Book reviews

Environment Systems and Decisions -     

Formation of polyester blends by a recombinant strain ofPseudomonas oleovorans: Different poly(3-hydroxyalkanoates) are stored in separate granules

WhenPseudomonas oleovorans (GPo1) is grown on sodium octanoate under ammonium limiting conditions, it is able to accumulate a copolyester consisting of medium chain length 3-hydroxyalkanoic acids (PHA_m). 3-Hydroxybutyrate is only incorporated in trace amounts. WhenP. oleovorans is equipped with the PHB biosynthetic genes ofAlcaligenes eutrophus (GPo1[pVK101::PP1]), it forms a polyester containing major amounts of 3-hydroxybutyrate. The resulting polymer however is a blend of PHA_m and PHB, rather than a copolymer of 3-hydroxybutyrate and medium chain length 3-hydroxyalkanoic acids [11]. To establish whether PHA_m and PHB molecules are stored in the same or separate granules by this recombinantP. oleovorans strain, we studied polymer forming cells by freeze-fracture electron microscopy. This approach is possible because previous freeze-fracture electron microscopy studies on PHA_m and PHB accumulating strains have shown that PHA_m and PHB granules can be distinguished from each other: PHA_m granules from mushroom-like structures, whereas PHB granules from needle structures during freeze-fracturing. In this paper we show that stationary phase cells of GPo1[pVK101::PP1] contained both mushroom and needle-like structures, indicating that PHA_m and PHB chains were stored in separate granules. To be able to determine whether the separation of PHA_m and PHB is complete, the respective granules were separated on sucrose gradients. A total cell extract of GPo1[pVK101::PP1] which was subjected to sucrose gradient centrifugation revealed two white bands of different densities: the upper band with a density of 1.05 g/mL consisted exclusively of PHA_m granules, while the lower band with a density of 1.19 g/mL consisted of PHB granules only. Thus, when bacteria synthesize both PHA_m and PHB, the resulting polymer chains are segregated completely and stored in separate granules.     

Evaluation of synthetic adsorbents for cleanup of herbicide-laden wastewater

Small-and large-scale farm operators and applicators urgently need user-friendly and cost-effective on-site methods to treat dilute-formulated herbicide-laden wastewater (i.e., rinsate). In this study, we investigated solvent-regenerable synthetic adsorbents, Ambersorb® 563 adsorbent, Ambersorb 572 adsorbent, and Ambersorb 575 adsorbent, as alternatives to activated carbon (Filtrasorb ® 400) for cleanup of water containing Dual 8E (a.i., metolachlor [2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-1-methylethyl)acetamide]) or Banvel 4L (a.i., dicamba [3,6-dichloro-2-methoxybenzoic acid]). Batch-type adsorption experiments were conducted to compare adsorptive capacities of the various adsorbents. Adsorption maxima, obtained from adsorption isotherms, indicated that of the Ambersorb adsorbents tested, Ambersorb 572 adsorbent generally exhibited the highest adsorptive capacities for the herbicides tested and was comparable to Filtrasorb 400. Solvent (methanol) regenerability studies demonstrated that herbicide was readily recovered from Ambersorb 572 adsorbent and Filtrasorb 400. In general, metolachlor was more readily recoverable than dicamba. In fixed-bed column studies, Ambersorb 572 adsorbent exhibited twice the adsorptive capacity of Filtrasorb 400. Fixed-bed columns were more effective in removing metolachlor from herbicide containing water regardless of the adsorbent used. Multicycling of Ambersorb 572 adsorbent resulted in 30% loss of adsorptive capacity for dicamba as Banvel 4L and no loss of adsorptive capacity for metolachlor as Dual 8E. Working capacity was reached after three adsorption-regeneration cycles. Fixed-bed columns packed with solvent-regenerable adsorbents may prove useful as part of an on-site wastewater disposal system.     

