Silica nanoparticles and silver-doped silica nanoparticles induce endoplasmatic reticulum stress response and alter cytochrome P4501A activity

Engineered silica nanoparticles (SiO₂-NPs) find widespread application and may lead to exposure of humans and the environment. Here we compare the effects of SiO₂-NPs and SiO₂-NPs doped with silver (SiO₂-Ag-NPs) on survival and cellular function of human liver cells (Huh7) and Pimephales promelas (fathead minnow) fibroblast cells (FMH). In Huh7 cells we investigate effects on the endoplasmatic reticulum (ER), including ER stress, and interactions of nanoparticles (NPs) with metabolizing enzymes and efflux transporters. The NPs formed agglomerates/aggregates in cell culture media as revealed by SEM and TEM. SiO₂ and SiO₂-1% Ag-NPs were taken up into cells as demonstrated by agglomerates occurring in vesicular-like structures or freely dispersed in the cytosol. Cytotoxicity was more pronounced in Huh7 than in FMH cells, and increased with silver content in silver-doped NPs. Dissolved silver was the most significant factor for cytotoxicity. At toxic and non-cytotoxic concentrations SiO₂-NPs and SiO₂-1% Ag-NPs induced perturbations in the function of ER. In Huh7 cells NPs induced the unfolded protein response (UPR), or ER stress response, as demonstrated in induced expression of BiP and splicing of XBP1 mRNA, two selective markers of ER stress. Additionally, SiO₂-1% Ag-NPs and AgNO₃ induced reactive oxygen species. Pre-treatment of Huh7 cells with SiO₂-1% Ag-NPs followed by exposure to the inducer benzo(a)pyrene caused a significant reduced induction of CYP1A activity. NPs did not alter the activity of ABC transporters. These data demonstrate for the first time that SiO₂-NPs and SiO₂-1% Ag-NPs result in perturbations of the ER leading to the ER stress response. This represents a novel and significant cellular signalling pathway contributing to the cytotoxicity of NPs.     

Extra-pair paternity, offspring mortality and offspring sex ratio in the socially monogamous coal tit (Parus ater)

Females of many socially monogamous bird species commonly engage in extra-pair copulations. Assuming that extra-pair males are more attractive than the females’ social partners and that attractiveness has a heritable component, sex allocation theory predicts facultative overproduction of sons among extra-pair offspring (EPO) as sons benefit more than daughters from inheriting their father’s attractiveness traits. Here, we present a large-scale, three-year study on sex ratio variation in a passerine bird, the coal tit (Parus ater). Molecular sexing in combination with paternity analysis revealed no evidence for a male-bias in EPO sex ratios compared to their within-pair maternal half-siblings. Our main conclusion, therefore, is that facultative sex allocation to EPO is absent in the coal tit, in accordance with findings in several other species. Either there is no net selection for a deviation from random sex ratio variation (e.g. because extra-pair mating may serve goals different from striving for ‘attractiveness genes’) or evolutionary constraints preclude the evolution of precise maternal sex ratio adjustment. It is interesting to note that, however, we found broods without EPO as well as broods without mortality to be relatively female-biased compared to broods with EPO and mortality, respectively. We were unable to identify any environmental or parental variable to co-vary with brood sex ratios. There was no significant repeatability of sex ratios in consecutive broods of individual females that would hint at some idiosyncratic maternal sex ratio adjustment. Further research is needed to resolve the biological significance of the correlation between brood sex ratios and extra-pair paternity and mortality incidence, respectively.     

Wildlife corridors under water: an approach to preserve marine biodiversity in heavily modified water bodies

Coastal areas commonly consist of an environment of intense economic uses and are thus exposed to conflicts between anthropogenic activities and biodiversity. While several approaches of nature protection have been applied to the terrestrial domain, aquatic biotopes frequently still lack a good ecological state as required by EU policies (WFD and MSFD). For numerous years, the underwater world has been considered as one sphere and was neglected in the development of distinctive concepts of conservation for its variety of biotopes. This paper’s objective is the enhancement of ecological connectivity within the study area through the design of benthic wildlife corridors and a consequent sublittoral biotope network. A step-by-step approach is presented for the optimization of ecological potential in heavily modified coastal water bodies, using Kiel Fjord (Western Baltic Sea) as a case study. The procedure for the development of wildlife corridors includes defining and mapping of existing biotope types, the identification of key species for each biotope type and delineating their mobility range, the reconstruction of near-natural / pre-industrial conditions and deriving the protection priorities by comparing past with current / modified conditions. By harmonizing these scientific insights with the local land use of human society, proposals for biotope restoration and improvements can be made. In Kiel Fjord, compensation measures, obligatory for human interventions, such as construction work in the marine environment in this case, have been implemented and present an opportunity to enhance the connectivity of biotopes, thus creating wildlife corridors for their inhabitants. The composition of benthic wildlife corridors, forming a sublittoral biotope network in accordance with the present anthropogenic uses, holds potential for implementation in comparably altered coastal water bodies and integration into national and international frameworks, in anticipation of its functionality.     

