Satellite-based decadal change assessments of pan-Arctic environments

Remote sensing can advance the work of the Circumpolar Biodiversity Monitoring Program through monitoring of satellite-derived terrestrial and marine physical and ecological variables. Standardized data facilitate an unbiased comparison across variables and environments. Using MODIS standard products of land surface temperature, percent snow covered area, NDVI, EVI, phenology, burned area, marine chlorophyll, CDOM, sea surface temperature, and marine primary productivity, significant trends were observed in almost all variables between 2000 and 2017. Analysis of seasonal data revealed significant breakpoints in temporal trends. Within the terrestrial environment, data showed significant increasing trends in land surface temperature and NDVI. In the marine environment, significant increasing trends were detected in primary productivity. Significantly earlier onset of green up date was observed in bioclimate subzones C&E and longer end of growing season in B&E. Terrestrial and marine parameters showed similar rates of change with unidirectional change in terrestrial and significant directional and magnitude shifts in marine.     

Effects of preference heterogeneity among landowners on spatial conservation prioritization

The participation of private landowners in conservation is crucial to efficient biodiversity conservation. This is especially the case in settings where the share of private ownership is large and the economic costs associated with land acquisition are high. We used probit regression analysis and historical participation data to examine the likelihood of participation of Danish forest owners in a voluntary conservation program. We used the results to spatially predict the likelihood of participation of all forest owners in Denmark. We merged spatial data on the presence of forest, cadastral information on participation contracts, and individual‐level socioeconomic information about the forest owners and their households. We included predicted participation in a probability model for species survival. Uninformed and informed (included land owner characteristics) models were then incorporated into a spatial prioritization for conservation of unmanaged forests. The choice models are based on sociodemographic data on the entire population of Danish forest owners and historical data on their participation in conservation schemes. Inclusion in the model of information on private landowners’ willingness to supply land for conservation yielded at intermediate budget levels up to 30% more expected species coverage than the uninformed prioritization scheme. Our landowner‐choice model provides an example of moving toward more implementable conservation planning.     

Fragile X demonstrated retrospectively in amniotic cells cultured in low folate medium

Chromosome analysis in a boy aged 10 months, with psychomotor retardation, revealed the fragile X-chromosome in lymphocytes and skin fibroblasts cultured in low folate medium (TC 199). Amniocentesis and chromosomal analysis had been carried out during pregnancy because of advanced maternal age. Review of the slides from amniotic fluid cells grown routinely in low folate medium showed the marker X in 10.6 per cent of the metaphases. Possible explanations for the appearance of the marker X in amniotic cell culture are discussed.     

Disaccharidase deficiency in amniotic fluid from cases of cystic fibrosis

The activities of the disaccharidases maltase and sucrase in 4 amniotic fluid from cystic fibrosis (CF) pregnancies have been compared to those of 120 non CF-pregnancies. Very low levels were found in 3 of the CF-fluids. The fourth CF-fluid was normal in all measured microvillar enzyme activities. Elevated levels of disaccharidases in meconium from one of the patients born with CF, supports the idea that these enzymes are trapped in the intestinal cavity by sticky meconium.     

北极4个亚区的影响分析

在评价北极陆地生态系统影响时,人们常常强调物种和生态系统对环境变化响应的地理变化,这种变化往往与气候、生物多样性、植被带、生态系统结构和功能的南-北梯度相关联,可是,环境、生态系统的功能和结构上,以及环境史和当前气候变化的明显东-西变化显然也很重要.尽管一些地方变得温暖,但另一些地方却降温了,海洋、群岛和山脉等地理屏障的东西差异过去也对物种和植被带响应气候变化而改变分布区的能力产生了很大影响,同时,这些地理屏障为种群遗传分化和生物多样性热点区的形成提供了必要的隔离条件,这些屏障在未来气候变暖时,也将影响物种重新分布的能力.为了说明这种东西向的变化,同时也避免过分笼统或过于专业化,基于大尺度的天气和气候形成因素,北极气候影响评价项目确定了4个主要亚区.通过模拟与4个北极气候影响评价亚区有关的主要信息,导致物种分布区发生改变的地理屏障,特别是大陆的分布和海洋产生的隔离,明显会影响植被带的向北移动.对植被区向北移动的地理限制或者促进将影响将来碳的贮存和释放,以及生物圈与大气之间水和能量的交换.此外,气候变化使受威胁物种数量在各个亚区之间差别很大(白令海地区别尤其是热点),各个植被亚区重新分布的能力差异将影响每个区的生物多样性.总而言之,亚区分析表明,在整个北极地区水平上概括生态系统结构和功能的反应、物种的丧失,以及生物圈对气候系统的反馈的趋势是困难的,说明需要对北极陆地生态系统对于气候变化响应的空间变化性有深刻的认识.     

