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821.
固相微萃取(SPME)技术在水质监测中的应用 总被引:3,自引:0,他引:3
固相微萃取技术(SPME)作为一种样品前处理技术,具有方便、快捷、不使用有机溶剂、灵敏度高、价格低廉等优点,已被广泛地应用于环境样品的分析.本文综合评述了采用SPME法预处理环境水体中的有机物、无机离子等污染物的监测情况,并对SPME法在环境水质监测中的应用以及对国外的研究进展进行了展望. 相似文献
822.
Levels of polychlorinated dibenzo-p-dioxins and dibenzofurans in China and chemometric analysis of potential emission sources 总被引:2,自引:0,他引:2
Aimed to give a preliminary image of dioxin pollution in China, chemometric analysis was performed to determine background dioxin levels during the period 1994-2002 and potential emission sources. Using principal components analysis (PCA), the congener profiles of 71 sediment samples from rivers, lakes, and sea bays around China were compared with the congener profiles of various known or suspected industrial, residential, and municipal dioxin sources to determine whether the dioxin residues typically found in a broad range of potential environmental sources could explain the presence of these chemicals in China. It was found that the background dioxin levels of China were similar to those of lightly polluted samples from other countries during the period 1994-2002. Primary ferrous ore sintering and secondary lead and aluminum smelters were the major sources of dioxin emission in China. Chloranil and wastewater from chemical plants, sodium pentachlorophenate, and pulp bleaching were also important sources of dioxin emission. Open burning of e-waste as well as diesel-fueled and leaded gas-fueled vehicles were additional possible sources of dioxin. In contrast to other countries, in China, flue gases from incineration of municipal waste, hazardous waste, and medical waste might be minor sources of dioxin emission. 相似文献
823.
Health risks of heavy metals in contaminated soils and food crops irrigated with wastewater in Beijing, China 总被引:24,自引:0,他引:24
Khan S Cao Q Zheng YM Huang YZ Zhu YG 《Environmental pollution (Barking, Essex : 1987)》2008,152(3):686-692
Consumption of food crops contaminated with heavy metals is a major food chain route for human exposure. We studied the health risks of heavy metals in contaminated food crops irrigated with wastewater. Results indicate that there is a substantial buildup of heavy metals in wastewater-irrigated soils, collected from Beijing, China. Heavy metal concentrations in plants grown in wastewater-irrigated soils were significantly higher (P相似文献
824.
825.
黄地老虎NPV增效作用的研究 总被引:3,自引:0,他引:3
对黄地老虎核型多角体病毒(AsNPV)的多角体蛋白和病毒粒子组分进行分离纯化,分别与棉铃虫核型多角体病毒(HaNPV)混合感染三龄棉铃虫幼虫,发现此二种组分均能够提高棉铃虫幼虫的死亡率,缩短半致死时间,降低半致死浓度。使用酶联免疫吸附测定(ELISA)技术检验AsNPV多角体蛋白和病毒粒子与粘虫颗粒体病毒增效因子(PsGV-SF)和HaNPV多角体蛋白的同源性,发现AsNPV多角体蛋白和病毒粒子与 相似文献
826.
从顽拗植物荔枝中提取基因组DNA技术的研究 总被引:35,自引:0,他引:35
针对荔枝体内含有大量影响DNA提取质量的多酚等次生代谢物质的特点,在常规SDS和CTAB提取方法的基础上做了技术改进,即在核裂解之间先破碎细胞,将存在于细胞质中次生物质除去后再裂解细胞核,同时加入活性炭以吸咐杂质反复清洗几次后加入核型解液释放基因组DNA,纯化之后经外观和电泳检测,以及D260nm和D280nm比值的测定,限制性内切酶反应,PCR扩增的结果表明,用改进方法提取的DNA无论在纯度上还 相似文献
827.
828.
During early development in fish, phospholipase A2 (EC 3.1.1.4) regulates membrane lipid modifications, which relates to changes in environmental conditions and provision of
fatty acids required for metabolic energy substrates and prostaglandin biosynthesis. A method to analyze phospholipase A2 in rat tissues has been modified to measure its activity in embryonic Atlantic halibut (Hippoglossus hippoglossus L.). Egg and embryo samples were collected during the 1994 spawning season. Enzyme activity was undetectable at fertilization
but in 10-d embryos was 230 pmol mg−1 h−1 (at 20 °C) and increased by ∼120% at hatch (17-d). Significant alterations in the fatty acid composition of important phospholipids,
phosphatidylcholine (PC) and phosphatidylethanolamine (PE), were also observed. The content of some critical polyunsaturated
fatty acids, and the ratio of unsaturated/saturated fatty acids, declined significantly over development. Acyl-chain restructuring
mediated through the activity of phospholipase A2, coupled with other observed lipid changes (significant increases in the PC/PE ratio and cholesterol content), would produce
a decreased fluidity of membranes during embryonic development, coinciding with the predicted upward movement of larvae in
the water column. Arachidonic acid (20:4n-6) removed from PE could serve as a precursor for biosynthesis of 2-series prostaglandins, and eicosapentaenoic acid (20:5n-3) from PC is a likely source for other prostaglandin types. Despite removal of polyunsaturated fatty acids, there was an
overall increase in lipid and fatty acid concentration, which can be attributed to amino acid catabolism during early developmental
stages.
Received: 9 September 1996 / Accepted: 8 September 1997 相似文献
829.
贵州省煤中挥发性和半挥发性微量元素分布规律的初步研究 总被引:25,自引:0,他引:25
研究了贵州省四大煤田不同层位、不同煤种中Hg,As,Se,Pb,Cd,Tl,Zn,Sb等挥发性和半挥发性微量元素的分布规律。贵州省原煤中Hg,As,Se,Pb,Cd,Sb等元素的含量高于地壳克拉克值;Tl,Zn等的含量低于地壳克拉克值;另外,除了Pb,Zn外,贵州省原煤中Hg,As,Se,Cd的含量远远高于东北和内蒙古东部地区。微量元素聚类分析结果表明,Hg,As,Sb可能主要分布于煤的黄铁矿中;Zn和Se可能主要赋存于闪锌矿中;Cd可能部分存在于硫化物中,部分存在于硅酸盐矿物相中;Pb可能主要存在于硅酸盐矿物相中;而Tl在煤中的赋存形式较复杂。 相似文献
830.
1—硝基芘对斜生栅藻的毒性研究 总被引:2,自引:2,他引:0
酿要用评价化学品对藻类毒性的标准实验方法,研究了1-硝基芘对斜生栅藻生长的影响,得到了1-NP抑制斜生栅藻生长的EC50(96h)为0.1824mg.1^-1,实验结果还表明,1-NP对藻生长的抑制作用随时间的延长而增加,抑制物性表现为特久且强度大,抑制机理为不可逆性抑制。 相似文献