Matching spatial distributions of the sea star Echinaster sepositus and crustose coralline algae in shallow rocky Mediterranean communities

Understanding why a species is present in a particular location and the consequences of its presence is complex but necessary to identify the mechanisms that generate and maintain ecological diversity. The common sea star Echinaster sepositus can be either very abundant or non-existing in nearby localities of the western Mediterranean. Yet, the factors that shape its distribution and the impact of the sea star on natural communities remain uninvestigated. Here, we quantified multiple biotic and abiotic factors that may affect the distribution of E. sepositus and tested whether this sea star can shape the organization of the community it inhabits. Our results showed that the distribution of this sea star was highly contagious and positively correlated with the abundance and distribution of crustose coralline algae from tens of meters to tens of kilometers. Despite significant differences in community composition between localities with high or low abundance of the sea star, experimental addition of E. sepositus to natural communities failed to shift the composition of the algal community in 4 months. Overall, our results suggest that within habitat variability in the abundance of crustose coralline algae may explain the abundance of E. sepositus at multiple geographic scales, emphasizing the need to investigate small-scale processes at larger geographic scales.     

Investigation of arsenic speciation on drinking water treatment media utilizing automated sequential continuous flow extraction with IC-ICP-MS detection

Three treatment media, used for the removal of arsenic from drinking water, were sequentially extracted using 10 mM MgCl2(pH 8), 10 mM NaH2PO4(pH 7) followed by 10 mM (NH4)2C2O4(pH 3). The media were extracted using an on-line automated continuous extraction system which allowed the arsenic in each of the extraction fluids to be speciated on-line using IC-ICP-MS. The 10 mM MgCl2 preferentially extracted As(III) from each of the media. The percentage of the arsenic extracted by the MgCl2, relative to a HNO3/H2O2 digestion of the media, ranged from 0.1-2.3% for the three solids. The next sequential extraction fluid, 10 mM NaH2PO4, extracted some of the residual As(III) remaining on each of the media but the predominant species extracted was As(V). The 10 mM NaH2PO4 extracted 15.3 to 42.8% of the total arsenic relative to a total digested concentration for each of the media. The As(III) and As(V) stability studies conducted in these two extraction fluids indicated that conversion between As(III) and As(V) was not significant for the short extraction fluid sample contact time associated with the on-line continuous flow extraction cell. Finally, the 10 mM (NH4)2C2O4 extraction fluid was utilized in an off-line analysis mode because the Fe and As concentrations extracted from the media were not compatible with direct ICP-MS detection. The (NH4)2C2O4 extracted 2.9-29% As(III) for all three media and caused an oxidation of As(III) to As(V) during the extraction period for one of the three media. The sum of the arsenic from each of the three extraction fluids represented 92%, 44% and 53% of the available total arsenic for the three media, respectively. The speciation results for each media were obtained by adding all the speciation results from all three extraction fluids together and the resulting distribution of As(III)/As(V) compared well with the speciation results obtained via XANES.     

Rapid prenatal diagnosis of sickle cell diseases using oligonucleotide ligation assay coupled with laser-induced capillary fluorescence detection

Prenatal diagnosis of sickle cell diseases has been available for several years, and our laboratory has performed over 1000 prenatal diagnoses. However, currently available techniques are labor-intensive and time-consuming, and thus the diagnosis is delayed, making the mother's decision difficult. We describe a rapid, high-throughput technique based on the ligation assay coupled with automated capillary fluorescence detection. This new approach allows the diagnosis of both Hgb S and Hgb C to be available in a few hours. We have utilized this technique in 30 prenatal diagnoses and found it to be in complete agreement with the standard diagnoses. Copyright © 2002 John Wiley & Sons, Ltd.     

STREAM CHANNEL ADJUSTMENTS FOLLOWING ELIMINATION OF CAVFLE GRAZING1

ABSTRACT: Cattle grazing practices in the western United States have contributed to widespread riparian degradation resulting in unstable channel morphologies and the loss of fish habitat. Because of prolonged disturbance, numerous riparian areas on both public and private lands have been fenced to exclude cattle in order to promote vegetation establishment and riparian improvement. We selected four gravel-bedded, steep alluvial streams in eastern Oregon with cattle exclosures greater than 14 years old for an analysis of geomorphic adjustments following the removal of cattle grazing. We compare channels inside exclosures and in adjacent grazed reaches to identify the salient stream channel properties that respond to the removal of riparian stresses and to document the magnitude of these changes. Results indicate that significant changes occur, with reductions in bankfull dimensions and increases in pool area being the most common and identifiable changes. At all four sites, bankfull widths are narrower by 10 to 20 percent, and the percentage of channel area occupied by pools is higher in the exclosure by 8 to 15 percent. The increase in pool area is primarily offset by a reduction in the percent glide area. Not all of the channel properties demonstrate adjustment, indicating that perhaps 14 years is an insufficient duration for these variables to adjust.     

