Noninvasive molecular genetic analysis in studying the ecology of the snow leopard: Problems and prospects

Molecular genetic methods were used to perform the species and individual identification of 117 samples of hair and feces of carnivores collected in natural habitats. The species were identified for 85.5% of the samples. The analysis of the nucleotide sequences of the cytochrome b mitochondrial gene was used to identify the samples of hair and feces of snow leopards (Uncia uncia, 40%), red foxes (Vulpes vulpes, 48%), wolves (Canis lupus, 6%), and lynx (Lynx lynx, 5%). The analysis of eight microsatellite loci allowed the genotypes of 13 snow leopards to be described. The sex of 11 animals was identified.     

Interaction of ant species in associations: An attempt to formalize behavioral characters

Behavioral adaptations of ant species were studied in associations of different biotopes in the Crimean Mountains. The pattern of an association was found to depend on the mode of the territorial behavior of dominant species, irrespective of their number. The dominance hierarchy was more strict in monodominant than in bidominant associations. Ant species demonstrated different behavioral strategies, plasticity of foraging, and avoidance of aggressive encounters. A method for formalizing interspecies relationships and a model describing stochastic properties of the biological system are proposed, which can be useful in solving various ethological and ecological problems.     

An integrated assessment of climate change impacts for Greece in the near future

Climate changes in the Mediterranean region, related to a significant increase in temperature and changes in precipitation patterns, can potentially affect local economies. Agriculture and tourism are undoubtedly the most important economic sources for Greece and these may be more strongly affected by changing future climate conditions. Climate change and their various negative impacts on human life are also detected in their environment; hence this study deals with implications, caused by changing climate, in urban and forest areas. Potential changes for the mid-twenty-first century (2021–2050) are analysed using a high-resolution regional climate model. This paper presents relevant climatic indices, indicative for potential implications which may jeopardise vital economic/environmental sectors of the country. The results provide insights into particular regions of the Greek territory that may undergo substantial impacts due to climate change. It is concluded that the duration of dry days is expected to increase in most of the studied agricultural regions. Winter precipitation generally decreases, whereas an increase in autumn precipitation is projected in most areas. Changing climate conditions associated with increased minimum temperatures (approximately 1.3°C) and decreased winter precipitation by 15% on average suggest that the risk for forest fires is intensified in the future. In urban areas, unpleasantly high temperatures during day and night will increase the feeling of discomfort in the citizens, while flash floods events are expected to occur more frequently. Another impact of climate change in urban regions is the increasing energy demand for cooling in summer. Finally, it was found that continental tourist areas of the Greek mainland will more often face heatwave episodes. In coastal regions, increased temperatures especially at night in combination with high levels of relative humidity can lead to conditions that are nothing less than uncomfortable for foreigners and the local population. In general, projected changes associated with temperature have a higher degree of confidence than those associated with precipitation.     

Ecosystem services and hydroelectricity in Central America: modelling service flows with fuzzy logic and expert knowledge

Because ecosystem services are generally not produced and used in the same place, their assessment should consider the flows of services from ecosystems to users. These flows depend on the characteristics and spatial distribution of ecosystems and users, the spatial relationships between them, and the presence of filters or barriers between ecosystems and users. The objective of this paper is to map the ecosystem services provided to the Costa Rican and Nicaraguan hydroelectric sectors, which are crucial sectors for national sustainable development and depend directly on hydrological ecosystem services. The paper presents an approach for modelling the flows of multiple services from diverse ecosystems to diverse users through different kinds of filters in a landscape. The approach uses expert knowledge and fuzzy numbers to handle uncertainties. The analyses for Costa Rica and Nicaragua show how the approach helps identify priority areas for the conservation and restoration of forests for the services they provide to the hydroelectric sector. As such, it is a useful tool for defining spatially targeted policies for the conservation of ecosystem services and for involving the users of ecosystem services in ecosystem management.     

A biology-based dynamic approach for the reconciliation of acute and chronic toxicity tests: application to Daphnia magna

There is the need to integrate existing toxicity data in a coherent framework for extending their domain of applicability as well as their extrapolation potential. This integration would also reduce time and cost-consuming aspects of these tests and reduce animal usage. In this work, based on data extracted from literature, we have assessed the advantages that a dynamic biology-toxicant fate coupled model for Daphnia magna could provide when assessing toxicity data, in particular, the possibility to obtain from short-term (acute) toxicity test long-term (chronic) toxicity values taking into account the inherent variability of D. magna populations and the multiple sources of data. The results show that this approach overcomes some of the limitations of existing toxicity tests and that the prediction errors are considerably reduced when compared with the factor from 2 to 5 obtained using acute-to-chronic ratios.     

