Radical Scavengers Suppress Low Frequency EMF Enhanced Proliferation in Cultured Cells and Stress Effects in Higher Plants

Summary In previous publications, we reported that sinusoidal varying magnetic fields (SVMF) modify the activity and dynamics of the malignancy marker adenosine deaminase, and enhance the proliferation of chick embryo fibroblasts (CEF). While the SVMF examined by us (50, 60 & 100 Hz / 0.06–0.7 mT) were all below kT, they may have the potential of altering chemical processes in which excited radicals are involved. We tested this hypothesis in two experimental systems: CEF in culture and Spirodela oligorrhiza (Lemnaceae) (a small aquatic plant, commonly known as Duckweed). CEF were exposed to SVMF of 100 Hz/0.7 mT for 24 h. The addition of the exogenous radical scavengers catalase, superoxide dismutase or vitamin E to the cells during exposure significantly suppressed enhancement of cell proliferation caused by the field (by 79, 67 and 82%, respectively, as evaluated by the MTT colorimetric assay). ¹⁵N NMR analysis of Duckweed plants fed by ¹⁵N-labeled ammonium chloride and exposed to SVMF at 60 and 100 Hz/0.7 mT for 24 h, revealed augmented alanine production. Alanine did not accumulate in the absence of SVMF. The addition of vitamin C, a radical scavenger, reduced alanine production by 82%. Exposure to SVMF resulted in specific metabolic stress effects in Duckweed plants and enhanced proliferation of CEF. In both cases, it is suggested that free radicals are involved.     

Harmonizing water management and social needs: a necessary condition for sustainable development. The case of Israel's coastal aquifer

This study focuses on the problem of most efficiently fulfilling the water requirements of society for sustainable water resources management. The goal is to coordinate effectively the social needs of the resident population with operational water resources management planning.The proposed approach consists of a pyramidal hierarchy of water resource management needs, similar to that suggested by psychologist Abraham Maslow for human social needs. The two pyramidal hierarchies can be simultaneously employed to delineate guidelines to synchronize planning for sustainable water resources development with the concerns and expectations of the resident population. In both hierarchies, higher level needs remain irrelevant and difficult to attain until lower level needs of the resident population have been fulfilled.Management planning measures employed with regard to Israel's coastal aquifer have been used to illustrate this approach. Observation of Israel's experience indicates markedly reduced effectiveness where such measures have failed to be properly synchronised with societal needs. Conversely, where hydrological management measures were successfully synchronized with societal concerns, increased efficiency towards attaining sustainable groundwater management was evident.     

Tracer diffusion from a horizontal fracture into the surrounding matrix: measurement by computed tomography

The vertical diffusion of NaI solution from a horizontal fracture into and within the surrounding matrix was tracked and quantified over time using an artificially fractured chalk core (30x5 cm) and a second-generation X-ray computed tomography (CT) scanner. The different tracer-penetration distances imaged in the matrix above and below the horizontal fracture are indicative of a greater tracer mass penetrating into the lower matrix. The enhanced transport in the matrix below the fracture was related to the Rayleigh-Darcy instability induced by the density differences between the heavier tracer solution in the fracture (1.038) and the distilled water that had initially resided in the matrix. Our observations suggest that below the fracture, the tracer is propagated by an advection-diffusion process that is characterized by both higher rates and higher concentrations relative to its propagation by diffusion above the fracture. The experimental results suggest that the prediction of contaminant migration in a rock intersected by both vertical and horizontal (e.g. along bedding planes) fractures is difficult because of density effects that result in different solute-penetration rates.     

Data search and environmental hazard assessment for post-seveso chemicals

After the serious accident with a chemical industrial plant at Seveso (Italy) in 1976, the Commission of the European Communities has made a directive on major accidents, usually called the Post-Seveso Guideline. In its annex III, a list of 178 substances is given for which potentially dangerous volumes in case of an accident are defined on the basis of human health criteria. For the implementation of environmental criteria, information on toxicity to aquatic organisms, dilution, and physical behavior in water is needed. A literature search for relevant data on these 178 substances revealed aquatic toxicity data for 116 substances, but only for 87 substances sufficient data were available to make a hazard assessment. As an example, the River Rhine was used to calculate the critical quantities that, after a sudden discharge in the Swiss part, could cause damage at the Dutch part some 800 km downstream. In the absence of a common opinion on the criteria for being a major accident and a serious environmental hazard, the impact of different criteria quantities is discussed. If, for example, in the whole River Rhine a mortality of 5% of the aquatic species is chosen as the criterion for damage to the ecosystem, the critical quantities as mentioned in the EEC directive have to be lowered drastically for the majority of the substances. For 18 substances it could be shown that release of the mentioned quantities would be catastrophic for aquatic life in the whole river.     

Urban hinterlands—the case of an Israeli town ecological footprint

Modern urban life is characterized by the consumption of materials and energy, which are imported from all over the world and discharge waste that in many cases has a negative impact on ecosystems far from the cities in which they consumed. Indeed, cities cannot survive without worldwide hinterlands for resources and emissions sequestration. The ecological footprint (EF) concept provides valuable insights into the human appropriation of resources relative to earth’s carrying capacity, and therefore it enables us to compare human demands with nature’s supply and provides an indicator of human ecological sustainability. An attempt was made to calculate the EF of Ra’anana, Israel as a case study, to compare the EF-value with the expected for ecological sustainability and to emphasize the dependence on overseas ecosystems. Ra’anana, a town of 67,300 inhabitants in the year 2002, is considered a ‘dormitory town’ with a high quality of life. The EF was calculated using mainly the component method. The calculated EF for Ra’anana is 4.0 ha/resident which means that the required hinterland, located all over the world, is nearly 180 times the size of the town. The town’s EF is twice the value expected for sustainability on a global scale. We draw several scenarios in order to reduce the EF. On a national basis as well as with the town case study, electric energy, food and waste can be reduced and in turn would have a dramatic impact on the EF.

