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211.
M. Victoria L. F. L. Tort M. García A. Lizasoain L. Maya J. P. G. Leite M. P. Miagostovich J. Cristina R. Colina 《Food and environmental virology》2014,6(2):116-124
The aim of this study was to assess the viral contamination of group A rotavirus (RVA), norovirus (NoV), and human astrovirus (HAstV) in sewage directly discharged into Uruguay River and to characterize RVA genotypes circulating in Uruguay. For this purpose, sewage samples (n = 96) were collected biweekly from March 2011 to February 2012 in four Uruguayan cities: Bella Unión, Salto, Paysandú, and Fray Bentos. Each sample was concentrated by ultracentrifugation method. Qualitative and quantitative RT-PCR for RVA, NoV, and HAstV were performed. A wide dissemination of gastroenteric viruses was observed in the sewage samples analyzed with 80 % of positivity, being NoV (51 %) the most frequently detected followed by RVA with a frequency of 49 % and HAstV with 45 %. Genotypes of RVA were typed using multiplex semi-nested RT-PCR as follows: P[8] (n = 15), P[4] (n = 8), P[10] (n = 1), P[11] (n = 1), G2 (n = 29), and G3 (n = 2). The viral load ranged from 103 to 107 genomic copies/liter, and they were detected roughly with the same frequency in all participant cities. A peak of RVA and HAstV detection was observed in colder months (June to September), whereas no seasonality was observed for NoV. This study demonstrates for the first time, the high degree of gastroenteric viral contamination in the country; highlighting the importance of developing these analyses as a tool to determine the viral contamination in this hydrographic boundary region used by the local populations for recreation and consumption, establishing an elevated risk of gastroenteric diseases for human health. 相似文献
212.
Caste-specific pheromone biosynthesis is a prerequisite for reproductive skew in the honeybee. Nonetheless, this process is
not hardwired but plastic, in that egg-laying workers produce a queen-like pheromone. Studies with Dufour’s gland pheromone
revealed that, in vivo, workers’ gland biosynthesis matches the social status of the worker, i.e., sterile workers showed
a worker-like pattern whereas fertile workers showed a queen-like pattern (production of the queen-specific esters). However,
when incubated in vitro, the gland spontaneously exhibits the queen-like pattern, irrespective of its original worker type,
prompting the notion that ester production in workers is under inhibitory control that is queen-dependent. We tested this
hypothesis by exposing queen or worker Dufour’s glands in vitro to brain extracts of queens, queenright (sterile) workers
and males. Unexpectedly, worker brain extracts activated the queen-like esters biosynthesis in workers’ Dufour’s gland. This
stimulation was gender-specific; queen or worker brains demonstrated a stimulatory activity, but male brains did not. Queen
gland could not be further stimulated. Bioassays with heated and filtered extracts indicate that the stimulatory brain factor
is below 3,000 Da. We suggest that pheromone production in Dufour’s gland is under dual, negative–positive control. Under
queenright conditions, the inhibitor is released and blocks ester biosynthesis, whereas under queenless conditions, the activator
is released, activating ester biosynthesis in the gland. This is consistent with the hypothesis that queenright workers are
unequivocally recognized as non-fertile, whereas queenless workers try to become “false queens” as part of the reproductive
competition. 相似文献
213.
Fassiano Anabella Victoria March Hugo Santos Marina Juárez Ángela Beatriz Ríos de Molina María del Carmen 《Environmental science and pollution research international》2022,29(21):31384-31399
Environmental Science and Pollution Research - Imazethapyr, a selective systemic herbicide, is widely used in agriculture and it is frequently detected in water bodies close to application areas.... 相似文献
214.
215.
Elissa M. Olimpi Hallie Daly Karina Garcia Victoria M. Glynn David J. Gonthier Claire Kremen Leithen K. M'Gonigle Daniel S. Karp 《Conservation biology》2022,36(4):e13902
Farmland diversification practices (i.e., methods used to produce food sustainably by enhancing biodiversity in cropping systems) are sometimes considered beneficial to both agriculture and biodiversity, but most studies of these practices rely on species richness, diversity, or abundance as a proxy for habitat quality. Biodiversity assessments may miss early clues that populations are imperiled when species presence does not imply persistence. Physiological stress indicators may help identify low-quality habitats before population declines occur. We explored how avian stress indicators respond to on-farm management practices and surrounding seminatural area (1-km radius) across 21 California strawberry farms. We examined whether commonly used biodiversity metrics correlate with stress responses in wild birds. We used ∼1000 blood and feather samples and body mass and wing chord measurements, mostly from passerines, to test the effects of diversification practices on four physiological stress indicators: heterophil to lymphocyte ratios (H:L), body condition, hematocrit values, and feather growth rates of individual birds. We then tested the relationship between physiological stress indicators and species richness, abundance, occurrence, and diversity derived from 285 bird point count surveys. After accounting for other biological drivers, landscape context mediated the effect of local farm management on H:L and body condition. Local diversification practices were associated with reduced individual stress in intensive agricultural landscapes but increased it in landscapes surrounded by relatively more seminatural area. Feathers grew more slowly in landscapes dominated by strawberry production, suggesting that nutritional condition was lower here than in landscapes with more crop types and seminatural areas. We found scant evidence that species richness, abundance, occurrence, or diversity metrics were correlated with the individual's physiological stress, suggesting that reliance on these metrics may obscure the impacts of management on species persistence. Our findings underscore the importance of considering landscape context when designing local management strategies to promote wildlife conservation. 相似文献