Susceptibility of Eucalyptus globulus to Mnesampela privata defoliation in relation to a specific foliar wax compound

Summary. Increased abundance of several aliphatic benzyl and phenylethyl alkanoates were previously associated with reduced defoliation of Eucalyptus globulus Labill. by the autumn gum moth (Mnesampela privata Guenée) in two clonally replicated F₂ families. We examine the robustness of this association by comparing the abundance of the implicated foliar wax compounds with defoliation levels in three plantation sites and a genetics trial in northern Tasmania. At all locations, the aliphatic alkanoate, benzyl n-tetracosanoate (C₂₄), was found in significantly higher abundance in the foliar wax of E. globulus trees that had displayed low M. privata damage (designated resistant) compared to trees that had displayed high damage (designated susceptible). Further, when sites were combined, benzyl n-tetracosanoate (C₂₄) accounted for 16 % of variation in field defoliation. Field defoliation was also positively correlated to M. privata oviposition both in the field and on foliage sprigs in a cage. In the cage bioassay, oviposition accounted for 20 % of variation in field defoliation making it a better predictor of tree defoliation in the field than either field oviposition, which accounted for 9 % of variation, or screening foliage for the abundance of benzyl n-tetracosanoate (C₂₄). Despite both benzyl n-tetracosanoate (C₂₄) and oviposition being related to field defoliation, there was no statistically significant relationship between the abundance of benzyl n-tetracosanoate (C₂₄) and oviposition in the field or cage bioassay. Further work is therefore required to determine if increased levels of this compound in the foliar wax operates as a direct deterrent to M. privataoviposition.     

Association patterns and kinship in female Indo-Pacific bottlenose dolphins (Tursiops aduncus) of southeastern Australia

Kinship has been shown to be an important correlate of group membership and associations among many female mammals. In this study, we investigate association patterns in female Indo-Pacific bottlenose dolphins (Tursiops aduncus) inhabiting an embayment in southeastern Australia. We combine the behavioral data with microsatellite DNA and mitochondrial DNA data to test the hypotheses that genetic relatedness and maternal kinship correlate with associations and social clusters. Mean association between females was not significantly different from a random mean, but the standard deviation was significantly higher than a random standard deviation, indicating the presence of nonrandom associates in the dataset. A neighbor-joining tree, based on the distance of associations between females, identified four main social clusters in the area. Mean genetic relatedness between pairs of frequent female associates was significantly higher than that between pairs of infrequent associates. There was also a significant correlation between mtDNA haplotype sharing and the degree of female association. However, the mean genetic relatedness of female pairs within and between social clusters and the proportion of female pairs with the same and different mtDNA haplotypes within and between clusters were not significantly different. This study demonstrates that kinship correlates with associations among female bottlenose dolphins, but that kinship relations are not necessarily a prerequisite for membership in social clusters. We hypothesize that different forces acting on female bottlenose dolphin sociality appear to promote the formation of flexible groups which include both kin and nonkin.     

Simulated marine respirometry of biodegradable polymers

Eleven microorganisms were isolated from several temperate marine locations in the northeast Altantic coast of the United States and one tropical location in the Pacific Ocean (Hawaii) for the purpose of developing a rapid and accurate method of screening biodegradable materials for their susceptibility to mineralization. The materials evaluated in this study included chemically modified starch, amylose and pullulan, poly(3-hydroxybutyrate-co-valerate), (PHB/V), cellulose acetate, and a modified lignin/styrene. Some of the soluble, unmodified, biologically produced substrates such as starch, pullulan, and amylose mineralized rapidly. In general, the synthetic, insoluble polymers and the chemically modified polymers, such as acetylated and chlorinated amylose and pullulan, mineralized more slowly, although the ultimate mineralization of some of the substituted polysaccharides equaled or exceeded that of the unmodified substrate. The insoluble bacterial polyester, PHB/V, degraded rapidly after a short induction period. Initial respiration rate data, in general, could not be used as a predictor of ultimate mineralization. It was found that the cumulative level of carbon dioxide evolved signifies the minimum extent of biodegradation of the substrate, and the oxygen consumed is a good indicator of the maximum extent of substrate degradation.Paper presented at the Biodegradable Materials and Packaging Conference, September 22–23, 1993, Natick, Massachusetts.     

