Prenatal diagnosis of β thalassaemia by reverse phase HPLC

Reverse phase HPLC of radioactive globin chains has been compared to classical carboxy methyl cellulose chromatography for the prenatal diagnosis of β thalassaemia. The two methods correlated highly (r = 0.97 p < 0.0005) and provided an identical diagnosis for 40 fetal blood samples of fetuses homozygous or heterozygous for β thalassaemia. The HPLC procedure was much faster and required fewer biochemical steps (no globin preparation). It was at least as accurate and more sensitive than the classical chromatography. A single column can be used for 150 analyses and is always ready to be used. Last but not least it is much less expensive than CMC chromatography.     

TTC－脱氢酶活性检测方法的研究

以好氧消化污泥为材料，对影响脱氢酶活性测定的样品前处理、生化培养、酶反应终止剂、三苯基甲Ｚａ（ＴＦ）苯取剂以及萃取条件等问题进行了系统地研究，进而提出了检测脱氢酶活性的常温萃取测定法。这种方法已在剩余污泥好氧消化处理、生物转盘处理生物制品废水以及陶粒柱反应器低温生物预处理饮用水等研究中应用，效果良好。     

Prenatal sonographic assessment of early,rapidly growing fetal cervical teratoma

Although malignant transformation of fetal cervical teratoma is extremely rare, perinatal morbidity is high and usually related to the size of the tumour, which may compromise fetal swallowing and subsequently lead to upper airway obstruction. We present a case in which mid-trimester serial sonography demonstrated markedly rapid early growth of a lesion of this type between 17 and 19 weeks' gestation indicating the aggressive nature of this tumour, assisting parental decision to terminate the pregnancy. Histopathology confirmed grade 3 immaturity of the lesion.     

近期和长远时段内北极生态系统结构受到的影响

生物在物种水平上对全球气候变暖和紫外线B(UV-B)辐射增强的反应受到其群落内其他物种以及生态系统内养分循环的调节,所有的这些反应将会导致生态系统结构的变化.根据高纬度地区坏境因子的可能变化而做的控制试验表明,由夏季变暖而引起苔原植被的变化要小于增加施肥而引起的变化,试验涉及的某些环境因子对北极生态系统的结构有非常强烈地影响,但是这些影响因地区而异,观测结果表明,处于最寒冷地区的植物群落和无脊椎动物群落对全球气候变暖和紫外线B辐射的增强反应最为强烈.尽管微生物量和养分储存量相对稳定,北极无脊椎动物群落还是很可能会对全球变暖产生迅速的反应.试验结果显示,加强紫外线B辐射会改变革兰氏阴性细菌和真菌的群落组成结构,但不会对植物群落的组成产生影响.由夏季气温升高而提高的植物生产力将会控制食物网的动态变化,以苔原植被和亚极地森林植物为基础的食物网中的营养流动会明显地影响到几种优势动物种群数量的周期性波动,在某些年分这些动物的种群数量会达到峰值.小型啮齿动物和食叶昆虫如秋毛虫种群数量的周期性变化则会影响苔原和森林苔原植物的组成结构和多样性,同时也会影响到一些专性捕食者和寄生虫的变化.在暖冬,雪表形成冰壳可能会减少旅鼠的植物食物来源,然而较深的雪也可以使它们免受雪地表面上捕食者对它们的捕食.在芬诺斯堪的亚地区,已有证据表明小型啮齿类动物群落结构和种群动态的显著变化会导致专门以小型啮齿类动物为食物的捕食者的数量减少.气候还可能改变昆虫在白桦森林生态系统中的作用,因为暖冬可以增加这些昆虫卵的成活率,并且扩大其分布范围.此外,在夏季困扰驯鹿的昆虫会由于夏季气候的变暖而扩大其分布范围、增加种群数量并且种群更为活跃;同时在另一方面也会对驯鹿不利,即那些昔日驯鹿/北美驯鹿的避难场所--冰川和未融的成片的雪--在这样温暖的夏季则可能会消失.     

