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41.
Volker Gloeckner Ute Hentschel Alexander V. Ereskovsky Susanne Schmitt 《Marine Biology》2013,160(4):781-791
Homoscleromorph sponges such as Oscarella spp. are characterized by unique morphological features, and Homoscleromorpha were therefore recently proposed as the fourth class of sponges. The microbiology of these sponges was mainly studied by electron microscopy while molecular studies are scarce. The aim of this study was to characterize the bacteria in Oscarella sponges using molecular tools. Denaturing gradient gel electrophoresis revealed distinct bacterial profiles in five Oscarella species and several color morphs of Oscarella lobularis. These profiles are characteristic of low microbial abundance (LMA) sponges. This was further confirmed by analysis of a 16S rRNA clone library from O. lobularis that yielded a low phylum-level diversity with dominance of Alphaproteobacteria. Bacterial communities in O. lobularis were very similar among different individuals (collected at the same site and time), five different color morphs, and specimens from different depths and locations, indicating a species-specific association. These results allow novel insights into the microbiology of the first known LMA sponge genus within the new class Homoscleromorpha. 相似文献
42.
Friederike Hoffmann Hans Røy Kristina Bayer Ute Hentschel Martin Pfannkuchen Franz Brümmer Dirk de Beer 《Marine Biology》2008,153(6):1257-1264
The Mediterranean sponge Aplysina aerophoba kept in aquaria or cultivation tanks can stop pumping for several hours or even days. To investigate changes in the chemical
microenvironments, we measured oxygen profiles over the surface and into the tissue of pumping and non-pumping A. aerophoba specimens with Clark-type oxygen microelectrodes (tip diameters 18–30 μm). Total oxygen consumption rates of whole sponges
were measured in closed chambers. These rates were used to back-calculate the oxygen distribution in a finite-element model.
Combining direct measurements with calculations of diffusive flux and modeling revealed that the tissue of non-pumping sponges
turns anoxic within 15 min, with the exception of a 1 mm surface layer where oxygen intrudes due to molecular diffusion over
the sponge surface. Molecular diffusion is the only transport mechanism for oxygen into non-pumping sponges, which allows
total oxygen consumption rates of 6–12 μmol cm−3 sponge day−1. Sponges of different sizes had similar diffusional uptake rates, which is explained by their similar surface/volume ratios.
In pumping sponges, oxygen consumption rates were between 22 and 37 μmol cm−3 sponge day−1, and the entire tissue was oxygenated. Combining different approaches of direct oxygen measurement in living sponges with
a dynamic model, we can show that tissue anoxia is a direct function of the pumping behavior. The sponge-microbe system of
A. aerophoba thus has the possibility to switch actively between aerobic and anaerobic metabolism by stopping the water flow for more
than 15 min. These periods of anoxia will greatly influence physiological variety and activity of the sponge microbes. Detailed
knowledge about the varying chemical microenvironments in sponges will help to develop protocols to cultivate sponge-associated
microbial lineages and improve our understanding of the sponge-microbe-system. 相似文献
43.
Ute Radespiel Zübeyde Sarikaya Elke Zimmermann Michael W. Bruford 《Behavioral ecology and sociobiology》2001,49(6):493-502
The grey mouse lemur (Microcebus murinus) has a dispersed social structure, within which female sleeping associations are common. These sleeping associations have been hypothesised to confer anti-predatory and thermoregulatory benefits, especially when rearing offspring. The genetic composition of these associations was determined using microsatellite markers to test predictions derived from kin selection theory. 161 (99 males, 62 females) individual M. murinus belonging to a free-living population in Ampijoroa, north-western Madagascar, were genotyped and observed over a total of 13 months distributed over the dry seasons of 3 successive years (1995-1997). Kin selection theory predicts that these female associations should consist of closely related members, and that female philopatry and male natal dispersal should characterise the dispersal pattern within this species. These predictions were confirmed by the data. Five out of six female sleeping groups consisted of one or more closely related dyads. Females that slept alone did not have close female kin in the vicinity or within the population at all. Closely related female dyads lived in significantly closer proximity than closely related male dyads and closely related male-female dyads showed intermediate proximity. In combination with the result that females possessed significantly more relatives within the population than males, these findings support the behavioural hypotheses of female philopatry and male natal dispersal. Matrilinear grouping patterns and sex-biased dispersal are therefore genetically established in a dispersed primate social organisation for the first time. The results further indicate that several generations of mouse lemurs live together within a given area, implying both an effective mechanism of kin recognition to avoid father-daughter incest and the potential for social learning to ensure individual recognition. 相似文献
44.
