Reproduction, larval growth, and reproductive development in African clawed frogs (Xenopus laevis) exposed to atrazine

Reproductive success and development of F2 offspring from F1 adult African clawed frogs (Xenopus laevis) exposed to atrazine throughout larval development and as sexually mature adults was examined. Larval X. laevis were exposed to one of four nominal concentrations of atrazine (0, 1, 10, 25 microg atrazine/l) beginning 96 hr after fertilization and continuing through two years post-metamorphosis. Clutch size and survival of offspring were used as measurement endpoints to gauge reproductive success of the F1 frogs. Larval survivorship and time to metamorphosis were used to gauge developmental success of the F2 offspring from atrazine-exposed frogs. Testes in F1 and F2 frogs were examined for incidence of anomalies, such as testicular ovarian follicles, and sex ratios in F2 offspring were investigated to determine if exposure to atrazine caused trans-generational effects (effects on F2 individuals due to exposure of F1 individuals). There were no effects of any of the studied concentrations of atrazine on clutch size of F1 frogs. There were also no effects on hatching success or time to metamorphosis. Sex ratios did not differ between F2 offspring among treatments. There was no evidence to suggest a transgenerational effect of atrazine on spawning success or reproductive development of X. laevis. This is consistent with the presence of robust populations of X. laevis in areas where they are exposed to atrazine that has been used for several decades for weed control in production of corn. Our observations also are consistent with the results of most other studies of frogs where no effects were found to be associated with exposure to atrazine. Our data do not support the hypothesis that atrazine significantly affects reproductive fitness and development of frogs.     

Plume persistence caused by back diffusion from thin clay layers in a sand aquifer following TCE source-zone hydraulic isolation

This paper concludes that back diffusion from one or a few thin clayey beds in a sand aquifer can cause contaminant persistence above MCLs in a sand aquifer long after the source zone initially causing the plume is isolated or removed. This conclusion is based on an intensive case study of a TCE contaminated site in Florida, with the processes evaluated using numerical modeling. At this site, the TCE DNAPL zone formed decades ago, and was hydraulically isolated by means of an innovative system performing groundwater extraction, treatment and re-injection. Treated water is re-injected in a row of injection wells situated a short distance downgradient of the extraction wells, creating a clean-water displacement front to efficiently flush the downgradient plume. This scheme avoids the creation of stagnation zones typical of most groundwater pump-and-treat systems, thereby minimizing the time for aquifer flushing and therefore downgradient cleanup. The system began operation in August 2002 and although the performance monitoring shows substantial declines in concentrations, detectable levels of TCE and degradation products persist downgradient of the re-injection wells, long after the TCE should have disappeared based on calculations assuming a nearly homogenous sand aquifer. Three hypotheses were assessed for this plume persistence: 1) incomplete source-zone capture, 2) DNAPL occurrence downgradient of the re-injection wells, and 3) back diffusion from one or more thin clay beds in the aquifer. After careful consideration, the first two hypotheses were eliminated, leaving back diffusion as the only plausible hypothesis, supported by detailed measurements of VOC concentrations within and near the clay beds and also by numerical model simulations that closely represent the field site hydrogeologic conditions. The model was also used to simulate a more generalized, hypothetical situation where more thin clayey beds occur in a sand aquifer with an underlying aquitard. While there is no doubt that DNAPL source mass reduction can eventually improve downgradient groundwater quality, the magnitude and time scale over which the improvement occurs is the major uncertainty given current characterization approaches. This study shows that even one thin clay bed, less than 0.2 m thick, can cause plume persistence due to back diffusion for several years or even decades after the flux from the source is completely isolated. Thin clay beds, which have a large storage capacity for dissolved and sorbed contaminant mass, are common in many types of sandy aquifers. However, without careful inspection of continuous cores and sampling, such thin clay beds, and their potential for causing long-term back-diffusion effects, can easily go unnoticed during site characterization.     

Bystander injury evaluation of children from midwestern agricultural operations

Background

With more than a million youth living on agricultural operations, it is important for parents to understand the consequences of bystander injuries that children experience in these environments. We identified the childhood injuries for bystander status and compared the severity of these injuries to the working children in the Regional Rural Injury Study-II (RRIS-II).

