Comparative genomics: methods and applications

Interpreting the functional content of a given genomic sequence is one of the central challenges of biology today. Perhaps the most promising approach to this problem is based on the comparative method of classic biology in the modern guise of sequence comparison. For instance, protein-coding regions tend to be conserved between species. Hence, a simple method for distinguishing a functional exon from the chance absence of stop codons is to investigate its homologue from closely related species. Predicting regulatory elements is even more difficult than exon prediction, but again, comparisons pinpointing conserved sequence motifs upstream of translation start sites are helping to unravel gene regulatory networks. In addition to interspecific studies, intraspecific sequence comparison yields insights into the evolutionary forces that have acted on a species in the past. Of particular interest here is the identification of selection events such as selective sweeps. Both intra- and interspecific sequence comparisons are based on a variety of computational methods, including alignment, phylogenetic reconstruction, and coalescent theory. This article surveys the biology and the central computational ideas applied in recent comparative genomics projects. We argue that the most fruitful method of understanding the functional content of genomes is to study them in the context of related genomic sequences. In particular, such a study may reveal selection, a fundamental pointer to biological relevance.     

Epicuticular wax of large and small white butterflies,<Emphasis Type="Italic"> Pieris brassicae</Emphasis> and<Emphasis Type="Italic"> P. rapae crucivora</Emphasis>: qualitative and quantitative comparison between diapause and non-diapause pupae

We compared the quantity and quality of the epicuticular wax of diapause and non-diapause pupae in two closely related Pieris species, P. brassicae and P. rapae crucivora. Main components of their epicuticular wax were identified as hydrocarbons. In P. brassicae, more than 95% of hydrocarbons were saturated regardless of whether the pupae were in diapause or not. In P. rapae crucivora, 93% of hydrocarbons were saturated in non-diapause pupae whereas in diapause pupae 41% were saturated and 59% unsaturated. From measurements of body surface area by nuclear magnetic resonance microimaging, we calculated the average thickness of the wax layer. The thickness in diapause and non-diapause pupae of P. brassicae was 800 and 160 nm, respectively. In P. rapae crucivora, the thickness was 195 nm in diapause and 11 nm in non-diapause. This is the first report to clarify the compositional difference in epicuticular wax between diapause and non-diapause pupae.     

利用聚磷菌快速内源性反硝化脱氮研究

从部分聚磷菌在缺氧状态下的反硝化吸磷现象出发，探索了利用经过厌氧释磷，菌体内含有大量PHB的聚磷菌进行快速内源性反硝化脱氮的可能性。试验结果表明：聚磷菌在厌氧段充分有效释磷后，在缺氧段初期3—5min内，就能将30—40mg／L的NO^-3；内源性反硝化脱氮去除65％—75％，达到一级排放标准。     

Closely associated theropod trackways from the Jurassic of Zimbabwe

Eighty-eight tracks of large theropod dinosaurs were found in the mid-Jurassic of Zimbabwe. Among the tracks, at least five adjacent trackways are recorded. The adjacent tracks were probably made by animals traveling as a group, given that they are in relatively close succession; that there are three overlapping tracks (among just 23) suggesting reasonably close associations of the animals; that all the tracks are apparently of the same ichnotaxon; that the preservational types of the tracks are similar; and that the tracks are all of animals traveling in one general direction closely associated in time (there are no returning tracks of the same animals or of those of other species; presence of such tracks would be highly probable if the tracks were made over a period of time of even several hours). Nearby, recently discovered giant sauropod tracks, the first in sub-Saharan Africa, indicate a realistic potential of predator/prey interactions between the two groups of dinosaurs.     

Mating success and fidelity to territories in a fallow deer lek: a female removal experiment

A lek is a clump of male territories that females visit only for mating; it is quite a rare mating system among mammals. As we wanted to investigate the role of past male mating success on their fidelity to lek territories, we displaced fallow deer females by a lek (Lek A), i.e. we removed the resources for rutting males. They moved to a new area followed by some of the territorial males (mobile males) where they established a new lek (Lek B). Other males (faithful males) stayed on Lek A. The former had lower mating success and dominance ranking in the early, undisturbed part of the rut. After disturbance, the MS of faithful males was much reduced, while that of mobile males was higher on Lek B than it had been on Lek A. We then disturbed Lek B. Females returned to Lek A, and the mating success of faithful males reverted to its original level. Past mating success was the main factor predicting whether a male was faithful or mobile. We illustrated for the first time the importance of past experience in promoting lek fidelity; this experiment gives an insight into the mechanisms leading to the traditional use of leks.     

