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201.
202.
For the screening of the risk from environmental contamination, the cytotoxic/genotoxic effects of various model pollutants were determined using an in vivo system comprised of human HeLa cells; the ecotoxicity was also determined using the acute and genotoxicity tests on two aquatic sentinel species widely used in biomonitoring, namely, freshwater crustacean, Daphnia magna and larva of aquatic midge, Chironomus tentans. Nonylphenol (NP), bisphenol A (BPA), bis(2-ethylhexyl) phthalate (DEPH) and paraquat dichloride (PQ) were used as the model pollutants. The results showed that exposure of HeLa cells to NP, BPA and DEHP was sufficient for the expression of noticeable genotoxic and cytotoxic effects. Ecotoxicity results showed that, as expected, D. magna was more sensitive than C. tentans to chemical exposure. BPA may exert a genotoxic effect on D. magna and C. tentans, given that DNA strand breaks increased in both species exposed to this compound, whereas NP-induced DNA damage occurred only in C. tentans. In vivo genotoxic data obtained in aquatic sentinel species could provide valuable information for freshwater quality monitoring. From the results of the present study, the use of cytotoxic, genotoxic and ecotoxic tests using human cell system, as well as, biomonitoring species, seems to be relevant for preliminary evaluation of the human health and ecological effects of pollutants and thus, a promising screening tool for environmental monitoring and risk assessment. 相似文献
203.
Sulphamethazine in poultry manure changes carbon and nitrogen mineralisation in soils 总被引:1,自引:0,他引:1
Yasser M. Awad Avanthi Deshani Igalavithana Young Han Lee Yeon-Kyu Sonn Adel R. A. Usman 《Chemistry and Ecology》2016,32(10):899-918
Antibiotics are newly emerging organic pollutants in manure, soil, vegetables and water. Animal manure application might be leading to the accumulation of antibiotics in the farmland. However, the effect of sulphamethazine (SMZ) on the soil microbial community was scarcely investigated. This study was aimed to evaluate the impact of SMZ on poultry manure, on the structure and function of microbial community, carbon mineralisation, and changes in nitrogen forms in soil via an incubation experiment lasting 56?d. The treatments consisted of poultry manure at 1% wt (PM), PM containing 20?mg?kg?1 SMZ (PM?+?20SMZ) and PM containing 100?mg?kg?1 SMZ (PM?+?100SMZ), along with the untreated soil (control). Solid phase extraction was performed to measure the SMZ concentration in soils using high-pressure liquid chromatography. The cumulative CO2-C was increased in all treated soils over the incubation period compared to the control. The PM?+?100SMZ had the highest increase in cumulative CO2-C from the soil at 56?d of incubation. The treatment of PM?+?20SMZ showed a short-term decrease in nitrification rate in the soils at 1?d by altering the microbial community composition with 17% dissimilarity and decreasing the abundance of bacteria compared to PM-treated soil. The PM?+?100SMZ increased C mineralisation in the soil. 相似文献
204.
Christopher Young Sabine Hähndel Bonaventura Majolo Oliver Schülke Julia Ostner 《Behavioral ecology and sociobiology》2013,67(10):1665-1677
Dominant mammalian males should gain a reproductive advantage due to their greater fighting abilities. However, the extent to which they can monopolise access to females varies across species. In primates and recently other mammalian species, the Priority of Access (PoA) model is commonly used to measure the degree to which male rank and female receptive synchrony affect mating skew. Few studies have examined the factors which lead to deviations from the expectations of the model. Here, we investigate male mating skew in wild Barbary macaques (Macaca sylvanus). We examined four of the main factors which affect male mating success: the roles of male rank, female receptive synchrony, coalitionary activity and female behaviour. We found that male mating was skewed up the hierarchy, but there was a large deviation from the PoA model's expectations with high-ranked males not gaining as big a share as expected. Females frequently initiated sexual encounters, predominantly with mid-ranked males, increasing their mating success. Male coalitionary activity independently increased mating success. Frequent associations with females were costly to males as they were the targets of bridging coalitions, decreasing future mating opportunities for the targets. High-ranking males did not increase their mating success directly through bridging coalitions but acted to dilute the effects of female behaviour. By examining different factors affecting mating skew, we are able to show that alternative male and female mating strategies are effective in reducing the monopolisation potential of the dominant male. 相似文献
205.
