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31.
The epiphytic invertebrates found on Potamogetonperfoliatus L. in Traunsee, an oligotrophic Alpine lake inAustria, were investigated in August and October 1998 in orderto study the impact of industrial tailings discharged into thelake. 113 taxa were found, 54 could be identified to thespecies level. Their total abundance varied between ca.190,000 and 1,138,000 ind. m-2 lake bottom area. Thisepiphytic assemblage was dominated by Dreissenapolymorpha and Sida crystallina, which resulted in avery low overall species diversity. Multivariate statisticalanalyses revealed significant differences in the communitystructure between three sites, each of them was located at adifferent distance from the site of industrial waste emission.These differences were interpreted as variations which reflectthe patchiness within highly structured habitats rather thanas being the result of the industrial pollution.  相似文献   
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Traunsee, an oligotrophic Alpine lake, has suffered from inputsof industrial tailings (soda- and salt-mining industries) forseveral decades. The effects of the industrial sludges on thespatial distribution of the littoral and profundal invertebratefauna was investigated along three transects at five dates. Inthe littoral zone, no negative impacts were found. A distinctgradient in faunal composition and diversity was, however,observed along a profundal transect relative to the distance fromthe waste emission. Near the industrial input, the enhanced pH,the substrate instability, and the poor sediment quality forsubstrate- and deposit-feeders were the main factors that loweror prohibit colonization of the industrial sludges. Along atransitional zone between the waste emission and the deepestbasin, recolonization was delayed, but did occur as soon aslayers of a few mm natural sediment seal the sludge. Mobile,epibenthic organisms are the first to settle these areas, whereasrecolonization by tube-building oligochaetes and chironomidsrequires thicker sealings of the industrial sludges. Differencesin the abundance of benthic invertebrates at different profundalsites were not only related to the waste emission, but also tothe influence of the main tributary, the River Traun. Theenhanced availability of allochthonous organic matter wasprobably responsible for high densities of tubificids near theinlet in the South of Traunsee. Moreover, a higher proportion oftolerant oligochaete and ostracod species in the lower profundaloutside the influence of the industrial tailings was interpretedas reflecting the increased trophy of Traunsee in the 1970s,which forced sensitive species to shift to the upper profundalwhen the oxygen climate deteriorated.  相似文献   
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Trypsin is a prototype of a large group of enzymes belonging to serine proteinases. The X-ray crystal-structure analyses of its proenzyme trypsinogen, of the active trypsin and of their complexes formed with the pancreatic trypsin inhibitor (PTI) have considerably enhanced our understanding of the mechanisms of activitation, action and inhibition. The trypsinogen is an incompletely folded molecule. Its substrate-binding site becomes only completely fixed upon the enzymatic cleavage of an N-terminal peptide. The contact regions of trypsin and PTI are almost complementary. The complex formed is a (stable) intermediate in the normal tryptic substrate-cleavage reaction.  相似文献   
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Objective: To evaluate health impact of boron in drinking water. Methods: A regional scale geographical study in Northern France was conducted. Assessment of boron blood levels in a group of 180 healthy individuals and correlation with boron content in drinking water were followed by an assessment of health indicators such as birth rates, mortality rates, and sex ratios in zones of different boron content in drinking water. Results: After necessary adjustments, men living in municipalities with more than 0.30 mg/L of boron in drinking water had elevated but not significant boron blood levels compared with those living in municipalities with boron water levels of less than 0.30 mg/L (159.1 versus 123.0 ng/g; p > 0.05). The standardized birth ratio adjusted for the reference geographic zone and calendar time period was 1.07 and 1.28 in the low and high (>0.3 mg/L) boron content municipalities, respectively. The birth rate in municipalities with high boron content in drinking water was higher than that of the reference geographic zone and of the French general population (p < 10-4). The standardized mortality ratio adjusted for the reference geographic zone and calendar time period was 0.94 and 0.92 in the low and high boron content municipalities, respectively. The mortality rate in municipalities with high boron content in drinking water was less than that of the reference geographic zone and of the French general population (p < 10-3). No statistical difference was noted in the male–female sex ratios between the different municipality zones (p = 0.45). Conclusions: The results of this study do not support the idea of a deleterious effect of boron on human health, at the boron water level contents found in this specific region. In fact, there is a tendency toward a beneficial effect with low-dose environmental exposure (less than 1 mg/L of boron) in drinking water.  相似文献   
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In vitro effects of Pb2+, the pyrethroid insecticides cypermethrin, fenvalerate and the syner‐gist piperonyl butoxide on sodium‐potassium‐activated adenosine triphosphatase (Na,K‐ATPase) from dog kidney were determined. Pb2+ with an estimated IC50 value of 5.2 μM was found to be a potent inhibitor of Na,K‐ATPase activity, whereas Na,K‐ATPase was less sensitive to the pyrethroids tested and piperonyl butoxide. Investigation with circular dichroism (CD) spec‐troscopy showed that inhibition occurs through conformational changes of the α‐subunit of the enzyme. The kinetic characteristics of inhibition of Na,K‐ATPase with varying substrate (ATP) concentrations as well as with varying Na+ concentrations exhibited a competitive type of inhibition with Pb2+ in the μM range. With Pb2+ alone in the enzyme assay no conformational changes of the protein could be observed which confirmed the assumption that Pb2+ can bind to the Na+ binding site of the α‐subunit. Uncompetitive type of inhibition occurred with varied K+ concentrations demonstrating that this cation binding site is not affected directly by Pb2+.

Complete reversal of Pb2+ by DTT confirms that a possible target for interaction of this heavy metal ion with Na, K‐ATPase are specific SH groups.

Synergistic effects could only be determined with higher Pb2+ concentrations of 3, 5 and 7 μM plus piperonyl butoxide while all other combinations with this heavy metal plus organic substances where of the additive type. With CD spectroscopy also only additive effects were observed. These results demonstrate that higher concentrations of piperonyl butoxide favor the binding of Pb2+ to the Na+ binding site by conformational changes of the protein.  相似文献   
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Aim and Background

The supply and use of energy is related to environmental impacts, which cause significant economic damage. As these costs are not reflected in the price of energy, there is little incentive for the polluter to reduce the pressure on the environment. From an economic point of view, environmental resources and services are used beyond the optimal level. The quantification of external costs has been an area of intensive research, in particular within the series of ExternE projects funded by the European Commission. Although external cost estimates have been successfully used to support European environmental legislation, the assessment of external costs is still a matter of significant uncertainties — in particular in areas were potential large environmental impacts are expected. In spite of uncertainty and limited knowledge, policy needs to require guidelines for the evaluation of energy and environmental policy measures.

Main Features

Based on a critical review of the current literature, recommendations for the quantification of external costs from renewable electricity generation in comparison to fossil nuclear technologies are derived.

Results and Discussion

Current electricity market prices do not reflect the total costs of electricity generation. Quantifiable external costs from fossil electricity generation are in the same order as private generation costs. The internalisation of external costs will improve the competitiveness of renewable energy technologies. To avoid market distortion, policy shall implement framing conditions supporting the further internalisation of external costs.

Conclusions

Costs for supporting renewable energy via the German feed-in tariffs are compensated for by external costs avoided.

Perspectives

Fossil and nuclear energies are more expensive than is teflected by economical quantification. In contrast, the costs for renewable energies tell the truth even today. The sooner the external costs are integrated in the pricing, the sooner the relaunch of energy supply will attract interest, also from the economic point of view.  相似文献   
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