Effect of long-term fertilization on soil nitrate distribution

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Extraction of DNA from amniotic fluid cells for the early prenatal diagnosis of genetic disease

Ten-ml samples of amniotic fluid were taken from pregnancies being terminated at 8–14 weeks' gestation. DNA was extracted from the amniotic cells by sequential centrifugation and analysed using the polymerase chain reaction (PCR). Fifteen samples were analysed for evidence of maternal contamination using Mfd5 oligo-nucleotide primers for repeat polymorphisms. Ten amniotic fluid samples were tested for the Delta-F₅₀₈ deletion characteristic of cystic fibrosis to demonstrate a diagnostic application for the technique. In each case, DNA extracted from fetal tissue from the same pregnancy was included in the controls. In 14 of the 15 cases tested with the Mfd5 primers, both the amniotic fluid DNA and the fetal DNA showed no evidence of contaminating DNA. In one case, neither the amniotic fluid cells nor the fetal cells yielded results. In nine of the ten cases tested with the Delta-F₅₀₈ primers, the amniotic fluid cell DNA provided accurate information about the genetic status of the fetus; in the tenth, the fetal DNA failed to amplify. The results indicate that adequate DNA can be extracted from amniotic fluid from 8 weeks' gestation onward and these samples are suitable for prenatal diagnosis using PCR.     

Vitamins,folic acid and neural tube defects: Comments on investigations in the United States

No clear answer concerning whether multivitamin/folate supplementation prevents neural tube defects (NTDs) is provided by three studies in the United States. All these studies are occurrence in nature, no recurrence studies having been conducted. The Atlanta Birth Defects Study is subject to pronounced memory and recall biases, the length between event and interview being as long as 16 years. In a second study (Boston University), objections can be raised to certain aspects of the experimental design, and the claim that 22 per cent of women started vitamins sufficiently early after pregnancy diagnosis to influence NTD formation is suspicious. Our NICHD case control study of 541 women in California and Illinois revealed no evidence for multivitamins or folic acid preventing NTDs. U.S. public policy-makers face difficulties in applying results of recurrence or occurrence studies in high-risk areas to low-risk areas in the U.S.     

Prenatal pleural effusion associated with congenital pulmonary lymphangiectasia

A pleural effusion associated with congenital pulmonary lymphangiectasia was detected in a fetus in utero but was absent at the time of delivery. The pleural effusion was unilateral although the disease involved both lungs. In this case there was an association between polyhydramnios and congenital pulmonary lymphangiectasia.     

The influence of source and timing of nitrogen fertilizers on yield and nitrogen use efficiency of four sweet potato cultivars

Nitrogen (N) availability is a very important determinant of crop yield. It is also one of the most expensive inputs in crop production. Thus, the efficient management of N by farmers with limited resource is a very important part of successful soil and crop management system. A field study was conducted on a Norfolk sandy loam soil at the George Washington Carver Agricultural Experiment Station, Tuskegee, AL, to evaluate the influence of fertilizer source and timing on the yield of four sweet potato [Ipomoea batatas (L.)] cultivars. Two N sources (urea, CO(NH₂)₂ and ammonium nitrate, NH₄NO₃), four sweet potato cultivars (‘Georgia Jet’, GA-Jet; ‘TU-82-155’, TU-155; ‘TU-1892’; and ‘Rojo Blanco’, Rojo-Bl) and three times (zero, single, and split applications) were used in a factorial designed experiment. Plants were harvested after 80 and 120 days and total and marketable yields determined. Nitrogen recovery efficiency, yield efficiency and physiological efficiency were also determined. Total and marketable yields of early maturing cultivars were significantly higher than of late maturing cultivars (P<0.05). The single application of N resulted in significantly higher yield in storage roots than split application (P<0.05). Physiological efficiency values were highly correlated with total marketable yields. Recovery rates increased with time. Late maturing cultivars tended to have higher N recovery and physiological efficiency than early maturing cultivars. These results suggest that cultivar maturity group should play an important part in N fertilization recommendations for sweet potato.