Shih-Houng Young Jean M. Cox-Ganser Elizabeth S. Shogren Michael G. Wolfarth Shengqiao Li James M. Antonini 《毒物与环境化学》2013,95(4):806-823
It is observed that 1?→?3-β-glucan, a major cell wall component of fungi, induces pulmonary inflammation. There is inconsistency in determining the correlation between the levels of glucan measured by current extraction methods and the respiratory inflammation observed in individuals or lab animals exposed to environmental dust samples. The glucan-specific limulus amebocyte lysate (G-LAL) method was used after extraction with dimethyl sulfoxide (DMSO) or sodium hydroxide (NaOH) to analyze the glucan content of office dust samples collected from a water-damaged building. C3HeB/FeJ mice, an endotoxin-sensitive strain, were treated with different dust samples (2.5?mg?kg?1 body weight) or saline (vehicle control) by pharyngeal aspiration. At 1?day after aspiration, bronchoalveolar lavage (BAL) was performed, and lung inflammation and injury were assessed by measuring: (1) neutrophil (PMN) infiltration, (2) inflammatory cytokine (IL-6, IL-10, MCP-1, IFN-γ, TNF-α, and IL12-p70) levels, and (3) albumin and lactate dehydrogenase in recovered BAL fluid. Both DMSO and NaOH extraction increased the detection of glucan by approximately 20-fold compared to water extraction. However, only the DMSO extraction method showed a statistically significant positive correlation between 1?→?3-β-glucan and albumin levels, total numbers of BAL, polymorphonuclear leukocytes (PMNs) cells recovered, levels of TNF-α, MCP-1, and IL-6. In conclusion, 1?→?3-β-glucan is a potent inflammatory agent in dust samples and DMSO extraction for glucan analysis may prove useful in understanding the impact of environmental contamination by glucans on lung disease. 相似文献
206.
Seung?Hyeon?Kim Young?Kyun?Kim Sang?Rul?Park Wen-Tao?Li Kun-Seop?LeeEmail author 《Marine Biology》2012,159(2):255-267
Seagrass species in the genus Halophila are usually distributed in tropical or subtropical areas, but a Halophila species identified as H. nipponica was first observed in temperate coastal regions of Korea in 2007. Since this species mainly occurs in warm temperate regions
influenced by warm currents, we hypothesized that H. nipponica may exhibit different growth patterns from those of other temperate seagrass species in Korea, instead showing similar growth
dynamics to tropical/subtropical species. The growth and morphology of H. nipponica in relation to coincident measurements of environmental factors were investigated from July 2008 to September 2009 to examine
the growth dynamics of this species. Water temperature at the study site ranged from 9.7°C in January to 25.1°C in August.
Shoot density, biomass, and productivity exhibited significant seasonal variation, increasing during summer and decreasing
during winter. Productivity was severely restricted to nearly ceasing at water temperatures less than 15°C, and winter minimum
growth lasted until May. The optimal temperature for H. nipponica growth was approximately 25°C, which was the maximum water temperature at the study site, and no growth reduction in high
summer water temperature was observed. Thus, H. nipponica on the temperate coast of Korea exhibited a distinctly different growth pattern from those of temperate seagrass species
in Korea, which have shown great reductions in growth at water temperatures higher than 20°C. Higher below- to above-ground
ratio and leaf burial into sediments with shorter leaf petioles during winter might be overwintering strategies in this species.
The growth patterns of H. nipponica at the study site imply that this species still possess the tropical characteristics of the genus Halophila. 相似文献
207.
Accurately surveying shark populations is critical to monitoring precipitous ongoing declines in shark abundance and interpreting the effects that these reductions are having on ecosystems. To evaluate the effectiveness of existing survey tools, we used field trials and computer simulations to critically examine the operation of four common methods for counting coastal sharks: stationary point counts, belt transects, video surveys, and mark and recapture abundance estimators. Empirical and theoretical results suggest that (1) survey method selection has a strong impact on the estimates of shark density that are produced, (2) standardizations by survey duration are needed to properly interpret and compare survey outputs, (3) increasing survey size does not necessarily increase survey precision, and (4) methods that yield the highest density estimates are not always the most accurate. These findings challenge some of the assumptions traditionally associated with surveying mobile marine animals. Of the methods we trialed, 8 x 50 m belt transects and a 20 m radius point count produced the most accurate estimates of shark density. These findings can help to improve the ways we monitor, manage, and understand the ecology of globally imperiled coastal shark populations. 相似文献
208.