Low or absent unconjugated estriol in pregnancy: an indicator for steroid sulfatase deficiency detectable by fluorescence in situ hybridization and biochemical analysis

It has been previously reported that a low or absent maternal serum unconjugated estriol (uE3) level is associated with placental steroid sulfatase (STS) deficiency. Here we report a correlation between patients who present with a very low or absent maternal serum uE3 and a deletion of the STS gene as assessed by fluorescence in situ hybridization (FISH). We studied nine prenatal cases that presented to the clinical laboratory with an abnormal triple screen, specifically low or absent maternal serum uE3 and a 46,XY karyotype. FISH analysis showed complete deletion of a probe containing the STS gene in six cases and one case had a partial deletion (reduced but not absent signal). The remaining two cases were not deleted for the STS probe. All mothers tested whose fetus showed a deletion were shown to be STS deletion carriers using FISH. Biochemical analysis was performed on 7/9 prenatal specimens. All fetuses deleted for the STS probe were also found to be deficient for STS by biochemical analysis of cultured amniotic fluid (5/5). Of the two fetuses not deleted for the STS probe, one was deficient for STS activity, while the other had a normal result. The abnormal result of enzyme deficiency by biochemical analysis in a non-deletion case likely represents a mutation in the STS gene, not detectable by this FISH assay. Postnatal FISH confirmation of the STS deletion was performed in 1/7 cases. Clinical follow-up was available for 4/9 cases following birth. Copyright © 2002 John Wiley & Sons, Ltd.     

Pulmonary function changes in Chinese hamsters exposed six months to diesel exhaust

Chinese hamsters were exposed for eight hours per day to automotive diesel exhaust emissions which were diluted with air (18 to 1) and had a particulate level of 6.4 mg/m³. Pulmonary function measurements were made after six months exposure. Body weight (BW), lung weight (LW), vital capacity (VC), residual volume by water displacement (RV_w) and by gas dilution (RV_D), alveolar volume (V_A), and carbon monoxide transfer factor (D_LCO) were measured. LW showed a significant increase in the diesel exposed animals (P < 0.01) while VC, RV_W, and D_LCO showed decreases (P < 0.01). Static deflation volume-pressure curves showed depressed deflation volumes for diesel exposed animals when volumes were corrected for body weight and even greater depressed volumes when volumes were corrected for lung weight. However, when volumes were expressed as percent vital capacity, the diesel exposed animals had higher lung volumes at 0 and 5 cm H₂O. Results of the pathological examination of the lung tissue will be necessary for final analysis of our findings. However, preliminary interpretation indicates possible emphysematous changes which are compatible with the observed decrease in D_LCO.     

Modeling the annual thermal regime of lake Ohrid,Yugoslavia, using daily weather data

The time-dependent characteristics of the five weather variables which control the annual thermal response of Lake Ohrid are analyzed in detail. These are daily values for solar radiation, air temperature, humidity, wind speed and cloud cover. A simple numerical model of the lake's thermal response, forced by thermally driven density mixing, is developed and tested using observed thermal profiles for verification. The numerical model successfully reproduces the major features of the lake's thermal regime over a 6 y period from 1972 to 1977, the average root mean square value for the simulated profiles being 1.2°C with extremes of 2.2 and 0.3°C and a standard deviation of 0.4°C.     

The evolutionary continuum of limb function from early theropods to birds

The bipedal stance and gait of theropod dinosaurs evolved gradually along the lineage leading to birds and at some point(s), flight evolved. How and when did these changes occur? We review the evidence from neontology and palaeontology, including pectoral and pelvic limb functional morphology, fossil footprints/trackways and biomechanical models and simulations. We emphasise that many false dichotomies or categories have been applied to theropod form and function, and sometimes, these impede research progress. For example, dichotomisation of locomotor function into ‘non-avian’ and ‘avian’ modes is only a conceptual crutch; the evidence supports a continuous transition. Simplification of pelvic limb function into cursorial/non-cursorial morphologies or flexed/columnar poses has outlived its utility. For the pectoral limbs, even the classic predatory strike vs. flight wing-stroke distinction and separation of theropods into non-flying and flying—or terrestrial and arboreal—categories may be missing important subtleties. Distinguishing locomotor function between taxa, even with quantitative approaches, will always be fraught with ambiguity, making it difficult to find real differences if that ambiguity is properly acknowledged. There must be an ‘interpretive asymptote’ for reconstructing dinosaur limb function that available methods and evidence cannot overcome. We may be close to that limit, but how far can it be stretched with improved methods and evidence, if at all? The way forward is a combination of techniques that emphasises integration of neontological and palaeontological evidence and quantitative assessment of limb function cautiously applied with validated techniques and sensitivity analysis of unknown variables.     