Lipogenesis in the intact coral Pocillopora capitata and its isolated zooxanthellae: Evidence for a light-driven carbon cycle between symbiont and host

Surface tissue of the reef coral Pocillopora capitata contained approximately 34% lipid on a dry weight basis. Of this, 75% was storage lipid (wax ester and triglyceride) and 25% structural (phospholipid, galactolipid, etc.). Based on chlorophyll a: lipid ratios of intact coral and isolated zooxanthellae, it was determined that over 90% of the storage lipid resided in the host tissue. One half of the structural lipids was found in the host and the other in the symbiotic algae. Gentle fractionation of coral tissue indicated that zooxanthellae possessed less than 14% of the total coral protein. Coral tips and isolated zooxanthellae were incubated with sodium acetate-1-¹⁴C in light and dark to obtain lipogenic rates and proportions of fatty acids and lipid classes synthesized. The rate of lipid synthesis from acetate-1-¹⁴C by intact coral was stimulated three-fold in the light (1200 lux), which indicated that the majority of coral lipogenesis occurred in the zooxanthellae. Intact coral triglycerides contained ca. 68% of the ¹⁴C-activity and wax esters ca. 21%. Zooxanthellae isolated by the Water Pik technique synthesized negligible amounts of wax ester, which implied that wax ester synthesis was a property of the animal tissue. Isolated zooxanthellae and intact coral synthesized identical triglyceride fatty acids from acetate-1-¹⁴C. This study provides evidence for a carbon cycle between host and symbiont whereby the zooxanthellae take up host-derived carbon (probably in the form of acetate), synthesize fatty acids using their photosynthetically derived energy, and return the lipid to the host where it appears as wax ester and triglyceride.     

Production of sexuals in a fission-performing ant: dual effects of queen pheromones and colony size

Models based on the kin selection theory predict that in social hymenopterans, queens may favor a lower investment in the production of sexuals than workers. However, in perennial colonies, this conflict may be tuned down by colony-level selection because of the trade off between colony survival and reproductive allocation. In this study, we present a survey of sexual production in colonies of Aphaenogaster senilis, a common species of ant in the Iberian Peninsula. Similar to most species that reproduce by fission, males were found in large excess compared to gynes (172:1). Sexuals were more likely to be found in queenless than in queenright (QR) field colonies. However, we also found a few gynes and numerous males in very large QR colonies. We compared these data with those available in the literature for A. rudis, a congeneric species from North America that has independent colony founding. The sex ratio in this species was only five males for each female, and sexuals were mostly found in QR nests, irrespective of colony size. We confirmed queen inhibition of sexual production in A. senilis in laboratory experiments and provide evidence that this inhibition is mediated by a nonvolatile pheromone. To seek the potential source of such a queen pheromone, we analyzed the secretions of two conspicuous exocrine glands, the Dufour’s and postpharyngeal glands (DG and PPG, respectively) in both queens and workers. Both secretions were composed of hydrocarbons, but that of DG also contained small quantities of tetradecanal and hexadecanal. The hydrocarbon profile of the DG and PPG showed notable caste specificity suggesting a role in caste-related behavior. The PPG secretions also differed between colonies suggesting its role in colony-level recognition. We suggest that in A. senilis, there are two modes of colony fission: First, in very large colonies, gynes are produced, probably because of the dilution of the queen pheromone, and consequently one or more gynes leave the mother colony with workers and brood to found a new nest. This is beneficial at the colony level because it avoids the production of costly sexuals in small colonies. However, because the queen and workers have different optima for sexual production, we hypothesize that queens tend to overproduce the pheromone to delay their production. This in turn may drive workers to leave the mother colony during nest relocation and to produce sexuals once they are away from the queen’s influence, creating a second mode of colony fission.     

A Review of WTA/WTP Studies

Willingness to accept (WTA) is usually substantially higher than willingness to pay (WTP). These constructs have been studied for roughly 30 years and with a wide variety of goods. This paper reviews those studies. We find that the less the good is like an “ordinary market good,” the higher is the ratio. The ratio is highest for non-market goods, next highest for ordinary private goods, and lowest for experiments involving forms of money. A generalization of this pattern holds even when we account for differences in survey design: ordinary goods have lower ratios than non-ordinary ones. We also find that ratios in real experiments are not significantly different from hypothetical experiments and that incentive-compatible elicitation yields higher ratios.     

Biologie du chinchard de la Mer Noire (Trachurus meditarraneus ponticus)

A study has been made regarding the action of the hydrocortisone, testosterone and 17β-estradiol on isolated liver and spleen tissue homogenates or slices of male and female adults of the fish Trachurus mediterraneus ponticus. The results showed a decrease of the oxygen uptake and of the cytochrome oxydase and succinic dehydrogenase activity of the liver homogenate after hydrocortisone administration, while this hormone increased significantly the lactic acid production in both tissues. Testosterone acted in an opposite manner to hydrocortisone by increasing the intensity of oxygen consumption and the succinic dehydrogenase activity, but decreased the lactic acid production of the liver and spleen tissue preparations. Estradiol decreased the oxygen consumption of the liver, the cytochrome oxydase activity and the lactic acid production in both tissues of female Trachurus mediterraneus ponticus.