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61.
Vera S. Domingues Vitor C. Almada Ricardo S. Santos Alberto Brito Giacomo Bernardi 《Marine Biology》2007,150(3):509-519
The genus Tripterygion (Risso 1826) is restricted to the eastern Atlantic and the Mediterranean, and comprises only three species. T. melanuros and T. tripteronotus are essentially endemic to the Mediterranean, while Tripterygion delaisi occurs in the Atlantic and in the Mediterranean. Two subspecies of T. delaisi have been described (T. d. xanthosoma in the Mediterranean and T. d. delaisi in the Atlantic). Several scenarios have been proposed for the evolution of T. delaisi subspecies, but so far its subspeciation process is not clear. In this study we present a population survey of T. delaisi including specimens from the two recognized subspecies. We combined a phylogeographic approach with estimates of the direction of migration (between the Atlantic and the Mediterranean) and of the coalescence time of the two subspecies, using polymorphic mitochondrial and nuclear genes. The results of this study clearly support the existence of two Tripterygion delaisi clades, one in the eastern Atlantic islands and another in the Atlantic coasts of Europe and in the Mediterranean. Historical migration between the islands and Western Europe plus Mediterranean was restricted, and showed a westbound trend, with a higher number of migrants going from the Western Europe plus Mediterranean into the islands. We estimated the time of coalescence of both groups of T. delaisi to be more recent than the onset of Pleistocene glaciations (1.7 Mya). Our results are consistent with previous hypothesis that consider successive dispersal events of a Tripterygion ancestor from the western African coast colonizing the Atlantic islands and the Mediterranean, promoting the evolutionary divergence between these areas. 相似文献
62.
Gustavo Muniz Dias Luiz Francisco Lembo Duarte Vera Nisaka Solferini 《Marine Biology》2006,148(4):807-815
Pelagic larvae are highly important for maintaining the gene flow among populations of sessile marine invertebrates. Colonial
ascidians consist, exclusively, of brooding species, with lecithotrophic larvae that have a limited dispersal. As a result,
there is a marked differentiation among populations. In this work, we used allozyme electrophoresis to access the genetic
variation in four populations of Symplegma rubra, a colonial ascidian frequently found in the intertidal zone of Southeastern Brazilian coast. High variability was found
at three of the four sites sampled, the exception being Praia Grande in the State of Rio de Janeiro. At this site, there was
a great preponderance of clones, which possibly reflected the enclosed nature of the location and its low water circulation
that reduce the dispersal capabilities of these animals. S. rubra did not conform to expectations for random mating (Hardy–Weinberg equilibrium): there was a deficit of heterozygotes that
was more related to the small population size than to inbreeding processes, since F
is analysis per locus revealed a deficiency of heterozygotes at only one locus—MDH*. The greatest variation in allele frequency was found for GPI-2*. Analyses of genetic variability revealed moderate differentiation among the populations (F
ST=0.051), which was unexpected for a species with a low dispersal capability. Rafting, a frequently underestimated means of
dispersal, may be the main mode of gene flow in this species over large areas, since colonies of S. rubra are frequently seen growing on drift material and there is no evidence that the larva survive for a long time in the plankton. 相似文献
63.
Vera A. Matrosova Ilya A. Volodin Elena V. Volodina Nina A. Vasilieva 《Die Naturwissenschaften》2010,97(8):707-715
The questions of individuality and stability of cues to identity in vocal signals are of considerable importance from theoretical
and conservation perspectives. While individuality in alarm calls has been reported for many sciurids, it is not well-documented
that the vocal identity encoded in the alarm calls is stable between different encounters with predators. Previous studies
of two obligate hibernating rodents, speckled ground squirrels Spermophilus suslicus, and yellow ground squirrels Spermophilus fulvus demonstrated that, after hibernation, most individuals could not be identified reliably by their alarm calls. Moreover, in
most speckled ground squirrels, individual patterns of alarm calls changed progressively over as little as 2 weeks. However,
these previous data have been obtained using the collection of alarm calls from trapped animals. Here, we examined ten free-ranging
dye-marked yellow ground squirrels to determine whether their alarm calls retain the cues to individuality between two encounters
of surrogate predators (humans), separated on average by 3 days. Discriminant function analysis showed that the alarm calls
of individual yellow ground squirrels were very similar within a recording session, providing very high individual distinctiveness.
