Monitoring of hexabromocyclododecane diastereomers in fish from European freshwaters and estuaries

Background and aims  

Hexabromocyclododecane (HBCD) is a brominated flame retardant used mainly in polystyrene foam as well as in textile applications. In recent years, measures were taken to reduce HBCD emissions during its production and use. To evaluate the efficacy of these measures, a monitoring project was initiated with fish as bioaccumulation indicators.     

Assessment of exposure to DDT and metabolites after indoor residual spraying through the analysis of thatch material from rural African dwellings

Introduction  

We report on the analysis of 4,4′-dichlorodiphenyltrichloroethane (4,4′-DDT) and its metabolites in thatch and branch samples constituting the wall materials of dwellings from South African subtropical areas. This approach was used to assess the exposure to DDT in the residents of the dwellings after indoor residual spraying (IRS) following recommended sanitation practices against malaria vectors.     

An observational study on changes in biometry and generation time of <Emphasis Type="Italic">Odontophora villoti</Emphasis> (Nematoda,Axonolaimidae) related to petroleum pollution in Bizerte bay,Tunisia

Introduction  

We conducted a yearly polluted-reference sampling to assess the effects of petroleum pollution on life cycle characteristics of the meiobenthic nematode Odontophora villoti. Samples were taken every 15 days between 26 November 2004 and 25 November 2005 from two beaches of Bizerte bay (Tunisia), Rimel and Tunisian Refining Industries Company (TRIC). The latter site is located in front of the “Tunisian Refining Industries Company” runoff.     

Pesticide removal from waste spray-tank water by organoclay adsorption after field application: an approach for a formulation of cyprodinil containing antifoaming/defoaming agents

Purpose  

Many reports on purification of water containing pesticides are based on studies using unformulated active ingredients. However, most commercial formulations contain additives/adjuvants or are manufactured using microencapsulation which may influence the purification process. Therefore, the main objective of this work was to develop and test a pilot scheme for decontaminating water containing pesticides formulated with antifoaming/defoaming agents.     

Apple snails and their endosymbionts bioconcentrate heavy metals and uranium from contaminated drinking water

Purpose

The differential ability of apple snail tissues, endosymbionts, and eggs to bioaccumulate several metals (Sb, As, Ba, Br, Zn, Cr, Fe, Hg, Se, and U) was investigated.

Methods

Metal concentrations were determined by neutron activation analysis in several tissues, endosymbionts, and eggs from mature apple snails cultured in either drinking water or reconstituted water (prepared with American Society for Testing and Materials type I water).

Results

The highest bioconcentration factors (BCFs) in the midgut gland were found for Ba, Zn, Se, As, U, Br, and Hg (in decreasing order), while the highest in the kidney were for Ba, Br, and Hg. The foot showed the highest BCFs for Ba, Hg, Br, and Se (in decreasing order). Calcified tissues (uterus, shell) and eggs showed low BCFs, except for Ba. Both C corpuscles and gland tissue showed statistically higher BCFs than K corpuscles for Ba, Fe, U, Br, and Sb. The concentration of most of the studied elements was significantly lower in tissues and endosymbionts obtained from snails cultured in reconstituted water instead of drinking water. Snails cultured in reconstituted water and then exposed or not to Hg, As, and U (at the maximum contaminant level allowed by the US Environmental Protection Agency) also resulted in high levels accumulated in midgut gland, endosymbionts and kidney.

Conclusions

Our findings suggest that the midgut gland (and the symbionts contained therein), the kidney, and the foot of Pomacea canaliculata may be useful bioindicators of Hg, As and U pollution in freshwater bodies and that the unrestricted use of ampullariid snails as human and animal food must be considered with caution..     

Iophenoxic acid derivatives as markers of oral baits to wildlife

The bait-marker iophenoxic acid (IPA) and its derivatives are increasingly used for evaluating and optimizing the cost-effectiveness of baiting campaigns on wildlife, particularly on game species such as the wild boar. We aimed to determine whether concentrations of the three main IPA derivatives ethyl, methyl and propyl-IPA measured on thoracic liquid extracts (TLE) of hunted wild boars may be representative of two exposure doses, 40 and 200 mg, from 20 to 217 days after ingestion. Then we developed a method of detection of the three IPA derivatives by LC/ESI-MS-MS in muscle and liver to evaluate the suitability of these two other tissues for monitoring the marked bait consumption and for measuring available residues in the meat of marked animals. Three semi-captive wild boars received 40 mg of each IPA derivative, three received 200 mg, and three, as controls, did not receive IPA. Blood serum was sampled 20, 197 or 217 days after IPA exposure according to animals and to the derivative. Wild boars were shot by gun after the different times of serum sampling times, and TLE, muscle and liver were sampled. Our results suggest that TLE is not a relevant tissue for quantitatively expressing IPA exposure. Due to interference, no analytical method was validated on TLE containing digestive material. On the other hand, quantifications in the muscle and particularly in the liver could discriminate wild boars that had ingested the two IPA doses from 20 days until 7 months after exposure, especially for the two long term markers ethyl and propyl-IPA. So IPA quantifications in the liver sampled on hunted animals appear to be a reliable tool for monitoring bait consumption in the field at a large scale. Nevertheless, whatever the ingested dose, ethyl- and propyl-IPA concentrations measured in the muscle and the liver of tested animals until 217 days after exposure, remained higher than 0.01 mg/kg, the Maximal Residue Limit (MRL) is recommended for molecules for which no toxicological data are available. Based on the range of IPA residues available in these two tissues, implications for humans consuming marked animals are discussed.     

