Composite Biofilms grown in Acidic Mining Lakes and assessed by Electron Microscopy and Molecular Techniques

Microbial consortia of composite biofilms, grown in surface water of acidicmining lakes near Lauchhammer, Germany, were investigated. The red-brown colored lake water was acidic (pH 2.5), had high concentrations of Fe(III), Al(III), and sulphate and low concentrations of dissolved organic matter. As a result the abundance of bacteria in the lake is with 10⁴ cells mL^-1 rather low. One input of organic material into the lake are autumnal leaves from trees, growing in the lakeside area. From aliquots of unfixed birch leave biofilms the 16S rRNA genes were amplified by PCR and community fingerprints were determined by single-strand conformation polymorphism (SSCP) analysis. Specific bands within the fingerprints were extracted from SSCP gels and sequenced for the taxonomical affiliation.These results were compared with those from the second type of biofilms which were grown on sterile substrata, floating submersed in surface waters of the lakes. By excising the bands from the gel and sequencing the individual bands bacterial taxa, common to both types of biofilms, were found but also some, which were only present in one type of biofilm. Ultrathin sectioned biofilms often showed bacteria associated with electron dense particles as main inorganic constituents. Elemental microanalysis by energy dispersive X-ray analysis (EDX) revealed them to contain iron, sulfur and oxygen as main elemental fractions and electron diffraction ring pattern analysis classified them to be schwertmannite. These bacteria and their interactions with each other as well as with the inorganic minerals formed in this lake generally is of great interest, in order to use these results for bioremediation applications.     

Peregrine falcon egg pollutants

The role of the peregrine falcon (Falco peregrinus) as a bioindicator for the accumulation of organohalogen compounds and other persistent organic pollutants has been established in field studies. Biometric indices for damage caused by pollutants such as the shell thickness and the shell index were determined and the egg contents were analyzed for various pollutants by gas chromatography/mass spectrometry. A wide range of chemically activated luciferase expression (CALUX®) bioassays were performed on subsamples of the eggs.

The following organohalogen compounds were found in the eggs of 2009: dichlorodiphenyltrichloroethane (DDT) and dichlorodiphenyldichloroethane, heptachlor epoxide, hexachlorobenzene, dieldrin, hexachlorocyclohexane, polychlorobiphenyls and polychlorodibenzodioxins and polychlorodibenzofurans, polybromodiphenyl ethers, hexabromocyclododecane, tetrabromobisphenyl A, perfluoro compounds, and mercury. The DDT metabolite, dichlorodiphenyldichloroethene (DDE) (11,800 ng/g dry matter), was found to be the most highly concentrated egg contaminant followed by 2,2′,4,4′,5,5′-hexachlorobiphenyl (3800 ng/g). After a past general drop in pollution level, most egg contaminants presently plateau at levels that may still exceed limit values in foods of animal origin (DDE) or even toxicological thresholds (polychlorobiphenyls + polychlorodibenzodioxins, polybromodiphenyl ethers, perfluorooctanesulfonate, methylmercury).

Accumulation of DDE could be shown in peregrine falcon eggs from the uplands of Southwest Germany with elevations up to 1500 m, presumably due to its global distribution and its cold condensation in higher altitudes. In contrast, the concentration of polychlorobiphenyls in falcon eggs decreases with elevation, indicating that these pollutants originate mainly from conurbations and local industrial sites.

Significant negative correlations were found between both shell index and thickness and the concentration of Hg. A deleterious effect is also evident from a no-observed-adverse-effect level of 120 ng MeHg per gram egg determined by other authors in chronic feeding studies with ibises, which resulted in decreased egg productivity and male homosexual nesting and courtship behavior. The average Hg concentration in the peregrine falcon eggs from 2009 is almost four times higher than this level. MeHg accounted for 82.5% of the Hg present in the eggs of 2009 and 2010.

The cell test DR CALUX® for screening of dioxin-like activities can be used to detect not only the 29 regulated dioxin-like substances but also many other persistent organic pollutants with dioxin-like potencies, such as mixed halogenated dioxins/biphenyls. In our case, the results of bioanalytical screening methods showed no additional effect of other compounds with dioxin-like activity.