广州市水政水资源管理信息系统开发研究

广州市水政水资源管理信息系统是一个基于GIS技术的、客户／服务器结构下的环境信息系统，本文介绍了系统的总体设计与关键技术，该系统是为水资源主管部门进行水资源日常管理而设计的，目的是为了提高水资源管理的科学化，现代化水平，系统开发的关键技术要有服务器数据库建设、客户端与服务器的数据连接以及组件式GIS（MapObjects）的应用。     

Predatory behavior in a necrophagous bee<Emphasis Type="Italic"> Trigona hypogea</Emphasis> (Hymenoptera; Apidae,Meliponini)

Although most bees feed on nectar and pollen, several exceptions have been reported. The strangest of all is the habit found in some neotropical stingless bees, which have completely replaced pollen-eating by eating animal protein from corpses. For more than 20 years, it was believed that carrion was the only protein source for these bees. We report that these bees feed not only off dead animals, but on the living brood of social wasps and possibly other similar sources. Using well developed prey location and foraging behaviors, necrophagous bees discover recently abandoned wasps nests and, within a few hours, prey upon all immatures found there.     

European Atlantic: the hottest oil spill hotspot worldwide

Oil spills caused by maritime transport of petroleum products are still an important source of ocean pollution, especially in main production areas and along major transport routes. We here provide a historical and geographic analysis of the major oil spills (>700 t) since 1960. Spills were recorded from several key marine ecosystems and marine biodiversity hotspots. The past four decades have been characterized by an overall decrease in the number of accidents and tonnes of oil spilled in the sea, but this trend was less distinct in the European Atlantic area. Recent black tides from the Erika and Prestige vessels provided new evidence for the high risk of accidents with serious ecological impact in this area, which according to our analysis is historically the most important oil spill hotspot worldwide. The English Channel and waters around Galicia in Spain were the areas with most accidents. Maritime transport in European Atlantic waters has been predicted to continue increasing. Together with our own results this suggests that, in addition to measures for increased traffic safety, deployment of emergency capacities in the spill hotspot areas may be crucial for a sustainable conservation of sea resources and ecosystems.Electronic Supplementary Material  Supplementary material is available for this article if you access the article at . A link in the frame on the left on that page takes you directly to the supplementary material.     

A new C12 alcohol identified as a sex pheromone and a trail-following pheromone in termites: the diene (<Emphasis Type="Italic">Z</Emphasis>,<Emphasis Type="Italic">Z</Emphasis>)-dodeca-3,6-dien-1-ol

The diunsaturated C12 alcohol (Z,Z)-dodeca-3,6-dien-1-ol (dodecadienol) has been characterized by GC-MS and FTIR as a novel releaser pheromone in termites. This alcohol identified in Ancistrotermes pakistanicus (Termitidae, Macrotermitinae) possesses a double pheromonal function which again illustrates the chemical parsimony of termites compared with other social insects. In workers, dodecadienol elicits trail-following at a very low concentration (activity threshold at 0.1 pg/cm of trail); in male alates it induces trail-following at a low concentration (1–10 pg/cm) and sexual attraction at a higher concentration (about 1 ng). Traces of the monounsaturated C12 alcohol (Z)-dodec-3-en-1-ol (dodecenol), known as a trail pheromone of several Macrotermitinae, were also found in the sternal gland extracts of A. pakistanicus, although only dodecadienol was present at the surface of the sternal gland. Workers of A. pakistanicus are not sensitive to dodecenol, but they are as sensitive to dodecatrienol as to dodecadienol. However, in the study area (Vietnam), A. pakistanicus is living in sympatry only with those Macrotermitinae using dodecenol as a trail pheromone, the foraging populations therefore being well isolated through their respective trail pheromones. The presence of three types of unsaturated C12 alcohols as releaser pheromones in the only Macrotermitinae subfamily is discussed, and a possible biosynthetic pathway from linoleic acid is proposed for dodecadienol.     

径向基函数网络在水环境质量评价分区中的应用

为探讨水环境质量综合评价的客观方法 ,以江西抚州地下潜水水质资料为例 ,建立了该区地下潜水水环境质量综合评价的RBF ANN网络模型。通过与其它方法对比 ,结果表明 ,利用用RBF ANN方法进行水环境质量评价不仅方法简便 ,而且结论更接近客观实际