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排序方式: 共有182条查询结果,搜索用时 203 毫秒
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The Science of Nature - 相似文献
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Partitioning radiolabeled thymidine uptake by bacteria and meiofauna using metabolic blocks and poisons in benthic feeding studies 总被引:2,自引:0,他引:2
Techniques exist which allow for the measurement of in situ grazing rates of meiobenthos on sedimentary bacteria and microalgae. Radiolabeled substrates are incorporated into microbes which serve as food for meiofauna and which themselves also become labeled during feeding. However, during in situ grazing experiments, meiofauna may become radiolabeled by a variety of non-feeding processes. Proper controls to correct for these extraneous routes of labeling have been developed in the present study. The use of [methyl-3H] thymidine (3HTdR) in studies of meiofaunal grazing on bacteria has two unique advantages: (1) it is incorporated only into prokaryotic macromolecules, and (2) bacterial incorporation of 3HTdR may be selectively blocked by several inhibitors which are non-toxic to meiofaunal grazers. Coupled with formalin-killed control treatments, the use of these inhibitors makes it possible to accurately determine the partitioning of radiolabel into meiofauna during grazing into adsorptive, absorptive and grazing components. A saturated solution of nalidixic acid and 5-deoxythymidine was found to be most effective in inhibiting water-column bacterial uptake and incorporation of 3HTdR, and had no toxic effects on meiofauna. The inhibitor was found to immediately block bacterial incorporation of 3HTdR and was as effective at 20% saturation as at 100%. The presence of sediment reduced the effectiveness of this inhibitor by 50%. Solutions of the inhibitor with excess undissolved material, however, completely blocked sediment bacterial uptake of 3HTdR. Employing these techniques during in situ grazing experiments showed that up to 83% of total meiofaunal uptake of 3H-label may be attributable to non-grazing processes. Experiments conducted in slurried sediments yielded grazing rates which were the same as those from intact cores. Furthermore, meiofaunal grazing rates on multiple food sources (e.g. bacteria and diatoms) may be determined synoptically by adding isotopically-distinct substrates (e.g. 3HTdR and H14CO3) to the same experimental incubation.University of Texas Marine Science Contribution No. 698. 相似文献
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The effect of wastewater treatment on antibiotic resistance in Escherichia coli and Enterococcus sp.
Sarah Garcia Bradley Wade Carrie Bauer Carri Craig Karen Nakaoka William Lorowitz 《Water environment research》2007,79(12):2387-2395
The effects of wastewater treatment on the proportion of Escherichia coli and Enterococcus sp. resistant to specific antibiotics were investigated at two facilities in Davis County, Utah, one of which received hospital waste. Samples were taken from the influent, effluent before disinfection, and secondary anaerobic sludge digester effluent. There was very little difference in antibiotic resistance among E. coli in the inflow waters of the plants but the plant receiving hospital waste had a significantly higher proportion of antibiotic resistant Enterococcus. The effect of wastewater treatment on antibiotic resistance was more pronounced on enterococci than E. coli. Although some increases in antibiotic resistance were observed, the general trend seemed to be a decrease in resistance, especially in the proportion of multidrug resistant Enterococcus sp. 相似文献
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Heterogeneous photocatalytic oxidation of organics for air purification by near UV irradiated titanium dioxide 总被引:4,自引:0,他引:4
The photocatalytic degradation of high concentrations of various organic pollutants (acetone, 2-propanol and toluene) in dry and humid air streams was carried out using a specially designed photoreactor based on the UV-TiO2 principle. The influence of several parameters which control the destruction efficiency (flow rate, initial contaminant and water vapour concentration, temperature and light intensity) has been studied. The conversion was maximal at room temperature, low flow rates and low initial contaminant concentrations. The presence of water in the inlet stream strongly affected the performance of the catalyst. The primary oxidation product of 2-propanol was acetone. 相似文献
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