Ninety-three out of 177 analytes sought could be detected in the eggs. Chlorinated paraffins, organotin compounds, some pesticides that are still in use, and phthalic esters with the exception of traces of diethylhexyl phthalate could be excluded. All pollutants found in the eggs belong to substance classes banned by the Stockholm and Minamata Conventions.     

Distance from the queen affects workers’ selfish behaviour in the honeybee (A. mellifera) colony

It has been postulated that spatial organization of the colony, in addition to biological and social factors, influences colony life in honeybees. In this study, we examine the influence of workers’ distance from the queen on their reproductive, pheromonal and behavioural characteristics. Our results demonstrate that with increasing distance from the queen, workers increasingly develop behavioural and reproductive traits characteristic of queenlessness, which are presumably the result of significant impairment in queen pheromone transmission. Having contemplated the alternative possibilities of inadequate transmission of queen signal and voluntary escape from queen control, we concluded, based on the behavioural and physiological data, that the former is far more probable than the latter. The dose-dependent manner of the queen pheromone action and its differential influence on various worker characteristics are discussed.     

Land use and socioeconomic influences on a vulnerable freshwater resource in northern New England, United States

Land use and cover conversions as well as climatic factors drive current and future threats to freshwater systems. Research from the United States and across the globe has focused on already threatened and degraded freshwater systems, whose recovery requires significant investments. Attention must also be directed to monitoring freshwater systems that may appear robust, yet are likely to face enhanced vulnerabilities in the future due to climate and land use and cover changes. Such proactive monitoring can help identify problems early and provide potential solutions. In this study, we consider the case of Sebago Lake and its watershed in southern Maine; a region that has experienced significant population growth and development activity. Land use, socioeconomic change and water quality trends are monitored over a 20-year period using Landsat imagery, census, water quality and precipitation data. Our results indicate that Developed Land within the watershed has increased from 5.4 % of the total land area in 1987 to 8.9 % in 2009 with associated increases in population and housing activity. Sebago Lake’s water quality indicators from 1990 to 2010 show a directional trend concomitant with this change. The increase in Developed Land is likely to place additional pressures on water quality in the future. The analysis also indicates that precipitation trends play an important role in water quality variability for Sebago Lake. Predicted changes to climatic factors including enhanced spring time precipitation or earlier ice-out conditions combined with further land use change may play an influential role in determining water quality. The analysis highlights emerging areas of concern and reiterates the essential role of proactively monitoring vulnerable systems to help mitigate future threats.     

Regulation of reproduction by dominant workers in bumblebee (Bombus terrestris) queenright colonies

The mechanisms of regulating worker reproduction in bumblebees were studied by direct behavioral observations and by measuring ovarian development and juvenile hormone (JH) biosynthesis rates in workers under different social conditions. Workers in the last stage of Bombus terrestris colony development (the competition phase) had the lowest ovarian development and JH biosynthesis rates. Callows introduced into colonies immediately after queen removal (dequeened colonies) demonstrated a significant increase in ovarian development before, but not during, the competition phase. These findings differ from the higher ovarian development in colonies during the competition phase predicted by the prevailing hypothesis that worker reproduction starts in response to a decrease in queen inhibition. Reproduction of callows housed with dominant workers in small queenless groups was inhibited as in queenright colonies. This suggests that the reduced ovarian development and JH biosynthesis rates observed in dequeened and normally developing colonies during the competition phase also reflect inhibition by dominant workers. Thus, two distinct stages of inhibition of reproduction seem to exist: (1) before the competition phase, when the queen slows down worker ovarian development and prevents oviposition; (2) during the competition phase, when dominant workers inhibit ovarian development of other workers. Between these stages there seems to be a temporal “window” of enhanced worker reproductive development. The queen's typical switch to haploid egg production was not associated with changes in worker ovarian development or JH biosynthesis rates. These findings suggest that regulation of worker reproduction in B. terrestris is not determined by simple changes in the queen's inhibition capacity or by the sex of offspring and that the worker's role is more important than previously believed. Received: 18 March 1998 / Accepted after revision: 18 July 1998     

