Development of a field enhanced photocatalytic device for biocide of coliform bacteria

A field enhanced flow reactor using bias assisted photocatalysis was developed for bacterial disinfection in lab-synthesized and natural waters. The reactor provided complete inactivation of contaminated waters with flow rates of 50 m L/min. The device consisted of titanium dioxide nanotube arrays, with an externally applied bias of up to 6 V. Light intensity, applied voltage, background electrolytes and bacteria concentration were all found to impact the device performance. Complete inactivation of Escherichia coli W3110(~ 8 × 10~3CFU/m L) occurred in 15 sec in the reactor irradiated at 25 m W/cm~2 with an applied voltage of 4 V in a 100 ppm NaCl solution. Real world testing was conducted using source water from Emigration Creek in Salt Lake City, Utah. Disinfection of natural creek water proved more challenging, providing complete bacterial inactivation after 25 sec at 6 V. A reduction in bactericidal efficacy was attributed to the presence of inorganic and organic species, as well as the increase in robustness of natural bacteria.     

Statistical control in correlational studies: 10 essential recommendations for organizational researchers

Statistical control is widely used in correlational studies with the intent of providing more accurate estimates of relationships among variables, more conservative tests of hypotheses, or ruling out alternative explanations for empirical findings. However, the use of control variables can produce uninterpretable parameter estimates, erroneous inferences, irreplicable results, and other barriers to scientific progress. As a result, methodologists have provided a great deal of advice regarding the use of statistical control, to the point that researchers might have difficulties sifting through and prioritizing the available suggestions. We integrate and condense this literature into a set of 10 essential recommendations that are generally applicable and which, if followed, would substantially enhance the quality of published organizational research. We provide explanations, qualifications, and examples following each recommendation. Copyright © 2015 John Wiley & Sons, Ltd.     

Ozone Suppression of White Pine Net Photosynthesis

Reduction in rates of net photosynthesis following exposure to ozone was observed in eastern white pine saplings prior to the development of visible injury. Branches exposed to consecutive doses of 50–80 pphm ozone for 4 hr and 80–90 pphm ozone for 3 hr appeared to have suppressed net photosynthetic rates but results were confounded by diurnal rate variation. Photosynthetic rates of treated branches returned to normal levels following this initial ozone exposure. A second ozonation involving dosages of 90–100 pphm for 5 and 3 hr was sufficient to reduce rates of net photosynthesis by approximately 80 percent.     

ANALYSIS AND PREDICTION OF SURFACE RUNOFF IN AN URBANIZING WATERSHED USING SATELLITE IMAGERY1

ABSTRACT: This paper demonstrates how satellite image data [e.g., from Landsat 5 Thematic Mapper (TM)], in conjunction with an urban growth model and simple runoff calculations, can be used to estimate future surface runoff and, by implication, water quality within a watershed. To illustrate the method, predictions of land use change and surface runoff are shown for Spring Creek Watershed, a medium sized urbanizing watershed in Central Pennsylvania. Land cover classifications for this watershed were created from images for summertime 1986 and 1996 and subsequently used as input to the Clarke urban growth model, called SLEUTH, to predict land use changes to the year 2025. Simulations with this model show a progressive growth in the percentage of urban pixels and in impervious surface area in the watershed but also an increase in woodland, primarily in previously clear‐cut areas. Given that woodland area will continue to increase in area, surface runoff into Spring Creek is predicted to remain only slightly above present level. However, should the woodland amount fail to increase, surface runoff is then predicted to increase more significantly during the next 25 years. Finally, the concept of urban sprawl is addressed within the context of predicted increases in urbanization by relating the implied increase in impervious surface area to population density within the watershed.     