Capacity of biochar application to maintain energy crop productivity: soil chemistry, sorghum growth, and runoff water quality effects

Pyrolysis of crop biomass generates a by-product, biochar, which can be recycled to sustain nutrient and organic C concentrations in biomass production fields. We evaluated effects of biochar rate and application method on soil properties, nutrient balance, biomass production, and water quality. Three replications of eight sorghum [ (L.) Moench] treatments were installed in box lysimeters under greenhouse conditions. Treatments comprised increasing rates (0, 1.5, and 3.0 Mg ha) of topdressed or incorporated biochar supplemented with N fertilizer or N, P, and K fertilizer. Simulated rain was applied at 21 and 34 d after planting, and mass runoff loss of N, P, and K was measured. A mass balance of total N, P, and K was performed after 45 d. Returning 3.0 Mg ha of biochar did not affect sorghum biomass, soil total, or Mehlich-3-extractable nutrients compared to control soil. Yet, biochar contributed to increased concentration of dissolved reactive phosphorus (DRP) and mass loss of total phosphorus (TP) in simulated runoff, especially if topdressed. It was estimated that up to 20% of TP in topdressed biochar was lost in surface runoff after two rain events. Poor recovery of nutrients during pyrolysis and excessive runoff loss of nutrients for topdressed biochar, especially K, resulted in negative nutrient balances. Efforts to conserve nutrients during pyrolysis and incorporation of biochar at rates derived from annual biomass yields will be necessary for biochar use in sustainable energy crop production.     

Invasion of Pinus halepensis Mill. following a wildfire in an Argentine grassland nature reserve

The Ernesto Tornquist nature reserve is a relict of native Pampas vegetation in Argentina. Alien trees were introduced to the reserve in the 1950s, mainly to "improve" the natural landscape, resulting in the arrival of a totally new life form. In 1987, a fire affected an area planted with Pinus halepensis resulting in its massive expansion. In 1999, we removed trees from 17 circular plots of 10 m diameter placed systematically within the area that was colonized after the fire. Trunks were cut 20 cm from the ground and growth rings were counted. We studied the age structure of the population in order to reconstruct the colonizing events after the fire. We found that recruitment occurred throughout this period, except in the three years after the disturbance. We suggest that this delay in recruitment might be caused by low seedling survival under water stress conditions due to low rainfall, combined with scarce vegetation cover after fire. This could have been associated with an initial reduction in propagule pressure due to the scarcity of surviving trees in the vicinity and with the fact that fire occurred after the peak of seed release, during an extremely dry summer, probably killing a great number of seeds that were already in the soil. In the following years, recruitment was probably aided by pioneer trees and later by seeds shed from established pines. Alien trees had been allowed to reach maturity due to wildfire prevention and control in the years preceding the fire and the accumulated dry matter resulted in increased fire intensity that reduced the ability of grasses to re-sprout. As a consequence, the invasion window that allowed the expansion of pines remained open for at least 12 years.     

Evolution of a Mediterranean Coastal Zone: Human Impacts on the Marine Environment of Cape Creus

This study presents an integrated analysis of the evolution of the marine environment and the human uses in Cape Creus, a Mediterranean coastal area where intense commercial fisheries and recreational uses have coexisted over the last fifty years. The investigation synthesizes the documented impacts of human activities on the marine environment of Cap de Creus and integrates them with new data. In particular, the evolution of vulnerable, exploited species is used to evaluate the fishing impacts. The effects of area protection through the establishment of a marine reserve in the late 1990s and the potential climate change impacts are also considered. The evolution of the human uses is marked by the increasing socioeconomic importance of recreational activities (which affect species and habitats) in detriment to artisanal and red coral fisheries (which principally affect at a species level). Overall, populations of sedentary, vulnerable exploited species, hard sessile benthic invertebrates, and ecologically fragile habitats, such as seagrass meadows, the coralligenous and infralittoral algal assemblages have been the most negatively impacted by anthropogenic activities. Albeit human uses currently constitute the largest negative impact on the marine environment of Cap de Creus, climate change is emerging as a key factor that could have considerable implications for the marine environment and tourism activities. The establishment of the marine reserve appears to have had little socioeconomic impact, but there is some evidence that it had some positive biological effects on sedentary, littoral fishes. Results demonstrate that the declaration of a marine reserve alone does not guarantee the sustainability of marine resources and habitats but should be accompanied with an integrated coastal management plan.     

Making Capacity Building Meaningful: A Framework for Strategic Action

This paper aims to give practical meaning to ‘capacity building’ through (a) identifying a suite of practical measures, such as mentoring or best practice guidelines, that have been shown to or are considered to build human, social, institutional, and economic capital; (b) placing these measures within a broader systems framework; and (c) exploring stakeholder feedback on specific measures to inform framework implementation. The 29 measures described provide actors, whether government or nongovernment, with a suite of practical investment choices for building capacity. These measures are then clustered into eight groups according to their primary purpose and placed within a systems framework. The framework provides a tool for actors with responsibilities for or an interest in capacity building to inform more holistic and strategic targeting of effort and investment. Stakeholder feedback gathered through surveys and workshops is subsequently reported to further inform implementation of specific measures within the framework’s eight groupings. The framework presented may be built upon through the identification and inclusion of further capacity building measures. The research is conducted within the context of decentralized governance arrangements for natural resource management (NRM), with specific focus on Australia’s recently formalized 56 NRM regions and their community-based governing boards as an informative arena of learning. Application of the framework is explored in the Australian setting through the identification and comparison of measures supported and most preferred by four major stakeholder groups, namely board members, regional NRM organization staff, policy/research interests, and Indigenous interests. The research also examines stakeholder perceptions of capacity issues, and whether these issues are likely to be addressed through implementing their preferred measures.