Implications of geographic variability on Comparative Toxicity Potentials of Cu, Ni and Zn in freshwaters of Canadian ecoregions

Current methods of estimating potential environmental impacts of metals in hazard and Life Cycle Impact Assessment (LCIA) do not consider differences in chemistry and landscape properties between geographic sites. Here, we developed and applied a model for regional aquatic impact characterization of metals using an updated method for estimating environmental fate factor (FF), bioavailability factor (BF) and aquatic ecotoxicity factor (EF). We applied the model to analyze differences in Comparative Toxicity Potentials (CTPs) of Cu, Ni and Zn for 24 Canadian ecoregions. The combined impacts of regional variability in ambient chemistry (in particular DOC, pH and hardness) and landscape properties (water residence time) can change the CTPs of these metals for freshwater by up to three orders of magnitude and change the relative ranking of metal hazard between ecoregions. Variation among Canadian freshwater chemistries and landscape characteristics influence the FFs within two orders of magnitude, BFs within two orders of magnitude for Ni and Zn and four orders of magnitude for Cu, and EFs within one order of magnitude. Sensitivity of metal FFs to environmental parameters alone spans three orders of magnitude when a constant water chemistry was used for all ecoregions. These results indicate that application of regionalised metal CTPs can have a significant influence in the analysis of ecotoxicological impacts in the life cycle assessment of products and processes.     

Tree and Shrub Expansion Over the Past 34 Years at the Tree-Line Near Abisko,Sweden

Shrubs and trees are expected to expand in the sub-Arctic due to global warming. Our study was conducted in Abisko, sub-arctic Sweden. We recorded the change in coverage of shrub and tree species over a 32– to 34-year period, in three 50 × 50 m plots; in the alpine-tree-line ecotone. The cover of shrubs and trees (<3.5 cm diameter at breast height) were estimated during 2009–2010 and compared with historical documentation from 1976 to 1977. Similarly, all tree stems (≥3.5 cm) were noted and positions determined. There has been a substantial increase of cover of shrubs and trees, particularly dwarf birch (Betula nana), and mountain birch (Betula pubescens ssp. czerepanovii), and an establishment of aspen (Populus tremula). The other species willows (Salix spp.), juniper (Juniperus communis), and rowan (Sorbus aucuparia) revealed inconsistent changes among the plots. Although this study was unable to identify the causes for the change in shrubs and small trees, they are consistent with anticipated changes due to climate change and reduced herbivory.     

Changes in Tree Growth,Biomass and Vegetation Over a 13-Year Period in the Swedish Sub-Arctic

This study was conducted in the Swedish sub-Arctic, near Abisko, in order to assess the direction and scale of possible vegetation changes in the alpine–birch forest ecotone. We have re-surveyed shrub, tree and vegetation data at 549 plots grouped into 61 clusters. The plots were originally surveyed in 1997 and re-surveyed in 2010. Our study is unique for the area as we have quantitatively estimated a 19% increase in tree biomass mainly within the existing birch forest. We also found significant increases in the cover of two vegetation types—“birch forest-heath with mosses” and “meadow with low herbs”, while the cover of snowbed vegetation decreased significantly. The vegetation changes might be caused by climate, herbivory and past human impact but irrespective of the causes, the observed transition of the vegetation will have substantial effects on the mountain ecosystems.     

Viable skin efficiently absorbs and metabolizes bisphenol A

Skin contact has been hypothesized to contribute to human exposure to bisphenol A (BPA). We examined the diffusion and metabolism of BPA using viable skin models: human skin explants and short-term cultures of pig ear skin, an alternative model for the study of the fate of xenobiotics following contact exposure. ¹⁴C-BPA [50-800 nmol] was applied on the surface of skin models. Radioactivity distribution was measured in all skin compartments and in the diffusion cells of static cells diffusion systems. BPA and metabolites were further quantified by radio-HPLC. BPA was efficiently absorbed in short-term cultures, with no major difference between the models used in the study [viable pig ear skin: 65%; viable human explants: 46%; non-viable (previously frozen) pig skin: 58%]. BPA was extensively metabolized in viable systems only. Major BPA metabolites produced by the skin were BPA mono-glucuronide and BPA mono-sulfate, accounting together for 73% and 27% of the dose, in pig and human, respectively. In conclusion, experiments with viable skin models unequivocally demonstrate that BPA is readily absorbed and metabolized by the skin. The trans-dermal route is expected to contribute substantially to BPA exposure in human, when direct contact with BPA (free monomer) occurs.