Batterienrecycling: Möglichkeiten zur Erhöhung der Rücklaufquote

Separate collection and exploitation of used batteries facilates the recycling and eventual waste management of such heavy metals as mercury and cadmium. It is thereby possible to regain raw materials like zinc, manganese and iron for the raw material cycle. Although the collection and recycling of used batteries in Switzerland is financed by a prepaid disposal fee, their returning rate of almost 60% is too low for several reasons. A questionnaire survey carried out on 2000 households revealed the following frequenthy: People collecting paper, glass, aluminium, compost and tinplate, are more separate used batteries from ordinary garbage. The number of collecting points is supposed to be sufficient, but not all of them are sufficiently marked. The prepaid disposal fee (VEG) should become obligatory so that it would be possible to compensate the collecting points. It is not obvious from the results of the survey if the introduction of a deposit of batteries would raise the retourning rate. As far as advertising is concerned, only the “battery bag” sent to every household by the BESO seemed to influence the collecting behaviour in a positive way, poster advertising had only little effect. Appeals in newspapers, radio and television did not show any changement of the collecting behaviour. However, information and knowledge about batteries and their recycling do have a positive influence in the collecting behaviour of the consumers in this specific case.     

From farm to gun and no way back: Habsburg gunpowder production in the eighteenth century and its impact on agriculture and soil fertility

Understanding the dynamics of society’s physical exchange processes with the environment (society’s metabolism) is a major theme of long-term socioecological research. In this paper, we adapt the concept of socioecological metabolism to analyze the competition between gunpowder production and agriculture for nitrogen (N) in the pre-industrial agro-ecosystem of Pamhagen in the late eighteenth century. Saltpeter (KNO₃)—the main ingredient of gunpowder—was chemically refined from agricultural waste products, in particular manure and wood ash, which were vital for the maintenance of soil fertility. In this paper, we reconstruct nitrogen flows in the agro-ecosystem of Pamhagen and establish a nutrient balance, which allows assessing the impact of saltpeter production on agricultural soil fertility management. We find that nitrate extracted by saltpeter production in our case study was equivalent to 23 % of the total available nitrogen in manure in 1778 and 12 % in 1780. The growing demand for gunpowder and thus the artisanal production of saltpeter became influential drivers in the management of societal nitrogen flows on the local level, competing over key resources for sustaining soil fertility and leaving a substantial imprint on the nutrient budget of agricultural soils as less nitrogen was available for plant uptake.     

Assessing the safety criticality of driver behavior toward cyclists at intersections

Abstract

Objective: Detailed analyses of car-to-cyclist accidents show that drivers intending to turn right at T-junctions collide more often with cyclists crossing from the right side on the bicycle lane than drivers intending to turn left. This fact has led to numerous studies examining the behavior of drivers turning left and right. However, the most essential question still has not been sufficiently answered: is the behavior of drivers intending to turn right generally more safety critical than the behavior of those intending to turn left? The purpose of this article is to provide a method that allows to determine whether a driver’s behavior toward cyclists can retrospectively be assessed as critical or non-critical.

Methods: Several theoretical considerations enriched by findings of experimental studies were employed to devise a multi-measure method. This method was applied to a dataset containing real-world approaching behavior of 48 drivers turning right and left at four T-junctions with different sight obstructions. For each driver a behavior-specific criticality was defined based on both, their driving and gaze behavior. Moreover, based on the behavior-specific criticality of each driver, the required field of view to see a cyclist from the right was defined and was set into relation with the available field of view of the T-junction.

Results: The results show that only a small portion of the drivers within the dataset would have posed an actual risk to cyclists crossing from the right side. Those situations with a higher safety criticality did not only arise when drivers intended to turn right, but also left.