Ecology of juvenile hawksbills (Eretmochelys imbricata) at Buck Island Reef National Monument, US Virgin Islands

Surveys of juvenile hawksbills around Buck Island Reef National Monument, US Virgin Islands from 1994 to 1999 revealed distributional patterns and resulted in a total of 75 individual hawksbill captures from all years; turtles ranged from 23.2 to 77.7 cm curved carapace length (CCL; mean 42.1 ± 12.3 cm SD). Juveniles concentrated where Zoanthid cover was highest. Length of time between recaptures, or presumed minimum site residency, ranged from 59 to 1,396 days (mean 620.8 ± 402.4 days SD). Growth rates for 23 juveniles ranged from 0.0 to 9.5 cm year^?1 (mean 4.1 ± 2.4 cm year^?1SD). Annual mean growth rates were non-monotonic, with the largest mean growth rate occurring in the 30–39 cm CCL size class. Gastric lavages indicated that Zoanthids were the primary food source for hawksbills. These results contribute to our understanding of juvenile hawksbill ecology and serve as a baseline for future studies or inventories of hawksbills in the Caribbean.     

Pleistocene diversification of the Pomacentrus coelestis species complex (Pisces: Pomacentridae): historical biogeography and species boundaries

Pleistocene eustatic changes in sea level are often invoked to explain genetic divergence among marine organisms. However, molecular phylogenies have revealed relatively few examples of speciation events dating to the Pleistocene. We present a species-level hypothesis of the timing of evolution for the Pomacentrus coelestis species complex (Pomacentridae), based on the nuclear S7 intron and the mitochondrial Cytb gene, and reconstruct ancestral range distributions across the timetree. Ancestral range reconstruction suggests the complex originated in the Coral Triangle and East Indian Ocean, with subsequent range expansion outward from this region. We suggest that land barriers to dispersal (e.g., Indo-Pacific barrier) may be responsible for the divergence between Indian (P. alleni, P. similis, P. caeruleopunctatus, and P. caeruleus) and Pacific (P. micronesicus, P. auriventris, and P. coelestis) species groups, and subsequent isolation by Pleistocene sea-level fluctuations in certain areas of the Coral Triangle (glacial refugia) may play an important role in the diversification of this species complex. Additionally, our analyses show cryptic lineages within P. micronesicus and highlight the need for comprehensive sampling within and among species to reveal recent speciation events.     

Conceptual Framework of Connectivity for a National Agroecosystem Model Based on Transport Processes and Management Practices

There is a critical need for a national agroecosystem model for conservation policy and environmental planning, driven by issues including harmful algal blooms, water scarcity, flooding, and other weather‐related extremes. In this study, we illustrate the feasibility of a national agroecosystem model that will downscale processes to individual fields and first‐order channels. We propose to conceptually divide the conterminous United States (U.S.) into process domains as a framework for simulating processes and management at relevant scales. Specifically, we are proposing five domains: field (1–50 ha), transition (0.2–2.0 km²), headwater (1–15 km²), tributaries (15–150 km²), and main river (>150 km²). The proposed conceptual framework hydrologically connects fields across the U.S. using the National Hydrography Dataset (NHDPlus version 2). Parameterizing the Soil and Water Assessment Tool for the national agroecosystem model resulted in 4,880,000 agricultural fields, 2,250,000 non‐agricultural hydrologic response units, and 7,130,000 transition, 1,610,000 headwater, 591,000 tributary, and 432,400 main channels. Application of this framework was shown for Hydrologic Unit Code 07120002 in central Illinois and Indiana to demonstrate the feasibility of the approach using data that is readily available across the U.S. The new connectivity framework has the potential to dramatically improve national conservation and environmental assessments performed by U.S. Department of Agriculture and U.S. Environmental Protection Agency.