However, in six of the ten animals, the alarm calls were unstable between recording sessions. Also, we examined ten dye-marked
individuals for consistency of acoustic characteristics of their alarm calls between the encounters of humans, differing in
techniques of call collection, from free-ranging vs trapped animals. We found differences only in two variables, both related
to sound degradation in the environment. Data are discussed in relation to hypotheses explaining the adaptive utility of acoustic
individuality in alarm calls. 相似文献
64.
Tanja Stanić Aleksandra Daković Aleksandar Živanović Magdalena Tomašević-Čanović Vera Dondur Sonja Milićević 《Environmental Chemistry Letters》2009,7(2):161-166
Adsorption of arsenic (V) by natural zeolitic tuff, modified with iron (III), was investigated. Also, the iron (III) adsorption
characteristics by natural zeolitic tuff was evaluated. It was determined that iron (III) adsorption by starting zeolitic
tuff was best represented by the Freundlich type of isotherm, having correlation coefficient (r
2) of 0.990. Arsenic (V) adsorption by iron (III)-modified zeolitic tuff followed a nonlinear type of isotherm. The best fit
of the experimental data was obtained using the Langmuir–Freundlich model (r
2 = 0.99), with the estimated maximum of arsenic (V) adsorption to iron (III)-modified zeolitic tuff of 1.55 mg/g. 相似文献
65.
Laura?AquiloniEmail author Vera?Gon?alves Alberto?F.?Inghilesi Francesca?Gherardi 《Behavioral ecology and sociobiology》2012,66(5):785-790
Crayfish are excellent model organisms to study the proximate mechanisms underlying the maintenance of dominance hierarchies
in invertebrates. Our aim here was to investigate whether Procambarus clarkii males use social eavesdropping to discriminate dominant from subordinate crayfish. To this end, we conducted an experiment
composed of a “passive” and an “active” phase. In the passive phase, “focal” individuals were allowed (treatment 1) or not
(treatment 2) to see and smell two size-matched crayfish fighting while, in the subsequent active phase, they were allowed
to freely interact with the fighting dyad. None of the recorded variables showed any significant difference between the two
treatments, but, invariably, focal individuals were able to promptly discriminate dominant from subordinate crayfish. This
study provides evidence that male crayfish recognize the social status of a conspecific without the need of direct or indirect
experience with it and avoid dominants—and thus dangerous opponents—by means of a badge of status. A form of “winner and loser
effects” could also contribute to the structuring of dominance/subordinate relationships. The implication of these results
in understanding the maintenance of dominance hierarchies in invertebrates are discussed and compared with findings previously
achieved in the context of mate choice by P. clarkii females, who do appear to use eavesdropping to identify dominants and subordinates. 相似文献
66.
Goran Gajski Marko Gerić Bojana Žegura Matjaž Novak Jana Nunić Džejla Bajrektarević Vera Garaj-Vrhovac Metka Filipič 《Environmental science and pollution research international》2016,23(15):14739-14750
Due to their increasing use, the residues of anti-neoplastic drugs have become emerging pollutants in aquatic environments. Most of them directly or indirectly interfere with the cell’s genome, which classifies them into a group of particularly dangerous compounds. The aim of the present study was to conduct a comparative in vitro toxicological characterisation of three commonly used cytostatics with different mechanisms of action (5-fluorouracil [5-FU], cisplatin [CDDP] and etoposide [ET]) towards zebrafish liver (ZFL) cell line, human hepatoma (HepG2) cells and human peripheral blood lymphocytes (HPBLs). Cytotoxicity was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and acridine orange/ethidium bromide staining. All three drugs induced time- and dose-dependent decreases in cell viability. The sensitivity of ZFL and HepG2 cells towards the cytotoxicity of 5-FU was comparable (half maximal inhibitory concentration (IC50) 5.3 to 10.4 μg/mL). ZFL cells were more sensitive towards ET- (IC50 0.4 μg/mL) and HepG2 towards CDDP- (IC50 1.4 μg/mL) induced cytotoxicity. Genotoxicity was determined by comet assay and cytokinesis block micronucleus (CBMN) assay. ZFL cells were the most sensitive, and HPBLs were the least sensitive. In ZFL cells, induction of DNA strand breaks was a more sensitive genotoxicity endpoint than micronuclei (MNi) induction; the lowest effective concentration (LOEC) for DNA strand break induction was 0.001 μg/mL for ET, 0.01 μg/mL for 5-FU and 0.1 μg/mL for CDDP. In HepG2 cells, MNi induction was a more sensitive genotoxicity endpoint. The LOEC values were 0.01 μg/mL for ET, 0.1 μg/mL for 5-FU and 1 μg/mL for CDDP. The higher sensitivity of ZFL cells to cytostatic drugs raises the question of the impact of such compounds in aquatic ecosystem. Since little is known on the effect of such drugs on aquatic organisms, our results demonstrate that ZFL cells provide a relevant and sensitive tool to screen genotoxic potential of environmental pollutant in the frame of hazard assessment. 相似文献
67.