Summer Temperature Patterns in Headwater Streams of the Oregon Coast Range1

Abstract: Cool summertime stream temperature is an important component of high quality aquatic habitat in Oregon coastal streams. Within the Oregon Coast Range, small headwater streams make up a majority of the stream network; yet, little information is available on temperature patterns and the longitudinal variability for these streams. In this paper we describe preharvest spatial and temporal patterns in summer stream temperature for small streams of the Oregon Coast Range in forests managed for timber production. We also explore relationships between stream and riparian attributes and observed stream temperature conditions and patterns. Summer stream temperature, channel, and riparian data were collected on 36 headwater streams in 2002, 2003, and 2004. Mean stream temperatures were consistent among summers and generally warmed in a downstream direction. However, longitudinal trends in maximum temperatures were more variable. At the reach scale of 0.5‐1.7 km, maximum temperatures increased in 17 streams, decreased in seven streams and did not change in three reaches. At the subreach scale (0.1‐1.5 km), maximum temperatures increased in 28 subreaches, decreased in 14, and did not change in 12 subreaches. Models of increasing temperature in a downstream direction may oversimplify fine‐scale patterns in small streams. Stream and riparian attributes that correlated with observed temperature patterns included cover, channel substrate, channel gradient, instream wood jam volume, riparian stand density, and geology type. Longitudinal patterns of stream temperature are an important consideration for background characterization of water quality. Studies attempting to evaluate stream temperature response to timber harvest or other modifications should quantify variability in longitudinal patterns of stream temperature prior to logging.     

Cleaner pathways of hydrogen,carbon nano-materials and metals production via solar thermal processing

This paper describes various solar thermochemical processes for the production of hydrogen, carbon nano particles, industrial grade carbon black, and metals with substantially reduced CO₂ emission footprint. The paper introduces an innovative approach of a three-dimensional volumetric production of carbon nano particles via thermal cracking of methane gained by carbon seeding as an alternative to the existing two dimensional modes. The paper also describes an alternative pathway for hydrogen production via three consecutive solar thermochemical processes, namely, solar cracking of methane, solar carbo-reduction of ZnO and CO reduction of CdO, providing long term storage of solar energy. Finally, the paper provides an example solar windowed reactor for clean production of hydrogen, and it presents numerical analysis of the solar reactor based on computational fluid dynamics results, simulating one of the major problems with natural gas cracking in solar reactors, namely, carbon contamination of the transparent window and clogging of the reactor.     

Application of game theory for a groundwater conflict in Mexico

Exploitation of scarce water resources, particularly in areas of high demand, inevitably produces conflict among disparate stakeholders, each of whom may have their own set of priorities. In order to arrive at a socially acceptable compromise, the decision-makers should seek an optimal trade-off between conflicting objectives that reflect the priorities of the various stakeholders. In this study, game theory was applied to a multiobjective conflict problem for the Alto Rio Lerma Irrigation District, located in the state of Guanajuato in Mexico, where economic benefits from agricultural production should be balanced with associated negative environmental impacts. The short period of rainfall in this area, combined with high groundwater withdrawals from irrigation wells, has produced severe aquifer overdraft. In addition, current agricultural practices of applying high loads of fertilizers and pesticides have contaminated regions of the aquifer. The net economic benefit to this agricultural region in the short-term lies with increasing crop yields, which requires large pumping extractions for irrigation as well as high chemical loading. In the longer term, this can produce economic loss due to higher pumping costs (i.e., higher lift requirements), or even loss of the aquifer as a viable source of water. Negative environmental impacts include continued diminishment of groundwater quality, and declining groundwater levels in the basin, which can damage surface water systems that support environmental habitats. The two primary stakeholders or players, the farmers in the irrigation district and the community at large, must find an optimal balance between positive economic benefits and negative environmental impacts. In this paper, game theory was applied to find the optimal solution between the two conflicting objectives among 12 alternative groundwater extraction scenarios. Different attributes were used to quantify the benefits and costs of the two objectives, and, following generation of the Pareto frontier or trade-off curve, four conflict resolution methods were then applied.