Conclusion: Therefore, the analysis can only provide an explanation for the higher proportion of accidents between drivers turning right and cyclists crossing from the right side in certain situations. Further research, for example analyses of exposure data regarding the frequency of turning manoeuvers at T-junctions, is needed in order to explain the higher proportion of accidents between drivers turning right and cyclists crossing from the right side.     

Rates of apical septal extension of Desmophyllum dianthus: effect of association with endolithic photo-autotrophs

It has been hypothesized that endolithic photo-autotrophs inside the skeleton of cold-water corals may have a mutualistic relationship with the coral host positively affecting coral calcification. This study investigated the effect of endolithic photo-autotrophs on the apical septal extension of the cold-water coral Desmophyllum dianthus at Fjord Comau, southern Chile (42.41°–42.15°S, 72.5°W). The fluorescent staining agent calcein was used to document the linear apical extension of septae for a period of one and a half years between 2006 and 2007. The results showed a severe reduction in extension rates associated with the presence of endolithic photo-autotrophs. Infested individuals grew about half as fast as non-infested polyps with a median value of 1.18 μm day^?1 compared to 2.76 μm day^?1. Contrary to the initial hypothesis, these results point toward a parasitic relationship between D. dianthus and its endolithic photo-autotrophs potentially impairing coral fitness. However, further data on physiological parameters and other aspects of the calcification process are necessary to confirm these findings.     

Distribution Pattern of Benthic Invertebrate Communities in Traunsee (Austria) in Relation to Industrial Tailings and Trophy

Traunsee, an oligotrophic Alpine lake, has suffered from inputsof industrial tailings (soda- and salt-mining industries) forseveral decades. The effects of the industrial sludges on thespatial distribution of the littoral and profundal invertebratefauna was investigated along three transects at five dates. Inthe littoral zone, no negative impacts were found. A distinctgradient in faunal composition and diversity was, however,observed along a profundal transect relative to the distance fromthe waste emission. Near the industrial input, the enhanced pH,the substrate instability, and the poor sediment quality forsubstrate- and deposit-feeders were the main factors that loweror prohibit colonization of the industrial sludges. Along atransitional zone between the waste emission and the deepestbasin, recolonization was delayed, but did occur as soon aslayers of a few mm natural sediment seal the sludge. Mobile,epibenthic organisms are the first to settle these areas, whereasrecolonization by tube-building oligochaetes and chironomidsrequires thicker sealings of the industrial sludges. Differencesin the abundance of benthic invertebrates at different profundalsites were not only related to the waste emission, but also tothe influence of the main tributary, the River Traun. Theenhanced availability of allochthonous organic matter wasprobably responsible for high densities of tubificids near theinlet in the South of Traunsee. Moreover, a higher proportion oftolerant oligochaete and ostracod species in the lower profundaloutside the influence of the industrial tailings was interpretedas reflecting the increased trophy of Traunsee in the 1970s,which forced sensitive species to shift to the upper profundalwhen the oxygen climate deteriorated.     

Applying Bayesian networks to evaluate small-scale farmers’ perceptions of native reforestation practices in Brazil’s Caatinga biome

In Brazil’s semi-arid Northeast, most rural dwellers derive income from the dry Caatinga forest through livestock farming, fruit collection, and firewood extraction. However, recurring droughts and inadequate land use practices jeopardize farmers’ livelihoods. The drought-resistant, endemic Umbuzeiro tree provides fruit for direct consumption and allows for the creation of transformed products. The planting of this native species can enhance the well-being of the ecosystem and establish future benefits for smallholdings. However, it is crucial that when taking up innovative practices to cope with environmental change, a willingness to apply them should be fostered among local farmers. We used constellation analysis as a transdisciplinary approach to identify elements of current land management which subsequently defined the nodes of a Bayesian network (BN). We developed probabilities of practice uptake that strengthen success, namely the conservation of natural resources while securing the incomes of smallholders. In collaboration with stakeholders and experts, 25 identified nodes for the BN were tested under various scenarios. Adopting all suggested innovative practices secures the final objectives—ecosystem health and farmer benefits (approx. 90%). The analysis quantified the relevance of single issues that may impede farmers to adopt the practices, such as having to cultivate seedlings or avoiding long-term investments. Further crucial actions include the fencing-off of livestock and marketing pathways. Affordable credit, research, and supportive farmers’ institutions can encourage farmers to implement innovative practices. The use of modeled scenarios can provide evidence, which might encourage sustainable land management.