Methods

RRIS-II followed 16,546 children (∼ 85% of eligible) from rural communities in the Midwest for two six-month recall periods in 1999 and 2001. Demographic, injury, and exposure data were collected through comprehensive computer-assisted telephone interviews. Child injuries were cataloged using narrative scenarios into four categories: (a) directly work-related; (b) indirectly work-related; (c) non-working accomplice; and (d) non-working attendant; the latter three all being bystander categories. Poisson regression modeling was used to calculate rates of bystander injuries. Frequencies were used for comparison of severity measures.

Results

Among the 463 child injuries (aged < 20 yrs), 102 were bystander injuries. Of the bystander-related injuries, 14 were identified as indirectly work-related (working bystanders), 27 as non-working accomplice (passengers/tag-alongs), and 60 as non-working attendant (playing on the operation). The overall rate of bystander injuries was 6.4 per 1,000 people, 95% CI (5.0, 8.1). Males, compared with females, had more than twice the injury rate (8.7; 95% CI 6.4-11.8, and 3.9; 95% CI 2.7-5.7, per 1,000 people, respectively). Bystanders in this population had more severe injuries with 4% having life-threatening circumstances; of these, 4% of the accomplices and 2% of the attendants subsequently died.

Conclusions

Children who live or work on agricultural operations are vulnerable to many hazards. Therefore, this study examined child injuries and found a clear difference in the consequences of these injuries between working-related and bystanding-related injuries.

Impact on Industry

Unlike occupations such as construction and mining, where laws and organizations have been created for the protection of bystanders, agricultural bystanders have remained unprotected and have had to face the consequent injury and death outcomes. As public health professionals considering these risks, it is necessary that we work to develop more intervention studies and continue to propose suggestive guidelines for child safety in these environments so as to challenge family traditions and possibly spark public policies that will give further protection to this population.     

A survey of sub-inhibitory concentrations of antibiotics in the environment

Antibiotics are poorly metabolized, and can enter the environment via human waste streams, agricultural run-off and pharmaceutical effluent. We consequently expect to see a concentration gradient of antibiotic compounds radiating from areas of human population. Such antibiotics should be thought of as pollutants, as they can accumulate, and have biological effects. These antibiotic pollutants can increase rates of mutation and lateral transfer events, and continue to exert selection pressure even at sub-inhibitory concentrations. Here, we conducted a literature survey on environmental concentrations of antibiotics. We collated 887 data points from 40 peer-reviewed papers. We then determined whether these concentrations were biologically relevant by comparing them to their minimum selective concentrations, usually defined as between 1/4 and 1/230 of the minimum inhibitory concentration. Environmental concentrations of antibiotics surveyed often fall into this range. In general, the antibiotic concentrations recorded in aquatic and sediment samples were similar. These findings indicate that environmental concentrations of antibiotics are likely to be influencing microbial ecology, and to be driving the selection of antibiotic resistant bacteria.     

An improved dual channel PERCA instrument for atmospheric measurements of peroxy radicals

This paper describes a new dual-channel PEroxy RadiCal Amplification (PERCA) instrument, which has been designed to improve the time resolution and signal to noise and to reduce the interference caused by variations in ambient ozone concentrations. The instrument was run at the Weybourne Atmospheric Observatory (WAO), North Norfolk, during WAOWEX (Weybourne Atmospheric Observatory Winter Experiment) in January/February 2002 and INSPECTRO (Influence of clouds on the spectral actinic flux in the lower troposphere) in September 2002. The performance of the instrument is assessed and compared to that of a single channel instrument. In particular, it is shown how the precision is greatly improved in fluctuating background ozone conditions. In addition the improved time response of the instrument allows changes in peroxy radical concentrations to be related to rapid changes in nitric oxide concentrations and the ozone photolysis frequency, j(O(1)D).     

