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Effects of specimen handling and otolith preparation on concentration of elements in fish otoliths 总被引:2,自引:0,他引:2
The use of otolith chemistry as a tool for analysing the biology of fishes assumes that the procedures used to collect and
prepare otoliths for analysis do not alter their composition. With otoliths of Nemadactylus macropterus, Hoplostethus atlanticus, and Rhombosolea tapirina, we show that this assumption is not valid for the elements that can be detected using electron-probe microanalysers (those
present at concentrations greater than ≃100 parts per million): all six elements routinely measured using these techniques
were affected by at least one post-mortem procedure tested. Measured concentrations of calcium and strontium were relatively
insensitive to most procedures tested, whereas concentrations of sodium, potassium, sulphur and chlorine were affected substantially
by many commonly used procedures. The ease with which otolith composition could be modified post-mortem suggests that apparent
geographic, habitat-specific or ontogenetic differences in otolith composition should be interpreted with extreme caution
because of easily induced artefacts and the problem of pseudo-replication associated with the ways otoliths are sampled and
prepared for analysis.
Received: 23 September 1996 / Accepted: 11 April 1998 相似文献
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Dirk J. Roux Richard J. Stirzaker Charles M. Breen E.C. Lefroy Hamish P. Cresswell 《Environmental Science & Policy》2010,13(8):733-741
In response to the increasingly complex social–ecological issues facing society, there is a growing trend to conduct environmental research in large collaborative programs. This approach is described as transdisciplinary research as it transcends formal disciplinary boundaries, explicitly acknowledges that many different perspectives are relevant to the resolution of complex problems, and actively involves the users of research. This poses challenges for the evaluation of “impact” as any evaluation process must take into consideration the different expectations, values, culture, language and reward structures of the main participating groups, the funders, researchers and end users. How can these participating groups learn about the progress of a transdisciplinary research program in a way that is purposeful and structured, continues through the life of the program, and includes explicit feedback mechanisms that facilitate adaptation during the course of the program? This paper presents a framework for co-reflecting on the accomplishment of transdisciplinary research programs. The framework incorporates the perspectives of funders, researchers and users, and recognizes that while they place different emphasis on measures of achievement such as efficiency, rigor and relevance, ultimate accomplishment in terms of translating knowledge into practice requires that the needs and expectations of all three groups are adequately addressed. What emerges from the framework is the importance of early investment in processes, behaviors and relationships that foster social learning and the co-production of the knowledge and understanding that are required to ensure relevance; while maintaining emphasis in the traditional areas of formally testing evidence and mentoring young researchers to ensure rigor and build confidence and capacity in transdisciplinary approaches. 相似文献
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Genetic divergence and phylogenetic inferences in five species of Mugilidae (Pisces: Perciformes) 总被引:4,自引:0,他引:4
Allozyme electrophoresis was used to compare the genetic divergence of global populations of Mugil cephalus Linnaeus, 1758 and two congeneric [M.␣curema Cuvier & Valenciennes, 1836; M. gyrans (Jordan & Gilbert, 1885)] and two more distantly related [Liza ramada (Risso, 1826); Xenomugil thoburni (Jordan & Starks, 1896)] species on the basis of 18 enzyme loci. The amount of genetic divergence among the species examined
is in agreement with their present systematic status, the differences being larger among non-congeneric species than among
species of the same genus. Intraspecific genetic distances in M. cephalus (average Nei's D= 0.154), although higher than those reported for conspecific populations of fish, appear to be small when compared to the
interspecific values among mugilid species (0.821 ≤ Nei's D ≤ 1.744). Phylogenetic trees obtained by genetic distance methods and discrete character parsimony analysis were of similar
topology, except for the relationships within the genus Mugil and for the arrangement of M. cephalus populations.
Received: 7 April 1997 / Accepted: 4 February 1998 相似文献
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