To understand the influence patterns and interactions of three important environmental factors, i.e. soil water content, oxygen
concentration, and ammonium addition, on methane oxidation, the soils from landfill cover layers were incubated under full
factorial parameter settings. In addition to the methane oxidation rate, the quantities and community structures of methanotrophs
were analyzed to determine the methane oxidation capacity of the soils. Canonical correspondence analysis was utilized to
distinguish the important impact factors. Water content was found to be the most important factor influencing the methane
oxidation rate and Type II methanotrophs, and the optimum value was 15% (w/w), which induced methane oxidation rates 10- and
6- times greater than those observed at 5% (w/w) and 20% (w/w), respectively. Ambient oxygen conditions were more suitable
for methane oxidation than 3% oxygen. The addition of 100 mg-N·kgdrysoil−1 of ammonium induced different effects on methane oxidation capacity when conducted at low or high water content. With regard
to the methanotrophs, Type II was sensitive to the changes of water content, while Type I was influenced by oxygen content.
Furthermore, the methanotrophic acidophile, Verrucomicrobia, was detected in soils with a pH of 4.9, which extended their known living environments. 相似文献
Microbial communities are important for high composting efficiency and good quality composts. This study was conducted to compare the changes of physicochemical and bacterial characteristics in composting from different raw materials, including chicken manure (CM), duck manure (DM), sheep manure (SM), food waste (FW), and vegetable waste (VW). The role and interactions of core bacteria and their contribution to maturity in diverse composts were analyzed by advanced bioinformatics methods combined sequencing with co-occurrence network and structural equation modeling (SEM). Results indicated that there were obviously different bacterial composition and diversity in composting from diverse sources. FW had a low pH and different physiochemical characteristics compared to other composts but they all achieved similar maturity products. Redundancy analysis suggested total organic carbon, phosphorus, and temperature governed the composition of microbial species but key factors were different in diverse composts. Network analysis showed completely different interactions of core bacterial community from diverse composts but Thermobifida was the ubiquitous core bacteria in composting bacterial network. Sphaerobacter and Lactobacillus as core genus were presented in the starting mesophilic and thermophilic phases of composting from manure (CM, DM, SM) and municipal solid waste (FW, VW), respectively. SEM indicated core bacteria had the positive, direct, and the biggest (>?80%) effects on composting maturity. Therefore, this study presents theoretical basis to identify and enhance the core bacteria for improving full-scale composting efficiency facing more and more organic wastes.