The effects of pesticides on greenhouse workers and their produced products

Pesticides are chemicals used in agriculture but are known to produce adverse effects on humans. In this study, blood activities of superoxide dismutase (SOD), catalase (CAT), aspartate transaminase (AST), alanine transaminase (ALT), gamma-glutamyl transaminase, lactate dehydrogenase (LDH), and levels of malondialdhyde (MDA) were determined in 44 greenhouse workers (24 tomato workers, 20 clove workers) and in tomato (24 samples) and clove (20 samples) products. At the end of this study, blood MDA levels and activities of ALT, AST, and LDH levels in greenhouse workers were significantly increased while SOD and CAT activity levels fell significantly. Pesticide residues were not found in agricultural worker blood. Malathion residues (average 0.8?ppm) were detected in tomatoes (8 samples) in these greenhouses. Data suggest that the health of the greenhouse workers may be adversely affected by pesticides.     

Does mating experience of male house crickets affect their behavior to subsequent females and female choice?

Male mating experience was shown to play an important role in settling conflicts between males; however, little is known about whether and how prior access to females influences male behavior during intersexual interactions and female choice itself. Here, I experimentally test this relationship in the house cricket (Acheta domesticus) by combining one-on-one interaction between the male and female with direct comparison of males by the female, but precluding aggression between males. I found that solitary males were more active during subsequent courtship displays than paired males, suggesting the detrimental effect of mating on courtship performance. At the same time, females spent significantly more time close to solitary males or playbacks of male's natural courtship songs, and responded positively to the condition of males, ignoring body size of males. In contrast, females responded similarly to computer-modified playbacks of courtship songs of solitary and paired males with standardized rate of phrases and amplitudes; however, when females were additionally allowed to contact with anesthetized males they spent more time close to bigger males, irrespective of the acoustic parameters of courtship songs. These results show that although females were able to differentiate between many behavioral and morphological characteristics of males, including voluntary and intrinsic ones, they preferred traits conditional upon the costliness of male's displays. In addition, mating experience appeared to be a crucial factor in the choice of a particular costly mating strategy by males.     

Effects of nanoparticles on Daphnia magna population dynamics

Spherical TiO₂ nanoparticles (npTiO₂) were prepared by controlled hydrolysis of tetraethoxy orthotitanate under a nitrogen atmosphere. ZnO nanoparticles (npZnO) were prepared using hydrothermal methods. The crystal structure, chemical, thermal and morphological properties of npZnO and npTiO₂ were characterised using Fourier Transform Infrared Spectrometer, enery-dispersive X-ray spectroscopy, X-ray diffraction, and scanning electron microscope techniques. The short- and long-term experiments were started with neonates taken from the same culture and laboratory condition. In the acute experiments, npTiO₂, npZnO, and cocktail concentrations were applied. 96h-LC₅₀ values were 1.8, 0.7, and 0.1?mg?L^?1, respectively (p?<?.05). For the chronic experiments, different npTiO₂ concentrations were performed. 21d-LC₅₀ value was 1.0?mgL^?1 (p?<?.05). Morphometry became progressively worse in concentrations of more than 1?mgL^?1 npTiO₂. Neonate and young individuals were more sensitive to death because of their low tolerance. This result was affected by population progeny and growth rates (p?<?.05). While control and 0.5?mgL^?1 npTiO₂ groups were determined as growing population, 1.5 and 2?mgL^?1 npTiO₂ groups had decreased population size as R₀ values. Consequently, the relationships between nanoparticle accumulation within Daphnia magna and its population structure and body morphometry for each concentration were important indicators. Its tolerance level to nanoparticles under laboratory conditions reflected its replacement and behaviour in the ecosystem.     

Hydrodynamics and contaminant transport on a degraded bed at a 90-degree channel confluence

Channel confluences at which two channels merge have an important effect on momentum exchange and contaminant diffusion in both natural rivers and artificial canals. In this study, a three-dimensional numerical model, which is based on the Reynolds Averaged Navier–Stokes equations and Reynolds Stress Turbulence model, is applied to simulate and compare flow patterns and contaminant transport processes for different bed morphologies. The results clearly show that the distribution of contaminant concentrations is mainly controlled by the shear layer and two counter-rotating helical cells, which in turn are affected by the discharge ratio and the bed morphology. As the discharge ratio increases, the shear flow moves to the outer bank and the counter-clockwise tributary helical cell caused by flow deflection is enlarged, leading the mixing happens near the outer bank and the mixing layer distorted. The bed morphology can induce shrinkage of the separation zone and increase of the clockwise main channel helical cell, which is initiated by the interaction between the tributary helical cell and the main channel flow and strengthened by the deep scour hole. The bed morphology can also affect the distortion direction of the mixing layer. Both a large discharge ratio and the bed morphology could lead to an increase in mixing intensity.     

Simultaneous Removal of Cationic and Anionic Dyes from Binary Solutions Using Carboxymethyl Chitosan Based IPN Type Resin

Journal of Polymers and the Environment - In this study, a novel bead form IPN type resin comprising poly (2-Dimethylaminoethyl) methacrylate and carboxymethyl chitosan networks with a high dye...     

