Two-Step Cultivation Method for Microbial Disintegration of Tire Rubber Particles

A rubber-degrading strain of Nocardia was cultured on tread rubber particles from a truck tire by a two-step cultivation method. At the first step, the culture medium was either not agitated or stirred at very slow rate of 40 rpm for one or 2 weeks. At the second step, the culture medium was stirred at relatively higher stirring rate of 150 or 300 rpm for seven or six weeks. It was found that the rate of disintegration was greatly increased and the weight losses of the particles with diameters of about 2.3 mm were as high as 40% in the two-step method and only 20–30% in a one-step method in which the stirring rate was kept constant throughout the culture period. With this method, microbial colonization and disintegration were depressed particularly at the corners of the cubic particles and characteristic protuberant structures were observed at the corners after the removal of microbial cells by washing.     

Penetration of soil dust through woven and nonwoven fabrics

Abstract

Several types of fabrics were laboratory‐tested for their effectiveness in worker protection to pesticide‐laden dust encountered in the agricultural environment. Of the applied <100 mesh dust, penetrations through knitted jersey and woven fabrics were greater than 87% and less than 5.8%, respectively. Treatment of woven fabrics with fluorocarbon polymers curtailed penetration by greater than 60%. Nonwoven fabrics allowed less than 0.5% dust penetration. Parathion mixed with 100‐mesh sieved dust resulted in increasing “ppm”; levels with decreasing particle size; extent of parathion conversion to paraoxon was independent of particle size for the sandy loam dust used.     

Biodegradability and Biodegradation of Polyesters

A variety of biodegradable plastics have been developed in order to obtain useful materials that do not cause harm to the environment. Among the biodegradable plastics, aliphatic polyesters such as: poly(3-hydroxybutyrate) (PHB), poly(ε-caprolactone) (PCL), poly(butylene succinate) (PBS), and poly(l-lactide) (PLA) have become the focus of interest because of their inherent biodegradability. However, before their widespread applications, comprehensive studies on the biodegradability and biodegradation mechanisms of these polyesters are necessary. Thus, this paper describes the degradation mechanisms and the effects of various factors on the degradation of polyesters. The distribution of polymer-degrading microorganisms in the environment, different microorganisms and enzymes involved in the degradation of various polyesters are also discussed.     

Utilization of Broken Rice for the Production of Poly(3-hydroxybutyrate)

The feasibility of utilizing non edible rice (broken rice) for production of fine materials such as poly(3-hydroxybutyrate) (PHB) was considered as one of the alternative ways of keeping the environment clean for sustainable development. Thus, production of PHB from broken rice by simultaneous saccharification and fermentation (SSF) was investigated. During the SSF process, the rice (15% w/v) material was hydrolyzed to glucose, which was utilized by Cupriavidus necator for growth and production of PHB. The PHB content reached 38% at 58 h fermentation. The PHB had weight average molar mass (Mw) and polydipersity index of 3.82 × 10⁵ (g/mol) and 4.15, respectively. Differential calorimetric scan of the PHB showed a melting temperature (Tm) of 176 °C. Given that the PHB was a homopolymer (which consisted of (R)-3-hydroxybutyric acid monomers), it was thought that broken rice could be a raw material for production of both PHB and (R)-3-hydroxybutyric acid. This SSF process would not only help in the utilization of broken rice or non edible rice, but would also serve as a model for utilization of other raw materials that contain starch for production of PHB.     

Atmospheric NO2 and CO concentration in Lima,Peru

The atmospheric concentration of NO2 in Lima, Peru was measured through 1 year using passive samplers. The concentration was stable and evident seasonal change was not observed. Also, the distribution of NO2 concentration in entire Lima was monitored twice in different seasons. The average NO2 concentrations at 33 and 27 sites of these monitoring were 17.1 and 15.3 ppb, respectively. NO2 distribution was high in the downtown area and decreased gradually with distance from there. The wind that almost always blew from the ocean had a great influence on it. High NO2 level and the change of CO concentration suggest that the residential area in the northeast side forms a topological channel among hills to blow out the pollutants from the downtown area.     