Prenatal aneuploidy detection in interphase cells by fluorescence in situ hybridization (Fish)

FISH is a quick, inexpensive, accurate, sensitive and relatively specific method for aneuploidy detection in samples of uncultured chorionic villus cells and amniotic fluid cells. FISH allows detection of the autosomal trisomies 13, 18 and 21 and X and Y abnormalities and any other chromosome abnormality for which a specific probe is available. The detection rate of these abnormalities is high in informative samples which have a concordance of > 99.5% with cytogenetic results. A relatively high number of abnormal cases are found in uninformative samples. However, such samples should be regarded as samples to be investigated further. Clinical experience with the use of FISH for prenatal diagnosis is now beyond 10,000 cases; a number of clinical protocols and smaller trials have also been carried out, resulting in 90% of attempted analyses giving informative results with a high detection rate and extraordinarily low false-positive and false-negative rates Unsolved problems remain, such as occasional technical failures, admixtures of maternal blood and up to 20% uninformative scoring results, especially for abnormal specimens. FISH is at present used as an adjunct to classical cytogenetic analysis. However, this should not be interpreted as meaning that FISH could not be used as a methodology in its own right. If FISH were to be considered a Diagnostic test then this might be the case, due to the risk of false-negative and false-positive results and the fact that FISH does not allow a diagnosis of certain structural abnormalities. If, on the other hand, FISH is considered a screening test, which means that in all abnormal (or indeterminate) cases, classical cytogenetic analysis would follow the abnormal screening test, the accuracy which is potentially higher than for other screening methods, for example in cases of trisomy 21, justifies FISH as a prenatal screening test in its own right.     

Genetic amniocentesis at 7–14 weeks of gestation

Genetic amniocentesis performed at 7–14 weeks of gestation was studied in a series of 138 patients of whom 50 wanted termination of pregnancy (⩽ 12 weeks). The material for analysis consisted of 132 samples due to two sampling failures and four samples being handled incorrectly. Forty-eight samples (36 per cent) were taken at 7–12 weeks of gestation, mainly transvaginally (36/48:75 per cent). The success rate of culture and karyotyping increased with the duration of pregnancy, but was only satisfactory from week 11 onwards. The time until harvest was then 14–15 days. The transvaginal approach is easy to perform and was accepted by the women, but we experienced bacterial or fungal overgrowth in 17 per cent of these samples, whereas no infection occurred in the samples taken transabdominally (n = 96). We conclude that genetic amniocentesis is feasible from week 11, but further studies concerning side effects, especially focusing on the procedure-related abortion risk, should be carried out before early amniocentesis is routinely applied.     

High resolution chromosomes from first trimester trophoblast cultures

A simple method for obtaining prometaphase chromosomes from cultured first trimester cells involves the addition of BrdU and FdU 11 h before harvest and ethidium bromide 1.5 h before harvest. High resolution R-banding is obtained by acridine orange staining.     

Adenosine triphosphate and adenylate energy charge in marine sediments

The determination of adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine monophosphate (AMP) concentrations in sediments from both Puget Sound and the Washington State continental shelf (USA) has been investigated. Major losses during the extraction procedure included sediment sorption, coprecipitation, and unreactivity of the soluble adenylates presumably through irreversible binding to solubilized sediment organics. Addition of phosphoric acid and the non-ionic detergent Triton X-100 to cold sulfuric acid significantly increased the extraction efficiency and sensitivity over that of sulfuric acid alone, although both methods yielded similar results when corrected for losses. Vertical profiles of ATP, total adenylates (A_T), and adenylate energy charge (EC) in sediments from both areas are presented. Profiles of A_T are similar in both areas, but EC values in the shelf sediments are significantly lower, which suggests a depressed physiological condition in the shelf population relative to that in Puget Sound.Contribution No. 513 from the University of Washington's College of Fisheries, and Contribution No. 1135 from the University of Washington's Department of Oceanography     

Comparison of transabdominal and transcervical CVS and amniocentesis: Sampling success and risk

A total of 2931 women randomized to either transabdominal CVS, transceirvical CVS, or amniocentesis were studied. Unless intended or unintended abortion had occurred, they had completed up to 28 weeks of pregnancy. No significant difference was seen between total fetal loss in the transabdominal CVS group and the amniocentesis group (6.5 and 6.8 per cent, respectively, SE difference = 0.92 per cent, p = 0.01). The total fetal loss in the transcervical CVS group was 10.1 per cent. After pooling our data with data from the Canadian randomized study and the American non-randomized study, the difference in risk between trans-cervical CVS and amniocentesis was 1.8 per cent (SE difference = 0.64 per cent, p = 0.8). When the number of failed procedures and those cases evaluated as infeasible for the assigned method-for anatomical reasons-are compared, the overall sampling efficacy is poorer transcervically than transabdominally.