Spatial variability of soil gas concentration and methane oxidation capacity in landfill covers 总被引:2,自引:0,他引:2
In order to devise design criteria for biocovers intended to enhance the microbial oxidation of landfill methane it is critical to understand the factors influencing gas migration and methane oxidation in landfill cover soils. On an old municipal solid waste landfill in north-western Germany soil gas concentrations (10, 40, 90 cm depth), topsoil methane oxidation capacity and soil properties were surveyed at 40 locations along a 16 m grid. As soil properties determine gas flow patterns it was hypothesized that the variability in soil gas composition and the subsequent methanotrophic activity would correspond to the variability of soil properties. Methanotrophic activity was found to be subject to high spatial variability, with values ranging between 0.17 and 9.80 g CH4 m−2 h−1. Considering the current gas production rate of 0.03 g CH4 m−2 h−1, the oxidation capacity at all sampled locations clearly exceeded the flux to the cover, and can be regarded as an effective instrument for mitigating methane fluxes. The methane concentration in the cover showed a high spatial heterogeneity with values between 0.01 and 0.32 vol.% (10 cm depth), 22.52 vol.% (40 cm), and 36.85 vol.% (90 cm). The exposure to methane raised the oxidation capacity, suggested by a statistical correlation to an increase in methane concentration at 90 cm depth. Methane oxidation capacity was further affected by the methanotroph bacteria pH optimum and nutrient availability, and increased with decreasing pH towards neutrality, and increased with soluble ion concentration). Soil methane and carbon dioxide concentration increased with lower flow resistance of the cover, as represented by the soil properties of a reduced bulk density, increase in air capacity and in relative ground level. 相似文献
45.
SONG Yong-hui Dietfried DONNERT Ute BERG Peter G. WEIDLER Rolf NUEESCH 《环境科学学报(英文版)》2007,19(5):591-595
Seed induces and promotes the crystallization of calcium phosphate, and acts as carrier of the recovered phosphorus (P). In order to select suitable seed for P recovery from wastewater, three seeds including Apatite (AP), Juraperle (JP) and phosphate-modified Juraperle (M-JP) were tested and compared. Batch and fixed-bed column experiments of seeded crystallization of calcium phosphate were undertaken by using synthetic wastewater with 10 mg/L P phosphate. It shows that AP has bad enduring property in the crystallization process, while JP has better performance for multiple uses, and M-JP is a hopeful seed for P recovery by crystallization of calcium phosphate. 相似文献
46.
Brigitte Froneberg Ute Boldt 《International journal of occupational safety and ergonomics》2013,19(4):581-584
In order to ensure equality of occupational health care among employees of small and medium-sized enterprises (SMEs) and of larger companies, amendments in the regulations of the numerous German accident insurance funds had to be made to provide for full availability of services, as requested by German and European law. According to these amendments, sectors formerly exempted due to small size and due to lack of an adequate number of qualified personnel, had to be covered by occupational health care. In order to reach this target group new strategies of care delivery had to be developed, making use of pre-existing infrastructure and networks.In Germany, district trade association (Kreishandwerkerschaften) have proved to be very effective for introducing occupational safety and health care into SMEs by either hiring external multidisciplinary services or by establishing a common service to be used by all associated crafts establishments. In a study conducted by the Federal Office for Health and Safety at Work in 1996 (Boldt, Gille, &Grahl, 1997), 7 district trade association were looked at in detail for their strategies. The results were discussed and supplemented in a 2-day workshop. 相似文献
47.
Monika Schlegel Alessandra Baumer Mariluce Riegel Ute Wiedemann Albert Schinzel 《黑龙江环境通报》2002,22(5):418-421
An Erratum has been published for this article in Prenatal Diagnosis 22(11) 2002: 1056. We report a case of maternal isodisomy 10 combined with mosaic partial trisomy 10 (p12.31-q11.1). Chromosome examinations from a CVS sample showed a karyotype 47,XY,+mar/46,XY. The additional marker chromosome which was present in 6/25 interphase nuclei was shown by fluorescence in situ hybridization (FISH) to have been derived from a pericentromeric segment of chromosome 10. DNA analysis was performed from umbilical cord blood from the fetus after termination of the pregnancy at 18 weeks. The results showed that the two structurally normal chromosomes 10 were both of maternal origin, whereas the marker chromosome derived from the father. Autopsy of the fetus revealed hypoplasia of heart, liver, kidneys and suprarenal glands, but, apart from a right bifid ureter, no structural organ abnormalities. This fetus represents the second reported instance of a maternal uniparental disomy (UPD) 10. Copyright © 2002 John Wiley & Sons, Ltd. 相似文献
48.
Ursula Stephan Ute Strobel Philip Weller Jasmine Bachmann Renate Klaß 《Environmental Sciences Europe》1999,11(6):313-320
10 years ago, the WWF (World Wide Fund for Nature) began to support the work of environmental protection in the countries of Eastern Europe. A main topic is the Danube-Carpathian Program. With the beginning of the bombardements, connected with great amounts of chemicals released directly and as clouds from the fires and explosions, the question raises: What are the consequences for mankind and nature? Is there a risk for the Danube-Carpathian Program? The WWF feels responsible for reporting about these consequences. In July 1999, a group of two Oil Experts and two scientists (each a chemist and a toxicologist) visited Belgrad, Pan?evo and Novi Sad, took samples of soil and water, and estimated the contents of dangerous substances. The first findings and the first consequences for nature and human health are described. 相似文献
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