In the aftermath of the Qa'yamat:1 the Kashmir earthquake disaster in northern Pakistan

This article explores the local impact of the catastrophic earthquake in northern Pakistan on 8 October 2005. Drawing on field research, including interviews with 40 earthquake survivors, the post-disaster analysis presented here focuses upon risk awareness and the reactions of respondents to the 7.6 magnitude earthquake that devastated areas of Azad Jammu and Kashmir State, and North-West Frontier Province. The analysis provides insights into local perceptions of seismic hazard and exposure as well as survivors' priorities with regard to rebuilding and reconstruction. The article suggests that the tragedy of the devastating earthquake is entangled in a deeper knot of causal factors that are social, economic and political in nature. Rapid population growth, urbanisation, changing building styles, environmental degradation and lack of preparedness and mitigation are associated with the circumstances that place the population at risk. Remarks concerning present and future risk reduction efforts are included.     

Biodegradation of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by a Tropical Marine Bacterium, Pseudoalteromonas sp. NRRL B-30083

Pseudoalteromonas sp. NRRL B-30083 was isolated as the predominant PHBV-degrading organism from a tropical marine environment. In complex medium, the isolate grew well at temperatures between 23°C and 33°C, with an optimal doubling time of about 30 min. NaCl was required at concentrations between 0.2 N and 0.8 N. Optimal pH levels for growth were between pH 6.5 and pH 8.5. Liquid cultures grew modestly on PHBV as a sole carbon source under optimal conditions, although PHBV depolymerase activity was not detected.     

The influence of field-scale heterogeneity on the infiltration and entrapment of dense nonaqueous phase liquids in saturated formations

Numerical simulations are used for the systematic exploration of the migration and entrapment of dense nonaqueous phase liquids (DNAPLs) in heterogeneous formations. Ensembles of realizations of random, spatially correlated permeability fields are generated and employed in model simulations of a spill event. Statistical techniques are then used to quantify the sensitivity of model predictions to input parameters, thereby identifying the parameters or processes that may be of primary importance in the determination of organic liquid distributions in heterogeneous systems. Results of the study indicate that the most critical factors in modeling organic entrapment include the spill release rate, reliable estimates of the mean, variance, and vertical correlation scale of the formation permeability, and an accurate representation of the correlation between the capillary pressure–saturation function and the permeability. In contrast, the hydraulic gradient and cross-correlation of residual saturations with permeabilities are found to have only minor influence on organic liquid distributions in such heterogeneous formations.     

Inverse modeling to estimate LNAPL plume release timing

Methodologies are presented for dating releases of light nonaqueous phase liquids (LNAPLs) using an inverse modeling approach with simple analytical models. Models for LNAPL plume migration are presented to predict LNAPL plume velocity in the unsaturated and saturated zones as a function of basic soil and fluid properties. A relative mobility factor is introduced for LNAPL movement at the water table that depends primarily on the van Genuchten n parameter (related to the breadth of the soil pore size distribution) and the magnitude of water table fluctuations. Estimated LNAPL plume velocities compare reasonably with more rigorous numerical models, which may be used in cases where data availability warrant the greater effort entailed.Two methods of estimating release timing and its uncertainty are investigated. A direct estimation method is described that determines travel time for a single observed travel distance based on estimated soil and fluid properties. Release date uncertainty may be determined using the first order (FO) or Monte Carlo (MC) methods. The second method for estimating release date involves nonlinear parameter estimation utilizing distance vs. time measurements and other data.A case study is presented for a field site where independent estimates of release timing were obtained from a numerical modeling analysis. Release timing estimates based on direct inversion of the analytical timing model agree well with the numerical analysis. Results for a second field site indicate that release date confidence limits estimated by the FO method, assuming log-normally distributed travel times, are close to values determined by the MC method, which makes no assumption regarding the form of the travel time probability distribution.Results for a hypothetical problem indicate that LNAPL velocity and travel time may be accurately estimated if sufficient data on travel distance vs. time are available. Incorporating prior information on relevant soil and fluid properties into the objective function reduces the uncertainty in release date if prior estimates are accurate. However, biased prior estimates may lead to over- or underestimation of release date uncertainty. Simultaneous estimation of soil and fluid properties and release date is possible if prior information is available to condition the parameter estimates.