Pollution,place, and premature death: evidence from a mid-sized city

ABSTRACT

Neighborhood life expectancy varies by as much as 10 years across the City of Louisville. In 2013, the Greater Louisville Project funded by local government, businesses, and foundations, argued these differences had little to do with environmental factors. The Greater Louisville Project (2013) study argued that these neighborhood differences could be attributed 40% to socio-economic factors (with a major emphasis on education), 10% to physical environment, 30% to health behaviors, and 20% to access to medical care. To test these claims, we construct our own model of neighborhood variation in years of potential life lost (YPLL) by adding two variables testing environmental degradation. We operationalise two separate measures of environmental contamination: proximity to EPA designated brownfield sites and proximity to chemical factories in an industrial park in the neighborhood known as “Rubbertown”. We conduct several regression analyses, which show a relationship between proximity to environmental contaminants and an increase in neighborhood YPLL. Our beta weights challenge the claims made by the Greater Louisville Project, which minimize the impact nearness to environmental contaminants has on reductions in life expectancy in Louisville neighborhoods.     

Evaluating microbial purification during soil treatment of wastewater with multicomponent tracer and surrogate tests

Soil treatment of wastewater has the potential to achieve high purification efficiency, yet the understanding and predictability of purification with respect to removal of viruses and other pathogens is limited. Research has been completed to quantify the removal of virus and bacteria through the use of microbial surrogates and conservative tracers during controlled experiments with three-dimensional pilot-scale soil treatment systems in the laboratory and during the testing of full-scale systems under field conditions. The surrogates and tracers employed included two viruses (MS-2 and PRD-1 bacteriophages), one bacterium (ice-nucleating active Pseudomonas), and one conservative tracer (bromide ion). Efforts have also been made to determine the relationship between viruses and fecal coliform bacteria in soil samples below the wastewater infiltrative surface, and the correlation between Escherichia coli concentrations measured in percolating soil solution as compared with those estimated from analyses of soil solids. The results suggest episodic breakthrough of virus and bacteria during soil treatment of wastewater and a 2 to 3 log (99-99.9%) removal of virus and near complete removal of fecal coliform bacteria during unsaturated flow through 60 to 90 cm of sandy medium. Results also suggest that the fate of fecal coliform bacteria may be indicative of that of viruses in soil media near the infiltrative surface receiving wastewater effluent. Concentrations of fecal coliform in percolating soil solution may be conservatively estimated from analysis of extracted soil solids.     

Scope insensitivity in contingent valuation of complex environmental amenities

It has been argued that respondents in contingent valuation (CV) surveys, asked to value complex environmental amenities, will state willingness to pay (WTP) independently of the scope of the project. Such insensitivity to scope would be at odds with rational choice, and could therefore imply that CV is not a theoretically valid method for biodiversity valuation. The scope test in the present CV study was applied to endangered species preservation. Respondents were split in four sub-samples facing different scopes of endangered species preservation. The design allowed for both external and internal scope tests. Furthermore, the tests were split according to elicitation format. Of four external tests of insensitivity to scope, one was rejected, two gave mixed results, depending on either the type of test or elicitation format, and for the last one the null hypothesis could not be rejected. Of five internal tests, insensitivity to scope was rejected in three cases, one test gave mixed results, and one could not be rejected. Survey design features of the CV study, especially an unfamiliar sub-group of endangered species, could explain the apparent insensitivity to scope observed.     

