Profiling kidney microRNAs from juvenile grass carp (Ctenopharyngodon idella) after 56 days of oral exposure to decabromodiphenyl ethane

Grass carp(Ctenopharyngodon idella) is one of the most important species in China.Decabromodiphenyl ethane(DBDPE) is a brominated flame retardant that has been used widely in industry, and has been observed to accumulate in the tissues of fish from South China. Evidence has shown that DBDPE is toxic to aquatic animals, but the molecular response has been unclear. MicroRNAs(miRNAs) are small noncoding and negative regulatory RNAs that are 20–24 nucleotides in length, which are involved in a wide range of biological processes. We took advantage of deep-sequencing techniques to accurately and comprehensively profile the kidney mi RNA expression of grass carp after 8 weeks of oral exposure to DBDPE. After mapping sequencing data to the genome and Expressed Sequence Tags(ESTs) of grass carp, we identified 493 miRNAs in the sequenced grass carp samples, which included 51 new miRNAs. The results indicated that 5 miRNAs were significantly down-regulated and 36 miRNAs were significantly up-regulated(FDR 0.001,1.5-fold change) after DBDPE exposure. Real-time quantitative PCR(RT-q PCR) was performed on 4 miRNAs from the two samples, and the sequencing and RT-q PCR data were consistent. This study provides the first comprehensive identification of grass carp miRNAs, and the first expression analysis of grass carp miRNAs following DBDPE exposure.The results indicated that miRNAs have potential for use as biomarkers.     

区域大气环境容量及其区域配置研究

以重庆市涪陵区为例,在了解区域大气环境功能区划及区域大气环境质量现状的前提下,采用A-P值法,对涪陵区十三五期间大气环境容量进行研究,并对辖区内的污染负荷进行预测,在此基础上,对大气污染总量控制指标进行分配.研究结果表明,在涪陵区化工园区的二氧化硫排放量已达到或超过总量控制指标,需要制定削减计划,新兴工业园区污染物排放量虽远小于总量控制指标,但根据城市发展规划需进行结构优化与合理布局.     

论长江经济带大气污染防治的若干问题与防治对策

长江经济带是世界上最大的内河产业带和制造业基地,建设长江经济带是新时期中国三大国家发展战略之一,积极、有效地保护长江经济带生态环境具有非常重要的战略意义。本研究从识别突出的大气污染防治问题入手,深入分析大气污染的主要驱动力产业与能源结构,以问题为靶向,提出长江经济带的大气污染防治策略。研究结果发现,占国土面积21%的长江经济带排放了全国34%的二氧化硫、32%的氮氧化物、28%的烟粉尘、44%的挥发性有机物、43%的氨,单位面积污染物排放强度是全国平均水平的1.3~2.1倍,污染物排放远超环境容量。长江经济带126个城市中,6项主要大气污染物年平均浓度全部达标的城市比例不到1/3。颗粒物是影响城市达标的主要污染物。长江经济带产业结构偏重和能源消费以煤为主,是造成大气污染排放量的主要源头,是大气污染的主要驱动力。以解决突出的大气环境问题为核心,将环境质量作为大气污染防治的底线,持续推进空气质量改善,重点措施上,从大气污染驱动力着手,提出优化产业与能源结构、深化多污染物协同控制、推进区域联防联控等大气污染防治对策建议。     

预防富氧积聚的安全防护措施研究

氧气在生产、使用、贮存和运输过程中经常发生泄漏爆炸事故,给人民的生命财产造成巨大的损失。为了避免和减少氧气泄漏爆炸事故的发生,阻断氧气生产、贮运装置形成富氧积聚是关键。本文针对富氧积聚的形成及其危害特性,从技术方面进行系统研究和探索,提出了科学预防富氧积聚的安全对策与措施。     

老君山综合生态系统管理示范区社区居民环境意识现状调查研究

建立了老君山综合生态系统管理项目示范区社区居民环境意识现状调查问卷的评估体系,分析了调查结果,针对调查对象在环境知识、环境意识、环保技能、环保态度、环境生态价值观等方面反映出的问题提出了建议.     

Application of digital technologies about water network in steel industry

To improve the efficiency of water use and reuse, a digitalized water network system was implemented in a steel industrial complex. The system consisted of four parts: online instrument and sensing devices, programmable logic controller, data communication network and control center based on expert knowledge. The system achieved real-time monitoring, diagnosis, and early warning of cooling water system using data collected inline, and enabled data connectivity and transmission of self-developed software applications on personal computer. This research used a steel enterprise as a case study. After the analysis of its cooling water system and water-saving potential using water balance test, a digitalized water network was evaluated and implemented, and we believe this network can be suitable for steel industry in general.     

粪便与厨余垃圾现场处理研究

研究了粪便与厨余垃圾的源头真空管道收集及现场生物处理技术,根据系统工艺设计方案进行了分单元的小试.研究表明:真空便器的冲水量约为1L/次,破碎1kg垃圾用水0.4～0.6L;厌氧消化反应器进料粪便占40%,厨余垃圾占60%,水分控制在93%左右,C/N=25∶1左右,pH控制在6.2～7.3之间,最佳搅拌频率为6h/d,总停留时间28d;ABR反应器对混合上清液的COD去除率可达91%;用驯化得到的解磷、解钾菌株与消化污泥混合制取菌肥,解磷菌对土壤有效磷增强度67.5%,解钾菌对土壤有效钾增强度33.4%.