Drug and drink driving by university students: an exploration of the influence of attitudes

The present study aimed to gather information on drug and alcohol use, the prevalence of drink and drug driving and attitudes to both in an undergraduate university cohort (n = 275). Further, the study aimed to contribute information to aid intervention program development by determining predictors of drink and drug driving behavior. The study examines both drug and alcohol use and the influence of attitudes toward driving while under the influence of alcohol and/or drugs. Results indicated that significant proportions of university students are engaging in the risk-taking behavior of driving under the influence of drugs (25%), alcohol (14%), or both (8%). The results suggest that attitudes toward driving under the influence of alcohol and/or drugs are a strong predictor of engaging in the behavior. Peer influence emerged as a major aspect of attitudes. The results of this study provide an important beginning to understanding drink and drug driving and the influence of attitudes in the university student body.     

The genesis of GDL

This paper discusses the early research that lead to graduated driver licensing, some of the educational principals on which it is based, obstacles to its acceptance, and some of the early efforts in the U.S. and elsewhere. EARLY RESEARCH: The research underlying the concept of graduated driver licensing was a 1971 North Carolina study that identified the overrepresentation of young drivers in crashes at night and when another young person was the right front passenger. EDUCATIONAL PRINCIPALS: Efforts to reduce the risk to young novice drivers applied what was known about learning. The concepts included distributed learning (i.e., over time) and progressing from simple to complex skills. A PROPOSAL: The proposed graduated licensing system based on learning principals included (a) initial experience under low risk conditions, (b) extended supervised practice, (c) gradual move to more complex conditions, and (d) harsher penalties for deliberate risk-taking. OBSTACLES: There were several most common objections raised against graduated licensing. Raising the licensing age decreased mobility. Some young drivers were "good" drivers. Enforcement is difficult. Fear of parental objections. Parents are not driver educators and some young people do not have an available parent. Administrative costs are too high. ACCEPTANCE: Driver educators were the first to see the benefits of a graduated system in the 1970s and 1980s. Toronto nearly adopted a graduated system in 1976. New Zealand was the first to adopt a graduated licensing system in 1984. Michigan in 1997 was the first state to require parental certification of extended supervised driving practice.     

Quantitative determination of arsenobetaine,the major water-soluble arsenical in three species of crab,using high pressure liquid chromatography and an inductively coupled argon plasma emission spectrometer as the arsenic-specific detector

The major water-soluble arsenic compound in the Alaskan king crab, the Alaskan snow crab, and the Dungeness crab was identified as arsenobetaine by HPLC/ICP analysis. This technique is suitable for the identification and quantitative determination of naturally occurring organic arsenic compounds in purified and partially purified extracts from biota.     

Patterns of Aquatic Species Imperilment in the Southern Appalachians: An Evaluation of Regional Databases

/ For regional analyses of species imperilment patterns, data on species distributions are available from the U.S. Fish and Wildlife Service and from the state heritage programs. We compared these two different databases as sources of best available information for regional analyses of patterns of aquatic species imperilment for 132 counties in the southern Appalachians and examined patterns produced from the databases. The heritage program database contained information about a greater number of imperiled species because species need not be federally listed as threatened or endangered to be included in this database. In the southern Appalachians, about half of imperiled molluscs and about one-fourth of imperiled fish were listed as threatened or endangered; much smaller proportions of other taxonomic groups were federally listed. Most threatened and endangered species appeared on both lists, but for about 40% of the species inconsistencies exist, notably a lack of recent records in the heritage program dataset. Numbers of species in each county were significantly different between the two datasets for Georgia, Tennessee, and Virginia, where the largest number of threatened and endangered species reside. Nevertheless, some counties always appeared as centers of imperilment, and the general spatial patterns of imperilment were similar.