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431.
Despite the importance of the gills in the acquisition of food by suspension-feeding bivalve mollusks, there is almost no
information on gill organogenesis. By means of a series of stereoscan electron micrographs, this paper describes gill development
in the Chilean oyster, Ostrea chilensis, from the brooded larval stages to 1-month-old spat. A single gill rudiment was observed on each side of the mantle at a
shell length of 320 μm, and the rudiments increased in number and size until the end of the brooding period. During metamorphosis
the gill filaments increased in number from 5 or 6 to between 7 and 9. The loss of the velum and the absence of functional
gill filaments during metamorphosis are consistent with previous observations of weight loss during this critical period of
the life history, because the newly settled juvenile lacks the ability to remove particles from suspension. The end of metamorphosis
(100% of spat with dissoconch edge) was reached 36 h after larval settlement, when the gill filaments began to grow cilia,
which increased in density and differentiated as the spat developed and acquired the capability of suspension-feeding, accounting
for the increase in body weight previously recorded during this stage. The larval rudiments gave rise to the inner demibranchs.
The outer demibranchs were observed 10 days after settlement, located between the inner demibranch and the mantle. In 1-month-old
spat, the gill did not show differentiation between primary and secondary filaments, indicating that the heterorhabdic condition
characteristic of adult oysters had yet to be attained.
Received: 11 December 1998 / Accepted: 21 August 2000 相似文献
432.
The Microbial Assay for Risk Assessment (MARA) is an innovative system based on an array of 11 different microbial species freeze-dried in a 96-well micro-titre plate format. Developed for testing the toxicity of chemicals, mixtures and environmental samples, the assay employs species of a taxonomically diverse range. In addition to ten prokaryotic species, a eukaryote (yeast) is included in the range. The MARA's innate scope of a multi-dimensional test allows determination of toxicity based on a unique assay fingerprint or index, numerically expressed as the mean Microbial Toxic Concentration (MTC). The most significant potential of the test is in the additional inference that can be conveyed to the toxicity evaluation because of the presence of each of the constituent species. In view of the fact that conventional aquatic bioassays, like fish or cladoceran tests, are expensive and impractical, the MARA could provide a cost-effective solution for routine ecotoxicological testing. The performance of the MARA was evaluated to ascertain its capability and potential scope. Sensitivity to toxicants and different environmental samples was assessed. Evaluation included comparison with other tests: namely Microtox, invertebrate (Daphnia magna and Thamnocephalus platyurus) microbiotests, and respiration-inhibition and nitrification-inhibition tests. The most sensitive invertebrate test was found to be the T. platyurus microbiotest for three of the four metals tested. The LC(50) values for this test for Cd(ii), Cr(vi) and As(iii) were 0.2, 0.018 and 0.3 mg l(-1), respectively; and the corresponding most sensitive MARA species MTC values were 4.4, 2.8 and 17 mg l(-1), respectively. 相似文献
433.
434.
Impact of electrokinetic remediation on microbial communities within PCP contaminated soil 总被引:2,自引:0,他引:2
Lear G Harbottle MJ Sills G Knowles CJ Semple KT Thompson IP 《Environmental pollution (Barking, Essex : 1987)》2007,146(1):139-146
Electrokinetic techniques have been used to stimulate the removal of organic pollutants within soil, by directing contaminant migration to where remediation may be more easily achieved. The effect of this and other physical remediation techniques on the health of soil microbial communities has been poorly studied and indeed, largely ignored. This study reports the impact on soil microbial communities during the application of an electric field within ex situ laboratory soil microcosms contaminated with pentachlorophenol (PCP; 100mg kg(-1) oven dry soil). Electrokinetics reduced counts of culturable bacteria and fungi, soil microbial respiration and carbon substrate utilisation, especially close to the acidic anode where PCP accumulated (36d), perhaps exacerbated by the greater toxicity of PCP at lower soil pH. There is little doubt that a better awareness of the interactions between soil electrokinetic processes and microbial communities is key to improving the efficacy and sustainability of this remediation strategy. 相似文献
435.
Lindsey L. Thurman John E. Gross Claudia Mengelt Erik A. Beever Laura M. Thompson Gregor W. Schuurman Christopher L. Hoving Julian D. Olden 《Conservation biology》2022,36(2):e13838
Adaptive capacity (AC)—the ability of a species to cope with or accommodate climate change—is a critical determinant of species vulnerability. Using information on species’ AC in conservation planning is key to ensuring successful outcomes. We identified connections between a list of species’ attributes (e.g., traits, population metrics, and behaviors) that were recently proposed for assessing species’ AC and management actions that may enhance AC for species at risk of extinction. Management actions were identified based on evidence from the literature, a review of actions used in other climate adaptation guidance, and our collective experience in diverse fields of global-change ecology and climate adaptation. Selected management actions support the general AC pathways of persist in place or shift in space, in response to contemporary climate change. Some actions, such as genetic manipulations, can be used to directly alter the ability of species to cope with climate change, whereas other actions can indirectly enhance AC by addressing ecological or anthropogenic constraints on the expression of a species’ innate abilities to adapt. Ours is the first synthesis of potential management actions directly linked to AC. Focusing on AC attributes helps improve understanding of how and why aspects of climate are affecting organisms, as well as the mechanisms by which management interventions affect a species’ AC and climate change vulnerability. Adaptive-capacity-informed climate adaptation is needed to build connections among the causes of vulnerability, AC, and proposed management actions that can facilitate AC and reduce vulnerability in support of evolving conservation paradigms. 相似文献