Reproductive health in Afghanistan: results of a knowledge, attitudes and practices survey among Afghan women in Kabul

A reproductive-health knowledge, attitudes and practices (KAP) survey was carried out among 468 Afghan women of reproductive age. A convenience sample of women was selected from attendees in the outpatient departments of four health facilities in Kabul. Seventy-nine per cent of respondents had attended at least one antenatal consultation during their last pregnancy. Two-thirds (67 per cent) delivered their first child between 13 and 19 years. The Caesarean-section rate was low (1.6 per cent). Two-thirds (67 per cent) of deliveries occurred in the home. The contraceptive prevalence rate was 23 per cent (16 per cent modern and 7 per cent natural methods). Twenty-four per cent had knowledge of any STIs, although most of these women did not know correctly how to prevent them. Most of the women (93 per cent) needed authorization from their husband or a male relative before seeking professional health-care. In multivariate analysis, women's schooling was significantly associated with antenatal-care attendance (AOR 4.78), institutional delivery (AOR 2.29), skilled attendance at birth (AOR 2.07) and use of family planning (AOR 4.59). Reproductive-health indicators were noted to be poor even among these women living in Kabul, a group often considered to be the most privileged. To meet the reproductive-health needs of Afghan women, the socio-cultural aspects of their situation--especially their decision-making abilities -- will need to be addressed. A long-standing commitment from agencies and donors is required, in which the education of women should be placed as a cornerstone of the reconstruction process of Afghanistan.     

Modeling the Suitability of Potential Wetland Mitigation Sites with a Geographic Information System

Wetland mitigation is frequently required to compensate for unavoidable impacts to wetlands. Site conditions and landscape context are critical factors influencing the functions that created wetlands perform. We developed a spatial model and used a geographic information system (GIS) to identify suitable locations for wetland mitigation sites. The model used six variables to characterize site conditions: hydrology, soils, historic condition, vegetation cover, adjacent vegetation, and land use. For each variable, a set of suitability scores was developed that indicated the wetland establishment potential for different variable states. Composite suitability scores for individual points on the landscape were determined from the weighted geometric mean of suitability scores for each variable at each point. These composite scores were grouped into five classes and mapped as a wetland mitigation suitability surface with a GIS. Sites with high suitability scores were further evaluated using information on the feasibility of site modification and project cost. This modeling approach could be adapted by planners for use in identifying the suitability of locations as wetland mitigation sites at any site or region.     

Monitoring Forest Carbon Sequestration with Remote Sensing and Carbon Cycle Modeling

Sources and sinks of carbon associated with forests depend strongly on the management regime and spatial patterns in potential productivity. Satellite remote sensing can provide spatially explicit information on land cover, stand-age class, and harvesting. Carbon-cycle process models coupled to regional climate databases can provide information on potential rates of production and related rates of decomposition. The integration of remote sensing and modeling thus produces spatially explicit information on carbon storage and flux. This integrated approach was employed to compare carbon flux for the period 1992–1997 over two 165-km² areas in western Oregon. The Coast Range study area was predominately private land managed for timber production, whereas the West Cascades study area was predominantly public land that was less productive but experienced little harvesting in the 1990s. In the Coast Range area, 17% of the land base was harvested between 1991 and 2000. Much of the area was in relatively young, productive-age classes that simulations indicate are a carbon sink. Mean annual harvest removals from the Coast Range were greater than mean annual net ecosystem production. On the West Cascades study area, a relatively small proportion (< 1%) of the land was harvested and the area as a whole was accumulating carbon. The spatially and temporally explicit nature of this approach permits identification of mechanisms underlying land base carbon flux. Published online     

Whiplash-associated disorders in frontal impacts: influencing factors and consequences

This multidisciplinary in-depth investigation of accidents using 24 occupants with neck symptoms shows the complexity of whiplash-associated disorders (WAD) in frontal impacts with respect to factors that influence occurrence as well as duration of symptoms. Several different occupant kinematics have been identified, all resulting in symptoms classified as WAD. Compared to occupants restrained only by a seat belt, occupants with arm resistance influence showed a greater representation of symmetrical neck symptoms. Two cases of unbelted occupants with a neck compression mechanism far from conventional "whiplash" motion were found. Posture as well as physical and psychosocial factors such as strong negative reactions, bad prognosis expectation, and stressed daily activities influenced the duration of symptoms. Occupant characteristics and sitting posture and behavior at the time of impact are important factors when analyzing and understanding WAD.