Historical trends of trace metals in a sediment core from a contaminated tidal salt marsh in San Francisco Bay 总被引:1,自引:0,他引:1
Sedimentation of metals preserves historical records of contaminant input from local and regional sources, and measurement
of metals in sediment cores can provide information for reconstruction of historical changes in regional water and sediment
quality. Sediment core was collected from Stege Marsh located in central San Francisco Bay (California, USA) to investigate
the historical input of trace metals. Aluminum-normalized enrichment factors indicate that inputs from anthropogenic sources
were predominant over natural input for Ag, Cu, Pb, and Zn. Among these, lead was the most anthropogenically impacted metal
with enrichment factors ranging from 32 to 108. Depth profiles and coefficients of variation show that As, Cd, and Se were
also influenced by anthropogenic input. The levels of these anthropogenically impacted metals decline gradually towards the
surface due to regulation of the use of leaded gasoline, municipal and industrial wastewater discharge control, and closure
of point sources on the upland of Stege Marsh. Although trace metal contamination is expected to be continuously declining,
the rates of decline have slowed down. For lead, it is estimated to take 44, 82, and 153 years to decrease to probable effects
level (112 μg/g), the San Francisco Bay ambient surface sediment level (43.2 μg/g), and the local baseline levels (5 μg/g),
respectively. Some metals in surface sediments (0–6 cm) are still higher than sediment quality guidelines such as the probable
effects level. To further facilitate the recovery of sediment quality, more efficient management plans need to be developed
and implemented to control trace metals from non-point sources such as stormwater runoff. 相似文献
209.
This study investigated efficiency of in situ enhanced biological denitrification of nitrate-contaminated groundwater which employs a well-to-well circulation in a shallow zone where oxygen might give an adverse affect on the denitrification processes. The numerical model developed for the efficiency test included sequential aerobic and nitrate-based respiration, multi-Monod kinetics of reactive components, growth and decay of biomass, and denitrification inhibition associated with the presence of oxygen. Moreover, reaction kinetics for production of toxic intermediates such as nitrite and nitrous oxide were also included in the model. The developed model was applied to the analysis of enhanced in situ denitrification using an injection/extraction well pair. To evaluate the relative remediation effectiveness, comparisons were made between a continuous fumarate injection test (CFIT) system and a pulsed fumarate injection test (PFIT) system, where both systems had the same total fumarate mass injected into the aquifer. The PFIT system was preferable to the CFIT system because of the high possibility of occurrence of clogging in the latter case at the injection well, with no other significant advantages found in either the CFIT or the PFIT system. Accordingly, this developed numerical model is useful to predict and evaluate an in situ bioremediation by denitrification in aquifers. 相似文献
210.
Seagrass beds are highly productive ecosystems and a decline in this habitat has become a global concern in recent decades. This study mapped seagrass at three sites in Port Phillip Bay between 1939 and 2011 and reviewed possible influences on seagrass cover changes. Historical aerial photographs from multiple sources were digitally scanned and orthorectified. Automated image processing techniques incorporating an unsupervised classification combined with minor editing in a GIS were applied to map seagrass cover and analyse variations in the size and distribution of seagrass beds. Large declines in seagrass cover were observed at all three sites after 1998. In contrast to other world-wide observations, these recent declines were preceded by a period of sustained seagrass expansion between the 1960s and 1990s and lower levels of seagrass cover were observed in the 1930s/40s. The recent and earlier low levels of seagrass cover coincided with extended droughts characterised by large reductions in nutrient inputs to the Bay. However, recent declines were not consistent across the Bay with three other sites remaining relatively stable during this period. The sites with large declines are all subject to longshore drift and changes in nearshore sediment transport driven by variations in weather patterns coinciding with extended periods of drought may be important influences on seagrass cover at these locations. 相似文献