Determination of dissolved oxygen limitation in aerobic biofilm reactors

The concentration of dissolved oxygen (DO) strongly influences the performance of aerobic biofilm reactors because organic oxidation is limited by the availability of oxygen. However, it is not necessary to maintain a high DO level in the reactors in order to overcome this limitation. Excessive aeration wastes energy. Therefore, the determination of the onset of DO limitation against organic substrate removal in aerobic biofilm reactors is important for their effective operation. This study is aimed at developing an expression to determine the onset of DO limitation and hence to control the aeration system. The expression developed is as follows: , where S_b and C_b are the bulk concentrations of organic substrate and DO, respectively; D_ws and D_wc are the diffusion coefficients of organic substrate and oxygen in the reactors respectively; and R_b is an overall ratio of oxygen consumption to organic substrate removal in the reactors. The latter is the key parameter in the equation, and is determined by the characteristics of the substrate, biofilm, and reactor. In order to measure the value of R_b, the authors have developed a micro-biofilm reactor. The value of R_b was determined to be 0.13 (mg O₂ mg⁻¹ COD_cr) for glucose removal with this reactor. The equation has, subsequently, been verified with data from batch and continuous experiments.     

Effect of Poly(dioxolane) as Compatibilizer in Poly(ɛ-caprolactone)/Tapioca Starch Blends

The influence of poly(dioxolane) (PDXL), a poly(ethylene oxide-alt-methylene oxide), as compatibilizer on poly(ɛ-caprolactone) (PCL)/tapioca starch (TS) blends was studied. In order to facilitate blending; PCL, PDXL and TS must be blended together directly; so that PDXL is partially adhered at the TS surface as shown by scanning electron microscopy. The molecular weight effect of PDXL on the PCL/TS blends showed that mechanical properties of PCL/TS/PDXL blends from low molecular weight (M _n=10,000) and high molecular weight (M _n=200,000) PDXL were rather dependent on TS content. The enzymatic degradability of PCL/TS/PDXL blends using α-amylase increased as the TS content increased but was independent on the dispersion of tapioca starch in the PCL matrix.     

Biparental defence in a spider mite (Acari: Tetranychidae) infesting Sasa bamboo

Summary Field observations on the nesting pattern of Schizotetranychus celarius (Banks) (Acari: Tetranychidae) revealed that many individuals live gregariously in a united nest. Behavioural experiments recorded on video tape recorder and 16 mm film showed that adult males and females can effectively defend their nest and offspring from a phytoseiid predator, larvae of Typhlodromus bambusae Ehara (Acari: Phytoseiidae), through counterattacks. The male spider mite often killed the predator's larva and succeeded in defending offspring and nest completely. Comparison of the behaviour patterns recorded between a nest owner parent S. celarius and two kinds of intruders (predators and conspecific males or females) revealed conspicuous differences. I suggest that there is a kind of biparental care in S. celarius, and that the life-pattern of this species therefore fits the definition of subsociality originally framed for insects.     

Feasibility Study of Airbag Concept Applicable to Motorcycles Without Sufficient Reaction Structure

Objectives: An airbag system for motorcycle applications was developed and marketed in 2006 followed by many research projects on the system. In the airbag system, the bag should be supported during the kinetic energy–absorbing period of a rider in a collision. The previously developed system employed a configuration in which motorcycle structures support the airbag, such as a gauge unit and/or a steering structure. The supporting structure functions to receive the reaction force to hold the airbag during a crash to properly absorb the rider's kinetic energy. However, the previous system requires a larger area for this reaction structure and is applicable only to the motorcycles that can provide that area. To overcome this limitation, we propose an airbag system employing another concept. In this concept, the airbag does not use its vehicle structures as a reaction structure but uses the structures of an opposing vehicle, such as doors and/or pillars of an opposing vehicle. In this project, we aim to verify the effectiveness of the proposed system when installed in a motorcycle that cannot provide a larger area for the reaction structure.

Methods: In the system with this concept, it is assumed that the occupant protection performance is largely affected depending on impact configurations. Accordingly, full-scale motorcycle-to-car crash tests using 125 cm³ scooter-type models with and without the proposed system were conducted in various impact configurations. The 7 impact configurations specified in ISO 13232 were selected as the test configurations. Injury variables and injury indices of head, neck, chest, and abdomen were evaluated with the motorcyclist dummy.

Results: Injury variables and indices obtained from the crash tests with the airbag were compared to those of the baseline tests. In 2 impact configurations, the airbags were supported by the side structures of the opposing vehicle and performed to reduce the injury variable of head and/or chest compared to that of the baseline test.

Conclusion: Through the crash tests, beneficial protection effects of the airbag system were confirmed in particular impact configurations. No significant risk for the occupant due to the airbag was observed in the conducted crash tests. It was concluded that the proposed airbag system has feasibility to reduce rider injury in a collision of a motorcycle without sufficient reaction structure.