Prenatal diagnosis for dystrophia myotonica using the polymerase chain reaction

The polymerase chain reaction has been used to detect an abundant class of short repeat DNA families of the form (dC-dA)n.(dG-dT)n, known as microsatellites. These units are found throughout the human genome and have been characterized for several loci including APOC2 on chromosome 19ql2-ql3.2. The locus APOC2 is linked to the gene for dystrophia myotonica and a microsatellite within this locus was used to derive polymorphisms in a family to predict the inheritance of the disease. Chorionic villus sampling (CVS) was performed at 151/2 weeks' gestation. Following DNA extraction from the CVS material and parental blood samples, microsatellite analysis was carried out by the polymerase chain reaction.     

A participatory approach to the establishment of a baseline scenario for a reforestation Clean Development Mechanism project

This paper is part of a two-year study to investigate the feasibility of initiating a Clean Development Mechanism (CDM) project in an indigenous community of Eastern Panamá, Ipetí-Emberá. We use participatory mapping and matrices as well as household surveys to develop a land-use/land-cover baseline scenario and examine the role of local participation in assessing land-use change. In Ipetí, land-use change has not occurred in a linear way over the last decades, and our data unveils socio-economic factors as potential key drivers of change. The concordance that we observed between geographic information and individual and collective perceptions of land-use change substantiates the possibility of using local knowledge in the establishment of baseline data for CDM projects. Our calculations suggest that the total carbon (C) stocks in the Tierra Colectiva (TC) of Ipetí-Emberá in 2004 represents a 47% reduction from the estimated C stock at the onset of settlement in the early 1970’s. Results from the participatory assessments predict that, in 2024 and in absence of a CDM project, the C stocks will decline from 301,859 t C in 2004 to 155,730 t C, which constitutes a reduction of 52%. The scenario with CDM estimates C stocks of 305,853 t C for 2024, a value slightly superior to the 2004 value. In the TC there is ground to believe that cattle ranching is likely to become an ever more important activity as the population is young and growing and cannot easily move elsewhere. Forests tend to be cleared for cultivation while pastures are established on short fallows. Our baseline scenario underlines the potential for a CDM project to make a significant difference in the future C stocks of this landscape.     

Cellular and ultrastructural changes in the endoderm of the temperate sea anemone Anemonia viridis as a result of increased temperature

Exposure of the temperate sea anemone Anemonia viridis Forskål to increased seawater temperature (from 16 to 26°C) reduced the lysosomal latency of coelenterate tissues. Lysosomes in the mesenterial filaments of anemones were destabilised by increased temperature, with greater destabilisation in heat-shocked symbiotic anemones than in heat-shocked aposymbiotic anemones in the early stages of the experiment. Lysosomal enzyme activity in zooxanthellae from heat-shocked symbiotic anemones was associated with the algal membranes and the cytoplasm of degenerate algal cells. While the relationship between host coelenterate and symbiotic alga may confer many benefits under normal conditions, comparison of the responses of symbiotic and aposymbiotic anemones to heat shock suggests that there may be disadvantages for symbiotic anemones under stress.     

Permanent post-disaster housing in honduras: aspects of vulnerability to future disasters

