Assessing the anti-estrogenic activity of sodium pentachlorophenol in primary cultures of juvenile goldfish (Carassius auratus) hepatocytes using vitellogenin as a biomarker

Both pentachlorophenol and 2,3,7,8-tetrachlorodibenzo-p-dioxin （TCDD） had been studied widely because of their probable anti-estrogenic activity. Sodium pentachlorophenol （PCP-Na）, as a industrial product used in many fields, usually contains a trace of TCDD. The aim of this study was to assess the anti-estrogenic effect of PCP-Na in juvenile goldfish （Gurassius auratus） hepatocyte cultures using vitellogenin （VTG） as the biomarker. The ID50 of PCP-Na was investigated and then a series of concentrations （0.001 0.5 μg/ml） of PCP-Na were evaluated to estimate the anti-estrogenic activity. Results showed that PCP-Na was cytotoxic for hepatocytes even at very low concentration 〈1.21 μg/ml, and it could not induce VTG at any concentrations tested. Since it failed to stimulate VTG production, the possibility of its anti-estrogenic effect was tested, and a well-known anti-estrogenic compound-tamoxifen was used as positive control. PCP-Na caused a reduction in VTG synthesis in juvenile goldfish （Carassius auratus） hepatocytes at concentrations 〉0.1μg/ml when co-exposure with 1μg/ml 17β-estradiol （E2）, making its anti-estrogenic activity approximately as potent as tamoxifen. Our results indicate that PCP-Na can act as negative modulators of estrogenic function in juvenile goldfish （Carassius auratus） hepatocytes.     

二氯异氰脲酸钠和三氯异氰脲酸对棕囊藻细胞去除的研究

研究了二氯异氰脲酸钠和三氯异氰脲酸对球形棕囊藻的灭杀和控制作用,及其投药时间对除藻效果的影响,并比较2种药剂的除藻效果,初步探讨了除藻机理.结果表明,这2种除藻剂可以缓释次氯酸,提高其稳定性,有较长的药效期.当有效氯浓度达到4.5mg/L时,2种药剂均能有效地控制和灭杀球形棕囊藻,具有高效、低毒的优点.有效氯浓度为5.0mg/L,藻细胞去除率在24h可达到90%以上.在等于和大于5.0mg/L有效氯浓度时,三氯异氰脲酸的除藻效果优于二氯异氰脲酸钠,通过对2种药剂除藻率的t检验进一步证实了这一结论.     

Variations of bacterial community structure in flooded paddy soil contaminated with herbicide quinclorac

The denaturing gradient gel electrophoresis (DGGE) method was applied to determine the relative genetic complexity of microbial communities in flooded paddy soil treated with herbicide quinclorac (3,7-dichloro-8-quinoline-carboylic acid). The results obtained showed a significant effect of quinclorac on the development of bacterial populations in soils contaminated with different concentrations of the herbicide at the early time after application. In general, however, the number of populations of the same soil sample treated with the same concentration of the quinclorac differed obviously with increasing incubation time within the early 8 weeks. The scale of differences in banding patterns-showed that the microbial community structures of the quinclorac-treated and non-quinclorac-treated soils were not significantly different after 21 weeks of incubation. Quantification, as demonstrated in this paper, was studied by establishing dose-response relationships. Significant pattern variations were quantified. Prominent DGGE bands were excised, cloned and sequenced to gain insight into the identities of predominant bacterial populations. The majority of DGGE band sequences were related to bacterial genera Clostridium, Sphingobacterium, Xanthomonas and Rhodococcus.     

连续刚构桥合拢状态分析

连续刚构桥作为超静定结构,合拢顺序的不同,必然影响到成桥结构的内力以及位移,尤其是多跨连续刚构桥.以云南某连续刚构桥为例,采用Midas/Civil软件建立仿真模型,考虑成桥阶段的累积位移以及内力,确定合理的合拢顺序,并以此考虑不同的合拢温度.以合拢顺序与合拢温度为变量,以竖向位移与结构次内力为目标,对结构进行多方位分...     

山西省循环流化床粉煤灰环境风险评估及管控建议

为服务山西省循环流化床粉煤灰环境管理,本文在对全省循环流化床锅炉粉煤灰调查的基础上,选取典型粉煤灰开展了物理化学性质及污染物赋存及浸出特征研究,开展了不同暴露途径的环境风险评估,提出了环境风险管控建议.结果表明,循环流化床粉煤灰堆存对土壤和地下水无致癌风险,但具有一定的危害性,需强化堆存规范性、着力源头减量、拓宽综合利...     

插板对正庚烷油池火燃烧行为的影响研究

海上石油泄漏常规的处理方法是原位燃烧,加速其燃烧并使其燃尽是降低其对生态环境影响的重要措施之一。以正庚烷为燃料,在油池内插入竖直铝板,研究不同高度铝板对池火燃烧行为的影响。结果表明,插板对池火燃烧速率以及火焰高度具有明显的增强作用,随着板的高度的增加,增强作用先增大后减小,当H_p/D(板高与油池直径之比)为3.5时,增强作用最大。火焰高度、板的温度、热通量以及燃烧速率的变化趋势一致,它们的临界点均在H_p/D=3.5附近。插板后燃烧速率增大主要是因为插板自身的热传导导致了燃料的核态沸腾,使燃料接受的热反馈增大,从而加快了燃料的蒸发,增大了燃烧速率。     

垃圾渗滤液组合工艺处理技术

针对垃圾渗滤液氨氮浓度高、COD浓度高、难降解物质含量高的特点,研究了MAP脱氮-生物处理-混凝组合工艺,考察了不同单元的作用及影响处理效果的因素。试验结果表明,高浓度的垃圾渗滤液经该优化组合工艺处理,污染物的去除率为COD97.5%、BOD599.2%、NH4-N87.2%、E26075.3%。除NH4-N外,其余指标均达到了生活垃圾填埋污染控制标准中规定的二级排放标准值。     

鱼体甲基汞的萃取富集和测定

研究了苯萃取和半胱氨酸滤纸富集再用苯洗脱的方法,使鱼肉中甲基汞得到富集和提取。建立了气相色谱测定甲基汞的方法并对所建立的方法进行评价。结果表明:加标回收率为87.10%～105.69%;变异系数为2.93%。符合要求,大量样品实验表明,该方法适用于痕量甲基汞的提取和分析。     

伊宁市空气中硫酸盐化速率与二氧化硫相关性研究

对伊宁市1999-2002年空气中硫酸盐化速率和二氧化硫监测值进行回归分析,结果表明,二者之间存在显著的线性正相关关系,但对各年度回归方程斜率和截距进行统计检验,则表现出显著的差异。     

浅谈磷石膏的综合利用

根据磷石膏作为湿法生产磷酸过程中的副产品,且含有少量的P2O5和氟等有害成分,是生产厂家面临的磷石膏的处理和再利用这一实际问题,论述了磷石膏的综合利用情况,为磷石膏的利用和开发提供了研究和参考的方向。