Radić Danka S. Pavlović Vera P. Lazović Milana M. Jovičić-Petrović Jelena P. Karličić Vera M. Lalević Blažo T. Raičević Vera B. 《Environmental science and pollution research international》2017,24(27):21885-21893
Environmental Science and Pollution Research - Modern, efficient, and cost-effective approach to remediation of heavy metal-contaminated soil is based on the application of microorganisms. In this... 相似文献
68.
Bilau M De Henauw S Schroijen C Bruckers L Hond ED Koppen G Matthys C Van De Mieroop E Keune H Baeyens W Nelen V Van Larebeke N Willems JL Schoeters G 《Environment international》2009,35(1):9-13
Dioxin-like activity was measured in the serum of 1425 Flemish men and women via the CALUX assay. The adults, aged between 50 and 65 years, participated in a large biomonitoring program, executed by the Flemish Center of Expertise for Environment and Health between 2002 and 2006. Within the context of this biomonitoring program also dietary intake of dioxin-like contaminants was assessed through a food frequency questionnaire. The relation between the estimated dietary intake and the dioxin-like activity in serum was evaluated using multivariate analyses: a logistic model was performed on the total population, while a linear regression analysis was done on the subsample with quantifiable dioxin activity levels in serum. Region, gender, age, BMI, smoking status, as well as dietary habits were entered in the model, with dioxin level as an outcome estimate. Both the logistic and linear model confirmed the contribution of dietary intake to the dioxin activity measured in serum. Also BMI and region were found to be associated with dioxin activity levels. 相似文献
69.
Herman E. Wyandt Ph.D. Thomas Maker Nancy L. Fisher Shivanand R. Patil Peter Osella Frederick W. Luthardt Charles Kawada Roger Williamson Aubrey Milunsky 《黑龙江环境通报》1990,10(9):569-574
We report three cases of amniocentesis in which mosaicism for trisomy 12 was detected in two or more independent cultures. The parents elected to terminate the pregnancy in all three cases. Follow-up studies in two of the cases confirmed the mosaicism in fetal tissues (in subcutaneous tissue in one case; in fetal lung in the other), but not in blood. No fetal anomalies were evident by ultrasound or at autopsy. These results along with other reported cases demonstrate the difficulty in counselling for mosaic trisomy 12. 相似文献
70.
Cerdeira AL dos Santos NA Pessoa MC Gomes MA Lanchote VL 《Journal of environmental science and health. Part. B》2005,40(1):159-165
The region of Ribeir?o Preto City, located in Southeast of Brazil, S?o Paulo State, is an important sugarcane, soybean, and corn producing area with a high level of pesticides utilization. This region is also an important recharge area for groundwater supply of the Guarany aquifer. Since the past ten years atrazine, simazine, ametryn, tebuthiuron, diuron, 2,4-D, picloram, and hexazinone are the main herbicides used in this area. In order to study a possible leaching of some of these herbicides into the aquifer, surface, and groundwater samples were collected in a watershed during the years of 1996 to 2003, from different locations. To detect and quantify the herbicides a GC-MS (gas chromatograph/mass spectrometry) method was used. The response of the herbicides analyzed was linear over the concentration range of 0.02 to 2.0 microg/L. Analysis of groundwater revealed that the herbicides tebuthiuron, diuron, atrazine, simazine, and ametryn were not present in the samples. In the surface water collected in 1997, ametryn was present in two out of nine locations with concentrations ranging from 0.17 and 0.23 microg/L, which is above the allowable 0.1 microg/L according to the European safety level. The leaching potential of tebuthiuron, diuron, atrazine, simazine, 2,4-D, picloram, and hexazinone has been evaluated using CMLS-94, "Chemical Movement in Layered Soil," as simulation model. No leaching into the depth of the water table at 40 m was found. 相似文献