Glass buildings on river banks as “polarized light traps” for mass-swarming polarotactic caddis flies

The caddis flies Hydropsyche pellucidula emerge at dusk from the river Danube and swarm around trees and bushes on the river bank. We document here that these aquatic insects can also be attracted en masse to the vertical glass surfaces of buildings on the river bank. The individuals lured to dark, vertical glass panes land, copulate, and remain on the glass for hours. Many of them are trapped by the partly open, tiltable windows. In laboratory choice experiments, we showed that ovipositing H. pellucidula are attracted to highly and horizontally polarized light stimulating their ventral eye region and, thus, have positive polarotaxis. In the field, we documented that highly polarizing vertical black glass surfaces are significantly more attractive to both female and male H. pellucidula than weakly polarizing white ones. Using video polarimetry, we measured the reflection-polarization characteristics of vertical glass surfaces of buildings where caddis flies swarmed. We propose that after its emergence from the river, H. pellucidula is attracted to buildings by their dark silhouettes and the glass-reflected, horizontally polarized light. After sunset, this attraction may be strengthened by positive phototaxis elicited by the buildings' lights. The novelty of this visual-ecological phenomenon is that the attraction of caddis flies to vertical glass surfaces has not been expected because vertical glass panes do not resemble the horizontal surface of waters from which these insects emerge and to which they must return to oviposit.     

Ventral polarization vision in tabanids: horseflies and deerflies (Diptera: Tabanidae) are attracted to horizontally polarized light

Adult tabanid flies (horseflies and deerflies) are terrestrial and lay their eggs onto marsh plants near bodies of fresh water because the larvae develop in water or mud. To know how tabanids locate their host animals, terrestrial rendezvous sites and egg-laying places would be very useful for control measures against them, because the hematophagous females are primary/secondary vectors of some severe animal/human diseases/parasites. Thus, in choice experiments performed in the field we studied the behavior of tabanids governed by linearly polarized light. We present here evidence for positive polarotaxis, i.e., attraction to horizontally polarized light stimulating the ventral eye region, in both males and females of 27 tabanid species. The novelty of our findings is that positive polarotaxis has been described earlier only in connection with the water detection of some aquatic insects ovipositing directly into water. A further particularity of our discovery is that in the order Diptera and among blood-sucking insects the studied tabanids are the first known species possessing ventral polarization vision and definite polarization-sensitive behavior with known functions. The polarotaxis in tabanid flies makes it possible to develop new optically luring traps being more efficient than the existing ones based on the attraction of tabanids by the intensity and/or color of reflected light.     

Estimation of end of life mobile phones generation: the case study of the Czech Republic

The volume of waste electrical and electronic equipment (WEEE) has been rapidly growing in recent years. In the European Union (EU), legislation promoting the collection and recycling of WEEE has been in force since the year 2003. Yet, both current and recently suggested collection targets for WEEE are completely ineffective when it comes to collection and recycling of small WEEE (s-WEEE), with mobile phones as a typical example. Mobile phones are the most sold EEE and at the same time one of appliances with the lowest collection rate. To improve this situation, it is necessary to assess the amount of generated end of life (EoL) mobile phones as precisely as possible. This paper presents a method of assessment of EoL mobile phones generation based on delay model. Within the scope of this paper, the method has been applied on the Czech Republic data. However, this method can be applied also to other EoL appliances in or outside the Czech Republic. Our results show that the average total lifespan of Czech mobile phones is surprisingly long, exactly 7.99 years. We impute long lifespan particularly to a storage time of EoL mobile phones at households, estimated to be 4.35 years. In the years 1990-2000, only 45 thousands of EoL mobile phones were generated in the Czech Republic, while in the years 2000-2010 the number grew to 6.5 million pieces and it is estimated that in the years 2010-2020 about 26.3 million pieces will be generated. Current European legislation sets targets on collection and recycling of WEEE in general, but no specific collection target for EoL mobile phone exists. In the year 2010 only about 3-6% of Czech EoL mobile phones were collected for recovery and recycling. If we make similar estimation using an estimated average EU value, then within the next 10 years about 1.3 billion of EoL mobile phones would be available for recycling in the EU. This amount contains about 31 tonnes of gold and 325 tonnes of silver. Since Europe is dependent on import of many raw materials, efficient recycling of EoL products could help reduce this dependence. To set a working system of collection, it will be necessary to set new and realistic collection targets.     

Degradation of PVC Containing Mixtures in the Presence of HCl Fixators

Degradation of a model polymer mixture (PVC/PS/PE) and a waste polymer mixture in the presence of HCl fixators (Red Mud, precipitated CaCO₃ and dolamite) was studied using thermal gravimetric analysis (TGA) and a cycled-spheres-reactor. The experiments in cycled-spheres reactor model were performed by stepwise pyrolysis. Liquid products and HCl from each step were collected separately. For the model polymer mixture, the precipitated CaCO₃ showed the best effect on the fixation of evolved HCl and the reduction of chlorine content in the liquid products whereas RM yielded the best result for the waste polymer mixture. In addition, using HCl fixator also affected the degradation of both types of polymer mixture, leading to the formation of more gaseous and less residue.     

Effects of synthetic pyrethroids and methidation on activities of some digestive enzymes in carp (Cyprinus carpio L.)

Abstract

The effects of pyrethroid pesticides (deltamethrin, permethrin and cypermethrin) and an organophosphate ester (methidation) on the activities of carp trypsin, α‐chymotrypsin, carboxypeptidase A and lipase were studied. The enzymes were isolated from the gastrointestinal tract and the effects of the pesticides were investigated during incubation for 5 min. The activity of trypsin was influenced only slightly by the presence of deltamethrin and methidation, whereas permethrin and cypermethrin caused significant inhibition. The pyrethroid pesticides at lower concentrations resulted in a slight activation of α‐chymotrypsin. Methidation inhibited the α‐chymotrypsin activity by about 20%. These pesticides modified the lipase activity to a lesser extent; the highest inhibition was measured with cypermethrin. The carboxypeptidase A activity was inhibited by both pyrethroid pesticides and methidation. The results suggest that these pesticides might interact with the active conformation of the studied hydrolytic enzymes, resulting in changes in their activities.