Growth and production of the inshore marine copepod Pseudodiaptomus marinus in the central part of the Inland Sea of Japan

The growth and production of the inshore marine copepod Pseudodiaptomus marinus was studied in the central part of the Inland Sea of Japan. The stage-specific growth rate was determined under controlled laboratory conditions by examining the length-weight relationship and development rates at various temperatures. The stage duration was short and constant from NII to CII, beyond which development was retarded. Males developed faster than females in CIV and CV. The specific growth rate was highest in copepodite stages followed by the nauplii and adult females (=egg production rate). The daily production of P. marinus was estimated from the stage-specific growth rate and stage-specific abundance in nature as the sum of the individual stages. The production changed seasonally with water temperature and population biomass. Daily production and biomass (P/B) ratios increased linearly with temperature. Total annual production was 20.7 mg C m^-3 yr^-1.     

Evaluation of fodder quality of straw of different landraces of rice (Oryza sativa L.) under cultivation in the Kumaun Region of Indian Central Himalaya

Thirty landraces of rice collected from various locations, across an elevational gradient (1150–2400 m asl), in Kumaun, Central Himalaya and VL-206 (a high-yielding variety recommended for rainfed mountain area) have been evaluated, both in terms of quantity and nutritional quality of straw. Data were collected to estimate the percentage share of fodder in the overall biomass produced. The total biomass harvested at maturity was taken as productivity. In view of the severe scarcity of fodder in hill regions, particularly during winter months, straw was also examined for quality-linked biochemical parameters and dry matter digestibility (DMD) of fodder. The Crude Protein (CP) values ranged from 2.21% (Nandhani) to 6.33% (Dalbadal), and in saceo DMD (nylonbag technique) was highest in Kaladur (48.84%) and lowest in Kantolia (32.25%). While the CP in VL206 was within the range recorded for various landraces (3.61%), DMD value (27.29%) was lower than that of all the landraces tested. In saceo DMD data indicate that the fodder quality of landraces is better than that of the introduced variety. The values for neutral detergent fibre (NDF) ranged from 63.64% (Sabhawati) to 78.46% (Chhotiya) among the rainfed landraces, while in the irrigated landraces, Thapchini had more NDF (87.50 %), as compared to Kantolia (73.88%) and VL206 (74.91%). Values for the acid detergent fibre (ADF) varied from 42.70% (Danbasmati) to 54.58% (Nandhani) in the rainfed group; ADF was slightly higher in Thapchini (49.41%) in comparison to Kantolia (47.19) and VL206 had the highest (56.80%) ADF content. The observed differences with respect to straw productivity, chemical composition and in saceo DMD in various landraces, even when grown under similar conditions at one place, clearly indicate the richness of the gene pool of rice being maintained, through cultivation, in the Kumaun hills. It may be mentioned that agriculture in the hills is biomass based, and livestock form an integral component of subsistence farming.     

Effects of water velocity on bulk water quality and biofilm population structure in drinking water distribution systems北大核心CSCD

To study the effect of flow velocity on drinking water distribution systems, bulk water quality was monitored over 28 days, biomass was measured, and 16S rDNA was sequenced on the 28th day using a water distribution simulation system. The relationship between bulk water quality and biofilm was statistically analyzed. Flow velocity of 0.5 m/s yielded the most total organic carbon (TOC) (5.26 ± 0.17 mg/L) in the bulk water, the most bulk water bacteria (lg (n+1/mL-1) = 4.79 ± 0.02), the worst bulk water quality, and the most biofilm bacteria (lg (n+1/cm-2) = 5.48 ± 0.06). A Pearson correlation analysis showed the total number of biofilm bacteria was positively correlated with conductivity (R = 0.73, P < 0.01), turbidity (R = 0.87, P < 0.001), TOC (R = 0.94, P < 0.001), and total bacteria (R = 0.92, P < 0.001), and was negatively correlated with residual chlorine (R = -0.68, P < 0.05). Biofilm diversity was high under the low (0.1 m/s) and high (2.5 m/s) flow rates, but the bacterial diversity of biofilm was the lowest at the 0.5 m/s flow rate, in which Proteobacteria dominated the biofilm community structure. These results suggest that flow velocity affects bulk water quality and biofilm population structure, and water quality and biofilm population structure are interrelated, which provides the theoretical basis for research on biofilms in drinking water distribution systems. © 2018 Science Press. All rights reserved.     

Nitrogen export from an agriculture watershed in the Taihu Lake area, China

Temporal changes in nitrogen concentrations and stream discharge, as well as sediment and nitrogen losses from erosion plots with different land uses, were studied in an agricultural watershed in the Taihu Lake area in eastern China. The highest overland runoff loads and nitrogen losses were measured under the upland at a convergent footslope. Much higher runoff, sediment and nitrogen losses were observed under upland cropping and vegetable fields than that under chestnut orchard and bamboo forest. Sediment associated nitrogen losses accounted for 8-43.5% of total nitrogen export via overland runoff. N lost in dissolved inorganic nitrogen forms (NO(3-)-N + NH4+-N) accounted for less than 50% of total water associated nitrogen export. Agricultural practices and weather-driven fluctuation in discharge were main reasons for the temporal variations in nutrient losses via stream discharge. Significant correlation between the total nitrogen concentration and stream discharge load was observed. Simple regression models could give satisfactory results for prediction of the total nitrogen concentrations in stream water and can be used for better quantifying nitrogen losses from arable land. Nitrogen losses from the studied watershed via stream discharge during rice season in the year 2002 were estimated to be 10.5 kg N/ha using these simple models.     

