Trophoblast-like cells sorted from peripheral maternal blood using flow cytometry: A multiparametric study involving transmission electron microscopy and fetal dna amplification

Three monoclonal antibodies (MAbs) against trophoblast (GB17, GB21, and GB25) and flow cytometry were used to sort trophoblast-like cells (TLCs) from peripheral blood of pregnant women. Sorted TLCs were processed for electron microscopy and fetal DNA amplification of the Y-specific sequences from mothers carrying male fetuses. At the ultra-structural level, most of the nucleated cells had the morphology of leucocytes, suggesting maternal contaminants, and we did not find the characteristic features of the free inter-villous trophoblast cells. Nevertheless, polymerase chain reaction (PCR) analysis showed an amplification of Y-specific sequences in two out of three samples of sorted TLCs. These results suggest that besides the maternal leucocytes, sufficient trophoblast nucleated fetal cells can be obtained using cell enrichment by sorting. This sensitive method holds promise for non-invasive prenatal diagnosis of fetal sex and if sufficient Y(positive) nuclei are found, for the diagnosis of selected numerical chromosome abnormalities.     

可持续旅游及其区域实现途径探讨—以井冈山风景名胜区为例

可持续旅游是 80年代中期以后提出的关于旅游业发展的新思想。经过十多年的不断拓展深化 ,已经得到世界各国的广泛认同 ,并作为跨世纪旅游业发展的战略指导思想。本文从可持续旅游的由来和对可持续旅游内涵实质的界定角度出发 ,结合可持续旅游实现途径的探讨 ,以井冈山风景名胜区为例 ,对风景区旅游业可持续发展进行了深入的探讨 ,为景区的发展规划提出了战略性构想 ,以期能对井冈山风景名胜区旅游资源的可持续利用和旅游业的可持续发展起到抛砖引玉的作用     

企业需要建立人性化的安全管理

由于我国对企业特别是海外来大陆投资的企业，缺乏严格的安全管理手段，致使不少企业屡次出现安全事故。为了减少企业安全事故的发生，提高企业安全度，要求企业在贯彻“安全第一，预防为主”的基本方针的基础上。建立“以人为本。以制度和伦理道德为约束的人性化的安全管理系统”。本文给出了该系统的模型，并对此模型进行了分析和解释，探讨了相关的几个理论基础，最后给出了人性化安全管理的实质。通过该系统的管理执行，使企业在尊重人权、尊重生命的前提下发展壮大，最终实现安全的全球化。     

Study on Assessing Economic Vulnerability of Small Island Regions

The main purpose of this study is to assess economic vulnerability of small island development regions as part of their sustainability constraints. By combining economic and environmental time series data, we assessed a composite index of economic vulnerability which is constructed from three exogenous variables, namely economic exposure, economic remoteness, and economic impact of environmental and natural disasters. We used the Amami Islands, Kagoshima Prefecture, Japan as the case studies for this paper.The results indicated that using a gross island products based valuation index, Kikaijima is the most vulnerable island in the Amami Islands with a composite economic vulnerability index (CEVI) value of 0.678, while by using a per capita based index, Okinoerabujima is considered the most vulnerable island with a CEVI value of 0.680. From the results we also revealed that smaller islands have relative higher vulnerability than the bigger one, which also confirms some previous country-level vulnerability studies.However, it is matter of fact that some islands that have relatively high vulnerability also have good economic performance as shown by their per capita income. In this regard, it can be argued that the success of these small islands could have been achieved in spite of and not because of their inherent vulnerability conditions as an indicator of sustainability constraint. Regarding these findings, we also examined a comparison between vulnerability results and the preliminary concept of an island's resilience in order to capture another perspective on sustainability assessment in a small island region.     

Study on the Balance of Agricultural Water and Land Resources on Ningxia Plain

The article puts forward the process and means of regional water and land balance research, and then from two scenarios which are the balances under natural regulation and human intervention, calculated and analysed the balance between water and land on Ningxia Plain. For the balance under natural regulation named farmland water balance, using farmland water resource balance equation, the research estimated the monthly farmland＂ water balance of 8 major crops for all of the 12 counties on Ningxia Plain in the period of 1960-2001; for the balance under human intervention, the research estimated land-use water balance equation of the counties in 2000, and calculated the balance between land use and water resources including irrigating water of all the 12 counties on Ningxia Plain. Results showed that ①precipitation can not meet the water demand of the crops for growth and development on Ningxia Plain, and water shortage is the primary character of farmland water balance under natural regulation. ②the diversity of water and land balance of different counties is distinctly influenced by the crop structure, water quantity for irrigation and irrigation level. ③Irrigation water could meet the crop water demand on Ningxia Plain in 2000, but there was not much space to expand irrigating cultivated land.     

社会和谐与可持续发展

本文运用系统科学原理阐释了社会和谐的内涵,指出社会和谐须以人与自然和谐为基础,以人与人之间的平等、友爱和同合作为表征,其构建与实现应遵从人类社会的演化规律,依赖予在把握人类社会需求演绎、协同人与自然关系的基础上推动生产力的高效有序发展和自然资源、生态环境的可持续支持.进而从可持续发展角度,提出了人与自然和谐的"双剩余理论",以揭示其间的供瓣枧理和协同准则;推导出了社会收益分配的相对公平系数,以衡量人与人公平要求下的社会和谐.针对国情和战略目标要求,提出了以加强小城镇建设为轴心的城市、小城镇与农村人流、物流、财流和信息流合理聚散的"三元结构"发展模式,以促进我国和谐社会的建设和可持续发展战略的有序实践.     

村级水资源的管理与利用研究--来自南方四个村的案例分析

农村水资源及其相关设施的改革需要更多了解村和户层次上的现实状况和机制安排。以避免改革扭曲和失败。通过对湖北和广西共四个村的村委会、关键知情人及120个农户的调查。在村和农户两个层次上描述水资源及其相关设施的管理与利用，尤其是在干旱年份村和户所采取的策略。并深入分析了农村村级水资源管理与利用的制度安排与问题。得出的结论是。追求水利管理效率的市场化改革需要考虑干旱的威胁，要有基于风险的制度设计和法律保障。     

Dandy – Walker syndrome and corpus callosum agenesis in 5p deletion

5p deletion syndrome commonly known as cri du chat is well described in affected neonates with catlike cry and hypotonia. Karyotyping will usually show a deletion of the short arm of one chromosome 5 with variable breakpoints. Only a few cases have been reported prenatally, and the fetal form of the syndrome has not been clearly individualised. We report a new case of 5p deletion syndrome diagnosed prenatally in association with Dandy–Walker syndrome and agenesis of the corpus callosum. Other brain anomalies have been reported previously, but this unusual association suggests the use of a specific probe in the investigation of these malformations. Copyright © 2005 John Wiley & Sons, Ltd.