Heteroduplex formation: A potential source of genotyping error from PCR products

The formation of heteroduplexes from polymerase chain reaction (PCR) products has recently become a diagnostic tool that is routinely used for the prenatal detection of small deletions or insertions in a number of disease-causing alleles. We present evidence illustrating that heterozygous PCR products can manifest ‘invisible’ heteroduplexes that can ultimately lead to genotyping errors. Justifications for these ‘invisible’ heteroduplexes and requisite parameters to optimize their detection are presented.     

Public mosquito abatement

Mosquito abatement is a public good for which there is a long history of operating expenditures and measured performance. A simultaneous model of mosquito abundance and abatement response is developed. Parameters are estimated using time-series, cross-section data from 30 districts. Both insecticides and ditching have contributed to reduced mosquito populations. There is evidence that district managers may have invested in excess permanent control (ditching) relative to use of insecticides, and that present abatement expenditures for the average district are excessive.     

Feasibility and costs of phosphorus application limits on 39 U.S. swine operations

Concerns about manure P and water quality have prompted new regulations imposing P limits on land application of manure. Previous research established that P limits increase land needs for animal feeding operations. We evaluated the effect of N, annual P, and rotation P limits on the feasibility of manure management. A mechanistic model characterized manure management practices on 39 swine operations (20 unagitated lagoon and 19 slurry operations) in five states (Iowa, Missouri, North Carolina, Oklahoma, and Pennsylvania). Extensive information collected from each operation was used to determine effects of manure storage type, ownership structure, and application limits on attributes of manure management. Phosphorus limits had substantially greater effect on slurry operations, increasing land needs 250% (0.3 hectares per animal unit [AU]) and time for manure application 24% (2.5 min AU(-1)) for rotation P limits and 41% (4.4 min AU(-1)) for annual P limits. Annual P limits were infeasible for current land application equipment on two operations and had the greatest effect on time and costs because they required all but three slurry operations to reduce discharge rate. We recommend implementing rotation P limits (not to exceed crop N need) to minimize time effects, allow most farmers to use their current manure application methods, and allow manure to fulfill crop N and P needs in the year of application. Phosphorus limits increased potential manure value but would require slurry operations to recover at least 61% of manure value through manure sales. Phosphorus limits are likely to shape the U.S. swine industry through differential effects on the various sectors of the swine industry.     

Trace element concentrations in soil, corn leaves, and grain after cessation of biosolids applications

From 1974 to 1984, 543 Mg ha(-1) of biosolids were applied to portions of a land-reclamation site in Fulton County, IL. Soil organic C increased to 5.1% then decreased significantly (p < 0.01) to 3.8% following cessation of biosolids applications (1985-1997). Metal concentrations in amended soils (1995-1997) were not significantly different (p > 0.05) (Ni and Zn) or were significantly lower (p < 0.05) (6.4% for Cd and 8.4% for Cu) than concentrations from 1985-1987. For the same biosolids-amended fields, metal concentrations in corn (Zea mays L.) either remained the same (p > 0.05, grain Cu and Zn) or decreased (p < 0.05, grain Cd and Ni, leaf Cd, Cu, Ni, Zn) for plants grown in 1995-1997 compared with plants grown immediately following termination of biosolids applications (1985-1987). Biosolids application increased (p < 0.05) Cd and Zn concentrations in grain compared with unamended fields (0.01 to 0.10 mg kg(-1) for Cd and 23 to 28 mg kg(-1) for Zn) but had no effect (p > 0.05) on grain Ni concentrations. Biosolids reduced (p < 0.05) Cu concentration in grain compared with grain from unamended fields (1.9 to 1.5 mg kg(-1)). Biosolids increased (p < 0.05) Cd, Ni, and Zn concentrations in leaves compared with unamended fields (0.3 to 5.6 mg kg(-1) for Cd, 0.2 to 0.5 mg kg(-1) for Ni, and 32 to 87 mg kg(-1) for Zn), but had no significant effect (p > 0.05) on leaf Cu concentrations. Based on results from this field study, USEPA's Part 503 risk model overpredicted transfer of these metals from biosolids-amended soil to corn.