To some degree it is unfair to evaluate a post-disaster housing program as to its effectiveness in decreasing vulnerability and preventing future disasters. As Burton states, "With rare exceptions, administrators and techniques have been trained to cope with disaster rather than to prevent it" [reference (2), p.197]. These were certainly not goals articulated by the agency responsible for constructing housing after Fifi. However, the authors feel that failure to evaluate specific projects by persons knowledgeable of the projects functioning, will only forestall the shift which Cuny calls for, "… from disaster response to disaster mitigation and prevention" [reference (4), p.123). In doing this we hope to add some specific case study data to the growing literature on disaster mitigation and prevention. Disaster vulnerability in Honduras is overwhelmingly related to flooding. More crucial than the materials and construction of housing is the issue of siting. If appropriately sited, houses made of bajarique, wood, or concrete block are able to withstand the heavy rains associated with a hurricane. Regarding the siting of the projects, the Honduras Project clearly has one positive and one negative accomplishment in the cases of Santa Rica and Flores, respectively. San Jose is less clear but is certainly a much safer site than those formerly occupied by the residents, in that there is no danger of flooding. The present site was not flooded during Fifi nor did it experience mudslides. However, the future is not so clear regarding the latter. Within the village proper a large amount of vegetation has been added which will tend to stabilise the soil on the steeper slopes. The streets, however, are seriously eroded and probably can not be maintained for vehicle usage, which does not pose a serious problem to the residents as none possess automobiles or trucks. One large gully bisects the village and receives run-off from the adjacent hills. It has been expanding, which would suggest that the slopes above the village could prove problematic in case of a Fifi-sized storm. Flores is located on a very poor site in reference to prevention and mitigation. It is located in a portion of the Sula Valley which is prone to flooding and, as mentioned before, was inundated by over 2m of water during hurricane Fifi. No prevention techniques were possible by NAEA/HEA and the houses were built on earthen mounds barely adequate to keep water out during the rainy season. The nearby dike which could possibly provide protection is non-functional due to poor maintainance. Given a storm of Fifi's magnitude, or possibly smaller, this site will again be flooded. Santa Rica is clearly well sited concerning flooding: it did not experience flooding during Fifi and is not flood prone. However, houses did experience some wall damage due to earthquakes following and associated with the Guatemalan quake of 1976. Due to the size and nature of the latter much "re-adjustment" occurred in the neighbouring fault system; however, damage to the houses was all superficial. We feel the residents were vocal about their concern due to the severity of the Guatemalan disaster and their lack of experience with concrete block houses. That the two sites (particularly Flores and partially San Jose) are vulnerable to future disasters cannot be considered solely the fault of an outside agency without local knowledge and understanding. In the engineering report issued during the construction it was explicitly stated that in Flores, "Future flooding remains a danger," [reference (6), p.49]. The future residents of Flores had gained access to the land from the National Agrarian Institute and were anxious to receive assistance in building homes. In fact, CARE, which had previously given these people tin roofing for houses, was threatening to take it back since the people had not yet started building. NAEA/HEA were responding to people in a rather desperate situation. But, on the other hand, they were responding to people who had been promised (not given) land by an agency of the Honduran national government which would be cognizant of the potential flooding at this site. Likewise, in San Jose, where mudslides and erosion remain a threat, the land was provided by a local government agency, the municipality. Although our goal in this discussion has not been to establish blame, we feel it imperative to mention the sequence of events that resulted in the questionable siting of Flores and San Jose. It is very easy and often accurate to place blame on outsiders who lack sophistication and knowledge about such matters. In this case local input did not result in post-disaster planning that is actually precautionary. This, we feel, illustrates the extreme complexity of cross-cultural aid, especially in the post-disaster period. It also points to the need for precautionary planning with reference to permanent post-disaster reconstruction.     

ECOSYSTEM DYNAMICS AND POLLUTION EFFECTS IN AN OZARK CAVE STREAM1

ABSTRACT: Subterranean ecosystems harbor globally rare fauna and important water resources, but ecological processes are poorly understood and are threatened by anthropogenic stresses. Ecosystem analyses were conducted from 1997 to 2000 in Cave Springs Cave, Arkansas, situated in a region of intensive land use, to determine the degree of habitat degradation and viability of endangered fauna. Organic matter budgeting quantified energy flux and documented the dominant input as dissolved organic matter and not gray bat guano (Myotis grisescens). Carbon/nitrogen stable isotope analyses described a trophic web of Ozark cavefish (Amblyopsis rosae) that primarily consumed cave isopods (Caecidotea stiladactyla), which in turn appeared to consume benthic matter originating from a complex mixture of soil, leaf litter, and anthropogenic wastes. Septic leachate, sewage sludge, and cow manure were suspected to augment the food web and were implicated in environmental degradation. Water, sediment, and animal tissue analyses detected excess nutrients, fecal bacteria, and toxic concentrations of metals. Community assemblage may have been altered: sensitive species‐grotto salamanders (Typhlotriton spelaeus) and stygobro‐mid amphipods—were not detected, while more resilient isopods flourished. Reduction of septic and agricultural waste inputs may be necessary to restore ecosystem dynamics in this cave ecosystem to its former undisturbed condition.     

Effect of Cross-Linking Waste Protein with Dialdehydes on Its Biodegradation Under Anaerobic Conditions

Most native polymers used in processing and application technologies are admittedly disposable from the environment in a biologic manner, but products possess low mechanical strength. One of the paths to increasing this attribute (if feasible) is their cross-linking, which may, however, affect their readiness to biodegradation. In the presented work this condition was observed on the example of waste protein (Hykol B) cross-linking by means of glutardialdehyde and glyoxal. Degree and course of cross-linking were determined through impedance spectroscopy. The objective of this work also was to obtain data for constructing a sensor capable of following the cross-linking course in real time, for potential industrial application of Hykol in continuous production. Impedance spectroscopy proved to be applicable even to this kind of material marked by considerable water content and exhibiting relatively high electric conductivity; so far it had been used only for materials of low conductivity. An aqueous environment inoculated with digested anaerobic sludge from a municipal wastewater treatment plant was selected for modeling anaerobic conditions. The relation was studied between cross-linking degree given by content of cross-linking agent (determined by impedance spectroscopy) and biodegradation degree under anaerobic conditions. It was confirmed that network density as given by quantity of added agent not only reduced breakdown degree but also slowed the course of the process. This fact is particularly obvious with cross-linking by means of glyoxal; network density is thus dependent on type of employed substance, which affect type and structure of created network. That not merely forms an obstacle during polymer swelling and dissolution but also prevents access of bacteria to source of metabolized organic carbon.