The gene for the catalytic domain of thermostable endo-β-1,3-glucanase (laminarinase) LamA was cloned from Thermotoga maritima MSB8 and heterologously expressed in a bioengineered Synechococcus sp. PCC 7002. The mutant strain was cultured in a photobioreactor to assess biomass yield, recombinant laminarinase activity, and CO2 uptake. The maximum enzyme activity was observed at a pH of 8.0 and a temperature of 70°C. At a CO2 concentration of 5%, we obtained a maximum specific growth rate of 0.083 h–1, a biomass productivity of 0.42 g?L–1?d–1, a biomass concentration of 3.697 g?L–1, and a specific enzyme activity of the mutant strain of 4.325 U?mg–1 dry mass. All parameters decreased as CO2 concentration increased from 5% to 10% and further to 15% CO2, except enzyme activity, which increased from 5% to 10% CO2. However, the mutant culture still grew at 15% CO2 concentration, as reflected by the biomass productivity (0.26 g?L–1?d–1), biomass concentration (2.416 g?L–1), and specific enzyme activity (3.247 U?mg–1 dry mass).
Environmental Science and Pollution Research - Risk assessing newly synthesized chemicals prior to their applications is extremely important, if we want to ensure substitution of risky chemicals... 相似文献
Aerosol samples of PM10 and PM2.5 are collected in summertime at four monitoring sites in Guangzhou, China. The concentrations of organic and elemental carbons (OC/EC), inorganic ions, and elements in PM10 and PM2.5 are also quantified. Our study aims to: (1) characterize the particulate concentrations and associated chemical species in urban atmosphere (2) identify the potential sources and estimate their apportionment. The results show that average concentration of PM2.5 (97.54 μg m−3) in Guangzhou significantly exceeds the National Ambient Air Quality Standard (NAAQS) 24-h average of 65 μg m−3. OC, EC, Sulfate, ammonium, K, V, Ni, Cu, Zn, Pb, As, Cd and Se are mainly in PM2.5 fraction of particles, while chloride, nitrate, Na, Mg, Al, Fe, Ca, Ti and Mn are mainly in PM2.5-10 fraction. The major components such as sulfate, OC and EC account for about 70–90% of the particulate mass. Enrichment factors (EF) for elements are calculated to indicate that elements of anthropogenic origins (Zn, Pb, As, Se, V, Ni, Cu and Cd) are highly enriched with respect to crustal composition (Al, Fe, Ca, Ti and Mn). Ambient and source data are used in the multi-variable linearly regression analysis for source identification and apportionment, indicating that major sources and their apportionments of ambient particulate aerosols in Guangzhou are vehicle exhaust by 38.4% and coal combustion by 26.0%, respetively. 相似文献
This study aims to apply Moderate Resolution Imaging Spectroradiometer (MODIS Data) to monitor water quality parameters including chlorophyll-a, secchi disk depth, total phosphorus and total nitrogen at Chaohu Lake. In this paper, multivariate regression analysis, Back Propagation neural networks (BPs), Radial Basis Function neural networks (RBFs) and Genetic Algorithms-Back Propagation (GA-BP) were applied to investigate the relationships between water quality parameters and the MODIS bands combinations. The study results indicated that a simple, efficient and acceptable model could be established through multivariate regression analysis, but the model precision was relatively low. In comparison, BPs, RBFs and GA-BP were significantly advantageous in terms of sufficient utilization of spectra information and model reliance. The relative errors of BPs, RBFs and GA-BP were below 35%. Based on method comparison, it can be concluded that GA-BP is more suitable for simulation and prediction of water quality parameters by applying genetic algorithm to optimize the weight value of BP network. This study demonstrates that MODIS data can be applied for monitoring some of the water quality parameters of large inland lakes. 相似文献
Total suspended particle samples and gas phase samples were collected at three representative sampling sites in the southeastern suburb of Beijing from March 2005 to January 2006. The samples were analyzed for 16 US EPA priority PAHs using GC/MS. Concentrations of Sigma PAHs in particle and gas phases were 0.21-1.18 x 10(3) ng m(-3) and 9.5 x 10(2) ng-1.03 x 10(5) ng m(-3), respectively. PAH concentrations displayed seasonal variation in the order of winter>spring>autumn>summer for particle phase, and winter>autumn>summer>spring for gas phase. Partial correlation analysis indicates that PAH concentrations in particle phase are negatively correlated with temperature and positively correlated with air pollution index of SO(2). No significant correlation is observed between gas phase PAHs and the auxiliary parameters. Sources of PAH are identified through principal component analysis, and source contributions are estimated through multiple linear regression. Major sources of atmospheric PAHs in the study area include coal combustion, coke industry, vehicular emission and natural gas combustion. 相似文献