Influence of operating conditions on nitrous oxide formation during nitritation and nitrification

Nitrous oxide (N₂O), a strong greenhouse gas, can be produced by ammonium-oxidizing bacteria (AOB) as a by-product of ammonium oxidation and can potentially be formed in all types of nitrification processes. However, partial nitritation has been reported to cause significantly higher N₂O emissions than complete nitrification. In the study presented here, the mechanisms and factors that drive N₂O formation by AOB were investigated with respect to different operational strategies to achieve nitrite accumulation base on combined evaluation of oxygen uptake rate (OUR) and N₂O formation rate. On the one hand, N₂O formation during partial nitritation and nitrification in a continuously stirred tank reactor (CSTR) with continuous aerobic conditions was observed. On the other hand, the effect of intermittent aeration on N₂O formation during nitrification was investigated. The presence of nitrite, the extend of sludge-specific ammonium loading, low oxygen concentration, and transition from aerobic to anoxic conditions significantly increased N₂O formation in this reactor independently from each other, indicating that different formation pathways, supposedly via nitrite or hydroxylamine, were active.     

Identification of the dimethylbenzyl mercapturic acid in urine of rats administered with 1,2,4-trimethylbenzene

A study was undertaken of the mercapturic acid metabolism of 1,2,4-trimethylbenzene in the rat. Of three regioisomeric dimethylbenzyl mercapturic acids, i.e. 2,4-, 2,5- and 3,4-dimethylbenzyl isomers, the third isomer was not found in the urinary mercapturic acid isolated by preparative HPLC, from the comparison of NMR spectrum of the isolate with those of authentic specimens. The urinary mercapturate was then assigned to 2,4- and/or 2,5-dimethylbenzyl isomers. The excretion rate of the mercapturic acid was 14-20% of dose as 2,4-dimethylbenzyl isomer.     

Fractionation of fulvic acids: characteristics and complexation with copper

Two fulvic acid samples isolated from Suwannee river (International Humic Substance Society) and Feeitsuey reservoir were subjected to gel filtration chromatography (GFC) for molecular size fractionation. The GFC-eluted samples were separated into three groups corresponding to the molecular weight ranges: < 220, 220-1000, and 1000-4000. Fluorescence quenching techniques were employed for determining the conditional stability coefficient and kinetic parameters of copper complexation with the three fractions of fulvic acids. Experimental conditions were pH 6, 5 x 10(-5)m total copper and 5 mg C litre(-1) of fulvic acids. The conditional stability coefficients of the fulvic acid fractions were in the order of 0.9-3.3 x 10(5)m(-1), and the forward and reverse rate constants were in the order of 6.9-12.4 x 10(3)m(-1) s(-1) and 3.5-8.0 x 10(-2) s(-1). Information could be useful in modelling copper transport in the hydrosphere.     

Green manure plants for remediation of soils polluted by metals and metalloids: Ecotoxicity and human bioavailability assessment

Borage, white mustard and phacelia, green manure plants currently used in agriculture to improve soil properties were cultivated for 10 wk on various polluted soils with metal(loid) concentrations representative of urban brownfields or polluted kitchen gardens. Metal(loid) bioavailability and ecotoxicity were measured in relation to soil characteristics before and after treatment. All the plants efficiently grow on the various polluted soils. But borage and mustard only are able to modify the soil characteristics and metal(loid) impact: soil respiration increased while ecotoxicity, bioaccessible lead and total metal(loid) quantities in soils can be decreased respectively by phytostabilization and phytoextraction mechanisms. These two plants could therefore be used for urban polluted soil refunctionalization. However, plant efficiency to improve soil quality strongly depends on soil characteristics.     

Cadmium stress alters gene expression of DNA mismatch repair related genes in Arabidopsis seedlings

Cadmium (Cd) is a non essential element, and is a widespread environmental pollutant. Exposure to Cd can result in a variety of adverse health effects in plant and humans. In the current study, Arabidopsis seedlings were used as a bio-indicator of Cd pollution. Seedlings were grown on MS media containing 0-6.0 mg L(-1) Cd for 18 days, and the gene expression patterns were used to link increased Cd exposure with progressive biological effects. Reduction of total soluble protein content in shoots of the Arabidopsis seedlings occurred with increase in Cd concentrations. For the gene expression patterns, seven genes known to be involved in cell division and DNA mismatch repair (MMR) system were investigated by semi-quantitative RT-PCR, and normalized using 18S rRNA gene expression. Expression of the proliferating cell nuclear antigen 2 (atPCNA 2), MutS 3 homolog (atMSH 3) and MutL1 homolog (atMLH1) genes in shoots of Arabidopsis was strongly induced by exposure to 0.75 mg L(-1) Cd, but were repressed by other Cd concentrations whereas exposure to 0.75-6 mg L(-1) of Cd resulted in a decreased expression of atPCNA1, atMSH 2, 6 and 7 genes independently of any observable biological effects, including survival, fresh weight and chlorophyll level of shoots. This work demonstrated that specific gene expression changes could serve as useful molecular biomarkers indicative of Cd exposure and related biological effects.