ancient grape seeds from archaeological contexts. Although the investigation of five microsatellite loci cannot assign the ancient samples to any geographic region or modern cultivar, the results allow speculation that the material from York was not grown locally, whilst the remains from Supersano could represent a trace of contacts with the eastern Mediterranean. 相似文献
944.
Silvia H. Bonilla Rodrigo L. Guarnetti Cecilia M.V.B. Almeida Biagio F. Giannetti 《Journal of Cleaner Production》2010,18(1):83-91
Bamboo presents physical and mechanical characteristics, which turn it an alternative option for product development, replacing native or reforested wood. The sustainability assessment of a Dendrocalamus Giganteus species plantation in Brazil through the emergy methodology evidences a great weight of renewable (30% sej/sej) and human labour contributions (33% sej/sej). These contributions account for the great interface with environment and to the intensive work, respectively. The transformity value of bamboo production is 2.42E + 04 sej/J. The influence human labour has on the total emergy flow and on indicators is evaluated by taking into account different country locations (Brazil, Australia and China). Thus, a different transformity value for labour is assumed for each country. A ranking based on emergy sustainability index (ESI) values shows that bamboo production in China was the first placed, followed by Brazil and Australia (values of 1.18, 0.50 and 0.09, respectively). The insertion of indirect renewability embedded in labour results in the ranking modification, leading to plantation in Brazil in the first place, followed by the Australian and Chinese ones. The relative position of the bamboo systems is visualized in the ternary diagram expressed in terms of emergy. In an attempt to explore the relationship between sustainability and time, a graphic of ESI vs. global productivity is discussed in terms of a prospective evaluation. Indirect support areas of the bamboo production are calculated as a way to evaluate the sustainability-space relationship. 相似文献
945.
946.
947.
Luis Lassaletta Héctor García-Gómez Benjamín S. Gimeno José V. Rovira 《Environmental Science & Policy》2010,13(5):423-433
The European Union Water Framework Directive (WFD) aims to achieve the “good status” of waters by 2015, through monitoring and control of human impacts on “bodies of surface water” (BSWs), discrete elements for quality diagnosis and management. Headwater streams, however, are frequently neglected as they are not usually recognised as BSW. This poses limitations for the management of river catchments, because anthropogenic impacts on headwaters can constrain the quality of downstream rivers. To illustrate this problem, we compared nitrate levels and land use pressures in a small agricultural catchment with those recorded in the catchment in which it is embedded (Ega), and in the Ebro River Basin (NE Spain) comprising both. Agriculture greatly influenced water nitrate concentration, regardless of the size of the catchments: R2 = 0.91 for headwater catchments (0.1–7.3 km2), and R2 = 0.82 for Ebro tributary catchments (223–3113 km2). Moreover, nitrate concentration in the outlet of a non-BSW small river catchment was similar to that of the greater downstream BSW rivers. These results are of interest since, despite representing 76% of the length of the Ega catchment hydrographical network, only 3.1% of the length of the headwater streams has been identified as BSWs. Human activities affecting headwater streams should therefore be considered if the 2015 objective of the WFD is to be achieved. 相似文献
948.
Cribb BW Stewart A Huang H Truss R Noller B Rasch R Zalucki MP 《Die Naturwissenschaften》2008,95(5):433-441
Previously, the presence of metals in arthropod mandibles has been linked with harder cuticle, and in termites, a 20% increase in hardness has been found for mandibles containing major quantities of zinc. The current study utilises electron microscopy and energy-dispersive X-ray microanalysis to assess incidence and abundance of metals in all extant subfamilies of the Isoptera. The basal clades contain no zinc and little to no manganese in the cutting edge of the mandible cuticle, suggesting that these states are ancestral for termites. However, experimentation with mandibles in vitro indicates the presence of some elements of the cuticular biochemistry necessary to enable uptake of zinc. The Termopsidae, Serritermitidae, Rhinotermitidae and Termitidae all contain minor quantities of manganese, while trace to minor quantities of zinc occur in all except the Serritermitidae. In contrast, all Kalotermitidae or drywood termites contain major levels of zinc in the mandible edge. Diet and life type are explored as links to metal profiles across the termites. The presence of harder mandibles in the drywood termites may be related to lack of access to free water with which to moisten wood. Scratch tests were applied to a set of mandibles. The coefficient of friction for Cryptotermes primus (Kalotermitidae) mandibles, when compared with species from other subfamilies, indicates that zinc-containing mandibles are likely to be more scratch resistant. 相似文献
949.
Ants of the genus Camponotus are able to discriminate recognition cues of colony members (nestmates) from recognition cues of workers of a different colony (non-nestmates) from a distance of 1 cm. Free moving, individual Camponotus floridanus workers encountered differently treated dummies on a T-bar and their behavior was recorded. Aggressive behavior was scored as mandibular threat towards dummies. Dummies were treated with hexane extracts of postpharyngeal glands (PPGs) from nestmates or non-nestmates which contain long-chain hydrocarbons in ratios comparable to what is found on the cuticle. The cuticular hydrocarbon profile bears cues which are essential for nestmate recognition. Although workers were prevented from antennating the dummies, they showed significantly less aggressive behavior towards dummies treated with nestmate PPG extracts than towards dummies treated with non-nestmate PPG extracts. In an additional experiment, we show that cis-9-tricosene, an alkene naturally not found in C. floridanus' cuticular profile, is behaviorally active and can interfere with nestmate recognition when presented together with a nestmate PPG extract. Our study demonstrates for the first time that the complex multi-component recognition cues can be perceived and discriminated by ants at close range. We conclude that contact chemosensilla are not crucial for nestmate recognition since tactile interaction is not necessary. 相似文献
950.