Genetic engineering for practical application

Genetic engineering has ushered in a new era in biology. Although many problems are still to be solved, there are examples that point to a possible later application for the benefit of mankind: Bacteria can be manipulated to degrade crude-oil spillages, to produce human insulin and to bind nitrogen from the air. If all the bacteria that are indigenous to agricultural soils could be made to bind nitrogen, an increase in soil fertility might well result.     

Operating characteristics of a fabric filter on a peaking/cycling boiler without auxiliary preheat or reheat

A fabric filter has been on-line for one year on a coal-fired boiler that is primarily a peaking unit within the power schedule. For the first six months, Eastern Kentucky coal with 2.5% sulfur was the fuel source for 30% of the time, with low-sulfur Montana coal constituting the remaining fuel during the operation period. Bag life has been excellent with no bag failures reported to date, and the pressure drops have been low. There has never been an auxilliary heat source to preheat the fabric filter for start-up, nor to reheat the fabric filter when operating at reduced load with associated low back-end temperatures. Conclusions are that the filter cake formed does protect the bags from blinding at low load conditions, and a special acid-resistant finish applied to the glass fibers protects the bags when high-sulfur coal is burned at low temperatures. Overall, this installation provides an excellent data base for cycling service and high-sulfur coal usage with a fabric filter.     

Food-exchange by foragers in the hive – a means of communication among honey bees?

Dancing and trophallactic behaviour of forager honey bees, Apis mellifera ligustica >Spinola, that returned from an automatic feeder with a regulated flow rate of 50% weight-to-weight sucrose solution (range: 0.76–7.65 μl/min) were studied in an observation hive. Behavioural parameters of dancing, such as probability, duration and dance tempo, increased with the nectar flow rate, though with very different response curves among bees. For trophallaxis (i.e. mouth-to-mouth exchange of food), the frequency of giving-contacts and the transfer rate of the nectar increased with the nectar flow rate. After unloading, foragers often approached other nest mates and begged for food before returning to the food source. This behaviour was less frequent at higher nectar flow rates. These results show that the profitability of a food source in terms of nectar flow rate had a quantitative representation in the hive through quantitative changes in trophallactic and dancing behaviour. The role of trophallaxis as a communication channel during recruitment is discussed. Received: 14 January 1995/Accepted after revision: 14 August 1995     

Electromagnetic fields: principles of exposure mitigation.

Basic principles of reducing exposure to electromagnetic fields are reviewed in this article. Measures to reduce exposure can be divided into organisational/administrative and technical/engineering actions. Both strategies are briefly analysed and the basic principles of the theory of shielding are presented. A definition of shielding effectiveness (SE) is given, and the results from the general Transmission Lines Theory are presented. Practical situations of shielding static and time-varying electric and magnetic fields are discussed on the basis of the physical properties of the fields and of the shield.     

Determination of Microbial Activity in Marine Sediments by Resazurin Reduction

A simple method is presented to evaluate microbial activity in aquatic sediments. The method is based on resazurin reduction by microbial electron transport chains and reduced chemical compounds present in the sample. The addition of m-cresol, which inhibits enzyme activity, allows one to measure microbial metabolism by difference. Small aliquots of sediment (about 1 g FW) are incubated at 20°C with resazurin solution, with and without m-cresol. The sample is then filtered and the unreduced resazurin is measured at 600 nm.

Testing the method with a bacterial suspension gave a resazurin reduction of 89 μg/h/10⁹ cells. In a few marine coastal sediment samples, the resazurin reduction in aerobic conditions was in the range of 0.31 to 219.3 μg/h/g DW, which is equivalent to an oxygen consumption of 0.02 to 15.32 μg/h/g DW.